Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis

Autores
Farrukh, Aleeza; Ortega, Felipe; Fan, Wenqiang; Marichal, Nicolás; Paez, Julieta Irene; Berninger, Benedikt; del Campo, Aránzazu; Salierno, Marcelo Javier
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties. In this article, Farrukh and colleagues show that bifunctionalization of hydrogel substrates with polylysine and a 19-mer peptide containing the laminin motif IKVAV promotes neurogenesis from embryonic neuroblasts and adult neural stem cells. Neurogenesis and neurite outgrowth can be further optimized by adjusting gel stiffness in a cell-type-specific manner.
Fil: Farrukh, Aleeza. Leibniz Institute for New Material; Alemania
Fil: Ortega, Felipe. Hospital Clínico San Carlos; España. Institute of Neurochemistry; España. Universidad Complutense de Madrid; España
Fil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Marichal, Nicolás. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Paez, Julieta Irene. Leibniz Institute for New Material; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berninger, Benedikt. Johannes Gutenberg Universitat Mainz; Alemania
Fil: del Campo, Aránzazu. Universitat Saarland; Alemania. Leibniz Institute for New Material; Alemania
Fil: Salierno, Marcelo Javier. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BIOENGINEERING
BIOMATERIALS
CELL DIFFERENTIATION
HYDROGELS
IKVAV
LAMININ
NEURAL STEM CELLS
NEUROGENESIS
POLYLYSINE
Β1-INTEGRIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102622
Acceder
id CONICETDig_99e744635570bd9060d9e2eae8bea92b
oai_identifier_str oai:ri.conicet.gov.ar:11336/102622
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote NeurogenesisFarrukh, AleezaOrtega, FelipeFan, WenqiangMarichal, NicolásPaez, Julieta IreneBerninger, Benediktdel Campo, AránzazuSalierno, Marcelo JavierBIOENGINEERINGBIOMATERIALSCELL DIFFERENTIATIONHYDROGELSIKVAVLAMININNEURAL STEM CELLSNEUROGENESISPOLYLYSINEΒ1-INTEGRINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties. In this article, Farrukh and colleagues show that bifunctionalization of hydrogel substrates with polylysine and a 19-mer peptide containing the laminin motif IKVAV promotes neurogenesis from embryonic neuroblasts and adult neural stem cells. Neurogenesis and neurite outgrowth can be further optimized by adjusting gel stiffness in a cell-type-specific manner.Fil: Farrukh, Aleeza. Leibniz Institute for New Material; AlemaniaFil: Ortega, Felipe. Hospital Clínico San Carlos; España. Institute of Neurochemistry; España. Universidad Complutense de Madrid; EspañaFil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Marichal, Nicolás. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Paez, Julieta Irene. Leibniz Institute for New Material; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berninger, Benedikt. Johannes Gutenberg Universitat Mainz; AlemaniaFil: del Campo, Aránzazu. Universitat Saarland; Alemania. Leibniz Institute for New Material; AlemaniaFil: Salierno, Marcelo Javier. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCell Press2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102622Farrukh, Aleeza; Ortega, Felipe; Fan, Wenqiang; Marichal, Nicolás; Paez, Julieta Irene; et al.; Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis; Cell Press; Stem Cell Reports; 9; 5; 11-2017; 1432-14402213-6711CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.stemcr.2017.09.002info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(17)30385-5info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829305/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:46Zoai:ri.conicet.gov.ar:11336/102622instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:46.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
title Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
spellingShingle Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
Farrukh, Aleeza
BIOENGINEERING
BIOMATERIALS
CELL DIFFERENTIATION
HYDROGELS
IKVAV
LAMININ
NEURAL STEM CELLS
NEUROGENESIS
POLYLYSINE
Β1-INTEGRIN
title_short Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
title_full Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
title_fullStr Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
title_full_unstemmed Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
title_sort Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
dc.creator.none.fl_str_mv Farrukh, Aleeza
Ortega, Felipe
Fan, Wenqiang
Marichal, Nicolás
Paez, Julieta Irene
Berninger, Benedikt
del Campo, Aránzazu
Salierno, Marcelo Javier
author Farrukh, Aleeza
author_facet Farrukh, Aleeza
Ortega, Felipe
Fan, Wenqiang
Marichal, Nicolás
Paez, Julieta Irene
Berninger, Benedikt
del Campo, Aránzazu
Salierno, Marcelo Javier
author_role author
author2 Ortega, Felipe
Fan, Wenqiang
Marichal, Nicolás
Paez, Julieta Irene
Berninger, Benedikt
del Campo, Aránzazu
Salierno, Marcelo Javier
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOENGINEERING
BIOMATERIALS
CELL DIFFERENTIATION
HYDROGELS
IKVAV
LAMININ
NEURAL STEM CELLS
NEUROGENESIS
POLYLYSINE
Β1-INTEGRIN
topic BIOENGINEERING
BIOMATERIALS
CELL DIFFERENTIATION
HYDROGELS
IKVAV
LAMININ
NEURAL STEM CELLS
NEUROGENESIS
POLYLYSINE
Β1-INTEGRIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties. In this article, Farrukh and colleagues show that bifunctionalization of hydrogel substrates with polylysine and a 19-mer peptide containing the laminin motif IKVAV promotes neurogenesis from embryonic neuroblasts and adult neural stem cells. Neurogenesis and neurite outgrowth can be further optimized by adjusting gel stiffness in a cell-type-specific manner.
Fil: Farrukh, Aleeza. Leibniz Institute for New Material; Alemania
Fil: Ortega, Felipe. Hospital Clínico San Carlos; España. Institute of Neurochemistry; España. Universidad Complutense de Madrid; España
Fil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Marichal, Nicolás. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Paez, Julieta Irene. Leibniz Institute for New Material; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berninger, Benedikt. Johannes Gutenberg Universitat Mainz; Alemania
Fil: del Campo, Aránzazu. Universitat Saarland; Alemania. Leibniz Institute for New Material; Alemania
Fil: Salierno, Marcelo Javier. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties. In this article, Farrukh and colleagues show that bifunctionalization of hydrogel substrates with polylysine and a 19-mer peptide containing the laminin motif IKVAV promotes neurogenesis from embryonic neuroblasts and adult neural stem cells. Neurogenesis and neurite outgrowth can be further optimized by adjusting gel stiffness in a cell-type-specific manner.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102622
Farrukh, Aleeza; Ortega, Felipe; Fan, Wenqiang; Marichal, Nicolás; Paez, Julieta Irene; et al.; Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis; Cell Press; Stem Cell Reports; 9; 5; 11-2017; 1432-1440
2213-6711
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102622
identifier_str_mv Farrukh, Aleeza; Ortega, Felipe; Fan, Wenqiang; Marichal, Nicolás; Paez, Julieta Irene; et al.; Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis; Cell Press; Stem Cell Reports; 9; 5; 11-2017; 1432-1440
2213-6711
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.stemcr.2017.09.002
info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(17)30385-5
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829305/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269818689945600
score 13.13397

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