B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness
- Autores
- Carriere, Pedro Matias; Novoa Díaz, María Belén; Birkenstok, Cintia Noelia; Picardi, Gonzalo; Sica, Gabriela; Calvo, Natalia Graciela; Vinderola, Celso Gabriel; Gentili, Claudia Rosana
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The human milk-derived strain Bifidobacterium animalis subsp. lactis INL1 (B. lactis INL1) (agreement UNS-UNL N°REC-1092496-2) displays properties of a novel probiotic. Previously we showed that in colorectal cancer (CRC) cells, certain probiotics modulate gene expression associated with macrophage activity and that B. lactis INL1 improves macrophage physiology under lipopolysaccharide (LPS) action. Herein we investigate if B. lactis INL1 is able to modulate: 1- the macrophage activity in an inflammatory context and 2- the interaction between macrophages and CRC cells (which are under a pro-tumor factor action). We observed that pretreatment with 5% of cell-free supernatant (CFS) from B. lactis INL1 for 3 h (condition that not affect cell viability) enhances the migratory and phagocytic capacity of RAW264.7 macrophages promoted by LPS exposure for 24 h. Previously we showed that parathyroid hormone-related peptide (PTHrP) is a pro-tumor factor that favors HCT116 cells aggressiveness via E-cadherin deregulation. Also, our in silico analysis revealed that the macrophage inhibitory cytokine GDF15 is overexpressed in HCT116 cells. In view of these findings, we next proceeded to evaluate the expression of both markers. Western blot analysis revealed that the pre-treatment of HCT116 cells with CFS from B. lactis INL1 followed by PTHrP exposition for 24 h reverses the changes in E-cadherin and GDF15 expression induced by PTHrP. Finally, we evaluated whether the conditioned medium from HCT116 cells pre-treated with B. lactis INL1 CFS (MCT-T) and exposed to PTHrP affects macrophage function. Using a viability assay, we observed that MCT-T enhanced macrophage viability compared to conditioned medium from cells under PTHrP action but untreated with probiotic. These results suggest that expression and secretion of GDF15 from tumor cells may mediate the suppressive effects of tumor conditioned medium on immune cells, and that B. lactis INL1 could modulate this process.
Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Birkenstok, Cintia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Picardi, Gonzalo. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina
Fil: Sica, Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina
Fil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y XXVI Sociedad Argentina de Fisiología
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Fisiología
Asociación Latinoamericana de Ciencias Fisiológicas - Materia
-
B. LACTIS INL1
MACROPHAGES
COLORECTAL CANCER - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/264234
Ver los metadatos del registro completo
id |
CONICETDig_9614a90c2fb0f991ce553e502c38b599 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/264234 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressivenessCarriere, Pedro MatiasNovoa Díaz, María BelénBirkenstok, Cintia NoeliaPicardi, GonzaloSica, GabrielaCalvo, Natalia GracielaVinderola, Celso GabrielGentili, Claudia RosanaB. LACTIS INL1MACROPHAGESCOLORECTAL CANCERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The human milk-derived strain Bifidobacterium animalis subsp. lactis INL1 (B. lactis INL1) (agreement UNS-UNL N°REC-1092496-2) displays properties of a novel probiotic. Previously we showed that in colorectal cancer (CRC) cells, certain probiotics modulate gene expression associated with macrophage activity and that B. lactis INL1 improves macrophage physiology under lipopolysaccharide (LPS) action. Herein we investigate if B. lactis INL1 is able to modulate: 1- the macrophage activity in an inflammatory context and 2- the interaction between macrophages and CRC cells (which are under a pro-tumor factor action). We observed that pretreatment with 5% of cell-free supernatant (CFS) from B. lactis INL1 for 3 h (condition that not affect cell viability) enhances the migratory and phagocytic capacity of RAW264.7 macrophages promoted by LPS exposure for 24 h. Previously we showed that parathyroid hormone-related peptide (PTHrP) is a pro-tumor factor that favors HCT116 cells aggressiveness via E-cadherin deregulation. Also, our in silico analysis revealed that the macrophage inhibitory cytokine GDF15 is overexpressed in HCT116 cells. In view of these findings, we next proceeded to evaluate the expression of both markers. Western blot analysis revealed that the pre-treatment of HCT116 cells with CFS from B. lactis INL1 followed by PTHrP exposition for 24 h reverses the changes in E-cadherin and GDF15 expression induced by PTHrP. Finally, we evaluated whether the conditioned medium from HCT116 cells pre-treated with B. lactis INL1 CFS (MCT-T) and exposed to PTHrP affects macrophage function. Using a viability assay, we observed that MCT-T enhanced macrophage viability compared to conditioned medium from cells under PTHrP action but untreated with probiotic. These results suggest that expression and secretion of GDF15 from tumor cells may mediate the suppressive effects of tumor conditioned medium on immune cells, and that B. lactis INL1 could modulate this process.Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Birkenstok, Cintia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Picardi, Gonzalo. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; ArgentinaFil: Sica, Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; ArgentinaFil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y XXVI Sociedad Argentina de FisiologíaCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de FisiologíaAsociación Latinoamericana de Ciencias FisiológicasFundación Revista MedicinaRey, Rodolfo2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264234B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness; LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y XXVI Sociedad Argentina de Fisiología; Ciudad Autónoma de Buenos Aires; Argentina; 2024; 165 - 1651669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:28Zoai:ri.conicet.gov.ar:11336/264234instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:28.848CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
title |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
spellingShingle |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness Carriere, Pedro Matias B. LACTIS INL1 MACROPHAGES COLORECTAL CANCER |
title_short |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
title_full |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
title_fullStr |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
title_full_unstemmed |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
title_sort |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness |
dc.creator.none.fl_str_mv |
Carriere, Pedro Matias Novoa Díaz, María Belén Birkenstok, Cintia Noelia Picardi, Gonzalo Sica, Gabriela Calvo, Natalia Graciela Vinderola, Celso Gabriel Gentili, Claudia Rosana |
author |
Carriere, Pedro Matias |
author_facet |
Carriere, Pedro Matias Novoa Díaz, María Belén Birkenstok, Cintia Noelia Picardi, Gonzalo Sica, Gabriela Calvo, Natalia Graciela Vinderola, Celso Gabriel Gentili, Claudia Rosana |
author_role |
author |
author2 |
Novoa Díaz, María Belén Birkenstok, Cintia Noelia Picardi, Gonzalo Sica, Gabriela Calvo, Natalia Graciela Vinderola, Celso Gabriel Gentili, Claudia Rosana |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Rey, Rodolfo |
dc.subject.none.fl_str_mv |
B. LACTIS INL1 MACROPHAGES COLORECTAL CANCER |
topic |
B. LACTIS INL1 MACROPHAGES COLORECTAL CANCER |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The human milk-derived strain Bifidobacterium animalis subsp. lactis INL1 (B. lactis INL1) (agreement UNS-UNL N°REC-1092496-2) displays properties of a novel probiotic. Previously we showed that in colorectal cancer (CRC) cells, certain probiotics modulate gene expression associated with macrophage activity and that B. lactis INL1 improves macrophage physiology under lipopolysaccharide (LPS) action. Herein we investigate if B. lactis INL1 is able to modulate: 1- the macrophage activity in an inflammatory context and 2- the interaction between macrophages and CRC cells (which are under a pro-tumor factor action). We observed that pretreatment with 5% of cell-free supernatant (CFS) from B. lactis INL1 for 3 h (condition that not affect cell viability) enhances the migratory and phagocytic capacity of RAW264.7 macrophages promoted by LPS exposure for 24 h. Previously we showed that parathyroid hormone-related peptide (PTHrP) is a pro-tumor factor that favors HCT116 cells aggressiveness via E-cadherin deregulation. Also, our in silico analysis revealed that the macrophage inhibitory cytokine GDF15 is overexpressed in HCT116 cells. In view of these findings, we next proceeded to evaluate the expression of both markers. Western blot analysis revealed that the pre-treatment of HCT116 cells with CFS from B. lactis INL1 followed by PTHrP exposition for 24 h reverses the changes in E-cadherin and GDF15 expression induced by PTHrP. Finally, we evaluated whether the conditioned medium from HCT116 cells pre-treated with B. lactis INL1 CFS (MCT-T) and exposed to PTHrP affects macrophage function. Using a viability assay, we observed that MCT-T enhanced macrophage viability compared to conditioned medium from cells under PTHrP action but untreated with probiotic. These results suggest that expression and secretion of GDF15 from tumor cells may mediate the suppressive effects of tumor conditioned medium on immune cells, and that B. lactis INL1 could modulate this process. Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Birkenstok, Cintia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Picardi, Gonzalo. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina Fil: Sica, Gabriela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina Fil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y XXVI Sociedad Argentina de Fisiología Ciudad Autónoma de Buenos Aires Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Fisiología Asociación Latinoamericana de Ciencias Fisiológicas |
description |
The human milk-derived strain Bifidobacterium animalis subsp. lactis INL1 (B. lactis INL1) (agreement UNS-UNL N°REC-1092496-2) displays properties of a novel probiotic. Previously we showed that in colorectal cancer (CRC) cells, certain probiotics modulate gene expression associated with macrophage activity and that B. lactis INL1 improves macrophage physiology under lipopolysaccharide (LPS) action. Herein we investigate if B. lactis INL1 is able to modulate: 1- the macrophage activity in an inflammatory context and 2- the interaction between macrophages and CRC cells (which are under a pro-tumor factor action). We observed that pretreatment with 5% of cell-free supernatant (CFS) from B. lactis INL1 for 3 h (condition that not affect cell viability) enhances the migratory and phagocytic capacity of RAW264.7 macrophages promoted by LPS exposure for 24 h. Previously we showed that parathyroid hormone-related peptide (PTHrP) is a pro-tumor factor that favors HCT116 cells aggressiveness via E-cadherin deregulation. Also, our in silico analysis revealed that the macrophage inhibitory cytokine GDF15 is overexpressed in HCT116 cells. In view of these findings, we next proceeded to evaluate the expression of both markers. Western blot analysis revealed that the pre-treatment of HCT116 cells with CFS from B. lactis INL1 followed by PTHrP exposition for 24 h reverses the changes in E-cadherin and GDF15 expression induced by PTHrP. Finally, we evaluated whether the conditioned medium from HCT116 cells pre-treated with B. lactis INL1 CFS (MCT-T) and exposed to PTHrP affects macrophage function. Using a viability assay, we observed that MCT-T enhanced macrophage viability compared to conditioned medium from cells under PTHrP action but untreated with probiotic. These results suggest that expression and secretion of GDF15 from tumor cells may mediate the suppressive effects of tumor conditioned medium on immune cells, and that B. lactis INL1 could modulate this process. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/264234 B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness; LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y XXVI Sociedad Argentina de Fisiología; Ciudad Autónoma de Buenos Aires; Argentina; 2024; 165 - 165 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/264234 |
identifier_str_mv |
B. lactis INL1 improves macrophage immune function and modulates events associated with colorectal cancer cell aggressiveness; LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y XXVI Sociedad Argentina de Fisiología; Ciudad Autónoma de Buenos Aires; Argentina; 2024; 165 - 165 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Fundación Revista Medicina |
publisher.none.fl_str_mv |
Fundación Revista Medicina |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846083262711070720 |
score |
13.22299 |