Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium

Autores
Rodríguez, Dolores; González Aseguinolaza, Gloria; Rodríguez, Juan R.; Vijayan, Aneesh; Gherardi, Maria Magdalena; Rueda, Paloma; Casal, J. Ignacio; Esteban, Mariano
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
With the aim to develop an efficient and cost-effective approach to control malaria, we have generated porcine parvoviruslike particles (PPV-VLPs) carrying the CD8 + T cell epitope (SYVPSAEQI) of the circumsporozoite (CS) protein from Plasmodium yoelii fused to the PPV VP2 capsid protein (PPV-PYCS), and tested in prime/boost protocols with poxvirus vectors for efficacy in a rodent malaria model. As a proof-of concept, we have characterized the anti-CS CD8 + T cell response elicited by these hybrid PPV-VLPs in BALB/c mice after immunizations with the protein PPV-PYCS administered alone or in combination with recombinant vaccinia virus (VACV) vectors from the Western Reserve (WR) and modified virus Ankara (MVA) strains expressing the entire P. yoelii CS protein. The results of different immunization protocols showed that the combination of PPV-PYCS prime/poxvirus boost was highly immunogenic, inducing specific CD8+ T cell responses to CS resulting in 95% reduction in liver stage parasites two days following sporozoite challenge. In contrast, neither the administration of PPVPYCS alone nor the immunization with the vectors given in the order poxvirus/VLPs was as effective. The immune profile induced by VLPs/MVA boost was associated with polyfunctional and effector memory CD8+ T cell responses. These findings highlight the use of recombinant parvovirus PPV-PYCS particles as priming agents and poxvirus vectors, like MVA, as booster to enhance specific CD8+ T cell responses to Plasmodium antigens and to control infection. These observations are relevant in the design of T cell-inducing vaccines against malaria
Fil: Rodríguez, Dolores. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: González Aseguinolaza, Gloria. CIMA; España
Fil: Rodríguez, Juan R.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Vijayan, Aneesh. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rueda, Paloma. INGENASA; España
Fil: Casal, J. Ignacio. INGENASA; España
Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Materia
MVA
vaccines
Malaria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106286
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/106286
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of PlasmodiumRodríguez, DoloresGonzález Aseguinolaza, GloriaRodríguez, Juan R.Vijayan, AneeshGherardi, Maria MagdalenaRueda, PalomaCasal, J. IgnacioEsteban, MarianoMVAvaccinesMalariahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1With the aim to develop an efficient and cost-effective approach to control malaria, we have generated porcine parvoviruslike particles (PPV-VLPs) carrying the CD8 + T cell epitope (SYVPSAEQI) of the circumsporozoite (CS) protein from Plasmodium yoelii fused to the PPV VP2 capsid protein (PPV-PYCS), and tested in prime/boost protocols with poxvirus vectors for efficacy in a rodent malaria model. As a proof-of concept, we have characterized the anti-CS CD8 + T cell response elicited by these hybrid PPV-VLPs in BALB/c mice after immunizations with the protein PPV-PYCS administered alone or in combination with recombinant vaccinia virus (VACV) vectors from the Western Reserve (WR) and modified virus Ankara (MVA) strains expressing the entire P. yoelii CS protein. The results of different immunization protocols showed that the combination of PPV-PYCS prime/poxvirus boost was highly immunogenic, inducing specific CD8+ T cell responses to CS resulting in 95% reduction in liver stage parasites two days following sporozoite challenge. In contrast, neither the administration of PPVPYCS alone nor the immunization with the vectors given in the order poxvirus/VLPs was as effective. The immune profile induced by VLPs/MVA boost was associated with polyfunctional and effector memory CD8+ T cell responses. These findings highlight the use of recombinant parvovirus PPV-PYCS particles as priming agents and poxvirus vectors, like MVA, as booster to enhance specific CD8+ T cell responses to Plasmodium antigens and to control infection. These observations are relevant in the design of T cell-inducing vaccines against malariaFil: Rodríguez, Dolores. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: González Aseguinolaza, Gloria. CIMA; EspañaFil: Rodríguez, Juan R.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Vijayan, Aneesh. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rueda, Paloma. INGENASA; EspañaFil: Casal, J. Ignacio. INGENASA; EspañaFil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaPublic Library of Science2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106286Rodríguez, Dolores; González Aseguinolaza, Gloria; Rodríguez, Juan R.; Vijayan, Aneesh; Gherardi, Maria Magdalena; et al.; Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium; Public Library of Science; Plos One; 7; 4; 4-2012; 1-10; e344451932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0034445info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034445info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:19Zoai:ri.conicet.gov.ar:11336/106286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:19.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
title Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
spellingShingle Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
Rodríguez, Dolores
MVA
vaccines
Malaria
title_short Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
title_full Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
title_fullStr Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
title_full_unstemmed Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
title_sort Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
dc.creator.none.fl_str_mv Rodríguez, Dolores
González Aseguinolaza, Gloria
Rodríguez, Juan R.
Vijayan, Aneesh
Gherardi, Maria Magdalena
Rueda, Paloma
Casal, J. Ignacio
Esteban, Mariano
author Rodríguez, Dolores
author_facet Rodríguez, Dolores
González Aseguinolaza, Gloria
Rodríguez, Juan R.
Vijayan, Aneesh
Gherardi, Maria Magdalena
Rueda, Paloma
Casal, J. Ignacio
Esteban, Mariano
author_role author
author2 González Aseguinolaza, Gloria
Rodríguez, Juan R.
Vijayan, Aneesh
Gherardi, Maria Magdalena
Rueda, Paloma
Casal, J. Ignacio
Esteban, Mariano
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MVA
vaccines
Malaria
topic MVA
vaccines
Malaria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv With the aim to develop an efficient and cost-effective approach to control malaria, we have generated porcine parvoviruslike particles (PPV-VLPs) carrying the CD8 + T cell epitope (SYVPSAEQI) of the circumsporozoite (CS) protein from Plasmodium yoelii fused to the PPV VP2 capsid protein (PPV-PYCS), and tested in prime/boost protocols with poxvirus vectors for efficacy in a rodent malaria model. As a proof-of concept, we have characterized the anti-CS CD8 + T cell response elicited by these hybrid PPV-VLPs in BALB/c mice after immunizations with the protein PPV-PYCS administered alone or in combination with recombinant vaccinia virus (VACV) vectors from the Western Reserve (WR) and modified virus Ankara (MVA) strains expressing the entire P. yoelii CS protein. The results of different immunization protocols showed that the combination of PPV-PYCS prime/poxvirus boost was highly immunogenic, inducing specific CD8+ T cell responses to CS resulting in 95% reduction in liver stage parasites two days following sporozoite challenge. In contrast, neither the administration of PPVPYCS alone nor the immunization with the vectors given in the order poxvirus/VLPs was as effective. The immune profile induced by VLPs/MVA boost was associated with polyfunctional and effector memory CD8+ T cell responses. These findings highlight the use of recombinant parvovirus PPV-PYCS particles as priming agents and poxvirus vectors, like MVA, as booster to enhance specific CD8+ T cell responses to Plasmodium antigens and to control infection. These observations are relevant in the design of T cell-inducing vaccines against malaria
Fil: Rodríguez, Dolores. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: González Aseguinolaza, Gloria. CIMA; España
Fil: Rodríguez, Juan R.. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Vijayan, Aneesh. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rueda, Paloma. INGENASA; España
Fil: Casal, J. Ignacio. INGENASA; España
Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
description With the aim to develop an efficient and cost-effective approach to control malaria, we have generated porcine parvoviruslike particles (PPV-VLPs) carrying the CD8 + T cell epitope (SYVPSAEQI) of the circumsporozoite (CS) protein from Plasmodium yoelii fused to the PPV VP2 capsid protein (PPV-PYCS), and tested in prime/boost protocols with poxvirus vectors for efficacy in a rodent malaria model. As a proof-of concept, we have characterized the anti-CS CD8 + T cell response elicited by these hybrid PPV-VLPs in BALB/c mice after immunizations with the protein PPV-PYCS administered alone or in combination with recombinant vaccinia virus (VACV) vectors from the Western Reserve (WR) and modified virus Ankara (MVA) strains expressing the entire P. yoelii CS protein. The results of different immunization protocols showed that the combination of PPV-PYCS prime/poxvirus boost was highly immunogenic, inducing specific CD8+ T cell responses to CS resulting in 95% reduction in liver stage parasites two days following sporozoite challenge. In contrast, neither the administration of PPVPYCS alone nor the immunization with the vectors given in the order poxvirus/VLPs was as effective. The immune profile induced by VLPs/MVA boost was associated with polyfunctional and effector memory CD8+ T cell responses. These findings highlight the use of recombinant parvovirus PPV-PYCS particles as priming agents and poxvirus vectors, like MVA, as booster to enhance specific CD8+ T cell responses to Plasmodium antigens and to control infection. These observations are relevant in the design of T cell-inducing vaccines against malaria
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106286
Rodríguez, Dolores; González Aseguinolaza, Gloria; Rodríguez, Juan R.; Vijayan, Aneesh; Gherardi, Maria Magdalena; et al.; Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium; Public Library of Science; Plos One; 7; 4; 4-2012; 1-10; e34445
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106286
identifier_str_mv Rodríguez, Dolores; González Aseguinolaza, Gloria; Rodríguez, Juan R.; Vijayan, Aneesh; Gherardi, Maria Magdalena; et al.; Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium; Public Library of Science; Plos One; 7; 4; 4-2012; 1-10; e34445
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0034445
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034445
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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