Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to c...

Autores
Brodeur, Celine Marie Julie; Svartz, Gabriela Veronica; Perez Coll, Cristina Silvia; Marino, Damian Jose Gabriel; Herkovits, Jorge
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acute and subchronic toxicity of atrazine was evaluated in embryos (stage 4) and in premetamorphosis (stage 25) and prometamorphosis (stage 38¡V39) larvae of the common South American toad Rhinella arenarum (Anura: bufonidae). The influence of atrazine on the last stages of metamorphosis was also examined by exposing prometamorphosis larvae until completion of metamorphosis. Results obtained revealed that larvae in premetamorphosis are more sensitive than larvae in prometamorphosis and that these are, in turn, more sensitive than embryonic stages. Indeed, concentrations of atrazine as high as 30 mg/L had little effects on embryonic stages, the embryos surviving and developing in a similar manner as controls. LC50s of premetamorphosis larvae equaled 27.16, 7.03 and 2.32 mg/L of atrazine after 4, 14 and 21 days of exposure, respectively, compared to LC50s values of 18.27 and 14.43 mg/L after 14 and 21 days of exposure for larvae in prometamorphosis. In experiments with premetamorphosis larvae, the range of tested concentrations was extended to very low concentrations (down to 0.0001 mg/L) to examine whether recent findings of greater mortality at lower doses than at higher doses were also observed in R. arenarum but no such pattern was found. Exposure of prometamorphosis larvae to concentrations of atrazine of 10 mg/L and above widely prevented completion of metamorphosis and caused important mortality. Alternatively, whereas all animals eventually completed metamorphosis when exposed to concentrations of atrazine between 0.1 and 5 mg/L, the timings of metamorphosis were altered starting from 0.1 mg/L, the lowest concentration tested. Indeed, a significant decrease in the time needed for 50% of the larvae to reach the metamorphic climax (stage 42) was observed within this range of atrazine concentrations, the response presenting a U-shaped non-monotonic dose¡Vresponse curve. Larvae exposed to these concentrations of atrazine also needed significantly more time for completing tail resorption, this effect being equivalent at all concentrations. Overall, the combination of these two different facets of atrazine influence on metamorphosis resulted in a significant acceleration of metamorphosis at 1 mg/L and a significant increase in the duration of metamorphosis at 5 mg/L, whereas no significant difference was observed with 0.1 mg/L.
Fil: Brodeur, Celine Marie Julie. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Svartz, Gabriela Veronica. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
pesticides
atrazine
amphibian development
stage dependent susceptibility
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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CONICET Digital (CONICET)
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Consejo Nacional de Investigaciones Científicas y Técnicas
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Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorptionBrodeur, Celine Marie JulieSvartz, Gabriela VeronicaPerez Coll, Cristina SilviaMarino, Damian Jose GabrielHerkovits, Jorgepesticidesatrazineamphibian developmentstage dependent susceptibilityhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Acute and subchronic toxicity of atrazine was evaluated in embryos (stage 4) and in premetamorphosis (stage 25) and prometamorphosis (stage 38¡V39) larvae of the common South American toad Rhinella arenarum (Anura: bufonidae). The influence of atrazine on the last stages of metamorphosis was also examined by exposing prometamorphosis larvae until completion of metamorphosis. Results obtained revealed that larvae in premetamorphosis are more sensitive than larvae in prometamorphosis and that these are, in turn, more sensitive than embryonic stages. Indeed, concentrations of atrazine as high as 30 mg/L had little effects on embryonic stages, the embryos surviving and developing in a similar manner as controls. LC50s of premetamorphosis larvae equaled 27.16, 7.03 and 2.32 mg/L of atrazine after 4, 14 and 21 days of exposure, respectively, compared to LC50s values of 18.27 and 14.43 mg/L after 14 and 21 days of exposure for larvae in prometamorphosis. In experiments with premetamorphosis larvae, the range of tested concentrations was extended to very low concentrations (down to 0.0001 mg/L) to examine whether recent findings of greater mortality at lower doses than at higher doses were also observed in R. arenarum but no such pattern was found. Exposure of prometamorphosis larvae to concentrations of atrazine of 10 mg/L and above widely prevented completion of metamorphosis and caused important mortality. Alternatively, whereas all animals eventually completed metamorphosis when exposed to concentrations of atrazine between 0.1 and 5 mg/L, the timings of metamorphosis were altered starting from 0.1 mg/L, the lowest concentration tested. Indeed, a significant decrease in the time needed for 50% of the larvae to reach the metamorphic climax (stage 42) was observed within this range of atrazine concentrations, the response presenting a U-shaped non-monotonic dose¡Vresponse curve. Larvae exposed to these concentrations of atrazine also needed significantly more time for completing tail resorption, this effect being equivalent at all concentrations. Overall, the combination of these two different facets of atrazine influence on metamorphosis resulted in a significant acceleration of metamorphosis at 1 mg/L and a significant increase in the duration of metamorphosis at 5 mg/L, whereas no significant difference was observed with 0.1 mg/L.Fil: Brodeur, Celine Marie Julie. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; ArgentinaFil: Svartz, Gabriela Veronica. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; ArgentinaFil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243327Brodeur, Celine Marie Julie; Svartz, Gabriela Veronica; Perez Coll, Cristina Silvia; Marino, Damian Jose Gabriel; Herkovits, Jorge; Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption; Elsevier Science; Aquatic Toxicology; 91; 2; 4-2009; 161-1700166-445XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166445X08002130info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2008.07.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:25:38Zoai:ri.conicet.gov.ar:11336/243327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:25:39.061CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
title Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
spellingShingle Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
Brodeur, Celine Marie Julie
pesticides
atrazine
amphibian development
stage dependent susceptibility
title_short Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
title_full Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
title_fullStr Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
title_full_unstemmed Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
title_sort Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption
dc.creator.none.fl_str_mv Brodeur, Celine Marie Julie
Svartz, Gabriela Veronica
Perez Coll, Cristina Silvia
Marino, Damian Jose Gabriel
Herkovits, Jorge
author Brodeur, Celine Marie Julie
author_facet Brodeur, Celine Marie Julie
Svartz, Gabriela Veronica
Perez Coll, Cristina Silvia
Marino, Damian Jose Gabriel
Herkovits, Jorge
author_role author
author2 Svartz, Gabriela Veronica
Perez Coll, Cristina Silvia
Marino, Damian Jose Gabriel
Herkovits, Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv pesticides
atrazine
amphibian development
stage dependent susceptibility
topic pesticides
atrazine
amphibian development
stage dependent susceptibility
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acute and subchronic toxicity of atrazine was evaluated in embryos (stage 4) and in premetamorphosis (stage 25) and prometamorphosis (stage 38¡V39) larvae of the common South American toad Rhinella arenarum (Anura: bufonidae). The influence of atrazine on the last stages of metamorphosis was also examined by exposing prometamorphosis larvae until completion of metamorphosis. Results obtained revealed that larvae in premetamorphosis are more sensitive than larvae in prometamorphosis and that these are, in turn, more sensitive than embryonic stages. Indeed, concentrations of atrazine as high as 30 mg/L had little effects on embryonic stages, the embryos surviving and developing in a similar manner as controls. LC50s of premetamorphosis larvae equaled 27.16, 7.03 and 2.32 mg/L of atrazine after 4, 14 and 21 days of exposure, respectively, compared to LC50s values of 18.27 and 14.43 mg/L after 14 and 21 days of exposure for larvae in prometamorphosis. In experiments with premetamorphosis larvae, the range of tested concentrations was extended to very low concentrations (down to 0.0001 mg/L) to examine whether recent findings of greater mortality at lower doses than at higher doses were also observed in R. arenarum but no such pattern was found. Exposure of prometamorphosis larvae to concentrations of atrazine of 10 mg/L and above widely prevented completion of metamorphosis and caused important mortality. Alternatively, whereas all animals eventually completed metamorphosis when exposed to concentrations of atrazine between 0.1 and 5 mg/L, the timings of metamorphosis were altered starting from 0.1 mg/L, the lowest concentration tested. Indeed, a significant decrease in the time needed for 50% of the larvae to reach the metamorphic climax (stage 42) was observed within this range of atrazine concentrations, the response presenting a U-shaped non-monotonic dose¡Vresponse curve. Larvae exposed to these concentrations of atrazine also needed significantly more time for completing tail resorption, this effect being equivalent at all concentrations. Overall, the combination of these two different facets of atrazine influence on metamorphosis resulted in a significant acceleration of metamorphosis at 1 mg/L and a significant increase in the duration of metamorphosis at 5 mg/L, whereas no significant difference was observed with 0.1 mg/L.
Fil: Brodeur, Celine Marie Julie. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Svartz, Gabriela Veronica. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente. Instituto de Ciencias Ambientales y Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Acute and subchronic toxicity of atrazine was evaluated in embryos (stage 4) and in premetamorphosis (stage 25) and prometamorphosis (stage 38¡V39) larvae of the common South American toad Rhinella arenarum (Anura: bufonidae). The influence of atrazine on the last stages of metamorphosis was also examined by exposing prometamorphosis larvae until completion of metamorphosis. Results obtained revealed that larvae in premetamorphosis are more sensitive than larvae in prometamorphosis and that these are, in turn, more sensitive than embryonic stages. Indeed, concentrations of atrazine as high as 30 mg/L had little effects on embryonic stages, the embryos surviving and developing in a similar manner as controls. LC50s of premetamorphosis larvae equaled 27.16, 7.03 and 2.32 mg/L of atrazine after 4, 14 and 21 days of exposure, respectively, compared to LC50s values of 18.27 and 14.43 mg/L after 14 and 21 days of exposure for larvae in prometamorphosis. In experiments with premetamorphosis larvae, the range of tested concentrations was extended to very low concentrations (down to 0.0001 mg/L) to examine whether recent findings of greater mortality at lower doses than at higher doses were also observed in R. arenarum but no such pattern was found. Exposure of prometamorphosis larvae to concentrations of atrazine of 10 mg/L and above widely prevented completion of metamorphosis and caused important mortality. Alternatively, whereas all animals eventually completed metamorphosis when exposed to concentrations of atrazine between 0.1 and 5 mg/L, the timings of metamorphosis were altered starting from 0.1 mg/L, the lowest concentration tested. Indeed, a significant decrease in the time needed for 50% of the larvae to reach the metamorphic climax (stage 42) was observed within this range of atrazine concentrations, the response presenting a U-shaped non-monotonic dose¡Vresponse curve. Larvae exposed to these concentrations of atrazine also needed significantly more time for completing tail resorption, this effect being equivalent at all concentrations. Overall, the combination of these two different facets of atrazine influence on metamorphosis resulted in a significant acceleration of metamorphosis at 1 mg/L and a significant increase in the duration of metamorphosis at 5 mg/L, whereas no significant difference was observed with 0.1 mg/L.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243327
Brodeur, Celine Marie Julie; Svartz, Gabriela Veronica; Perez Coll, Cristina Silvia; Marino, Damian Jose Gabriel; Herkovits, Jorge; Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption; Elsevier Science; Aquatic Toxicology; 91; 2; 4-2009; 161-170
0166-445X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243327
identifier_str_mv Brodeur, Celine Marie Julie; Svartz, Gabriela Veronica; Perez Coll, Cristina Silvia; Marino, Damian Jose Gabriel; Herkovits, Jorge; Comparative susceptibility to atrazine of three developmental stages of Bufo arenarum (amphibia: anura) and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption; Elsevier Science; Aquatic Toxicology; 91; 2; 4-2009; 161-170
0166-445X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2008.07.003
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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