Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer

Autores
Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; Marshall, Guillermo Ricardo; Miklavcic, Damijan
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.
Fil: Sersa, Gregor. Institute of Oncology Ljubljana; Eslovenia
Fil: Teissie, Justin. Centre National de la Recherche Scientifique; Francia
Fil: Cemazar, Maja. Institute of Oncology Ljubljana; Eslovenia. University of Primorska; Eslovenia
Fil: Signori, Emanuela. Istituto di Farmacologia Traslazionale ; Italia
Fil: Kamensek, Urska. Institute of Oncology Ljubljana; Eslovenia
Fil: Marshall, Guillermo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física del Plasma. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física del Plasma; Argentina
Fil: Miklavcic, Damijan. University of Ljubljana; Eslovenia
Materia
Electroporation
Electrochemotherapy
Gene Electrotransfer
Vaccination
Abscopal Effect
Pivac 14
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19319
Acceder
id CONICETDig_9966b2e91132dc7ec32118e4c1bb0dae
oai_identifier_str oai:ri.conicet.gov.ar:11336/19319
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransferSersa, GregorTeissie, JustinCemazar, MajaSignori, EmanuelaKamensek, UrskaMarshall, Guillermo RicardoMiklavcic, DamijanElectroporationElectrochemotherapyGene ElectrotransferVaccinationAbscopal EffectPivac 14https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.Fil: Sersa, Gregor. Institute of Oncology Ljubljana; EsloveniaFil: Teissie, Justin. Centre National de la Recherche Scientifique; FranciaFil: Cemazar, Maja. Institute of Oncology Ljubljana; Eslovenia. University of Primorska; EsloveniaFil: Signori, Emanuela. Istituto di Farmacologia Traslazionale ; ItaliaFil: Kamensek, Urska. Institute of Oncology Ljubljana; EsloveniaFil: Marshall, Guillermo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física del Plasma. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física del Plasma; ArgentinaFil: Miklavcic, Damijan. University of Ljubljana; EsloveniaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19319Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; et al.; Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer; Springer; Cancer Immunology Immunotherapy; 64; 10; 10-2015; 1315-13270340-70041432-0851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00262-015-1724-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00262-015-1724-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:12Zoai:ri.conicet.gov.ar:11336/19319instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:12.651CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
title Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
spellingShingle Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
Sersa, Gregor
Electroporation
Electrochemotherapy
Gene Electrotransfer
Vaccination
Abscopal Effect
Pivac 14
title_short Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
title_full Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
title_fullStr Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
title_full_unstemmed Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
title_sort Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
dc.creator.none.fl_str_mv Sersa, Gregor
Teissie, Justin
Cemazar, Maja
Signori, Emanuela
Kamensek, Urska
Marshall, Guillermo Ricardo
Miklavcic, Damijan
author Sersa, Gregor
author_facet Sersa, Gregor
Teissie, Justin
Cemazar, Maja
Signori, Emanuela
Kamensek, Urska
Marshall, Guillermo Ricardo
Miklavcic, Damijan
author_role author
author2 Teissie, Justin
Cemazar, Maja
Signori, Emanuela
Kamensek, Urska
Marshall, Guillermo Ricardo
Miklavcic, Damijan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Electroporation
Electrochemotherapy
Gene Electrotransfer
Vaccination
Abscopal Effect
Pivac 14
topic Electroporation
Electrochemotherapy
Gene Electrotransfer
Vaccination
Abscopal Effect
Pivac 14
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.
Fil: Sersa, Gregor. Institute of Oncology Ljubljana; Eslovenia
Fil: Teissie, Justin. Centre National de la Recherche Scientifique; Francia
Fil: Cemazar, Maja. Institute of Oncology Ljubljana; Eslovenia. University of Primorska; Eslovenia
Fil: Signori, Emanuela. Istituto di Farmacologia Traslazionale ; Italia
Fil: Kamensek, Urska. Institute of Oncology Ljubljana; Eslovenia
Fil: Marshall, Guillermo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física del Plasma. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física del Plasma; Argentina
Fil: Miklavcic, Damijan. University of Ljubljana; Eslovenia
description Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19319
Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; et al.; Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer; Springer; Cancer Immunology Immunotherapy; 64; 10; 10-2015; 1315-1327
0340-7004
1432-0851
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19319
identifier_str_mv Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; et al.; Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer; Springer; Cancer Immunology Immunotherapy; 64; 10; 10-2015; 1315-1327
0340-7004
1432-0851
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00262-015-1724-2
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00262-015-1724-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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