A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula

Autores
Pasten, Maria Cielo; Carballo, José; Gallardo, Jimena Alicia; Zappacosta, Diego Carlos; Selva, Juan Pablo; Rodrigo, Juan Manuel; Echenique, Carmen Viviana; Garbus, Ingrid
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass typicallyestablished in semi-arid regions, with good adaptability to dry conditions andsandy soils. This polymorphic complex includes both sexual and apomicticcytotypes, with different ploidy levels (2x-8x). Diploids are known to be sexual,while most polyploids are facultative apomicts, and full apomicts have also beenreported. Plant breeding studies throughout the years have focused on achievingthe introgression of apomixis into species of agricultural relevance, but, given thecomplexity of the trait, a deeper understanding of themolecular basis of regulatorymechanisms of apomixis is still required. Apomixis is thought to be associated withsilencing or disruption of the sexual pathway, and studies have shown it isinfluenced by epigenetic mechanisms. In a previous study, we explored the roleof miRNA-mRNA interactions using two contrasting E. curvula phenotypes. Here,the sexual OTA-S, the facultative Don Walter and the obligate apomicticTanganyika cDNA and sRNA libraries were inquired, searching for miRNAdiscovery and miRNA expression regulation of genes related to the reproductivemode. This allowed for the characterization of seven miRNAs and the validation oftheir miRNA-target interactions. Interestingly, a kinesin gene was found to berepressed in the apomictic cultivar Tanganyika, targeted by a novelmiRNA thatwasfound to be overexpressed in this genotype, suggestive of an involvement in thereproductive mode expression. Our work provided additional evidence of thecontribution of the epigenetic regulation of the apomictic pathway.
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
APOMIXIS
ERAGROSTIS CURVULA
MIRNA
MIRNA-MRNA INTERACTION
SRNA LIBRARIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197038
Acceder
id CONICETDig_96b59b8b8f0c30b155f0fb17ee07afe9
oai_identifier_str oai:ri.conicet.gov.ar:11336/197038
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvulaPasten, Maria CieloCarballo, JoséGallardo, Jimena AliciaZappacosta, Diego CarlosSelva, Juan PabloRodrigo, Juan ManuelEchenique, Carmen VivianaGarbus, IngridAPOMIXISERAGROSTIS CURVULAMIRNAMIRNA-MRNA INTERACTIONSRNA LIBRARIEShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass typicallyestablished in semi-arid regions, with good adaptability to dry conditions andsandy soils. This polymorphic complex includes both sexual and apomicticcytotypes, with different ploidy levels (2x-8x). Diploids are known to be sexual,while most polyploids are facultative apomicts, and full apomicts have also beenreported. Plant breeding studies throughout the years have focused on achievingthe introgression of apomixis into species of agricultural relevance, but, given thecomplexity of the trait, a deeper understanding of themolecular basis of regulatorymechanisms of apomixis is still required. Apomixis is thought to be associated withsilencing or disruption of the sexual pathway, and studies have shown it isinfluenced by epigenetic mechanisms. In a previous study, we explored the roleof miRNA-mRNA interactions using two contrasting E. curvula phenotypes. Here,the sexual OTA-S, the facultative Don Walter and the obligate apomicticTanganyika cDNA and sRNA libraries were inquired, searching for miRNAdiscovery and miRNA expression regulation of genes related to the reproductivemode. This allowed for the characterization of seven miRNAs and the validation oftheir miRNA-target interactions. Interestingly, a kinesin gene was found to berepressed in the apomictic cultivar Tanganyika, targeted by a novelmiRNA thatwasfound to be overexpressed in this genotype, suggestive of an involvement in thereproductive mode expression. Our work provided additional evidence of thecontribution of the epigenetic regulation of the apomictic pathway.Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFrontiers Media S.A.2022-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197038Pasten, Maria Cielo; Carballo, José; Gallardo, Jimena Alicia; Zappacosta, Diego Carlos; Selva, Juan Pablo; et al.; A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula; Frontiers Media S.A.; Frontiers in Plant Science; 13; 30-9-2022; 1-171664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2022.1012682/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2022.1012682info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:51:31Zoai:ri.conicet.gov.ar:11336/197038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:51:31.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
title A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
spellingShingle A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
Pasten, Maria Cielo
APOMIXIS
ERAGROSTIS CURVULA
MIRNA
MIRNA-MRNA INTERACTION
SRNA LIBRARIES
title_short A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
title_full A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
title_fullStr A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
title_full_unstemmed A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
title_sort A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
dc.creator.none.fl_str_mv Pasten, Maria Cielo
Carballo, José
Gallardo, Jimena Alicia
Zappacosta, Diego Carlos
Selva, Juan Pablo
Rodrigo, Juan Manuel
Echenique, Carmen Viviana
Garbus, Ingrid
author Pasten, Maria Cielo
author_facet Pasten, Maria Cielo
Carballo, José
Gallardo, Jimena Alicia
Zappacosta, Diego Carlos
Selva, Juan Pablo
Rodrigo, Juan Manuel
Echenique, Carmen Viviana
Garbus, Ingrid
author_role author
author2 Carballo, José
Gallardo, Jimena Alicia
Zappacosta, Diego Carlos
Selva, Juan Pablo
Rodrigo, Juan Manuel
Echenique, Carmen Viviana
Garbus, Ingrid
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOMIXIS
ERAGROSTIS CURVULA
MIRNA
MIRNA-MRNA INTERACTION
SRNA LIBRARIES
topic APOMIXIS
ERAGROSTIS CURVULA
MIRNA
MIRNA-MRNA INTERACTION
SRNA LIBRARIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass typicallyestablished in semi-arid regions, with good adaptability to dry conditions andsandy soils. This polymorphic complex includes both sexual and apomicticcytotypes, with different ploidy levels (2x-8x). Diploids are known to be sexual,while most polyploids are facultative apomicts, and full apomicts have also beenreported. Plant breeding studies throughout the years have focused on achievingthe introgression of apomixis into species of agricultural relevance, but, given thecomplexity of the trait, a deeper understanding of themolecular basis of regulatorymechanisms of apomixis is still required. Apomixis is thought to be associated withsilencing or disruption of the sexual pathway, and studies have shown it isinfluenced by epigenetic mechanisms. In a previous study, we explored the roleof miRNA-mRNA interactions using two contrasting E. curvula phenotypes. Here,the sexual OTA-S, the facultative Don Walter and the obligate apomicticTanganyika cDNA and sRNA libraries were inquired, searching for miRNAdiscovery and miRNA expression regulation of genes related to the reproductivemode. This allowed for the characterization of seven miRNAs and the validation oftheir miRNA-target interactions. Interestingly, a kinesin gene was found to berepressed in the apomictic cultivar Tanganyika, targeted by a novelmiRNA thatwasfound to be overexpressed in this genotype, suggestive of an involvement in thereproductive mode expression. Our work provided additional evidence of thecontribution of the epigenetic regulation of the apomictic pathway.
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass typicallyestablished in semi-arid regions, with good adaptability to dry conditions andsandy soils. This polymorphic complex includes both sexual and apomicticcytotypes, with different ploidy levels (2x-8x). Diploids are known to be sexual,while most polyploids are facultative apomicts, and full apomicts have also beenreported. Plant breeding studies throughout the years have focused on achievingthe introgression of apomixis into species of agricultural relevance, but, given thecomplexity of the trait, a deeper understanding of themolecular basis of regulatorymechanisms of apomixis is still required. Apomixis is thought to be associated withsilencing or disruption of the sexual pathway, and studies have shown it isinfluenced by epigenetic mechanisms. In a previous study, we explored the roleof miRNA-mRNA interactions using two contrasting E. curvula phenotypes. Here,the sexual OTA-S, the facultative Don Walter and the obligate apomicticTanganyika cDNA and sRNA libraries were inquired, searching for miRNAdiscovery and miRNA expression regulation of genes related to the reproductivemode. This allowed for the characterization of seven miRNAs and the validation oftheir miRNA-target interactions. Interestingly, a kinesin gene was found to berepressed in the apomictic cultivar Tanganyika, targeted by a novelmiRNA thatwasfound to be overexpressed in this genotype, suggestive of an involvement in thereproductive mode expression. Our work provided additional evidence of thecontribution of the epigenetic regulation of the apomictic pathway.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197038
Pasten, Maria Cielo; Carballo, José; Gallardo, Jimena Alicia; Zappacosta, Diego Carlos; Selva, Juan Pablo; et al.; A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula; Frontiers Media S.A.; Frontiers in Plant Science; 13; 30-9-2022; 1-17
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197038
identifier_str_mv Pasten, Maria Cielo; Carballo, José; Gallardo, Jimena Alicia; Zappacosta, Diego Carlos; Selva, Juan Pablo; et al.; A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula; Frontiers Media S.A.; Frontiers in Plant Science; 13; 30-9-2022; 1-17
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2022.1012682/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2022.1012682
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083041333608448
score 12.891075

Publicaciones similares