Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands

Autores
Cabilla, Jimena Paula; Ronchetti, Sonia Alejandra; Nudler, Silvana Iris; Miler, Eliana Andrea; Quinteros, Alnilan Fernanda; Duvilanski, Beatriz Haydee
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
17β-Estradiol (E2) exerts inhibitory actions on the nitric oxide pathway in rat adult pituitary glands. Previously, we reported that in vivo E2 acute treatment had opposite effects on soluble guanylyl cyclase (sGC) subunits, increasing α1- and decreasing β1-subunit protein and mRNA expression and decreasing sGC activity in immature rats. Here we studied the E2 effect on sGC protein and mRNA expression in anterior pituitary gland from adult female rats to address whether the maturation of the hypothalamus-pituitary axis influences its effects and to corroborate whether these effects occur in physiological conditions such as during estrous cycle. E2 administration causes the same effect on sGC as seen in immature rats, and these effects are estrogen receptor dependent. These results suggest that E2 is the main effector of these changes. Since the sGC α-subunit increases while the sGC activity decreases, we studied if other less active isoforms of the sGC α-subunit are expressed. Here we show for the first time that sGCα2 and sGCα2 inhibitory (α2i) isoforms are expressed in this gland, but only sGCα2i mRNA increased after E2 acute treatment. Finally, to test whether E2 effects take place under a physiological condition, sGC subunit expression was monitored over estrous cycle. sGCα1, -β1, and -α2i fluctuate along estrous cycle, and these changes are directly related with E2 level fluctuations rather than to NO level variations. These findings show that E2 physiologically regulates sGC expression and highlight a novel mechanism by which E2 downregulates sGC activity in rat anterior pituitary gland. Copyright © 2009 the American Physiological Society.
Fil: Cabilla, Jimena Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Ronchetti, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Nudler, Silvana Iris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Miler, Eliana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Quinteros, Alnilan Fernanda. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Duvilanski, Beatriz Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Materia
ESTROGEN
INHIBITORY SUBUNIT
SOLUBLE GUANYLYL CYCLASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/124740
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glandsCabilla, Jimena PaulaRonchetti, Sonia AlejandraNudler, Silvana IrisMiler, Eliana AndreaQuinteros, Alnilan FernandaDuvilanski, Beatriz HaydeeESTROGENINHIBITORY SUBUNITSOLUBLE GUANYLYL CYCLASEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/317β-Estradiol (E2) exerts inhibitory actions on the nitric oxide pathway in rat adult pituitary glands. Previously, we reported that in vivo E2 acute treatment had opposite effects on soluble guanylyl cyclase (sGC) subunits, increasing α1- and decreasing β1-subunit protein and mRNA expression and decreasing sGC activity in immature rats. Here we studied the E2 effect on sGC protein and mRNA expression in anterior pituitary gland from adult female rats to address whether the maturation of the hypothalamus-pituitary axis influences its effects and to corroborate whether these effects occur in physiological conditions such as during estrous cycle. E2 administration causes the same effect on sGC as seen in immature rats, and these effects are estrogen receptor dependent. These results suggest that E2 is the main effector of these changes. Since the sGC α-subunit increases while the sGC activity decreases, we studied if other less active isoforms of the sGC α-subunit are expressed. Here we show for the first time that sGCα2 and sGCα2 inhibitory (α2i) isoforms are expressed in this gland, but only sGCα2i mRNA increased after E2 acute treatment. Finally, to test whether E2 effects take place under a physiological condition, sGC subunit expression was monitored over estrous cycle. sGCα1, -β1, and -α2i fluctuate along estrous cycle, and these changes are directly related with E2 level fluctuations rather than to NO level variations. These findings show that E2 physiologically regulates sGC expression and highlight a novel mechanism by which E2 downregulates sGC activity in rat anterior pituitary gland. Copyright © 2009 the American Physiological Society.Fil: Cabilla, Jimena Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Ronchetti, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Nudler, Silvana Iris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Miler, Eliana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Quinteros, Alnilan Fernanda. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Duvilanski, Beatriz Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaAmerican Physiological Society2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124740Cabilla, Jimena Paula; Ronchetti, Sonia Alejandra; Nudler, Silvana Iris; Miler, Eliana Andrea; Quinteros, Alnilan Fernanda; et al.; Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 296; 4; 4-2009; 731-7370193-1849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpendo.90795.2008info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.90795.2008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:55:09Zoai:ri.conicet.gov.ar:11336/124740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:55:10.045CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
title Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
spellingShingle Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
Cabilla, Jimena Paula
ESTROGEN
INHIBITORY SUBUNIT
SOLUBLE GUANYLYL CYCLASE
title_short Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
title_full Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
title_fullStr Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
title_full_unstemmed Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
title_sort Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands
dc.creator.none.fl_str_mv Cabilla, Jimena Paula
Ronchetti, Sonia Alejandra
Nudler, Silvana Iris
Miler, Eliana Andrea
Quinteros, Alnilan Fernanda
Duvilanski, Beatriz Haydee
author Cabilla, Jimena Paula
author_facet Cabilla, Jimena Paula
Ronchetti, Sonia Alejandra
Nudler, Silvana Iris
Miler, Eliana Andrea
Quinteros, Alnilan Fernanda
Duvilanski, Beatriz Haydee
author_role author
author2 Ronchetti, Sonia Alejandra
Nudler, Silvana Iris
Miler, Eliana Andrea
Quinteros, Alnilan Fernanda
Duvilanski, Beatriz Haydee
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ESTROGEN
INHIBITORY SUBUNIT
SOLUBLE GUANYLYL CYCLASE
topic ESTROGEN
INHIBITORY SUBUNIT
SOLUBLE GUANYLYL CYCLASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv 17β-Estradiol (E2) exerts inhibitory actions on the nitric oxide pathway in rat adult pituitary glands. Previously, we reported that in vivo E2 acute treatment had opposite effects on soluble guanylyl cyclase (sGC) subunits, increasing α1- and decreasing β1-subunit protein and mRNA expression and decreasing sGC activity in immature rats. Here we studied the E2 effect on sGC protein and mRNA expression in anterior pituitary gland from adult female rats to address whether the maturation of the hypothalamus-pituitary axis influences its effects and to corroborate whether these effects occur in physiological conditions such as during estrous cycle. E2 administration causes the same effect on sGC as seen in immature rats, and these effects are estrogen receptor dependent. These results suggest that E2 is the main effector of these changes. Since the sGC α-subunit increases while the sGC activity decreases, we studied if other less active isoforms of the sGC α-subunit are expressed. Here we show for the first time that sGCα2 and sGCα2 inhibitory (α2i) isoforms are expressed in this gland, but only sGCα2i mRNA increased after E2 acute treatment. Finally, to test whether E2 effects take place under a physiological condition, sGC subunit expression was monitored over estrous cycle. sGCα1, -β1, and -α2i fluctuate along estrous cycle, and these changes are directly related with E2 level fluctuations rather than to NO level variations. These findings show that E2 physiologically regulates sGC expression and highlight a novel mechanism by which E2 downregulates sGC activity in rat anterior pituitary gland. Copyright © 2009 the American Physiological Society.
Fil: Cabilla, Jimena Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Ronchetti, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Nudler, Silvana Iris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Miler, Eliana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Quinteros, Alnilan Fernanda. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Duvilanski, Beatriz Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
description 17β-Estradiol (E2) exerts inhibitory actions on the nitric oxide pathway in rat adult pituitary glands. Previously, we reported that in vivo E2 acute treatment had opposite effects on soluble guanylyl cyclase (sGC) subunits, increasing α1- and decreasing β1-subunit protein and mRNA expression and decreasing sGC activity in immature rats. Here we studied the E2 effect on sGC protein and mRNA expression in anterior pituitary gland from adult female rats to address whether the maturation of the hypothalamus-pituitary axis influences its effects and to corroborate whether these effects occur in physiological conditions such as during estrous cycle. E2 administration causes the same effect on sGC as seen in immature rats, and these effects are estrogen receptor dependent. These results suggest that E2 is the main effector of these changes. Since the sGC α-subunit increases while the sGC activity decreases, we studied if other less active isoforms of the sGC α-subunit are expressed. Here we show for the first time that sGCα2 and sGCα2 inhibitory (α2i) isoforms are expressed in this gland, but only sGCα2i mRNA increased after E2 acute treatment. Finally, to test whether E2 effects take place under a physiological condition, sGC subunit expression was monitored over estrous cycle. sGCα1, -β1, and -α2i fluctuate along estrous cycle, and these changes are directly related with E2 level fluctuations rather than to NO level variations. These findings show that E2 physiologically regulates sGC expression and highlight a novel mechanism by which E2 downregulates sGC activity in rat anterior pituitary gland. Copyright © 2009 the American Physiological Society.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124740
Cabilla, Jimena Paula; Ronchetti, Sonia Alejandra; Nudler, Silvana Iris; Miler, Eliana Andrea; Quinteros, Alnilan Fernanda; et al.; Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 296; 4; 4-2009; 731-737
0193-1849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124740
identifier_str_mv Cabilla, Jimena Paula; Ronchetti, Sonia Alejandra; Nudler, Silvana Iris; Miler, Eliana Andrea; Quinteros, Alnilan Fernanda; et al.; Nitric oxide sensitive-guanylyl cyclase subunits expression changes during estrous cycle in anterior pituitary glands; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 296; 4; 4-2009; 731-737
0193-1849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpendo.90795.2008
info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.90795.2008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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