Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle

Autores
Goszczynski, Daniel Estanislao; Ripoli, María Verónica; Takeshima, S.N.; Baltian, Laura Rosana; Aida, Y.; Giovambattista, Guillermo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polymorphisms of the BoLA-DRB3 gene are located primarily in the second exon [antigen binding site (ABS)] and, to a lesser extent, in the upstream regulatory region (URR). It can be hypothesised that exon 2 and the URR are under different types of natural selection. The aim of this work was to determine the URR-exon 2 haplotypes; 34 Holstein samples were genotyped by direct sequencing. A total of 7 URR alleles and 23 exon 2 alleles were detected, and 3 of the URR alleles were novel. Our results may suggest that no relationship exists between the URR and exon 2 of the BoLA-DRB3 gene (linkage disequilibrium P value > 0.05), most likely due to recombination over time. Our results also suggest that both regions of class II genes may be included in the development of new genotyping methods based on nextgeneration DNA sequencing technologies.
Fil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Takeshima, S.N.. Viral Infectious Diseases Unit; Japón
Fil: Baltian, Laura Rosana. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aida, Y.. Viral Infectious Diseases Unit; Japón
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
Bola-Drb3
Linkage Disequilibrium
Polymorphism
Selection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24845
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24845
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattleGoszczynski, Daniel EstanislaoRipoli, María VerónicaTakeshima, S.N.Baltian, Laura RosanaAida, Y.Giovambattista, GuillermoBola-Drb3Linkage DisequilibriumPolymorphismSelectionPolymorphisms of the BoLA-DRB3 gene are located primarily in the second exon [antigen binding site (ABS)] and, to a lesser extent, in the upstream regulatory region (URR). It can be hypothesised that exon 2 and the URR are under different types of natural selection. The aim of this work was to determine the URR-exon 2 haplotypes; 34 Holstein samples were genotyped by direct sequencing. A total of 7 URR alleles and 23 exon 2 alleles were detected, and 3 of the URR alleles were novel. Our results may suggest that no relationship exists between the URR and exon 2 of the BoLA-DRB3 gene (linkage disequilibrium P value > 0.05), most likely due to recombination over time. Our results also suggest that both regions of class II genes may be included in the development of new genotyping methods based on nextgeneration DNA sequencing technologies.Fil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Takeshima, S.N.. Viral Infectious Diseases Unit; JapónFil: Baltian, Laura Rosana. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aida, Y.. Viral Infectious Diseases Unit; JapónFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaWiley Blackwell Publishing, Inc2014-02-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24845Goszczynski, Daniel Estanislao; Ripoli, María Verónica; Takeshima, S.N.; Baltian, Laura Rosana; Aida, Y.; et al.; Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle; Wiley Blackwell Publishing, Inc; Tissue Antigens; 83; 3; 6-2-2014; 180-1830001-2815CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/tan.12293info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tan.12293/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:42Zoai:ri.conicet.gov.ar:11336/24845instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:43.264CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
title Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
spellingShingle Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
Goszczynski, Daniel Estanislao
Bola-Drb3
Linkage Disequilibrium
Polymorphism
Selection
title_short Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
title_full Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
title_fullStr Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
title_full_unstemmed Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
title_sort Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle
dc.creator.none.fl_str_mv Goszczynski, Daniel Estanislao
Ripoli, María Verónica
Takeshima, S.N.
Baltian, Laura Rosana
Aida, Y.
Giovambattista, Guillermo
author Goszczynski, Daniel Estanislao
author_facet Goszczynski, Daniel Estanislao
Ripoli, María Verónica
Takeshima, S.N.
Baltian, Laura Rosana
Aida, Y.
Giovambattista, Guillermo
author_role author
author2 Ripoli, María Verónica
Takeshima, S.N.
Baltian, Laura Rosana
Aida, Y.
Giovambattista, Guillermo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bola-Drb3
Linkage Disequilibrium
Polymorphism
Selection
topic Bola-Drb3
Linkage Disequilibrium
Polymorphism
Selection
dc.description.none.fl_txt_mv Polymorphisms of the BoLA-DRB3 gene are located primarily in the second exon [antigen binding site (ABS)] and, to a lesser extent, in the upstream regulatory region (URR). It can be hypothesised that exon 2 and the URR are under different types of natural selection. The aim of this work was to determine the URR-exon 2 haplotypes; 34 Holstein samples were genotyped by direct sequencing. A total of 7 URR alleles and 23 exon 2 alleles were detected, and 3 of the URR alleles were novel. Our results may suggest that no relationship exists between the URR and exon 2 of the BoLA-DRB3 gene (linkage disequilibrium P value > 0.05), most likely due to recombination over time. Our results also suggest that both regions of class II genes may be included in the development of new genotyping methods based on nextgeneration DNA sequencing technologies.
Fil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Takeshima, S.N.. Viral Infectious Diseases Unit; Japón
Fil: Baltian, Laura Rosana. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aida, Y.. Viral Infectious Diseases Unit; Japón
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Polymorphisms of the BoLA-DRB3 gene are located primarily in the second exon [antigen binding site (ABS)] and, to a lesser extent, in the upstream regulatory region (URR). It can be hypothesised that exon 2 and the URR are under different types of natural selection. The aim of this work was to determine the URR-exon 2 haplotypes; 34 Holstein samples were genotyped by direct sequencing. A total of 7 URR alleles and 23 exon 2 alleles were detected, and 3 of the URR alleles were novel. Our results may suggest that no relationship exists between the URR and exon 2 of the BoLA-DRB3 gene (linkage disequilibrium P value > 0.05), most likely due to recombination over time. Our results also suggest that both regions of class II genes may be included in the development of new genotyping methods based on nextgeneration DNA sequencing technologies.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24845
Goszczynski, Daniel Estanislao; Ripoli, María Verónica; Takeshima, S.N.; Baltian, Laura Rosana; Aida, Y.; et al.; Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle; Wiley Blackwell Publishing, Inc; Tissue Antigens; 83; 3; 6-2-2014; 180-183
0001-2815
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24845
identifier_str_mv Goszczynski, Daniel Estanislao; Ripoli, María Verónica; Takeshima, S.N.; Baltian, Laura Rosana; Aida, Y.; et al.; Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle; Wiley Blackwell Publishing, Inc; Tissue Antigens; 83; 3; 6-2-2014; 180-183
0001-2815
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/tan.12293
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tan.12293/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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