MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene

Autores
Giovambattista, Guillermo; Diaz, Silvina; Ripoli, María Verónica; Peral Garcia, Pilar
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
At present, 53 PCR-RFLP alleles of the bovine DRB3 exon 2 were reported. In order to analyse the putative MspI restriction patterns present in the exon 2 of BoLA-DRB3 gene, all available DNA sequences were retrieved from Genbank Data Library. In this analysis, two MspI restriction sites and five restriction patterns were found. In addition, the observed restriction patterns obtained after MspI digestion of genomic DNA were compared with those predicted from the DRB3 DNA sequence analysis. The use of MspI restriction enzymes together with BstYI, HaeIII and RsaI could be useful in recognising some sequences differences in a single PCR-RFLP allele as defined by the previously used combination of enzyme, improving the robustness of this typing method designed by van Eijk et al. (1992). Despite the number of restriction patterns detected by MspI and BstYI are similar, the MspI patterns are more easily identified than those of BstYI. MspI enzyme works well in the same reaction buffer and temperature as HaeIII and RsaI, thus simplifying double digestions. Finally, the costs may be reduced.
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Diaz, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
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acceso abierto
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https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/83778
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network_name_str CONICET Digital (CONICET)
spelling MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 geneGiovambattista, GuillermoDiaz, SilvinaRipoli, María VerónicaPeral Garcia, Pilarhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4At present, 53 PCR-RFLP alleles of the bovine DRB3 exon 2 were reported. In order to analyse the putative MspI restriction patterns present in the exon 2 of BoLA-DRB3 gene, all available DNA sequences were retrieved from Genbank Data Library. In this analysis, two MspI restriction sites and five restriction patterns were found. In addition, the observed restriction patterns obtained after MspI digestion of genomic DNA were compared with those predicted from the DRB3 DNA sequence analysis. The use of MspI restriction enzymes together with BstYI, HaeIII and RsaI could be useful in recognising some sequences differences in a single PCR-RFLP allele as defined by the previously used combination of enzyme, improving the robustness of this typing method designed by van Eijk et al. (1992). Despite the number of restriction patterns detected by MspI and BstYI are similar, the MspI patterns are more easily identified than those of BstYI. MspI enzyme works well in the same reaction buffer and temperature as HaeIII and RsaI, thus simplifying double digestions. Finally, the costs may be reduced.Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; ArgentinaFil: Diaz, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; ArgentinaFil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; ArgentinaFil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; ArgentinaSociedad Argentina de Genética2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/83778Giovambattista, Guillermo; Diaz, Silvina; Ripoli, María Verónica; Peral Garcia, Pilar; MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene; Sociedad Argentina de Genética; BAG Journal of Basic Applied Genetics; 15; 2; 12-2003; 63-671852-62331666-0390CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sag.org.ar/jbag/contenidos/info:eu-repo/semantics/altIdentifier/url/http://www.scielo.org.ar/scielo.php?script=sci_serial&pid=1852-6233&lng=es&nrm=isoinfo:eu-repo/semantics/altIdentifier/url/http://ppct.caicyt.gov.ar/index.php/bag/indexinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:03Zoai:ri.conicet.gov.ar:11336/83778instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:03.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
title MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
spellingShingle MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
Giovambattista, Guillermo
title_short MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
title_full MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
title_fullStr MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
title_full_unstemmed MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
title_sort MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene
dc.creator.none.fl_str_mv Giovambattista, Guillermo
Diaz, Silvina
Ripoli, María Verónica
Peral Garcia, Pilar
author Giovambattista, Guillermo
author_facet Giovambattista, Guillermo
Diaz, Silvina
Ripoli, María Verónica
Peral Garcia, Pilar
author_role author
author2 Diaz, Silvina
Ripoli, María Verónica
Peral Garcia, Pilar
author2_role author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv At present, 53 PCR-RFLP alleles of the bovine DRB3 exon 2 were reported. In order to analyse the putative MspI restriction patterns present in the exon 2 of BoLA-DRB3 gene, all available DNA sequences were retrieved from Genbank Data Library. In this analysis, two MspI restriction sites and five restriction patterns were found. In addition, the observed restriction patterns obtained after MspI digestion of genomic DNA were compared with those predicted from the DRB3 DNA sequence analysis. The use of MspI restriction enzymes together with BstYI, HaeIII and RsaI could be useful in recognising some sequences differences in a single PCR-RFLP allele as defined by the previously used combination of enzyme, improving the robustness of this typing method designed by van Eijk et al. (1992). Despite the number of restriction patterns detected by MspI and BstYI are similar, the MspI patterns are more easily identified than those of BstYI. MspI enzyme works well in the same reaction buffer and temperature as HaeIII and RsaI, thus simplifying double digestions. Finally, the costs may be reduced.
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Diaz, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
description At present, 53 PCR-RFLP alleles of the bovine DRB3 exon 2 were reported. In order to analyse the putative MspI restriction patterns present in the exon 2 of BoLA-DRB3 gene, all available DNA sequences were retrieved from Genbank Data Library. In this analysis, two MspI restriction sites and five restriction patterns were found. In addition, the observed restriction patterns obtained after MspI digestion of genomic DNA were compared with those predicted from the DRB3 DNA sequence analysis. The use of MspI restriction enzymes together with BstYI, HaeIII and RsaI could be useful in recognising some sequences differences in a single PCR-RFLP allele as defined by the previously used combination of enzyme, improving the robustness of this typing method designed by van Eijk et al. (1992). Despite the number of restriction patterns detected by MspI and BstYI are similar, the MspI patterns are more easily identified than those of BstYI. MspI enzyme works well in the same reaction buffer and temperature as HaeIII and RsaI, thus simplifying double digestions. Finally, the costs may be reduced.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/83778
Giovambattista, Guillermo; Diaz, Silvina; Ripoli, María Verónica; Peral Garcia, Pilar; MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene; Sociedad Argentina de Genética; BAG Journal of Basic Applied Genetics; 15; 2; 12-2003; 63-67
1852-6233
1666-0390
CONICET Digital
CONICET
url http://hdl.handle.net/11336/83778
identifier_str_mv Giovambattista, Guillermo; Diaz, Silvina; Ripoli, María Verónica; Peral Garcia, Pilar; MspI restriction enzyme evidence moderate polymorphism in the second exon of BoLA-DRB3 gene; Sociedad Argentina de Genética; BAG Journal of Basic Applied Genetics; 15; 2; 12-2003; 63-67
1852-6233
1666-0390
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/http://ppct.caicyt.gov.ar/index.php/bag/index
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Genética
publisher.none.fl_str_mv Sociedad Argentina de Genética
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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