Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress

Autores
Neira, Flavia Judith; Torrecilla, Norma Mariana; Pennacchio, Gisela Erika; Soaje, Marta; Jahn, Graciela Alma; Seltzer, Alicia Mabel; Valdez, Susana Ruth
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Neonatal lesions in the brain have less severe effects than in adults due to the neuronal plasticity of the developing nervous system, although they can cause cognitive and behavioral sequelae. Previously, we found that neonatal hypoxia (NH) transiently affected the expression of proteins associated with synaptogenesis in certain brain areas. The intermingled neural circuits controlling both stress and anxiety suggest a strong relationship between stress experiences and anxiety in both healthy and pathological conditions. We evaluated the long-term effects of NH on anxiety parameters and in stress-induced hormone release in adult female (estrous day of rat cycle) and male rats. Sprague Dawley rats at 4 Post-Natal Day (PND) were exposed to an atmosphere of low oxygen level (6.5% O2 and 93.5% N2) for 70 min. 4PND control pups were exposed to normal oxygen levels (Co) for 70 min. The humidity and temperature conditions were controlled. Pups were then returned with their mother until weaned, and then they were allowed to grow. At 3 months of age, both groups of rats were subjected to two tests, Elevated Plus Maze (EPM) to measure anxiety parameters and a stressful challenge to determine hormone response to acute stress (exposure to ether vapors for 2 min). EPM reflected an unconditional aversion to heights and open spaces, an anxiogenic behavior. The hormonal response to stress included the release of pituitary prolactin (PRL), adrenal progesterone (P4), and adrenal corticosterone (CORT). Blood samples were collected before and after 5 min of stress exposure for serum hormone determinations by RIA. In the EPM test, both female and male hypoxic rats increased the number of entries to the open arms (OA) and the time spent in the OA compared to Co (P<0.05). The results obtained indicated an anxiolytic-related behavior induced by NH, that was higher in female than in male hypoxic rats. Basal levels (unstressed) of PRL and P4 in NH rats remained similar to Co ones in both sexes. Only in female rats, NH increased the basal levels of CORT compared Co rats (P<0.05). In female and male rats, the hormonal release of PRL, P4, and CORT induced by stress, were differentially affected by NH. Hypoxia attenuated the stress-induced PRL secretion in female rats (P<0.05) while this response was blocked in males. The release of CORT by stress was blunted in both sexes by NH. The release of P4 by stress was inhibited in NH female but it was preserved in male rats. In conclusion, the long-term effects of NH were influenced by sex. NH altered the anxiety levels and the hormonal response to stress in adulthood. The alterations caused by NH at the brain level could be influencing the appropriate response to situations of stress and anxiety in adulthood.
Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Torrecilla, Norma Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pennacchio, Gisela Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Seltzer, Alicia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes; Argentina
Fil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
XXXVII Annual Meeting ot the Cuyo Biology Society
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
NEONATAL HYPOXIA
ELEVATED PLUS MAZE
STRESS
PROLACTIN
CORTICOSTERONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230731
Acceder
id CONICETDig_99af7bd9978729267f9176f5210d7061
oai_identifier_str oai:ri.conicet.gov.ar:11336/230731
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stressNeira, Flavia JudithTorrecilla, Norma MarianaPennacchio, Gisela ErikaSoaje, MartaJahn, Graciela AlmaSeltzer, Alicia MabelValdez, Susana RuthNEONATAL HYPOXIAELEVATED PLUS MAZESTRESSPROLACTINCORTICOSTERONEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Neonatal lesions in the brain have less severe effects than in adults due to the neuronal plasticity of the developing nervous system, although they can cause cognitive and behavioral sequelae. Previously, we found that neonatal hypoxia (NH) transiently affected the expression of proteins associated with synaptogenesis in certain brain areas. The intermingled neural circuits controlling both stress and anxiety suggest a strong relationship between stress experiences and anxiety in both healthy and pathological conditions. We evaluated the long-term effects of NH on anxiety parameters and in stress-induced hormone release in adult female (estrous day of rat cycle) and male rats. Sprague Dawley rats at 4 Post-Natal Day (PND) were exposed to an atmosphere of low oxygen level (6.5% O2 and 93.5% N2) for 70 min. 4PND control pups were exposed to normal oxygen levels (Co) for 70 min. The humidity and temperature conditions were controlled. Pups were then returned with their mother until weaned, and then they were allowed to grow. At 3 months of age, both groups of rats were subjected to two tests, Elevated Plus Maze (EPM) to measure anxiety parameters and a stressful challenge to determine hormone response to acute stress (exposure to ether vapors for 2 min). EPM reflected an unconditional aversion to heights and open spaces, an anxiogenic behavior. The hormonal response to stress included the release of pituitary prolactin (PRL), adrenal progesterone (P4), and adrenal corticosterone (CORT). Blood samples were collected before and after 5 min of stress exposure for serum hormone determinations by RIA. In the EPM test, both female and male hypoxic rats increased the number of entries to the open arms (OA) and the time spent in the OA compared to Co (P<0.05). The results obtained indicated an anxiolytic-related behavior induced by NH, that was higher in female than in male hypoxic rats. Basal levels (unstressed) of PRL and P4 in NH rats remained similar to Co ones in both sexes. Only in female rats, NH increased the basal levels of CORT compared Co rats (P<0.05). In female and male rats, the hormonal release of PRL, P4, and CORT induced by stress, were differentially affected by NH. Hypoxia attenuated the stress-induced PRL secretion in female rats (P<0.05) while this response was blocked in males. The release of CORT by stress was blunted in both sexes by NH. The release of P4 by stress was inhibited in NH female but it was preserved in male rats. In conclusion, the long-term effects of NH were influenced by sex. NH altered the anxiety levels and the hormonal response to stress in adulthood. The alterations caused by NH at the brain level could be influencing the appropriate response to situations of stress and anxiety in adulthood.Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Torrecilla, Norma Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Pennacchio, Gisela Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Seltzer, Alicia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes; ArgentinaFil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaXXXVII Annual Meeting ot the Cuyo Biology SocietySan LuisArgentinaSociedad de Biología de CuyoTech Science PressPiezzi, Ramon Salvador2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230731Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress; XXXVII Annual Meeting ot the Cuyo Biology Society ; San Luis; Argentina; 2019; 24-240327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2021/07/2020-XXXVII-Scientific-Meeting-of-the-Cuyo-Biology-Society2.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:38Zoai:ri.conicet.gov.ar:11336/230731instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:38.645CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
title Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
spellingShingle Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
Neira, Flavia Judith
NEONATAL HYPOXIA
ELEVATED PLUS MAZE
STRESS
PROLACTIN
CORTICOSTERONE
title_short Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
title_full Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
title_fullStr Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
title_full_unstemmed Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
title_sort Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress
dc.creator.none.fl_str_mv Neira, Flavia Judith
Torrecilla, Norma Mariana
Pennacchio, Gisela Erika
Soaje, Marta
Jahn, Graciela Alma
Seltzer, Alicia Mabel
Valdez, Susana Ruth
author Neira, Flavia Judith
author_facet Neira, Flavia Judith
Torrecilla, Norma Mariana
Pennacchio, Gisela Erika
Soaje, Marta
Jahn, Graciela Alma
Seltzer, Alicia Mabel
Valdez, Susana Ruth
author_role author
author2 Torrecilla, Norma Mariana
Pennacchio, Gisela Erika
Soaje, Marta
Jahn, Graciela Alma
Seltzer, Alicia Mabel
Valdez, Susana Ruth
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Piezzi, Ramon Salvador
dc.subject.none.fl_str_mv NEONATAL HYPOXIA
ELEVATED PLUS MAZE
STRESS
PROLACTIN
CORTICOSTERONE
topic NEONATAL HYPOXIA
ELEVATED PLUS MAZE
STRESS
PROLACTIN
CORTICOSTERONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Neonatal lesions in the brain have less severe effects than in adults due to the neuronal plasticity of the developing nervous system, although they can cause cognitive and behavioral sequelae. Previously, we found that neonatal hypoxia (NH) transiently affected the expression of proteins associated with synaptogenesis in certain brain areas. The intermingled neural circuits controlling both stress and anxiety suggest a strong relationship between stress experiences and anxiety in both healthy and pathological conditions. We evaluated the long-term effects of NH on anxiety parameters and in stress-induced hormone release in adult female (estrous day of rat cycle) and male rats. Sprague Dawley rats at 4 Post-Natal Day (PND) were exposed to an atmosphere of low oxygen level (6.5% O2 and 93.5% N2) for 70 min. 4PND control pups were exposed to normal oxygen levels (Co) for 70 min. The humidity and temperature conditions were controlled. Pups were then returned with their mother until weaned, and then they were allowed to grow. At 3 months of age, both groups of rats were subjected to two tests, Elevated Plus Maze (EPM) to measure anxiety parameters and a stressful challenge to determine hormone response to acute stress (exposure to ether vapors for 2 min). EPM reflected an unconditional aversion to heights and open spaces, an anxiogenic behavior. The hormonal response to stress included the release of pituitary prolactin (PRL), adrenal progesterone (P4), and adrenal corticosterone (CORT). Blood samples were collected before and after 5 min of stress exposure for serum hormone determinations by RIA. In the EPM test, both female and male hypoxic rats increased the number of entries to the open arms (OA) and the time spent in the OA compared to Co (P<0.05). The results obtained indicated an anxiolytic-related behavior induced by NH, that was higher in female than in male hypoxic rats. Basal levels (unstressed) of PRL and P4 in NH rats remained similar to Co ones in both sexes. Only in female rats, NH increased the basal levels of CORT compared Co rats (P<0.05). In female and male rats, the hormonal release of PRL, P4, and CORT induced by stress, were differentially affected by NH. Hypoxia attenuated the stress-induced PRL secretion in female rats (P<0.05) while this response was blocked in males. The release of CORT by stress was blunted in both sexes by NH. The release of P4 by stress was inhibited in NH female but it was preserved in male rats. In conclusion, the long-term effects of NH were influenced by sex. NH altered the anxiety levels and the hormonal response to stress in adulthood. The alterations caused by NH at the brain level could be influencing the appropriate response to situations of stress and anxiety in adulthood.
Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Torrecilla, Norma Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pennacchio, Gisela Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Seltzer, Alicia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Villa Mercedes; Argentina
Fil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
XXXVII Annual Meeting ot the Cuyo Biology Society
San Luis
Argentina
Sociedad de Biología de Cuyo
description Neonatal lesions in the brain have less severe effects than in adults due to the neuronal plasticity of the developing nervous system, although they can cause cognitive and behavioral sequelae. Previously, we found that neonatal hypoxia (NH) transiently affected the expression of proteins associated with synaptogenesis in certain brain areas. The intermingled neural circuits controlling both stress and anxiety suggest a strong relationship between stress experiences and anxiety in both healthy and pathological conditions. We evaluated the long-term effects of NH on anxiety parameters and in stress-induced hormone release in adult female (estrous day of rat cycle) and male rats. Sprague Dawley rats at 4 Post-Natal Day (PND) were exposed to an atmosphere of low oxygen level (6.5% O2 and 93.5% N2) for 70 min. 4PND control pups were exposed to normal oxygen levels (Co) for 70 min. The humidity and temperature conditions were controlled. Pups were then returned with their mother until weaned, and then they were allowed to grow. At 3 months of age, both groups of rats were subjected to two tests, Elevated Plus Maze (EPM) to measure anxiety parameters and a stressful challenge to determine hormone response to acute stress (exposure to ether vapors for 2 min). EPM reflected an unconditional aversion to heights and open spaces, an anxiogenic behavior. The hormonal response to stress included the release of pituitary prolactin (PRL), adrenal progesterone (P4), and adrenal corticosterone (CORT). Blood samples were collected before and after 5 min of stress exposure for serum hormone determinations by RIA. In the EPM test, both female and male hypoxic rats increased the number of entries to the open arms (OA) and the time spent in the OA compared to Co (P<0.05). The results obtained indicated an anxiolytic-related behavior induced by NH, that was higher in female than in male hypoxic rats. Basal levels (unstressed) of PRL and P4 in NH rats remained similar to Co ones in both sexes. Only in female rats, NH increased the basal levels of CORT compared Co rats (P<0.05). In female and male rats, the hormonal release of PRL, P4, and CORT induced by stress, were differentially affected by NH. Hypoxia attenuated the stress-induced PRL secretion in female rats (P<0.05) while this response was blocked in males. The release of CORT by stress was blunted in both sexes by NH. The release of P4 by stress was inhibited in NH female but it was preserved in male rats. In conclusion, the long-term effects of NH were influenced by sex. NH altered the anxiety levels and the hormonal response to stress in adulthood. The alterations caused by NH at the brain level could be influencing the appropriate response to situations of stress and anxiety in adulthood.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230731
Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress; XXXVII Annual Meeting ot the Cuyo Biology Society ; San Luis; Argentina; 2019; 24-24
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230731
identifier_str_mv Long-term effects of neonatal hypoxia on anxiety-related behaviors and hormonal response to acute stress; XXXVII Annual Meeting ot the Cuyo Biology Society ; San Luis; Argentina; 2019; 24-24
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
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application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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