Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis

Autores
Silva, Pedro E. A.; Bigi, Fabiana; Santangelo, María de la Paz; Romano, Maria Isabel; Martín, Carlos; Cataldi, Ángel Adrián; Aínsa, José A.
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Mycobacterium bovis P55 gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of Mycobacterium tuberculosis H37Rv, annotated as a probable drug efflux protein. Genes similar to P55 were present in all species of the M. tuberculosis complex and other mycobacteria such as Mycobacterium leprae and Mycobacterium avium. By Western blotting, P55 was located in the membrane fraction of M. bovis. When transformed into Mycobacterium smegmatis after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone and the pump inhibitors verapamil and reserpine. M. smegmatis cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.
Fil: Silva, Pedro E. A.. Universidad de Zaragoza; España
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Martín, Carlos. Universidad de Zaragoza; España
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Aínsa, José A.. Universidad de Zaragoza; España
Materia
Mycobacterium
Bovis
P55
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71775
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71775
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosisSilva, Pedro E. A.Bigi, FabianaSantangelo, María de la PazRomano, Maria IsabelMartín, CarlosCataldi, Ángel AdriánAínsa, José A.MycobacteriumBovisP55https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Mycobacterium bovis P55 gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of Mycobacterium tuberculosis H37Rv, annotated as a probable drug efflux protein. Genes similar to P55 were present in all species of the M. tuberculosis complex and other mycobacteria such as Mycobacterium leprae and Mycobacterium avium. By Western blotting, P55 was located in the membrane fraction of M. bovis. When transformed into Mycobacterium smegmatis after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone and the pump inhibitors verapamil and reserpine. M. smegmatis cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.Fil: Silva, Pedro E. A.. Universidad de Zaragoza; EspañaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Martín, Carlos. Universidad de Zaragoza; EspañaFil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Aínsa, José A.. Universidad de Zaragoza; EspañaAmerican Society for Microbiology2001-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71775Silva, Pedro E. A.; Bigi, Fabiana; Santangelo, María de la Paz; Romano, Maria Isabel; Martín, Carlos; et al.; Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 45; 3; 3-2001; 800-8040066-4804CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.45.3.800-804.2001info:eu-repo/semantics/altIdentifier/url/https://aac.asm.org/content/45/3/800info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:14:46Zoai:ri.conicet.gov.ar:11336/71775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:14:46.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
title Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
spellingShingle Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
Silva, Pedro E. A.
Mycobacterium
Bovis
P55
title_short Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
title_full Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
title_fullStr Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
title_full_unstemmed Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
title_sort Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Silva, Pedro E. A.
Bigi, Fabiana
Santangelo, María de la Paz
Romano, Maria Isabel
Martín, Carlos
Cataldi, Ángel Adrián
Aínsa, José A.
author Silva, Pedro E. A.
author_facet Silva, Pedro E. A.
Bigi, Fabiana
Santangelo, María de la Paz
Romano, Maria Isabel
Martín, Carlos
Cataldi, Ángel Adrián
Aínsa, José A.
author_role author
author2 Bigi, Fabiana
Santangelo, María de la Paz
Romano, Maria Isabel
Martín, Carlos
Cataldi, Ángel Adrián
Aínsa, José A.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium
Bovis
P55
topic Mycobacterium
Bovis
P55
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Mycobacterium bovis P55 gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of Mycobacterium tuberculosis H37Rv, annotated as a probable drug efflux protein. Genes similar to P55 were present in all species of the M. tuberculosis complex and other mycobacteria such as Mycobacterium leprae and Mycobacterium avium. By Western blotting, P55 was located in the membrane fraction of M. bovis. When transformed into Mycobacterium smegmatis after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone and the pump inhibitors verapamil and reserpine. M. smegmatis cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.
Fil: Silva, Pedro E. A.. Universidad de Zaragoza; España
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Martín, Carlos. Universidad de Zaragoza; España
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Aínsa, José A.. Universidad de Zaragoza; España
description The Mycobacterium bovis P55 gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of Mycobacterium tuberculosis H37Rv, annotated as a probable drug efflux protein. Genes similar to P55 were present in all species of the M. tuberculosis complex and other mycobacteria such as Mycobacterium leprae and Mycobacterium avium. By Western blotting, P55 was located in the membrane fraction of M. bovis. When transformed into Mycobacterium smegmatis after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone and the pump inhibitors verapamil and reserpine. M. smegmatis cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.
publishDate 2001
dc.date.none.fl_str_mv 2001-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71775
Silva, Pedro E. A.; Bigi, Fabiana; Santangelo, María de la Paz; Romano, Maria Isabel; Martín, Carlos; et al.; Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 45; 3; 3-2001; 800-804
0066-4804
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71775
identifier_str_mv Silva, Pedro E. A.; Bigi, Fabiana; Santangelo, María de la Paz; Romano, Maria Isabel; Martín, Carlos; et al.; Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 45; 3; 3-2001; 800-804
0066-4804
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.45.3.800-804.2001
info:eu-repo/semantics/altIdentifier/url/https://aac.asm.org/content/45/3/800
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781571911122944
score 12.982451

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