Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina

Autores
Rinland, Maria Emilia; Gomez, Marisa Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.
Fil: Rinland, Maria Emilia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Gomez, Marisa Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Materia
Onion Waste
Growth Patterns
Onion Culture Medium
Carbon Sources
Hydrolysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10495
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, ArgentinaRinland, Maria EmiliaGomez, Marisa InesOnion WasteGrowth PatternsOnion Culture MediumCarbon SourcesHydrolysishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.Fil: Rinland, Maria Emilia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Gomez, Marisa Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaSpringer2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10495Rinland, Maria Emilia; Gomez, Marisa Ines; Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina; Springer; World Journal of Microbiology; 31; 3; 1-2015; 487-4970959-3993enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11274-015-1803-8info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-015-1803-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:04:21Zoai:ri.conicet.gov.ar:11336/10495instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:04:22.145CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
title Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
spellingShingle Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
Rinland, Maria Emilia
Onion Waste
Growth Patterns
Onion Culture Medium
Carbon Sources
Hydrolysis
title_short Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
title_full Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
title_fullStr Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
title_full_unstemmed Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
title_sort Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina
dc.creator.none.fl_str_mv Rinland, Maria Emilia
Gomez, Marisa Ines
author Rinland, Maria Emilia
author_facet Rinland, Maria Emilia
Gomez, Marisa Ines
author_role author
author2 Gomez, Marisa Ines
author2_role author
dc.subject.none.fl_str_mv Onion Waste
Growth Patterns
Onion Culture Medium
Carbon Sources
Hydrolysis
topic Onion Waste
Growth Patterns
Onion Culture Medium
Carbon Sources
Hydrolysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.
Fil: Rinland, Maria Emilia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Gomez, Marisa Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
description Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10495
Rinland, Maria Emilia; Gomez, Marisa Ines; Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina; Springer; World Journal of Microbiology; 31; 3; 1-2015; 487-497
0959-3993
url http://hdl.handle.net/11336/10495
identifier_str_mv Rinland, Maria Emilia; Gomez, Marisa Ines; Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina; Springer; World Journal of Microbiology; 31; 3; 1-2015; 487-497
0959-3993
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11274-015-1803-8
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-015-1803-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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