Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
- Autores
- Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; Marshall, Guillermo Ricardo; Miklavcic, Damijan
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.
Fil: Sersa, Gregor. Institute of Oncology Ljubljana; Eslovenia
Fil: Teissie, Justin. Centre National de la Recherche Scientifique; Francia
Fil: Cemazar, Maja. Institute of Oncology Ljubljana; Eslovenia. University of Primorska; Eslovenia
Fil: Signori, Emanuela. Istituto di Farmacologia Traslazionale ; Italia
Fil: Kamensek, Urska. Institute of Oncology Ljubljana; Eslovenia
Fil: Marshall, Guillermo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física del Plasma. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física del Plasma; Argentina
Fil: Miklavcic, Damijan. University of Ljubljana; Eslovenia - Materia
-
Electroporation
Electrochemotherapy
Gene Electrotransfer
Vaccination
Abscopal Effect
Pivac 14 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/19319
Ver los metadatos del registro completo
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Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransferSersa, GregorTeissie, JustinCemazar, MajaSignori, EmanuelaKamensek, UrskaMarshall, Guillermo RicardoMiklavcic, DamijanElectroporationElectrochemotherapyGene ElectrotransferVaccinationAbscopal EffectPivac 14https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.Fil: Sersa, Gregor. Institute of Oncology Ljubljana; EsloveniaFil: Teissie, Justin. Centre National de la Recherche Scientifique; FranciaFil: Cemazar, Maja. Institute of Oncology Ljubljana; Eslovenia. University of Primorska; EsloveniaFil: Signori, Emanuela. Istituto di Farmacologia Traslazionale ; ItaliaFil: Kamensek, Urska. Institute of Oncology Ljubljana; EsloveniaFil: Marshall, Guillermo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física del Plasma. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física del Plasma; ArgentinaFil: Miklavcic, Damijan. University of Ljubljana; EsloveniaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19319Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; et al.; Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer; Springer; Cancer Immunology Immunotherapy; 64; 10; 10-2015; 1315-13270340-70041432-0851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00262-015-1724-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00262-015-1724-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:39Zoai:ri.conicet.gov.ar:11336/19319instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:40.013CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
title |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
spellingShingle |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer Sersa, Gregor Electroporation Electrochemotherapy Gene Electrotransfer Vaccination Abscopal Effect Pivac 14 |
title_short |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
title_full |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
title_fullStr |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
title_full_unstemmed |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
title_sort |
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer |
dc.creator.none.fl_str_mv |
Sersa, Gregor Teissie, Justin Cemazar, Maja Signori, Emanuela Kamensek, Urska Marshall, Guillermo Ricardo Miklavcic, Damijan |
author |
Sersa, Gregor |
author_facet |
Sersa, Gregor Teissie, Justin Cemazar, Maja Signori, Emanuela Kamensek, Urska Marshall, Guillermo Ricardo Miklavcic, Damijan |
author_role |
author |
author2 |
Teissie, Justin Cemazar, Maja Signori, Emanuela Kamensek, Urska Marshall, Guillermo Ricardo Miklavcic, Damijan |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Electroporation Electrochemotherapy Gene Electrotransfer Vaccination Abscopal Effect Pivac 14 |
topic |
Electroporation Electrochemotherapy Gene Electrotransfer Vaccination Abscopal Effect Pivac 14 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment. Fil: Sersa, Gregor. Institute of Oncology Ljubljana; Eslovenia Fil: Teissie, Justin. Centre National de la Recherche Scientifique; Francia Fil: Cemazar, Maja. Institute of Oncology Ljubljana; Eslovenia. University of Primorska; Eslovenia Fil: Signori, Emanuela. Istituto di Farmacologia Traslazionale ; Italia Fil: Kamensek, Urska. Institute of Oncology Ljubljana; Eslovenia Fil: Marshall, Guillermo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física del Plasma. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física del Plasma; Argentina Fil: Miklavcic, Damijan. University of Ljubljana; Eslovenia |
description |
Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/19319 Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; et al.; Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer; Springer; Cancer Immunology Immunotherapy; 64; 10; 10-2015; 1315-1327 0340-7004 1432-0851 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/19319 |
identifier_str_mv |
Sersa, Gregor; Teissie, Justin; Cemazar, Maja; Signori, Emanuela; Kamensek, Urska; et al.; Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer; Springer; Cancer Immunology Immunotherapy; 64; 10; 10-2015; 1315-1327 0340-7004 1432-0851 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00262-015-1724-2 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00262-015-1724-2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269109200355328 |
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13.13397 |