Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea

Autores
Bartolotta, S. A.; Scuteri, M. A.; Hick, A. S.; Palermo, Jorge Alejandro; Rodriguez Brasco, Maria Florencia; Hajdu, E.; Mothes, B.; Lerner, C.; Campos, M.; Carballo, Marta Ana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs.
Fil: Bartolotta, S. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Scuteri, M. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Universidad Nacional de Luján. Departamento de Tecnología; Argentina
Fil: Hick, A. S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Palermo, Jorge Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Rodriguez Brasco, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Hajdu, E.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Mothes, B.. Fundação Zoobotânica do Rio Grande do Sul; Brasil
Fil: Lerner, C.. Fundação Zoobotânica do Rio Grande do Sul; Brasil
Fil: Campos, M.. Fundação Zoobotânica do Rio Grande do Sul; Brasil
Fil: Carballo, Marta Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Materia
CELL PROLIFERATION KINETICS
CYTOTOXICITY
GENOTOXICITY
MARINE SPONGES
MITOTIC INDEX
SISTER CHROMATID EXCHANGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71161
Acceder
id CONICETDig_985200186decb7a64b5caae4499a2512
oai_identifier_str oai:ri.conicet.gov.ar:11336/71161
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South SeaBartolotta, S. A.Scuteri, M. A.Hick, A. S.Palermo, Jorge AlejandroRodriguez Brasco, Maria FlorenciaHajdu, E.Mothes, B.Lerner, C.Campos, M.Carballo, Marta AnaCELL PROLIFERATION KINETICSCYTOTOXICITYGENOTOXICITYMARINE SPONGESMITOTIC INDEXSISTER CHROMATID EXCHANGEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs.Fil: Bartolotta, S. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Scuteri, M. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Universidad Nacional de Luján. Departamento de Tecnología; ArgentinaFil: Hick, A. S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Palermo, Jorge Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Rodriguez Brasco, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Hajdu, E.. Universidade Federal do Rio de Janeiro; BrasilFil: Mothes, B.. Fundação Zoobotânica do Rio Grande do Sul; BrasilFil: Lerner, C.. Fundação Zoobotânica do Rio Grande do Sul; BrasilFil: Campos, M.. Fundação Zoobotânica do Rio Grande do Sul; BrasilFil: Carballo, Marta Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaElsevier Science2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71161Bartolotta, S. A.; Scuteri, M. A.; Hick, A. S.; Palermo, Jorge Alejandro; Rodriguez Brasco, Maria Florencia; et al.; Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 369; 2; 2-2009; 144-1470022-0981CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022098108005698info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2008.11.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:13:21Zoai:ri.conicet.gov.ar:11336/71161instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:13:21.671CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
spellingShingle Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
Bartolotta, S. A.
CELL PROLIFERATION KINETICS
CYTOTOXICITY
GENOTOXICITY
MARINE SPONGES
MITOTIC INDEX
SISTER CHROMATID EXCHANGE
title_short Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_full Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_fullStr Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_full_unstemmed Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_sort Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
dc.creator.none.fl_str_mv Bartolotta, S. A.
Scuteri, M. A.
Hick, A. S.
Palermo, Jorge Alejandro
Rodriguez Brasco, Maria Florencia
Hajdu, E.
Mothes, B.
Lerner, C.
Campos, M.
Carballo, Marta Ana
author Bartolotta, S. A.
author_facet Bartolotta, S. A.
Scuteri, M. A.
Hick, A. S.
Palermo, Jorge Alejandro
Rodriguez Brasco, Maria Florencia
Hajdu, E.
Mothes, B.
Lerner, C.
Campos, M.
Carballo, Marta Ana
author_role author
author2 Scuteri, M. A.
Hick, A. S.
Palermo, Jorge Alejandro
Rodriguez Brasco, Maria Florencia
Hajdu, E.
Mothes, B.
Lerner, C.
Campos, M.
Carballo, Marta Ana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL PROLIFERATION KINETICS
CYTOTOXICITY
GENOTOXICITY
MARINE SPONGES
MITOTIC INDEX
SISTER CHROMATID EXCHANGE
topic CELL PROLIFERATION KINETICS
CYTOTOXICITY
GENOTOXICITY
MARINE SPONGES
MITOTIC INDEX
SISTER CHROMATID EXCHANGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs.
Fil: Bartolotta, S. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Scuteri, M. A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Universidad Nacional de Luján. Departamento de Tecnología; Argentina
Fil: Hick, A. S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Palermo, Jorge Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Rodriguez Brasco, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Hajdu, E.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Mothes, B.. Fundação Zoobotânica do Rio Grande do Sul; Brasil
Fil: Lerner, C.. Fundação Zoobotânica do Rio Grande do Sul; Brasil
Fil: Campos, M.. Fundação Zoobotânica do Rio Grande do Sul; Brasil
Fil: Carballo, Marta Ana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
description Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71161
Bartolotta, S. A.; Scuteri, M. A.; Hick, A. S.; Palermo, Jorge Alejandro; Rodriguez Brasco, Maria Florencia; et al.; Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 369; 2; 2-2009; 144-147
0022-0981
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71161
identifier_str_mv Bartolotta, S. A.; Scuteri, M. A.; Hick, A. S.; Palermo, Jorge Alejandro; Rodriguez Brasco, Maria Florencia; et al.; Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 369; 2; 2-2009; 144-147
0022-0981
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022098108005698
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2008.11.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.928904

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