Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms

Autores
Quinteros, Melisa de Los Ángeles; Galera, Ivana Laura Delia; Tolosa, Juan Martín; García Martínez, Joaquín Calixto; Páez, Paulina Laura; Paraje, María Gabriela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene) vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. The minimum inhibitory concentration (MIC) for the planktonic population and the sessileminimum inhibitory concentrations (SMIC) were determined. Biofilm eradication was studied by crystal violet stain and light microscopy (LM), and confocal laser scanning microscopy (CLSM) was also utilized in conjunction with the image analysis software COMSTAT. Although all the OSBs studied had antifungalactivity, the cationic OSBs weremore effective than the anionic ones. A significant reduction of biofilms was observed at MIC and supraMIC50 (50 times higher than MIC) for compound 2, and at supraMIC50 with compound 3. Alterations in surface topography and the three-dimensional architecture of the biofilms were evident with LM and CLSM. The LM analysis revealed that the C. tropicalis strain produced a striking biofilmwith oval blastospores, pseudohyphae, and true hyphae. CLSM images showed that a decrease occurred in the thickness of the mature biofilms treated with the OSBs at the most effective concentration for each one. The results obtained by microscopy were supported by those of the COMSTAT program. Our results revealed an antibiofilm activity, with compound 2 being a potential candidate for the treatment of C. tropicalis infections.
Fil: Quinteros, Melisa de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Galera, Ivana Laura Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Tolosa, Juan Martín. Universidad de Castilla-La Mancha; España
Fil: García Martínez, Joaquín Calixto. Universidad de Castilla-La Mancha; España
Fil: Páez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
OLIGOSTYRYLBENZENES
CANDIDA TROPICALIS
BIOFILMS
PLANKTONIC CELLS
ANTIBIOFILM ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115398
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/115398
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilmsQuinteros, Melisa de Los ÁngelesGalera, Ivana Laura DeliaTolosa, Juan MartínGarcía Martínez, Joaquín CalixtoPáez, Paulina LauraParaje, María GabrielaOLIGOSTYRYLBENZENESCANDIDA TROPICALISBIOFILMSPLANKTONIC CELLSANTIBIOFILM ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1As sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene) vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. The minimum inhibitory concentration (MIC) for the planktonic population and the sessileminimum inhibitory concentrations (SMIC) were determined. Biofilm eradication was studied by crystal violet stain and light microscopy (LM), and confocal laser scanning microscopy (CLSM) was also utilized in conjunction with the image analysis software COMSTAT. Although all the OSBs studied had antifungalactivity, the cationic OSBs weremore effective than the anionic ones. A significant reduction of biofilms was observed at MIC and supraMIC50 (50 times higher than MIC) for compound 2, and at supraMIC50 with compound 3. Alterations in surface topography and the three-dimensional architecture of the biofilms were evident with LM and CLSM. The LM analysis revealed that the C. tropicalis strain produced a striking biofilmwith oval blastospores, pseudohyphae, and true hyphae. CLSM images showed that a decrease occurred in the thickness of the mature biofilms treated with the OSBs at the most effective concentration for each one. The results obtained by microscopy were supported by those of the COMSTAT program. Our results revealed an antibiofilm activity, with compound 2 being a potential candidate for the treatment of C. tropicalis infections.Fil: Quinteros, Melisa de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Galera, Ivana Laura Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Tolosa, Juan Martín. Universidad de Castilla-La Mancha; EspañaFil: García Martínez, Joaquín Calixto. Universidad de Castilla-La Mancha; EspañaFil: Páez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaTaylor & Francis Ltd2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115398Quinteros, Melisa de Los Ángeles; Galera, Ivana Laura Delia; Tolosa, Juan Martín; García Martínez, Joaquín Calixto; Páez, Paulina Laura; et al.; Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms; Taylor & Francis Ltd; Medical Mycology; 5-20201369-37861460-2709CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myaa046info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mmy/advance-article-abstract/doi/10.1093/mmy/myaa046/5857579info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:42Zoai:ri.conicet.gov.ar:11336/115398instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:42.667CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
title Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
spellingShingle Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
Quinteros, Melisa de Los Ángeles
OLIGOSTYRYLBENZENES
CANDIDA TROPICALIS
BIOFILMS
PLANKTONIC CELLS
ANTIBIOFILM ACTIVITY
title_short Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
title_full Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
title_fullStr Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
title_full_unstemmed Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
title_sort Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms
dc.creator.none.fl_str_mv Quinteros, Melisa de Los Ángeles
Galera, Ivana Laura Delia
Tolosa, Juan Martín
García Martínez, Joaquín Calixto
Páez, Paulina Laura
Paraje, María Gabriela
author Quinteros, Melisa de Los Ángeles
author_facet Quinteros, Melisa de Los Ángeles
Galera, Ivana Laura Delia
Tolosa, Juan Martín
García Martínez, Joaquín Calixto
Páez, Paulina Laura
Paraje, María Gabriela
author_role author
author2 Galera, Ivana Laura Delia
Tolosa, Juan Martín
García Martínez, Joaquín Calixto
Páez, Paulina Laura
Paraje, María Gabriela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv OLIGOSTYRYLBENZENES
CANDIDA TROPICALIS
BIOFILMS
PLANKTONIC CELLS
ANTIBIOFILM ACTIVITY
topic OLIGOSTYRYLBENZENES
CANDIDA TROPICALIS
BIOFILMS
PLANKTONIC CELLS
ANTIBIOFILM ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv As sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene) vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. The minimum inhibitory concentration (MIC) for the planktonic population and the sessileminimum inhibitory concentrations (SMIC) were determined. Biofilm eradication was studied by crystal violet stain and light microscopy (LM), and confocal laser scanning microscopy (CLSM) was also utilized in conjunction with the image analysis software COMSTAT. Although all the OSBs studied had antifungalactivity, the cationic OSBs weremore effective than the anionic ones. A significant reduction of biofilms was observed at MIC and supraMIC50 (50 times higher than MIC) for compound 2, and at supraMIC50 with compound 3. Alterations in surface topography and the three-dimensional architecture of the biofilms were evident with LM and CLSM. The LM analysis revealed that the C. tropicalis strain produced a striking biofilmwith oval blastospores, pseudohyphae, and true hyphae. CLSM images showed that a decrease occurred in the thickness of the mature biofilms treated with the OSBs at the most effective concentration for each one. The results obtained by microscopy were supported by those of the COMSTAT program. Our results revealed an antibiofilm activity, with compound 2 being a potential candidate for the treatment of C. tropicalis infections.
Fil: Quinteros, Melisa de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Galera, Ivana Laura Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Tolosa, Juan Martín. Universidad de Castilla-La Mancha; España
Fil: García Martínez, Joaquín Calixto. Universidad de Castilla-La Mancha; España
Fil: Páez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description As sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene) vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. The minimum inhibitory concentration (MIC) for the planktonic population and the sessileminimum inhibitory concentrations (SMIC) were determined. Biofilm eradication was studied by crystal violet stain and light microscopy (LM), and confocal laser scanning microscopy (CLSM) was also utilized in conjunction with the image analysis software COMSTAT. Although all the OSBs studied had antifungalactivity, the cationic OSBs weremore effective than the anionic ones. A significant reduction of biofilms was observed at MIC and supraMIC50 (50 times higher than MIC) for compound 2, and at supraMIC50 with compound 3. Alterations in surface topography and the three-dimensional architecture of the biofilms were evident with LM and CLSM. The LM analysis revealed that the C. tropicalis strain produced a striking biofilmwith oval blastospores, pseudohyphae, and true hyphae. CLSM images showed that a decrease occurred in the thickness of the mature biofilms treated with the OSBs at the most effective concentration for each one. The results obtained by microscopy were supported by those of the COMSTAT program. Our results revealed an antibiofilm activity, with compound 2 being a potential candidate for the treatment of C. tropicalis infections.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115398
Quinteros, Melisa de Los Ángeles; Galera, Ivana Laura Delia; Tolosa, Juan Martín; García Martínez, Joaquín Calixto; Páez, Paulina Laura; et al.; Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms; Taylor & Francis Ltd; Medical Mycology; 5-2020
1369-3786
1460-2709
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115398
identifier_str_mv Quinteros, Melisa de Los Ángeles; Galera, Ivana Laura Delia; Tolosa, Juan Martín; García Martínez, Joaquín Calixto; Páez, Paulina Laura; et al.; Novel antifungal activity of oligostyrylbenzenes compounds on Q5 Candida tropicalis biofilms; Taylor & Francis Ltd; Medical Mycology; 5-2020
1369-3786
1460-2709
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myaa046
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mmy/advance-article-abstract/doi/10.1093/mmy/myaa046/5857579
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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