Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers

Autores
Pena, Gabriela Alejandra; Coelho, Irene; Reynoso, Maria Marta; Soleiro, Carla; Cavaglieri, Lilia Reneé
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed.
Fil: Pena, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Coelho, Irene. Conselho Nacional de Pesquisas Científicas ; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Soleiro, Carla. Conselho Nacional de Pesquisas Científicas ; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
ANIMAL FEEDS
FEED-BORNE A. FUMIGATUS
GENETIC VARIABILITY ESTIMATION
MOLECULAR TYPING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69392
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69392
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markersPena, Gabriela AlejandraCoelho, IreneReynoso, Maria MartaSoleiro, CarlaCavaglieri, Lilia ReneéANIMAL FEEDSFEED-BORNE A. FUMIGATUSGENETIC VARIABILITY ESTIMATIONMOLECULAR TYPINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed.Fil: Pena, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Coelho, Irene. Conselho Nacional de Pesquisas Científicas ; Brasil. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Soleiro, Carla. Conselho Nacional de Pesquisas Científicas ; Brasil. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaTaylor & Francis Ltd2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69392Pena, Gabriela Alejandra; Coelho, Irene; Reynoso, Maria Marta; Soleiro, Carla; Cavaglieri, Lilia Reneé; Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers; Taylor & Francis Ltd; Medical Mycology; 53; 7; 6-2015; 699-7081369-3786CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myv040info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mmy/article/53/7/699/959666info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:00Zoai:ri.conicet.gov.ar:11336/69392instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:01.142CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
spellingShingle Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
Pena, Gabriela Alejandra
ANIMAL FEEDS
FEED-BORNE A. FUMIGATUS
GENETIC VARIABILITY ESTIMATION
MOLECULAR TYPING
title_short Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_full Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_fullStr Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_full_unstemmed Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_sort Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
dc.creator.none.fl_str_mv Pena, Gabriela Alejandra
Coelho, Irene
Reynoso, Maria Marta
Soleiro, Carla
Cavaglieri, Lilia Reneé
author Pena, Gabriela Alejandra
author_facet Pena, Gabriela Alejandra
Coelho, Irene
Reynoso, Maria Marta
Soleiro, Carla
Cavaglieri, Lilia Reneé
author_role author
author2 Coelho, Irene
Reynoso, Maria Marta
Soleiro, Carla
Cavaglieri, Lilia Reneé
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANIMAL FEEDS
FEED-BORNE A. FUMIGATUS
GENETIC VARIABILITY ESTIMATION
MOLECULAR TYPING
topic ANIMAL FEEDS
FEED-BORNE A. FUMIGATUS
GENETIC VARIABILITY ESTIMATION
MOLECULAR TYPING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed.
Fil: Pena, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Coelho, Irene. Conselho Nacional de Pesquisas Científicas ; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Soleiro, Carla. Conselho Nacional de Pesquisas Científicas ; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69392
Pena, Gabriela Alejandra; Coelho, Irene; Reynoso, Maria Marta; Soleiro, Carla; Cavaglieri, Lilia Reneé; Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers; Taylor & Francis Ltd; Medical Mycology; 53; 7; 6-2015; 699-708
1369-3786
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69392
identifier_str_mv Pena, Gabriela Alejandra; Coelho, Irene; Reynoso, Maria Marta; Soleiro, Carla; Cavaglieri, Lilia Reneé; Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers; Taylor & Francis Ltd; Medical Mycology; 53; 7; 6-2015; 699-708
1369-3786
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myv040
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mmy/article/53/7/699/959666
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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