Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)

Autores
Beamud, Sara Guadalupe; Karrasch, B.; Pedrozo, Fernando Luis; Diaz, Monica Mabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We investigated whether algal osmotrophy in naturally acidic Lake Caviahue is an important process for acquisition of organic carbon and organic nitrogen. To accomplish this, we quantified algal assimilation of organic compounds, measured the specific growth rate and biomass yield, and documented incorporation of organic compounds by phytoplankton in situ using microautoradiography. Substrate uptake quantification and microautoradiographic investigations were performed using 3 H-leucine, 3 H-glucose, 3 H-thymidine, 14C-aspartic acid, 14C-acetic acid and 14C-bicarbonates. The results showed that the most important species of the phytoplankton community, Keratococcus rhaphidioides and Watanabea sp., took up various sources of organic carbon and nitrogen under both light and dark conditions. They were also able to assimilate leucine, thymidine, aspartic acid and acetate under high levels of inorganic nitrogen and phosphorus, while they could use leucine, arginine, glutamine and glucose under low levels of nitrogen and phosphorus. The assimilation rates were higher in light than in darkness, and the algal specific growth rates increased when organic sources were added. We proposed that osmotrophy complements the main photosynthetic process of the phytoplankton in Lake Caviahue, which helps to overcome the scarcity of light and inorganic nitrogen and carbon in the water column.
Fil: Beamud, Sara Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Karrasch, B.. Helmholtz Centre For Environmental Research; Alemania
Fil: Pedrozo, Fernando Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Diaz, Monica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; Argentina
Materia
Osmotrophy
Acidic Lake
Keratococcus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12134
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)Beamud, Sara GuadalupeKarrasch, B.Pedrozo, Fernando LuisDiaz, Monica MabelOsmotrophyAcidic LakeKeratococcushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We investigated whether algal osmotrophy in naturally acidic Lake Caviahue is an important process for acquisition of organic carbon and organic nitrogen. To accomplish this, we quantified algal assimilation of organic compounds, measured the specific growth rate and biomass yield, and documented incorporation of organic compounds by phytoplankton in situ using microautoradiography. Substrate uptake quantification and microautoradiographic investigations were performed using 3 H-leucine, 3 H-glucose, 3 H-thymidine, 14C-aspartic acid, 14C-acetic acid and 14C-bicarbonates. The results showed that the most important species of the phytoplankton community, Keratococcus rhaphidioides and Watanabea sp., took up various sources of organic carbon and nitrogen under both light and dark conditions. They were also able to assimilate leucine, thymidine, aspartic acid and acetate under high levels of inorganic nitrogen and phosphorus, while they could use leucine, arginine, glutamine and glucose under low levels of nitrogen and phosphorus. The assimilation rates were higher in light than in darkness, and the algal specific growth rates increased when organic sources were added. We proposed that osmotrophy complements the main photosynthetic process of the phytoplankton in Lake Caviahue, which helps to overcome the scarcity of light and inorganic nitrogen and carbon in the water column.Fil: Beamud, Sara Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; ArgentinaFil: Karrasch, B.. Helmholtz Centre For Environmental Research; AlemaniaFil: Pedrozo, Fernando Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; ArgentinaFil: Diaz, Monica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; ArgentinaSpringer Tokyo2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12134Beamud, Sara Guadalupe; Karrasch, B.; Pedrozo, Fernando Luis; Diaz, Monica Mabel; Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina); Springer Tokyo; Limnology; 15; 2; 4-2014; 163–1721439-86211439-863Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10201-014-0427-2info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10201-014-0427-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:26Zoai:ri.conicet.gov.ar:11336/12134instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:27.26CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
title Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
spellingShingle Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
Beamud, Sara Guadalupe
Osmotrophy
Acidic Lake
Keratococcus
title_short Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
title_full Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
title_fullStr Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
title_full_unstemmed Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
title_sort Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina)
dc.creator.none.fl_str_mv Beamud, Sara Guadalupe
Karrasch, B.
Pedrozo, Fernando Luis
Diaz, Monica Mabel
author Beamud, Sara Guadalupe
author_facet Beamud, Sara Guadalupe
Karrasch, B.
Pedrozo, Fernando Luis
Diaz, Monica Mabel
author_role author
author2 Karrasch, B.
Pedrozo, Fernando Luis
Diaz, Monica Mabel
author2_role author
author
author
dc.subject.none.fl_str_mv Osmotrophy
Acidic Lake
Keratococcus
topic Osmotrophy
Acidic Lake
Keratococcus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We investigated whether algal osmotrophy in naturally acidic Lake Caviahue is an important process for acquisition of organic carbon and organic nitrogen. To accomplish this, we quantified algal assimilation of organic compounds, measured the specific growth rate and biomass yield, and documented incorporation of organic compounds by phytoplankton in situ using microautoradiography. Substrate uptake quantification and microautoradiographic investigations were performed using 3 H-leucine, 3 H-glucose, 3 H-thymidine, 14C-aspartic acid, 14C-acetic acid and 14C-bicarbonates. The results showed that the most important species of the phytoplankton community, Keratococcus rhaphidioides and Watanabea sp., took up various sources of organic carbon and nitrogen under both light and dark conditions. They were also able to assimilate leucine, thymidine, aspartic acid and acetate under high levels of inorganic nitrogen and phosphorus, while they could use leucine, arginine, glutamine and glucose under low levels of nitrogen and phosphorus. The assimilation rates were higher in light than in darkness, and the algal specific growth rates increased when organic sources were added. We proposed that osmotrophy complements the main photosynthetic process of the phytoplankton in Lake Caviahue, which helps to overcome the scarcity of light and inorganic nitrogen and carbon in the water column.
Fil: Beamud, Sara Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Karrasch, B.. Helmholtz Centre For Environmental Research; Alemania
Fil: Pedrozo, Fernando Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Diaz, Monica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue; Argentina
description We investigated whether algal osmotrophy in naturally acidic Lake Caviahue is an important process for acquisition of organic carbon and organic nitrogen. To accomplish this, we quantified algal assimilation of organic compounds, measured the specific growth rate and biomass yield, and documented incorporation of organic compounds by phytoplankton in situ using microautoradiography. Substrate uptake quantification and microautoradiographic investigations were performed using 3 H-leucine, 3 H-glucose, 3 H-thymidine, 14C-aspartic acid, 14C-acetic acid and 14C-bicarbonates. The results showed that the most important species of the phytoplankton community, Keratococcus rhaphidioides and Watanabea sp., took up various sources of organic carbon and nitrogen under both light and dark conditions. They were also able to assimilate leucine, thymidine, aspartic acid and acetate under high levels of inorganic nitrogen and phosphorus, while they could use leucine, arginine, glutamine and glucose under low levels of nitrogen and phosphorus. The assimilation rates were higher in light than in darkness, and the algal specific growth rates increased when organic sources were added. We proposed that osmotrophy complements the main photosynthetic process of the phytoplankton in Lake Caviahue, which helps to overcome the scarcity of light and inorganic nitrogen and carbon in the water column.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12134
Beamud, Sara Guadalupe; Karrasch, B.; Pedrozo, Fernando Luis; Diaz, Monica Mabel; Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina); Springer Tokyo; Limnology; 15; 2; 4-2014; 163–172
1439-8621
1439-863X
url http://hdl.handle.net/11336/12134
identifier_str_mv Beamud, Sara Guadalupe; Karrasch, B.; Pedrozo, Fernando Luis; Diaz, Monica Mabel; Utilisation of organic compounds by osmotrophic algae in an acidic lake of Patagonia (Argentina); Springer Tokyo; Limnology; 15; 2; 4-2014; 163–172
1439-8621
1439-863X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10201-014-0427-2
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10201-014-0427-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Tokyo
publisher.none.fl_str_mv Springer Tokyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270004243857408
score 13.13397

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