Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat
- Autores
- Liu, Li; Ikeda, Tatsuya M.; Branlard, Gerard; Peña, Roberto J.; Rogers, William John; Lerner, Silvia E.; Kolman, Maria de Los Angeles; Xia, Xianchun; Wang, Linhai; Ma, Wujun; Appels, Rudi; Yoshida, Hisashi; Wang, Aili; Yan, Yueming; He, Zhonghu
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. Results At the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c, Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles. Conclusions PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat.
Fil: Liu, Li. Chinese Academy of Agricultural Sciences; China
Fil: Ikeda, Tatsuya M.. National Agriculture and Food Research Organization; Japón
Fil: Branlard, Gerard. Institut National de la Recherche Agronomique; Francia
Fil: Peña, Roberto J.. Centro Internacional de Mejoramiento de Maíz y Trigo; México
Fil: Rogers, William John. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lerner, Silvia E.. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Kolman, Maria de Los Angeles. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Xia, Xianchun. Chinese Academy of Agricultural Sciences; China
Fil: Wang, Linhai. Chinese Academy of Agricultural Sciences; China
Fil: Ma, Wujun. Murdoch University; Australia
Fil: Appels, Rudi. Murdoch University; Australia
Fil: Yoshida, Hisashi. National Agriculture and Food Research Organization; Japón
Fil: Wang, Aili. Capital Normal University; China
Fil: Yan, Yueming. Capital Normal University; China
Fil: He, Zhonghu. Chinese Academy of Agricultural Sciences; China. Centro Internacional de Mejoramiento de Maíz y Trigo; México - Materia
-
bread wheat
low molecular weight glutenin
SDS-PAGE
2-DE
MALDI-TOF-MS
PCR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/15870
Ver los metadatos del registro completo
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Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheatLiu, LiIkeda, Tatsuya M.Branlard, GerardPeña, Roberto J.Rogers, William JohnLerner, Silvia E.Kolman, Maria de Los AngelesXia, XianchunWang, LinhaiMa, WujunAppels, RudiYoshida, HisashiWang, AiliYan, YuemingHe, Zhonghubread wheatlow molecular weight gluteninSDS-PAGE2-DEMALDI-TOF-MSPCRhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Background Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. Results At the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c, Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles. Conclusions PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat.Fil: Liu, Li. Chinese Academy of Agricultural Sciences; ChinaFil: Ikeda, Tatsuya M.. National Agriculture and Food Research Organization; JapónFil: Branlard, Gerard. Institut National de la Recherche Agronomique; FranciaFil: Peña, Roberto J.. Centro Internacional de Mejoramiento de Maíz y Trigo; MéxicoFil: Rogers, William John. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lerner, Silvia E.. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Kolman, Maria de Los Angeles. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Xia, Xianchun. Chinese Academy of Agricultural Sciences; ChinaFil: Wang, Linhai. Chinese Academy of Agricultural Sciences; ChinaFil: Ma, Wujun. Murdoch University; AustraliaFil: Appels, Rudi. Murdoch University; AustraliaFil: Yoshida, Hisashi. National Agriculture and Food Research Organization; JapónFil: Wang, Aili. Capital Normal University; ChinaFil: Yan, Yueming. Capital Normal University; ChinaFil: He, Zhonghu. Chinese Academy of Agricultural Sciences; China. Centro Internacional de Mejoramiento de Maíz y Trigo; MéxicoBiomed Central2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15870Liu, Li; Ikeda, Tatsuya M.; Branlard, Gerard; Peña, Roberto J.; Rogers, William John; et al.; Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat; Biomed Central; Bmc Plant Biology; 10; 6-2010; 124-1481471-2229enginfo:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2229/10/124info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-10-124info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:30Zoai:ri.conicet.gov.ar:11336/15870instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:30.895CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| title |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| spellingShingle |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat Liu, Li bread wheat low molecular weight glutenin SDS-PAGE 2-DE MALDI-TOF-MS PCR |
| title_short |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| title_full |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| title_fullStr |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| title_full_unstemmed |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| title_sort |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| dc.creator.none.fl_str_mv |
Liu, Li Ikeda, Tatsuya M. Branlard, Gerard Peña, Roberto J. Rogers, William John Lerner, Silvia E. Kolman, Maria de Los Angeles Xia, Xianchun Wang, Linhai Ma, Wujun Appels, Rudi Yoshida, Hisashi Wang, Aili Yan, Yueming He, Zhonghu |
| author |
Liu, Li |
| author_facet |
Liu, Li Ikeda, Tatsuya M. Branlard, Gerard Peña, Roberto J. Rogers, William John Lerner, Silvia E. Kolman, Maria de Los Angeles Xia, Xianchun Wang, Linhai Ma, Wujun Appels, Rudi Yoshida, Hisashi Wang, Aili Yan, Yueming He, Zhonghu |
| author_role |
author |
| author2 |
Ikeda, Tatsuya M. Branlard, Gerard Peña, Roberto J. Rogers, William John Lerner, Silvia E. Kolman, Maria de Los Angeles Xia, Xianchun Wang, Linhai Ma, Wujun Appels, Rudi Yoshida, Hisashi Wang, Aili Yan, Yueming He, Zhonghu |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
bread wheat low molecular weight glutenin SDS-PAGE 2-DE MALDI-TOF-MS PCR |
| topic |
bread wheat low molecular weight glutenin SDS-PAGE 2-DE MALDI-TOF-MS PCR |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| dc.description.none.fl_txt_mv |
Background Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. Results At the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c, Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles. Conclusions PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat. Fil: Liu, Li. Chinese Academy of Agricultural Sciences; China Fil: Ikeda, Tatsuya M.. National Agriculture and Food Research Organization; Japón Fil: Branlard, Gerard. Institut National de la Recherche Agronomique; Francia Fil: Peña, Roberto J.. Centro Internacional de Mejoramiento de Maíz y Trigo; México Fil: Rogers, William John. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lerner, Silvia E.. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina Fil: Kolman, Maria de Los Angeles. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Xia, Xianchun. Chinese Academy of Agricultural Sciences; China Fil: Wang, Linhai. Chinese Academy of Agricultural Sciences; China Fil: Ma, Wujun. Murdoch University; Australia Fil: Appels, Rudi. Murdoch University; Australia Fil: Yoshida, Hisashi. National Agriculture and Food Research Organization; Japón Fil: Wang, Aili. Capital Normal University; China Fil: Yan, Yueming. Capital Normal University; China Fil: He, Zhonghu. Chinese Academy of Agricultural Sciences; China. Centro Internacional de Mejoramiento de Maíz y Trigo; México |
| description |
Background Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. Results At the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c, Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles. Conclusions PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/15870 Liu, Li; Ikeda, Tatsuya M.; Branlard, Gerard; Peña, Roberto J.; Rogers, William John; et al.; Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat; Biomed Central; Bmc Plant Biology; 10; 6-2010; 124-148 1471-2229 |
| url |
http://hdl.handle.net/11336/15870 |
| identifier_str_mv |
Liu, Li; Ikeda, Tatsuya M.; Branlard, Gerard; Peña, Roberto J.; Rogers, William John; et al.; Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat; Biomed Central; Bmc Plant Biology; 10; 6-2010; 124-148 1471-2229 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2229/10/124 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-10-124 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by/2.5/ar/ |
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application/pdf application/pdf application/pdf |
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Biomed Central |
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Biomed Central |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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