Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa

Autores
Rodriguez, P. C.; Valdez, Laura Batriz; Zaobornyj, Tamara; Boveris, Alberto Antonio; Beconi, Martha Teresa
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to quantify NO, O2− and ONOO− production during heparin-induced capacitation of cryopreserved bovine spermatozoa. A time dependent hyperbolic increase was observed for heparin-dependent capacitation, O2 uptake, and NO production. Conversely, O2− production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol/min × 107 cells), an enhancement in NO release (1.1 ± 0.2 nmol/min × 107 cells), and a five-fold increase in O2− production (1.3 ± 0.07 nmol/min × 107 cells), were observed. Peroxynitrite production rate was estimated taking into account NO and O2− generation and the second-order rate constant of the reaction between these species. To conclude, heparin-induced capacitation of cryopreserved bovine spermatozoa activates (i) mitochondrial O2 uptake by high ADP levels due to increased energy requirements, (ii) NO production by a constitutive NOS and (iii) O2− production by a membrane-bound NAD(P)H oxidase. The products of both enzymes are released to the extracellular space and could be involved in the process of sperm capacitation.
Fil: Rodriguez, P. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Valdez, Laura Batriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Zaobornyj, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Beconi, Martha Teresa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; Argentina
Materia
Nitric Oxide Production
Superoxide Anion Production
Bovine Spermatozoa
Heparin-Induced Capacitation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16284
Acceder
id CONICETDig_9521fe634039f94824acf24ff7146201
oai_identifier_str oai:ri.conicet.gov.ar:11336/16284
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine SpermatozoaRodriguez, P. C.Valdez, Laura BatrizZaobornyj, TamaraBoveris, Alberto AntonioBeconi, Martha TeresaNitric Oxide ProductionSuperoxide Anion ProductionBovine SpermatozoaHeparin-Induced Capacitationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this work was to quantify NO, O2− and ONOO− production during heparin-induced capacitation of cryopreserved bovine spermatozoa. A time dependent hyperbolic increase was observed for heparin-dependent capacitation, O2 uptake, and NO production. Conversely, O2− production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol/min × 107 cells), an enhancement in NO release (1.1 ± 0.2 nmol/min × 107 cells), and a five-fold increase in O2− production (1.3 ± 0.07 nmol/min × 107 cells), were observed. Peroxynitrite production rate was estimated taking into account NO and O2− generation and the second-order rate constant of the reaction between these species. To conclude, heparin-induced capacitation of cryopreserved bovine spermatozoa activates (i) mitochondrial O2 uptake by high ADP levels due to increased energy requirements, (ii) NO production by a constitutive NOS and (iii) O2− production by a membrane-bound NAD(P)H oxidase. The products of both enzymes are released to the extracellular space and could be involved in the process of sperm capacitation.Fil: Rodriguez, P. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Valdez, Laura Batriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Zaobornyj, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Beconi, Martha Teresa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; ArgentinaWiley Blackwell Publishing, Inc2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16284Rodriguez, P. C.; Valdez, Laura Batriz; Zaobornyj, Tamara; Boveris, Alberto Antonio; Beconi, Martha Teresa; Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 1; 2-2011; 74-810936-6768enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2010.01583.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2010.01583.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:06:58Zoai:ri.conicet.gov.ar:11336/16284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:06:58.368CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
title Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
spellingShingle Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
Rodriguez, P. C.
Nitric Oxide Production
Superoxide Anion Production
Bovine Spermatozoa
Heparin-Induced Capacitation
title_short Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
title_full Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
title_fullStr Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
title_full_unstemmed Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
title_sort Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa
dc.creator.none.fl_str_mv Rodriguez, P. C.
Valdez, Laura Batriz
Zaobornyj, Tamara
Boveris, Alberto Antonio
Beconi, Martha Teresa
author Rodriguez, P. C.
author_facet Rodriguez, P. C.
Valdez, Laura Batriz
Zaobornyj, Tamara
Boveris, Alberto Antonio
Beconi, Martha Teresa
author_role author
author2 Valdez, Laura Batriz
Zaobornyj, Tamara
Boveris, Alberto Antonio
Beconi, Martha Teresa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Nitric Oxide Production
Superoxide Anion Production
Bovine Spermatozoa
Heparin-Induced Capacitation
topic Nitric Oxide Production
Superoxide Anion Production
Bovine Spermatozoa
Heparin-Induced Capacitation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this work was to quantify NO, O2− and ONOO− production during heparin-induced capacitation of cryopreserved bovine spermatozoa. A time dependent hyperbolic increase was observed for heparin-dependent capacitation, O2 uptake, and NO production. Conversely, O2− production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol/min × 107 cells), an enhancement in NO release (1.1 ± 0.2 nmol/min × 107 cells), and a five-fold increase in O2− production (1.3 ± 0.07 nmol/min × 107 cells), were observed. Peroxynitrite production rate was estimated taking into account NO and O2− generation and the second-order rate constant of the reaction between these species. To conclude, heparin-induced capacitation of cryopreserved bovine spermatozoa activates (i) mitochondrial O2 uptake by high ADP levels due to increased energy requirements, (ii) NO production by a constitutive NOS and (iii) O2− production by a membrane-bound NAD(P)H oxidase. The products of both enzymes are released to the extracellular space and could be involved in the process of sperm capacitation.
Fil: Rodriguez, P. C.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Valdez, Laura Batriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Zaobornyj, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Beconi, Martha Teresa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; Argentina
description The aim of this work was to quantify NO, O2− and ONOO− production during heparin-induced capacitation of cryopreserved bovine spermatozoa. A time dependent hyperbolic increase was observed for heparin-dependent capacitation, O2 uptake, and NO production. Conversely, O2− production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol/min × 107 cells), an enhancement in NO release (1.1 ± 0.2 nmol/min × 107 cells), and a five-fold increase in O2− production (1.3 ± 0.07 nmol/min × 107 cells), were observed. Peroxynitrite production rate was estimated taking into account NO and O2− generation and the second-order rate constant of the reaction between these species. To conclude, heparin-induced capacitation of cryopreserved bovine spermatozoa activates (i) mitochondrial O2 uptake by high ADP levels due to increased energy requirements, (ii) NO production by a constitutive NOS and (iii) O2− production by a membrane-bound NAD(P)H oxidase. The products of both enzymes are released to the extracellular space and could be involved in the process of sperm capacitation.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16284
Rodriguez, P. C.; Valdez, Laura Batriz; Zaobornyj, Tamara; Boveris, Alberto Antonio; Beconi, Martha Teresa; Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 1; 2-2011; 74-81
0936-6768
url http://hdl.handle.net/11336/16284
identifier_str_mv Rodriguez, P. C.; Valdez, Laura Batriz; Zaobornyj, Tamara; Boveris, Alberto Antonio; Beconi, Martha Teresa; Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 1; 2-2011; 74-81
0936-6768
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2010.01583.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2010.01583.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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