Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine

Autores
Larrieu, Edmundo Juan; Poggio, Thelma Veronica; Mujica, Guillermo; Gauci, Charles G.; Labanchi, Jose Luis; Herrero, Eduardo; Araya, Daniel; Grizmado, Claudia; Calabro, Arnoldo; Talmon, Gabriel; Crowley, Pablo Ernesto; Santillán, Graciela; Vizcaychipi, Katherina; Seleiman, Marcos; Sepulveda, Luis; Arezo, Marcos; García Cachau, Mariela Alejandra; Lamberti, Roberto; Molina, Leonardo; Gino, Lilia Mabel; Donadeu, Meritxell; Lightowlers, Marshall W.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cystic echinococcosis is endemic in the Rio Negro province of Argentina and, for this, a control program using praziquantel in dogs was developed from 1980. The transmission rate to humans and sheep has decreased significantly, however transmission persists. In 2009 the vaccination of sheep with EG95 was incorporated in some areas of the province. The objective of the study was to evaluate the humoral responses to the vaccine EG95. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Blood samples from 6 vaccinated groups and 4 no vaccinated sheep were obtained for determination of antibody titles against EG95 protein. Anti-EG95 responses were determined as described by Heath and Koolaard, 2012. Responses were evaluated from 331 animals. Median ELISA absorbance values in vaccinated group was 0.828, and in non-vaccinated groups was 0.218. EG95 antibody responses in sheep from different cohorts of non-immunized control groups and vaccinated groups reveal the sustained increase in response seen in animals following the third immunization. (p< 0.0001). Significant differences are also evidenced in ANOVA test (p< 0.001). An anti-EG95 antibody response was induced in all groups of immunized sheep, 4 times higher (0.828) than the mean observed in the control groups (0.218). Data described here indicate that following a third vaccination with the EG95 vaccine at 1 year of age the specific IgG responses detected in the serum of sheep increased to a level greater than that seen following the second immunization and that this response was maintained longitudinally over time, for at least 5 years.
Fil: Larrieu, Edmundo Juan. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Negro; Argentina
Fil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
Fil: Mujica, Guillermo. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Gauci, Charles G.. University of Melbourne; Australia
Fil: Labanchi, Jose Luis. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Herrero, Eduardo. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Araya, Daniel. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Grizmado, Claudia. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Calabro, Arnoldo. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Talmon, Gabriel. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Crowley, Pablo Ernesto. Universidad Nacional de Río Negro; Argentina
Fil: Santillán, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Vizcaychipi, Katherina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Seleiman, Marcos. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Sepulveda, Luis. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Arezo, Marcos. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: García Cachau, Mariela Alejandra. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Lamberti, Roberto. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Molina, Leonardo. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Gino, Lilia Mabel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Donadeu, Meritxell. University of Melbourne; Australia
Fil: Lightowlers, Marshall W.. University of Melbourne; Australia
Materia
Echinococcosis
Vaccine Eg95
Sheep
Humoral Response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43582
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43582
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccineLarrieu, Edmundo JuanPoggio, Thelma VeronicaMujica, GuillermoGauci, Charles G.Labanchi, Jose LuisHerrero, EduardoAraya, DanielGrizmado, ClaudiaCalabro, ArnoldoTalmon, GabrielCrowley, Pablo ErnestoSantillán, GracielaVizcaychipi, KatherinaSeleiman, MarcosSepulveda, LuisArezo, MarcosGarcía Cachau, Mariela AlejandraLamberti, RobertoMolina, LeonardoGino, Lilia MabelDonadeu, MeritxellLightowlers, Marshall W.EchinococcosisVaccine Eg95SheepHumoral Responsehttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Cystic echinococcosis is endemic in the Rio Negro province of Argentina and, for this, a control program using praziquantel in dogs was developed from 1980. The transmission rate to humans and sheep has decreased significantly, however transmission persists. In 2009 the vaccination of sheep with EG95 was incorporated in some areas of the province. The objective of the study was to evaluate the humoral responses to the vaccine EG95. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Blood samples from 6 vaccinated groups and 4 no vaccinated sheep were obtained for determination of antibody titles against EG95 protein. Anti-EG95 responses were determined as described by Heath and Koolaard, 2012. Responses were evaluated from 331 animals. Median ELISA absorbance values in vaccinated group was 0.828, and in non-vaccinated groups was 0.218. EG95 antibody responses in sheep from different cohorts of non-immunized control groups and vaccinated groups reveal the sustained increase in response seen in animals following the third immunization. (p< 0.0001). Significant differences are also evidenced in ANOVA test (p< 0.001). An anti-EG95 antibody response was induced in all groups of immunized sheep, 4 times higher (0.828) than the mean observed in the control groups (0.218). Data described here indicate that following a third vaccination with the EG95 vaccine at 1 year of age the specific IgG responses detected in the serum of sheep increased to a level greater than that seen following the second immunization and that this response was maintained longitudinally over time, for at least 5 years.Fil: Larrieu, Edmundo Juan. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Negro; ArgentinaFil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; ArgentinaFil: Mujica, Guillermo. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Gauci, Charles G.. University of Melbourne; AustraliaFil: Labanchi, Jose Luis. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Herrero, Eduardo. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Araya, Daniel. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Grizmado, Claudia. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Calabro, Arnoldo. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Talmon, Gabriel. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Crowley, Pablo Ernesto. Universidad Nacional de Río Negro; ArgentinaFil: Santillán, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; ArgentinaFil: Vizcaychipi, Katherina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; ArgentinaFil: Seleiman, Marcos. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Sepulveda, Luis. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: Arezo, Marcos. Provincia de Río Negro. Ministerio de Salud; ArgentinaFil: García Cachau, Mariela Alejandra. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Lamberti, Roberto. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Molina, Leonardo. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Gino, Lilia Mabel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Donadeu, Meritxell. University of Melbourne; AustraliaFil: Lightowlers, Marshall W.. University of Melbourne; AustraliaElsevier Ireland2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43582Larrieu, Edmundo Juan; Poggio, Thelma Veronica; Mujica, Guillermo; Gauci, Charles G.; Labanchi, Jose Luis; et al.; Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine; Elsevier Ireland; Parasitology International; 66; 3; 6-2017; 258-2611383-5769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.parint.2017.01.020info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383576916302793info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:02Zoai:ri.conicet.gov.ar:11336/43582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:03.001CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
title Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
spellingShingle Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
Larrieu, Edmundo Juan
Echinococcosis
Vaccine Eg95
Sheep
Humoral Response
title_short Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
title_full Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
title_fullStr Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
title_full_unstemmed Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
title_sort Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine
dc.creator.none.fl_str_mv Larrieu, Edmundo Juan
Poggio, Thelma Veronica
Mujica, Guillermo
Gauci, Charles G.
Labanchi, Jose Luis
Herrero, Eduardo
Araya, Daniel
Grizmado, Claudia
Calabro, Arnoldo
Talmon, Gabriel
Crowley, Pablo Ernesto
Santillán, Graciela
Vizcaychipi, Katherina
Seleiman, Marcos
Sepulveda, Luis
Arezo, Marcos
García Cachau, Mariela Alejandra
Lamberti, Roberto
Molina, Leonardo
Gino, Lilia Mabel
Donadeu, Meritxell
Lightowlers, Marshall W.
author Larrieu, Edmundo Juan
author_facet Larrieu, Edmundo Juan
Poggio, Thelma Veronica
Mujica, Guillermo
Gauci, Charles G.
Labanchi, Jose Luis
Herrero, Eduardo
Araya, Daniel
Grizmado, Claudia
Calabro, Arnoldo
Talmon, Gabriel
Crowley, Pablo Ernesto
Santillán, Graciela
Vizcaychipi, Katherina
Seleiman, Marcos
Sepulveda, Luis
Arezo, Marcos
García Cachau, Mariela Alejandra
Lamberti, Roberto
Molina, Leonardo
Gino, Lilia Mabel
Donadeu, Meritxell
Lightowlers, Marshall W.
author_role author
author2 Poggio, Thelma Veronica
Mujica, Guillermo
Gauci, Charles G.
Labanchi, Jose Luis
Herrero, Eduardo
Araya, Daniel
Grizmado, Claudia
Calabro, Arnoldo
Talmon, Gabriel
Crowley, Pablo Ernesto
Santillán, Graciela
Vizcaychipi, Katherina
Seleiman, Marcos
Sepulveda, Luis
Arezo, Marcos
García Cachau, Mariela Alejandra
Lamberti, Roberto
Molina, Leonardo
Gino, Lilia Mabel
Donadeu, Meritxell
Lightowlers, Marshall W.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Echinococcosis
Vaccine Eg95
Sheep
Humoral Response
topic Echinococcosis
Vaccine Eg95
Sheep
Humoral Response
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cystic echinococcosis is endemic in the Rio Negro province of Argentina and, for this, a control program using praziquantel in dogs was developed from 1980. The transmission rate to humans and sheep has decreased significantly, however transmission persists. In 2009 the vaccination of sheep with EG95 was incorporated in some areas of the province. The objective of the study was to evaluate the humoral responses to the vaccine EG95. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Blood samples from 6 vaccinated groups and 4 no vaccinated sheep were obtained for determination of antibody titles against EG95 protein. Anti-EG95 responses were determined as described by Heath and Koolaard, 2012. Responses were evaluated from 331 animals. Median ELISA absorbance values in vaccinated group was 0.828, and in non-vaccinated groups was 0.218. EG95 antibody responses in sheep from different cohorts of non-immunized control groups and vaccinated groups reveal the sustained increase in response seen in animals following the third immunization. (p< 0.0001). Significant differences are also evidenced in ANOVA test (p< 0.001). An anti-EG95 antibody response was induced in all groups of immunized sheep, 4 times higher (0.828) than the mean observed in the control groups (0.218). Data described here indicate that following a third vaccination with the EG95 vaccine at 1 year of age the specific IgG responses detected in the serum of sheep increased to a level greater than that seen following the second immunization and that this response was maintained longitudinally over time, for at least 5 years.
Fil: Larrieu, Edmundo Juan. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Negro; Argentina
Fil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
Fil: Mujica, Guillermo. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Gauci, Charles G.. University of Melbourne; Australia
Fil: Labanchi, Jose Luis. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Herrero, Eduardo. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Araya, Daniel. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Grizmado, Claudia. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Calabro, Arnoldo. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Talmon, Gabriel. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Crowley, Pablo Ernesto. Universidad Nacional de Río Negro; Argentina
Fil: Santillán, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Vizcaychipi, Katherina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Departamento de Parasitología; Argentina
Fil: Seleiman, Marcos. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Sepulveda, Luis. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: Arezo, Marcos. Provincia de Río Negro. Ministerio de Salud; Argentina
Fil: García Cachau, Mariela Alejandra. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Lamberti, Roberto. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Molina, Leonardo. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Gino, Lilia Mabel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Donadeu, Meritxell. University of Melbourne; Australia
Fil: Lightowlers, Marshall W.. University of Melbourne; Australia
description Cystic echinococcosis is endemic in the Rio Negro province of Argentina and, for this, a control program using praziquantel in dogs was developed from 1980. The transmission rate to humans and sheep has decreased significantly, however transmission persists. In 2009 the vaccination of sheep with EG95 was incorporated in some areas of the province. The objective of the study was to evaluate the humoral responses to the vaccine EG95. Lambs received two vaccinations with the EG95 vaccine followed by a single booster injection when the animals were 1-1.5 years of age. Blood samples from 6 vaccinated groups and 4 no vaccinated sheep were obtained for determination of antibody titles against EG95 protein. Anti-EG95 responses were determined as described by Heath and Koolaard, 2012. Responses were evaluated from 331 animals. Median ELISA absorbance values in vaccinated group was 0.828, and in non-vaccinated groups was 0.218. EG95 antibody responses in sheep from different cohorts of non-immunized control groups and vaccinated groups reveal the sustained increase in response seen in animals following the third immunization. (p< 0.0001). Significant differences are also evidenced in ANOVA test (p< 0.001). An anti-EG95 antibody response was induced in all groups of immunized sheep, 4 times higher (0.828) than the mean observed in the control groups (0.218). Data described here indicate that following a third vaccination with the EG95 vaccine at 1 year of age the specific IgG responses detected in the serum of sheep increased to a level greater than that seen following the second immunization and that this response was maintained longitudinally over time, for at least 5 years.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43582
Larrieu, Edmundo Juan; Poggio, Thelma Veronica; Mujica, Guillermo; Gauci, Charles G.; Labanchi, Jose Luis; et al.; Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine; Elsevier Ireland; Parasitology International; 66; 3; 6-2017; 258-261
1383-5769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43582
identifier_str_mv Larrieu, Edmundo Juan; Poggio, Thelma Veronica; Mujica, Guillermo; Gauci, Charles G.; Labanchi, Jose Luis; et al.; Pilot field trial of the EG95 vaccine against ovine cystic echinococcosis in Rio Negro, Argentina: Humoral response to the vaccine; Elsevier Ireland; Parasitology International; 66; 3; 6-2017; 258-261
1383-5769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.parint.2017.01.020
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383576916302793
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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