Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes
- Autores
- Setubal, João C.; Dos Santos, Patricia Carolina; Goldman, Barry S.; Ertesvag, Helga; Espin, Guadelupe; Rubio, Luis M.; Valla, Svein; Almeida, Nalvo F.; Balasubramanian, Divya; Cromes, Lindsey; Curatti, Leonardo; Du, Zijin; Godsy, Eric; Goodner, Brad; Hellner Burris, Kaitlyn; Hernandez, José A.; Houmiel, Katherine; Imperial, Juan; Kennedy, Christina; Larson, Timothy J.; Latreille, Phil; Ligon, Lauren S.; Lu, Jing; Mærk, Mali; Miller, Nancy M.; Norton, Stacie; O'Carroll, Ina P.; Paulsen, Ian; Raulfs, Estella C.; Roemer, Rebecca
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of 5,365,318 bp. In order to reconcile an obligate aerobic lifestyle with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement of respiratory proteins. It is able to produce alginate, a polymer that further protects the organism from excess exogenous oxygen, and it has multiple duplications of alginate modification genes, which may alter alginate composition in response to oxygen availability. The genome analysis identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes coding for other oxygen-sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogenase. These findings offer new prospects for the wider application of A. vinelandii as a host for the production and characterization of oxygen-sensitive proteins.
Fil: Setubal, João C.. Virginia Polytechnic Institute; Estados Unidos
Fil: Dos Santos, Patricia Carolina. Wake Forest University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Goldman, Barry S.. Monsanto Company; Estados Unidos
Fil: Ertesvag, Helga. Norwegian University of Science and Technology; Noruega
Fil: Espin, Guadelupe. Universidad Nacional Autónoma de México; México
Fil: Rubio, Luis M.. Instituto Imdea Energia; España
Fil: Valla, Svein. Norwegian University of Science and Technology; Noruega
Fil: Almeida, Nalvo F.. Virginia Polytechnic Institute; Estados Unidos. Universidade Federal do Mato Grosso do Sul; Brasil
Fil: Balasubramanian, Divya. Hiram College; Estados Unidos
Fil: Cromes, Lindsey. Hiram College; Estados Unidos
Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Du, Zijin. Monsanto Company; Estados Unidos
Fil: Godsy, Eric. Monsanto Company; Estados Unidos
Fil: Goodner, Brad. Hiram College; Estados Unidos
Fil: Hellner Burris, Kaitlyn. Hiram College; Estados Unidos
Fil: Hernandez, José A.. Midwestern University; Estados Unidos
Fil: Houmiel, Katherine. Seattle Pacific University; Estados Unidos
Fil: Imperial, Juan. Centro de Biotecnologia y Genomica de Plantas; España
Fil: Kennedy, Christina. University of Arizona; Estados Unidos
Fil: Larson, Timothy J.. Virginia Polytechnic Institute; Estados Unidos
Fil: Latreille, Phil. Monsanto Company; Estados Unidos
Fil: Ligon, Lauren S.. Virginia Polytechnic Institute; Estados Unidos
Fil: Lu, Jing. Monsanto Company; Estados Unidos
Fil: Mærk, Mali. Norwegian University of Science and Technology; Noruega
Fil: Miller, Nancy M.. Monsanto Company; Estados Unidos
Fil: Norton, Stacie. Monsanto Company; Estados Unidos
Fil: O'Carroll, Ina P.. Virginia Polytechnic Institute; Estados Unidos
Fil: Paulsen, Ian. Macquarie University; Australia
Fil: Raulfs, Estella C.. Virginia Polytechnic Institute; Estados Unidos
Fil: Roemer, Rebecca. Hiram College; Estados Unidos - Materia
-
AZOTOBACTER
NITROGEN FIXATION
ANAEROBIC PROCESS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/153327
Ver los metadatos del registro completo
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Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic ProcessesSetubal, João C.Dos Santos, Patricia CarolinaGoldman, Barry S.Ertesvag, HelgaEspin, GuadelupeRubio, Luis M.Valla, SveinAlmeida, Nalvo F.Balasubramanian, DivyaCromes, LindseyCuratti, LeonardoDu, ZijinGodsy, EricGoodner, BradHellner Burris, KaitlynHernandez, José A.Houmiel, KatherineImperial, JuanKennedy, ChristinaLarson, Timothy J.Latreille, PhilLigon, Lauren S.Lu, JingMærk, MaliMiller, Nancy M.Norton, StacieO'Carroll, Ina P.Paulsen, IanRaulfs, Estella C.Roemer, RebeccaAZOTOBACTERNITROGEN FIXATIONANAEROBIC PROCESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of 5,365,318 bp. In order to reconcile an obligate aerobic lifestyle with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement of respiratory proteins. It is able to produce alginate, a polymer that further protects the organism from excess exogenous oxygen, and it has multiple duplications of alginate modification genes, which may alter alginate composition in response to oxygen availability. The genome analysis identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes coding for other oxygen-sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogenase. These findings offer new prospects for the wider application of A. vinelandii as a host for the production and characterization of oxygen-sensitive proteins.Fil: Setubal, João C.. Virginia Polytechnic Institute; Estados UnidosFil: Dos Santos, Patricia Carolina. Wake Forest University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Goldman, Barry S.. Monsanto Company; Estados UnidosFil: Ertesvag, Helga. Norwegian University of Science and Technology; NoruegaFil: Espin, Guadelupe. Universidad Nacional Autónoma de México; MéxicoFil: Rubio, Luis M.. Instituto Imdea Energia; EspañaFil: Valla, Svein. Norwegian University of Science and Technology; NoruegaFil: Almeida, Nalvo F.. Virginia Polytechnic Institute; Estados Unidos. Universidade Federal do Mato Grosso do Sul; BrasilFil: Balasubramanian, Divya. Hiram College; Estados UnidosFil: Cromes, Lindsey. Hiram College; Estados UnidosFil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; ArgentinaFil: Du, Zijin. Monsanto Company; Estados UnidosFil: Godsy, Eric. Monsanto Company; Estados UnidosFil: Goodner, Brad. Hiram College; Estados UnidosFil: Hellner Burris, Kaitlyn. Hiram College; Estados UnidosFil: Hernandez, José A.. Midwestern University; Estados UnidosFil: Houmiel, Katherine. Seattle Pacific University; Estados UnidosFil: Imperial, Juan. Centro de Biotecnologia y Genomica de Plantas; EspañaFil: Kennedy, Christina. University of Arizona; Estados UnidosFil: Larson, Timothy J.. Virginia Polytechnic Institute; Estados UnidosFil: Latreille, Phil. Monsanto Company; Estados UnidosFil: Ligon, Lauren S.. Virginia Polytechnic Institute; Estados UnidosFil: Lu, Jing. Monsanto Company; Estados UnidosFil: Mærk, Mali. Norwegian University of Science and Technology; NoruegaFil: Miller, Nancy M.. Monsanto Company; Estados UnidosFil: Norton, Stacie. Monsanto Company; Estados UnidosFil: O'Carroll, Ina P.. Virginia Polytechnic Institute; Estados UnidosFil: Paulsen, Ian. Macquarie University; AustraliaFil: Raulfs, Estella C.. Virginia Polytechnic Institute; Estados UnidosFil: Roemer, Rebecca. Hiram College; Estados UnidosAmerican Society for Microbiology2009-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153327Setubal, João C.; Dos Santos, Patricia Carolina; Goldman, Barry S.; Ertesvag, Helga; Espin, Guadelupe; et al.; Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes; American Society for Microbiology; Journal of Bacteriology; 191; 14; 15-7-2009; 4534-45450021-91931098-5530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/JB.00504-09info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/JB.00504-09info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:56:26Zoai:ri.conicet.gov.ar:11336/153327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:56:26.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| title |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| spellingShingle |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes Setubal, João C. AZOTOBACTER NITROGEN FIXATION ANAEROBIC PROCESS |
| title_short |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| title_full |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| title_fullStr |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| title_full_unstemmed |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| title_sort |
Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes |
| dc.creator.none.fl_str_mv |
Setubal, João C. Dos Santos, Patricia Carolina Goldman, Barry S. Ertesvag, Helga Espin, Guadelupe Rubio, Luis M. Valla, Svein Almeida, Nalvo F. Balasubramanian, Divya Cromes, Lindsey Curatti, Leonardo Du, Zijin Godsy, Eric Goodner, Brad Hellner Burris, Kaitlyn Hernandez, José A. Houmiel, Katherine Imperial, Juan Kennedy, Christina Larson, Timothy J. Latreille, Phil Ligon, Lauren S. Lu, Jing Mærk, Mali Miller, Nancy M. Norton, Stacie O'Carroll, Ina P. Paulsen, Ian Raulfs, Estella C. Roemer, Rebecca |
| author |
Setubal, João C. |
| author_facet |
Setubal, João C. Dos Santos, Patricia Carolina Goldman, Barry S. Ertesvag, Helga Espin, Guadelupe Rubio, Luis M. Valla, Svein Almeida, Nalvo F. Balasubramanian, Divya Cromes, Lindsey Curatti, Leonardo Du, Zijin Godsy, Eric Goodner, Brad Hellner Burris, Kaitlyn Hernandez, José A. Houmiel, Katherine Imperial, Juan Kennedy, Christina Larson, Timothy J. Latreille, Phil Ligon, Lauren S. Lu, Jing Mærk, Mali Miller, Nancy M. Norton, Stacie O'Carroll, Ina P. Paulsen, Ian Raulfs, Estella C. Roemer, Rebecca |
| author_role |
author |
| author2 |
Dos Santos, Patricia Carolina Goldman, Barry S. Ertesvag, Helga Espin, Guadelupe Rubio, Luis M. Valla, Svein Almeida, Nalvo F. Balasubramanian, Divya Cromes, Lindsey Curatti, Leonardo Du, Zijin Godsy, Eric Goodner, Brad Hellner Burris, Kaitlyn Hernandez, José A. Houmiel, Katherine Imperial, Juan Kennedy, Christina Larson, Timothy J. Latreille, Phil Ligon, Lauren S. Lu, Jing Mærk, Mali Miller, Nancy M. Norton, Stacie O'Carroll, Ina P. Paulsen, Ian Raulfs, Estella C. Roemer, Rebecca |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
AZOTOBACTER NITROGEN FIXATION ANAEROBIC PROCESS |
| topic |
AZOTOBACTER NITROGEN FIXATION ANAEROBIC PROCESS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of 5,365,318 bp. In order to reconcile an obligate aerobic lifestyle with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement of respiratory proteins. It is able to produce alginate, a polymer that further protects the organism from excess exogenous oxygen, and it has multiple duplications of alginate modification genes, which may alter alginate composition in response to oxygen availability. The genome analysis identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes coding for other oxygen-sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogenase. These findings offer new prospects for the wider application of A. vinelandii as a host for the production and characterization of oxygen-sensitive proteins. Fil: Setubal, João C.. Virginia Polytechnic Institute; Estados Unidos Fil: Dos Santos, Patricia Carolina. Wake Forest University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina Fil: Goldman, Barry S.. Monsanto Company; Estados Unidos Fil: Ertesvag, Helga. Norwegian University of Science and Technology; Noruega Fil: Espin, Guadelupe. Universidad Nacional Autónoma de México; México Fil: Rubio, Luis M.. Instituto Imdea Energia; España Fil: Valla, Svein. Norwegian University of Science and Technology; Noruega Fil: Almeida, Nalvo F.. Virginia Polytechnic Institute; Estados Unidos. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Balasubramanian, Divya. Hiram College; Estados Unidos Fil: Cromes, Lindsey. Hiram College; Estados Unidos Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina Fil: Du, Zijin. Monsanto Company; Estados Unidos Fil: Godsy, Eric. Monsanto Company; Estados Unidos Fil: Goodner, Brad. Hiram College; Estados Unidos Fil: Hellner Burris, Kaitlyn. Hiram College; Estados Unidos Fil: Hernandez, José A.. Midwestern University; Estados Unidos Fil: Houmiel, Katherine. Seattle Pacific University; Estados Unidos Fil: Imperial, Juan. Centro de Biotecnologia y Genomica de Plantas; España Fil: Kennedy, Christina. University of Arizona; Estados Unidos Fil: Larson, Timothy J.. Virginia Polytechnic Institute; Estados Unidos Fil: Latreille, Phil. Monsanto Company; Estados Unidos Fil: Ligon, Lauren S.. Virginia Polytechnic Institute; Estados Unidos Fil: Lu, Jing. Monsanto Company; Estados Unidos Fil: Mærk, Mali. Norwegian University of Science and Technology; Noruega Fil: Miller, Nancy M.. Monsanto Company; Estados Unidos Fil: Norton, Stacie. Monsanto Company; Estados Unidos Fil: O'Carroll, Ina P.. Virginia Polytechnic Institute; Estados Unidos Fil: Paulsen, Ian. Macquarie University; Australia Fil: Raulfs, Estella C.. Virginia Polytechnic Institute; Estados Unidos Fil: Roemer, Rebecca. Hiram College; Estados Unidos |
| description |
Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of 5,365,318 bp. In order to reconcile an obligate aerobic lifestyle with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement of respiratory proteins. It is able to produce alginate, a polymer that further protects the organism from excess exogenous oxygen, and it has multiple duplications of alginate modification genes, which may alter alginate composition in response to oxygen availability. The genome analysis identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes coding for other oxygen-sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogenase. These findings offer new prospects for the wider application of A. vinelandii as a host for the production and characterization of oxygen-sensitive proteins. |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009-07-15 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/153327 Setubal, João C.; Dos Santos, Patricia Carolina; Goldman, Barry S.; Ertesvag, Helga; Espin, Guadelupe; et al.; Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes; American Society for Microbiology; Journal of Bacteriology; 191; 14; 15-7-2009; 4534-4545 0021-9193 1098-5530 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/153327 |
| identifier_str_mv |
Setubal, João C.; Dos Santos, Patricia Carolina; Goldman, Barry S.; Ertesvag, Helga; Espin, Guadelupe; et al.; Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes; American Society for Microbiology; Journal of Bacteriology; 191; 14; 15-7-2009; 4534-4545 0021-9193 1098-5530 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.00504-09 info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/JB.00504-09 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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application/pdf application/pdf |
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American Society for Microbiology |
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American Society for Microbiology |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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