Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH

Autores
Ferreri, Natalia Analia; Eliades, Lorena Alejandra; Saparrat, Mario Carlos Nazareno; Ledesma, Tamara Melina; Russo, Maria Leticia; Cabello, Marta Noemí
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar medium. Growth and enzyme ability of selected fungi were also evaluated in agar media with 0.5 % casein, 1% Tween®20, and Chitin-Azure® by the production of a halo/growth ratio. We identified 22 fungal species using both methods. Since the two isolation methods added information to one another, both ones are necessary to recover the cultivable mycobiota associated with the exoskeleton debris. All fungi showed greater levels of enzyme activity in alkaline than acid medium with Tween®20. The halo diameter on casein and chitin varied according to the fungal isolate and pH. Most fungi had a larger halo at 4°C than at the other temperatures tested. Clonostachys rosea showed the greatest activity in all media at 4ºC. We conclude that exoskeletons of the N. granulata are a source of fungi able to produce enzyme activities that show differences upon incubation conditions to which they are cultivated such as ones including specific temperatures and pH values.
Fil: Ferreri, Natalia Analia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Ledesma, Tamara Melina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Materia
FUNGAL DESCOMPOSERS
DIVERSITY
CRAB EXOSKELOTONS
ENZYME ABILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/164205
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pHFerreri, Natalia AnaliaEliades, Lorena AlejandraSaparrat, Mario Carlos NazarenoLedesma, Tamara MelinaRusso, Maria LeticiaCabello, Marta NoemíFUNGAL DESCOMPOSERSDIVERSITYCRAB EXOSKELOTONSENZYME ABILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar medium. Growth and enzyme ability of selected fungi were also evaluated in agar media with 0.5 % casein, 1% Tween®20, and Chitin-Azure® by the production of a halo/growth ratio. We identified 22 fungal species using both methods. Since the two isolation methods added information to one another, both ones are necessary to recover the cultivable mycobiota associated with the exoskeleton debris. All fungi showed greater levels of enzyme activity in alkaline than acid medium with Tween®20. The halo diameter on casein and chitin varied according to the fungal isolate and pH. Most fungi had a larger halo at 4°C than at the other temperatures tested. Clonostachys rosea showed the greatest activity in all media at 4ºC. We conclude that exoskeletons of the N. granulata are a source of fungi able to produce enzyme activities that show differences upon incubation conditions to which they are cultivated such as ones including specific temperatures and pH values.Fil: Ferreri, Natalia Analia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Ledesma, Tamara Melina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaAcademia Brasileira de Ciencias2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164205Ferreri, Natalia Analia; Eliades, Lorena Alejandra; Saparrat, Mario Carlos Nazareno; Ledesma, Tamara Melina; Russo, Maria Leticia; et al.; Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 6-2021; 1-240001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202120201159info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/SLvstVSXGBxC5qK7Rcb8Ybk/?lang=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:58:23Zoai:ri.conicet.gov.ar:11336/164205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:58:23.724CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
title Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
spellingShingle Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
Ferreri, Natalia Analia
FUNGAL DESCOMPOSERS
DIVERSITY
CRAB EXOSKELOTONS
ENZYME ABILITY
title_short Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
title_full Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
title_fullStr Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
title_full_unstemmed Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
title_sort Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH
dc.creator.none.fl_str_mv Ferreri, Natalia Analia
Eliades, Lorena Alejandra
Saparrat, Mario Carlos Nazareno
Ledesma, Tamara Melina
Russo, Maria Leticia
Cabello, Marta Noemí
author Ferreri, Natalia Analia
author_facet Ferreri, Natalia Analia
Eliades, Lorena Alejandra
Saparrat, Mario Carlos Nazareno
Ledesma, Tamara Melina
Russo, Maria Leticia
Cabello, Marta Noemí
author_role author
author2 Eliades, Lorena Alejandra
Saparrat, Mario Carlos Nazareno
Ledesma, Tamara Melina
Russo, Maria Leticia
Cabello, Marta Noemí
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FUNGAL DESCOMPOSERS
DIVERSITY
CRAB EXOSKELOTONS
ENZYME ABILITY
topic FUNGAL DESCOMPOSERS
DIVERSITY
CRAB EXOSKELOTONS
ENZYME ABILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar medium. Growth and enzyme ability of selected fungi were also evaluated in agar media with 0.5 % casein, 1% Tween®20, and Chitin-Azure® by the production of a halo/growth ratio. We identified 22 fungal species using both methods. Since the two isolation methods added information to one another, both ones are necessary to recover the cultivable mycobiota associated with the exoskeleton debris. All fungi showed greater levels of enzyme activity in alkaline than acid medium with Tween®20. The halo diameter on casein and chitin varied according to the fungal isolate and pH. Most fungi had a larger halo at 4°C than at the other temperatures tested. Clonostachys rosea showed the greatest activity in all media at 4ºC. We conclude that exoskeletons of the N. granulata are a source of fungi able to produce enzyme activities that show differences upon incubation conditions to which they are cultivated such as ones including specific temperatures and pH values.
Fil: Ferreri, Natalia Analia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Ledesma, Tamara Melina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
description This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar medium. Growth and enzyme ability of selected fungi were also evaluated in agar media with 0.5 % casein, 1% Tween®20, and Chitin-Azure® by the production of a halo/growth ratio. We identified 22 fungal species using both methods. Since the two isolation methods added information to one another, both ones are necessary to recover the cultivable mycobiota associated with the exoskeleton debris. All fungi showed greater levels of enzyme activity in alkaline than acid medium with Tween®20. The halo diameter on casein and chitin varied according to the fungal isolate and pH. Most fungi had a larger halo at 4°C than at the other temperatures tested. Clonostachys rosea showed the greatest activity in all media at 4ºC. We conclude that exoskeletons of the N. granulata are a source of fungi able to produce enzyme activities that show differences upon incubation conditions to which they are cultivated such as ones including specific temperatures and pH values.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164205
Ferreri, Natalia Analia; Eliades, Lorena Alejandra; Saparrat, Mario Carlos Nazareno; Ledesma, Tamara Melina; Russo, Maria Leticia; et al.; Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 6-2021; 1-24
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164205
identifier_str_mv Ferreri, Natalia Analia; Eliades, Lorena Alejandra; Saparrat, Mario Carlos Nazareno; Ledesma, Tamara Melina; Russo, Maria Leticia; et al.; Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh: In vitro enzyme ability at different temperatures and pH; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 6-2021; 1-24
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202120201159
info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/SLvstVSXGBxC5qK7Rcb8Ybk/?lang=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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