Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application

Autores
Rabasco-Vílchez, Laura; Bolívar, Araceli; Ruiz, María Julia; Harrazi, Narjes; Mounier, Jérôme; Medina, Luis M.; Pérez-Rodríguez, Fernando
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study explores tomato agri-food residues as sources of bacteria with bioprotective potential to enhance product shelf-life and safety. A total of 245 bacterial strains were isolated, comprising predominantly Pseudomonas (52%) and Bacillus (44%) spp., with lactic acid bacteria (LAB) present at lower levels (4%). The antimicrobial activity of these isolates was assessed against pathogenic and spoilage bacteria and phytopathogenic molds. Notably, the Bacillus isolate TRB1-7 exhibited moderate activity against L. monocytogenes (inhibition halo diameter: 10.64 mm), while Pseudomonas and LAB isolates showed limited or no inhibition. Antifungal assays highlighted significant antifungal potential for Bacillus isolates. Results showed that 16% and 15% of the 245 isolates inhibited F. oxysporum and C. acutatum growth, respectively. Nine of these isolates underwent acid-adaptation and were evaluated against the selected molds using Potato Dextrose Agar (PDA) at pH 4.0 to simulate tomato conditions. Only isolate BRZ3-2, identified as B. aerius, was adapted to acidic conditions and inhibited F. oxysporum by 25%. Experiments on tomato-based agar at the same pH showed no inhibition by Bacillus isolates. These results suggest that tomato microbiota harbors acid-tolerant Bacillus strains with potential for post-harvest bio-preservation. Further studies on strains TRB1-7 and BRZ3-2 are required to develop effective bioprotective applications.
Fil: Rabasco-Vílchez, Laura. Universidad de Córdoba; España
Fil: Bolívar, Araceli. Universidad de Córdoba; España
Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Harrazi, Narjes. Universidad de Cartago; Túnez
Fil: Mounier, Jérôme. Universite de Bretagne Occidentale; Francia
Fil: Medina, Luis M.. Universidad de Córdoba; España
Fil: Pérez-Rodríguez, Fernando. Universidad de Córdoba; España
Materia
BIO PRESERVATION
POST-HARVEST
SHELF-LIFE
ANTIMICROBIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278857
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system applicationRabasco-Vílchez, LauraBolívar, AraceliRuiz, María JuliaHarrazi, NarjesMounier, JérômeMedina, Luis M.Pérez-Rodríguez, FernandoBIO PRESERVATIONPOST-HARVESTSHELF-LIFEANTIMICROBIALhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2This study explores tomato agri-food residues as sources of bacteria with bioprotective potential to enhance product shelf-life and safety. A total of 245 bacterial strains were isolated, comprising predominantly Pseudomonas (52%) and Bacillus (44%) spp., with lactic acid bacteria (LAB) present at lower levels (4%). The antimicrobial activity of these isolates was assessed against pathogenic and spoilage bacteria and phytopathogenic molds. Notably, the Bacillus isolate TRB1-7 exhibited moderate activity against L. monocytogenes (inhibition halo diameter: 10.64 mm), while Pseudomonas and LAB isolates showed limited or no inhibition. Antifungal assays highlighted significant antifungal potential for Bacillus isolates. Results showed that 16% and 15% of the 245 isolates inhibited F. oxysporum and C. acutatum growth, respectively. Nine of these isolates underwent acid-adaptation and were evaluated against the selected molds using Potato Dextrose Agar (PDA) at pH 4.0 to simulate tomato conditions. Only isolate BRZ3-2, identified as B. aerius, was adapted to acidic conditions and inhibited F. oxysporum by 25%. Experiments on tomato-based agar at the same pH showed no inhibition by Bacillus isolates. These results suggest that tomato microbiota harbors acid-tolerant Bacillus strains with potential for post-harvest bio-preservation. Further studies on strains TRB1-7 and BRZ3-2 are required to develop effective bioprotective applications.Fil: Rabasco-Vílchez, Laura. Universidad de Córdoba; EspañaFil: Bolívar, Araceli. Universidad de Córdoba; EspañaFil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Harrazi, Narjes. Universidad de Cartago; TúnezFil: Mounier, Jérôme. Universite de Bretagne Occidentale; FranciaFil: Medina, Luis M.. Universidad de Córdoba; EspañaFil: Pérez-Rodríguez, Fernando. Universidad de Córdoba; EspañaMDPI2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278857Rabasco-Vílchez, Laura; Bolívar, Araceli; Ruiz, María Julia; Harrazi, Narjes; Mounier, Jérôme; et al.; Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application; MDPI; Foods; 14; 21; 10-2025; 1-192304-8158CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2304-8158/14/21/3713info:eu-repo/semantics/altIdentifier/doi/10.3390/foods14213713info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T13:14:56Zoai:ri.conicet.gov.ar:11336/278857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 13:14:56.865CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
title Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
spellingShingle Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
Rabasco-Vílchez, Laura
BIO PRESERVATION
POST-HARVEST
SHELF-LIFE
ANTIMICROBIAL
title_short Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
title_full Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
title_fullStr Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
title_full_unstemmed Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
title_sort Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application
dc.creator.none.fl_str_mv Rabasco-Vílchez, Laura
Bolívar, Araceli
Ruiz, María Julia
Harrazi, Narjes
Mounier, Jérôme
Medina, Luis M.
Pérez-Rodríguez, Fernando
author Rabasco-Vílchez, Laura
author_facet Rabasco-Vílchez, Laura
Bolívar, Araceli
Ruiz, María Julia
Harrazi, Narjes
Mounier, Jérôme
Medina, Luis M.
Pérez-Rodríguez, Fernando
author_role author
author2 Bolívar, Araceli
Ruiz, María Julia
Harrazi, Narjes
Mounier, Jérôme
Medina, Luis M.
Pérez-Rodríguez, Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIO PRESERVATION
POST-HARVEST
SHELF-LIFE
ANTIMICROBIAL
topic BIO PRESERVATION
POST-HARVEST
SHELF-LIFE
ANTIMICROBIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This study explores tomato agri-food residues as sources of bacteria with bioprotective potential to enhance product shelf-life and safety. A total of 245 bacterial strains were isolated, comprising predominantly Pseudomonas (52%) and Bacillus (44%) spp., with lactic acid bacteria (LAB) present at lower levels (4%). The antimicrobial activity of these isolates was assessed against pathogenic and spoilage bacteria and phytopathogenic molds. Notably, the Bacillus isolate TRB1-7 exhibited moderate activity against L. monocytogenes (inhibition halo diameter: 10.64 mm), while Pseudomonas and LAB isolates showed limited or no inhibition. Antifungal assays highlighted significant antifungal potential for Bacillus isolates. Results showed that 16% and 15% of the 245 isolates inhibited F. oxysporum and C. acutatum growth, respectively. Nine of these isolates underwent acid-adaptation and were evaluated against the selected molds using Potato Dextrose Agar (PDA) at pH 4.0 to simulate tomato conditions. Only isolate BRZ3-2, identified as B. aerius, was adapted to acidic conditions and inhibited F. oxysporum by 25%. Experiments on tomato-based agar at the same pH showed no inhibition by Bacillus isolates. These results suggest that tomato microbiota harbors acid-tolerant Bacillus strains with potential for post-harvest bio-preservation. Further studies on strains TRB1-7 and BRZ3-2 are required to develop effective bioprotective applications.
Fil: Rabasco-Vílchez, Laura. Universidad de Córdoba; España
Fil: Bolívar, Araceli. Universidad de Córdoba; España
Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Harrazi, Narjes. Universidad de Cartago; Túnez
Fil: Mounier, Jérôme. Universite de Bretagne Occidentale; Francia
Fil: Medina, Luis M.. Universidad de Córdoba; España
Fil: Pérez-Rodríguez, Fernando. Universidad de Córdoba; España
description This study explores tomato agri-food residues as sources of bacteria with bioprotective potential to enhance product shelf-life and safety. A total of 245 bacterial strains were isolated, comprising predominantly Pseudomonas (52%) and Bacillus (44%) spp., with lactic acid bacteria (LAB) present at lower levels (4%). The antimicrobial activity of these isolates was assessed against pathogenic and spoilage bacteria and phytopathogenic molds. Notably, the Bacillus isolate TRB1-7 exhibited moderate activity against L. monocytogenes (inhibition halo diameter: 10.64 mm), while Pseudomonas and LAB isolates showed limited or no inhibition. Antifungal assays highlighted significant antifungal potential for Bacillus isolates. Results showed that 16% and 15% of the 245 isolates inhibited F. oxysporum and C. acutatum growth, respectively. Nine of these isolates underwent acid-adaptation and were evaluated against the selected molds using Potato Dextrose Agar (PDA) at pH 4.0 to simulate tomato conditions. Only isolate BRZ3-2, identified as B. aerius, was adapted to acidic conditions and inhibited F. oxysporum by 25%. Experiments on tomato-based agar at the same pH showed no inhibition by Bacillus isolates. These results suggest that tomato microbiota harbors acid-tolerant Bacillus strains with potential for post-harvest bio-preservation. Further studies on strains TRB1-7 and BRZ3-2 are required to develop effective bioprotective applications.
publishDate 2025
dc.date.none.fl_str_mv 2025-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278857
Rabasco-Vílchez, Laura; Bolívar, Araceli; Ruiz, María Julia; Harrazi, Narjes; Mounier, Jérôme; et al.; Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application; MDPI; Foods; 14; 21; 10-2025; 1-19
2304-8158
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278857
identifier_str_mv Rabasco-Vílchez, Laura; Bolívar, Araceli; Ruiz, María Julia; Harrazi, Narjes; Mounier, Jérôme; et al.; Screening of microbial isolates from tomato plants (Solanum lycopersicum L.) for bioprotective potential: from isolation to food model system application; MDPI; Foods; 14; 21; 10-2025; 1-19
2304-8158
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2304-8158/14/21/3713
info:eu-repo/semantics/altIdentifier/doi/10.3390/foods14213713
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.11174

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