In vivo self-incompatibility response in the wild potato Solanum chacoense bitter

Autores
Capurro, Mauricio Antonio; Medina Piles, Vicente; Camadro, Elsa Lucila
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In tuber-bearing Solanum species (potatoes), S-locus mediated self-incompatibility is under gametophytic control. In this system, self-fertilization is avoided when the same S-allele is expressed in both pistil and pollen. Because the ultrastructural details of the self-incompatibility response in this group of species is unknown, the aim of this study was to identify the cellular events involved in this response by using the diploid species S. chacoense Bitter as a model. To this end, pollinations were carried out in two genotypic combinations previously identified as (1) compatible and (2) self-incompatible (self-pollination) for the S-locus. Pollinated pistils were fixed on an hourly basis and observed under a transmission electron microscope to detect ultrastructural changes. In the self-incompatible genotypic combination, pollen grains germinated normally but loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination (HAP), mitochondrial swelling started at 2 HAP and, finally, mitochondrial content was lost at 7 HAP, with the concomitant cessation of pollen tube growth in the upper third of the style. The gametophytic self-incompatibility response in potato shares similarities with a type of programmed cell death.
Fil: Capurro, Mauricio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Medina Piles, Vicente. Universidad de Lleida; España
Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Materia
Pollen tube mitochondria
Programmed cell death
Self-incompatibility
Tuber-bearing Solanum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270990
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo self-incompatibility response in the wild potato Solanum chacoense bitterCapurro, Mauricio AntonioMedina Piles, VicenteCamadro, Elsa LucilaPollen tube mitochondriaProgrammed cell deathSelf-incompatibilityTuber-bearing Solanumhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4In tuber-bearing Solanum species (potatoes), S-locus mediated self-incompatibility is under gametophytic control. In this system, self-fertilization is avoided when the same S-allele is expressed in both pistil and pollen. Because the ultrastructural details of the self-incompatibility response in this group of species is unknown, the aim of this study was to identify the cellular events involved in this response by using the diploid species S. chacoense Bitter as a model. To this end, pollinations were carried out in two genotypic combinations previously identified as (1) compatible and (2) self-incompatible (self-pollination) for the S-locus. Pollinated pistils were fixed on an hourly basis and observed under a transmission electron microscope to detect ultrastructural changes. In the self-incompatible genotypic combination, pollen grains germinated normally but loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination (HAP), mitochondrial swelling started at 2 HAP and, finally, mitochondrial content was lost at 7 HAP, with the concomitant cessation of pollen tube growth in the upper third of the style. The gametophytic self-incompatibility response in potato shares similarities with a type of programmed cell death.Fil: Capurro, Mauricio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Medina Piles, Vicente. Universidad de Lleida; EspañaFil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaSociedad Argentina de Genética2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270990Capurro, Mauricio Antonio; Medina Piles, Vicente; Camadro, Elsa Lucila; In vivo self-incompatibility response in the wild potato Solanum chacoense bitter; Sociedad Argentina de Genética; Basic and Applied Genetics; 23; 1; 6-2012; 42-461666-0390CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sag.org.ar/jbag/wp-content/uploads/2020/01/V.XXIII_2012_Issue1_06032013.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:11:06Zoai:ri.conicet.gov.ar:11336/270990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:06.817CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
title In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
spellingShingle In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
Capurro, Mauricio Antonio
Pollen tube mitochondria
Programmed cell death
Self-incompatibility
Tuber-bearing Solanum
title_short In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
title_full In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
title_fullStr In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
title_full_unstemmed In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
title_sort In vivo self-incompatibility response in the wild potato Solanum chacoense bitter
dc.creator.none.fl_str_mv Capurro, Mauricio Antonio
Medina Piles, Vicente
Camadro, Elsa Lucila
author Capurro, Mauricio Antonio
author_facet Capurro, Mauricio Antonio
Medina Piles, Vicente
Camadro, Elsa Lucila
author_role author
author2 Medina Piles, Vicente
Camadro, Elsa Lucila
author2_role author
author
dc.subject.none.fl_str_mv Pollen tube mitochondria
Programmed cell death
Self-incompatibility
Tuber-bearing Solanum
topic Pollen tube mitochondria
Programmed cell death
Self-incompatibility
Tuber-bearing Solanum
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In tuber-bearing Solanum species (potatoes), S-locus mediated self-incompatibility is under gametophytic control. In this system, self-fertilization is avoided when the same S-allele is expressed in both pistil and pollen. Because the ultrastructural details of the self-incompatibility response in this group of species is unknown, the aim of this study was to identify the cellular events involved in this response by using the diploid species S. chacoense Bitter as a model. To this end, pollinations were carried out in two genotypic combinations previously identified as (1) compatible and (2) self-incompatible (self-pollination) for the S-locus. Pollinated pistils were fixed on an hourly basis and observed under a transmission electron microscope to detect ultrastructural changes. In the self-incompatible genotypic combination, pollen grains germinated normally but loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination (HAP), mitochondrial swelling started at 2 HAP and, finally, mitochondrial content was lost at 7 HAP, with the concomitant cessation of pollen tube growth in the upper third of the style. The gametophytic self-incompatibility response in potato shares similarities with a type of programmed cell death.
Fil: Capurro, Mauricio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Medina Piles, Vicente. Universidad de Lleida; España
Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
description In tuber-bearing Solanum species (potatoes), S-locus mediated self-incompatibility is under gametophytic control. In this system, self-fertilization is avoided when the same S-allele is expressed in both pistil and pollen. Because the ultrastructural details of the self-incompatibility response in this group of species is unknown, the aim of this study was to identify the cellular events involved in this response by using the diploid species S. chacoense Bitter as a model. To this end, pollinations were carried out in two genotypic combinations previously identified as (1) compatible and (2) self-incompatible (self-pollination) for the S-locus. Pollinated pistils were fixed on an hourly basis and observed under a transmission electron microscope to detect ultrastructural changes. In the self-incompatible genotypic combination, pollen grains germinated normally but loss of electron density in the mitochondrial matrix of pollen tubes was observed at 1 hour after pollination (HAP), mitochondrial swelling started at 2 HAP and, finally, mitochondrial content was lost at 7 HAP, with the concomitant cessation of pollen tube growth in the upper third of the style. The gametophytic self-incompatibility response in potato shares similarities with a type of programmed cell death.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270990
Capurro, Mauricio Antonio; Medina Piles, Vicente; Camadro, Elsa Lucila; In vivo self-incompatibility response in the wild potato Solanum chacoense bitter; Sociedad Argentina de Genética; Basic and Applied Genetics; 23; 1; 6-2012; 42-46
1666-0390
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270990
identifier_str_mv Capurro, Mauricio Antonio; Medina Piles, Vicente; Camadro, Elsa Lucila; In vivo self-incompatibility response in the wild potato Solanum chacoense bitter; Sociedad Argentina de Genética; Basic and Applied Genetics; 23; 1; 6-2012; 42-46
1666-0390
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sag.org.ar/jbag/wp-content/uploads/2020/01/V.XXIII_2012_Issue1_06032013.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Genética
publisher.none.fl_str_mv Sociedad Argentina de Genética
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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