MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro

Autores
Reynoso, Rita Paola; Laufer, Natalia Lorna; Hackl, Matthias; Skalicky, Susanna; Monteforte, Rossella; Turk, Gabriela Julia Ana; Carobene, Mauricio; Quarleri, Jorge Fabian; Cahn, Pedro; Werner, Roland; Stoiber, Heribert; Grillari Voglauer, Regina; Grillari, Johannes
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several miRNAs correlate with CD41 T cell count or with the known time of infection. No significant differences were observed between elite controllers and healthy donors; however, 16 miRNAs were different in the plasma of chronic infected versus healthy donors. In addition, levels of hsa-miR-29b-3p, hsa-miR-33a-5p and hsa-miR-146a-5p were higher in plasma from elite controllers than chronic infected and hsa-miR-29b-3p and hsa-miR-33a-5p overexpression significantly reduced the viral production in MT2 and primary T CD41 cells. Therefore, levels of circulating miRNAs might be of diagnostic and/or prognostic value for HIV infection, and hsa-miR-29b-3p and miR-33a-5p may contribute to the design of new anti-HIV drugs.
Fil: Reynoso, Rita Paola. University of Natural Resources and Life Sciences; Austria
Fil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina. Universidad de Buenos Aires; Argentina
Fil: Hackl, Matthias. University of Natural Resources and Life Sciences; Austria. Tamirna Gmbh; Austria
Fil: Skalicky, Susanna. Evercyte Gmbh; Austria
Fil: Monteforte, Rossella. University of Natural Resources and Life Sciences; Austria
Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires; Argentina
Fil: Carobene, Mauricio. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Quarleri, Jorge Fabian. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Cahn, Pedro. Fundación Huésped; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina
Fil: Werner, Roland. Medizinische Universitat Innsbruck; Austria
Fil: Stoiber, Heribert. Medizinische Univesitat Innsbruck; Austria
Fil: Grillari Voglauer, Regina. University of Natural Resources and Life Sciences; Austria. Evercyte Gmbh; Austria
Fil: Grillari, Johannes. University of Natural Resources and Life Sciences; Austria. Evercyte Gmbh; Austria
Materia
HIV
ELITE CONTROLLERS
MICRORNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113155
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113155
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitroReynoso, Rita PaolaLaufer, Natalia LornaHackl, MatthiasSkalicky, SusannaMonteforte, RossellaTurk, Gabriela Julia AnaCarobene, MauricioQuarleri, Jorge FabianCahn, PedroWerner, RolandStoiber, HeribertGrillari Voglauer, ReginaGrillari, JohannesHIVELITE CONTROLLERSMICRORNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several miRNAs correlate with CD41 T cell count or with the known time of infection. No significant differences were observed between elite controllers and healthy donors; however, 16 miRNAs were different in the plasma of chronic infected versus healthy donors. In addition, levels of hsa-miR-29b-3p, hsa-miR-33a-5p and hsa-miR-146a-5p were higher in plasma from elite controllers than chronic infected and hsa-miR-29b-3p and hsa-miR-33a-5p overexpression significantly reduced the viral production in MT2 and primary T CD41 cells. Therefore, levels of circulating miRNAs might be of diagnostic and/or prognostic value for HIV infection, and hsa-miR-29b-3p and miR-33a-5p may contribute to the design of new anti-HIV drugs.Fil: Reynoso, Rita Paola. University of Natural Resources and Life Sciences; AustriaFil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina. Universidad de Buenos Aires; ArgentinaFil: Hackl, Matthias. University of Natural Resources and Life Sciences; Austria. Tamirna Gmbh; AustriaFil: Skalicky, Susanna. Evercyte Gmbh; AustriaFil: Monteforte, Rossella. University of Natural Resources and Life Sciences; AustriaFil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires; ArgentinaFil: Carobene, Mauricio. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Quarleri, Jorge Fabian. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Cahn, Pedro. Fundación Huésped; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Werner, Roland. Medizinische Universitat Innsbruck; AustriaFil: Stoiber, Heribert. Medizinische Univesitat Innsbruck; AustriaFil: Grillari Voglauer, Regina. University of Natural Resources and Life Sciences; Austria. Evercyte Gmbh; AustriaFil: Grillari, Johannes. University of Natural Resources and Life Sciences; Austria. Evercyte Gmbh; AustriaNature Publishing Group2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113155Reynoso, Rita Paola; Laufer, Natalia Lorna; Hackl, Matthias; Skalicky, Susanna; Monteforte, Rossella; et al.; MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro; Nature Publishing Group; Scientific Reports; 4; 5915; 8-2014; 1-92045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/srep/2014/140801/srep05915/full/srep05915.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep05915info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:04Zoai:ri.conicet.gov.ar:11336/113155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:05.059CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
title MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
spellingShingle MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
Reynoso, Rita Paola
HIV
ELITE CONTROLLERS
MICRORNA
title_short MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
title_full MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
title_fullStr MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
title_full_unstemmed MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
title_sort MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro
dc.creator.none.fl_str_mv Reynoso, Rita Paola
Laufer, Natalia Lorna
Hackl, Matthias
Skalicky, Susanna
Monteforte, Rossella
Turk, Gabriela Julia Ana
Carobene, Mauricio
Quarleri, Jorge Fabian
Cahn, Pedro
Werner, Roland
Stoiber, Heribert
Grillari Voglauer, Regina
Grillari, Johannes
author Reynoso, Rita Paola
author_facet Reynoso, Rita Paola
Laufer, Natalia Lorna
Hackl, Matthias
Skalicky, Susanna
Monteforte, Rossella
Turk, Gabriela Julia Ana
Carobene, Mauricio
Quarleri, Jorge Fabian
Cahn, Pedro
Werner, Roland
Stoiber, Heribert
Grillari Voglauer, Regina
Grillari, Johannes
author_role author
author2 Laufer, Natalia Lorna
Hackl, Matthias
Skalicky, Susanna
Monteforte, Rossella
Turk, Gabriela Julia Ana
Carobene, Mauricio
Quarleri, Jorge Fabian
Cahn, Pedro
Werner, Roland
Stoiber, Heribert
Grillari Voglauer, Regina
Grillari, Johannes
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HIV
ELITE CONTROLLERS
MICRORNA
topic HIV
ELITE CONTROLLERS
MICRORNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several miRNAs correlate with CD41 T cell count or with the known time of infection. No significant differences were observed between elite controllers and healthy donors; however, 16 miRNAs were different in the plasma of chronic infected versus healthy donors. In addition, levels of hsa-miR-29b-3p, hsa-miR-33a-5p and hsa-miR-146a-5p were higher in plasma from elite controllers than chronic infected and hsa-miR-29b-3p and hsa-miR-33a-5p overexpression significantly reduced the viral production in MT2 and primary T CD41 cells. Therefore, levels of circulating miRNAs might be of diagnostic and/or prognostic value for HIV infection, and hsa-miR-29b-3p and miR-33a-5p may contribute to the design of new anti-HIV drugs.
Fil: Reynoso, Rita Paola. University of Natural Resources and Life Sciences; Austria
Fil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina. Universidad de Buenos Aires; Argentina
Fil: Hackl, Matthias. University of Natural Resources and Life Sciences; Austria. Tamirna Gmbh; Austria
Fil: Skalicky, Susanna. Evercyte Gmbh; Austria
Fil: Monteforte, Rossella. University of Natural Resources and Life Sciences; Austria
Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires; Argentina
Fil: Carobene, Mauricio. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Quarleri, Jorge Fabian. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Cahn, Pedro. Fundación Huésped; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina
Fil: Werner, Roland. Medizinische Universitat Innsbruck; Austria
Fil: Stoiber, Heribert. Medizinische Univesitat Innsbruck; Austria
Fil: Grillari Voglauer, Regina. University of Natural Resources and Life Sciences; Austria. Evercyte Gmbh; Austria
Fil: Grillari, Johannes. University of Natural Resources and Life Sciences; Austria. Evercyte Gmbh; Austria
description Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several miRNAs correlate with CD41 T cell count or with the known time of infection. No significant differences were observed between elite controllers and healthy donors; however, 16 miRNAs were different in the plasma of chronic infected versus healthy donors. In addition, levels of hsa-miR-29b-3p, hsa-miR-33a-5p and hsa-miR-146a-5p were higher in plasma from elite controllers than chronic infected and hsa-miR-29b-3p and hsa-miR-33a-5p overexpression significantly reduced the viral production in MT2 and primary T CD41 cells. Therefore, levels of circulating miRNAs might be of diagnostic and/or prognostic value for HIV infection, and hsa-miR-29b-3p and miR-33a-5p may contribute to the design of new anti-HIV drugs.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113155
Reynoso, Rita Paola; Laufer, Natalia Lorna; Hackl, Matthias; Skalicky, Susanna; Monteforte, Rossella; et al.; MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro; Nature Publishing Group; Scientific Reports; 4; 5915; 8-2014; 1-9
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113155
identifier_str_mv Reynoso, Rita Paola; Laufer, Natalia Lorna; Hackl, Matthias; Skalicky, Susanna; Monteforte, Rossella; et al.; MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro; Nature Publishing Group; Scientific Reports; 4; 5915; 8-2014; 1-9
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/srep/2014/140801/srep05915/full/srep05915.html
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep05915
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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