Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)

Autores
Sabini, Maria Carola; Cariddi, Laura Noelia; Escobar, Franco Matias; Aguilar, Juan Javier; Tonn, Carlos Eugenio; Contigiani de Minio, Marta Silvia; Sabini, Liliana Ines
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It has demonstrated “in vitro” antimicrobial activity exerted by extracts from Minthostachys verticillata (peperina). Objective: Investigate antiviral action of organic extracts of peperina against Herpes simplex virus. Experimental: Vero cells and Herpes simplex virus type 1 strain KOS were used. Aerial vegetal parts of plant were submitted to sequential extraction with n-hexane, chloroform and methanol for 48 h at room temperature. Extracts n-hexanic (N-HE), chloroformic (CE) and methanolic (ME) were obtained. They were added on cell monolayers, and incubated for 48 h at 37°C. Cell viability was determinated by Neutral Red uptake (NRU) after treatment of cells with different concentrations of extracts (10-1000 μg/ml). The same method was performed to evaluate antiviral action exerted by N-HE (100-250 μg/ml), CE (100-500 μg/ml) and ME (range 80-200 μg/ml). Results and discussion: The three extracts showed dose-dependent cytotoxic activity on Vero cells with CC50 values: 502 μg/ml for CE, 244 μg/ml for N-HE and 192 μg/ml for ME. The replication of Herpes simplex virus type 1 “in vitro” was inhibited by each extract at non cytotoxic concentrations. The extracts showed selectivity of action because all concentrations tested inhibited more than 60% of viral production. The results are promising for future natural therapy antiherpetic.
Fil: Sabini, Maria Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Aguilar, Juan Javier. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
MINTHOSTACHYS VERTICILLATA
CYTOTOXICITY
ANTIVIRAL ACTIVITY
ORGANIC EXTRACTS
HERPES SIMPLEX VIRUS
NEUTRAL RED UPTAKE ASSAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109722
Acceder
id CONICETDig_945af989c090c31957a4d197226087e6
oai_identifier_str oai:ri.conicet.gov.ar:11336/109722
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)Sabini, Maria CarolaCariddi, Laura NoeliaEscobar, Franco MatiasAguilar, Juan JavierTonn, Carlos EugenioContigiani de Minio, Marta SilviaSabini, Liliana InesMINTHOSTACHYS VERTICILLATACYTOTOXICITYANTIVIRAL ACTIVITYORGANIC EXTRACTSHERPES SIMPLEX VIRUSNEUTRAL RED UPTAKE ASSAYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It has demonstrated “in vitro” antimicrobial activity exerted by extracts from Minthostachys verticillata (peperina). Objective: Investigate antiviral action of organic extracts of peperina against Herpes simplex virus. Experimental: Vero cells and Herpes simplex virus type 1 strain KOS were used. Aerial vegetal parts of plant were submitted to sequential extraction with n-hexane, chloroform and methanol for 48 h at room temperature. Extracts n-hexanic (N-HE), chloroformic (CE) and methanolic (ME) were obtained. They were added on cell monolayers, and incubated for 48 h at 37°C. Cell viability was determinated by Neutral Red uptake (NRU) after treatment of cells with different concentrations of extracts (10-1000 μg/ml). The same method was performed to evaluate antiviral action exerted by N-HE (100-250 μg/ml), CE (100-500 μg/ml) and ME (range 80-200 μg/ml). Results and discussion: The three extracts showed dose-dependent cytotoxic activity on Vero cells with CC50 values: 502 μg/ml for CE, 244 μg/ml for N-HE and 192 μg/ml for ME. The replication of Herpes simplex virus type 1 “in vitro” was inhibited by each extract at non cytotoxic concentrations. The extracts showed selectivity of action because all concentrations tested inhibited more than 60% of viral production. The results are promising for future natural therapy antiherpetic.Fil: Sabini, Maria Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Cariddi, Laura Noelia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Aguilar, Juan Javier. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaInstituto de Investigación de las Ciencias Exactas Físicas y Naturales2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109722Sabini, Maria Carola; Cariddi, Laura Noelia; Escobar, Franco Matias; Aguilar, Juan Javier; Tonn, Carlos Eugenio; et al.; Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1); Instituto de Investigación de las Ciencias Exactas Físicas y Naturales; Molecular Medicinal Chemistry; 21; 1-2010; 84-871666-888XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.idecefyn.com.ar/MMC21PDF/vol_21__january.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:39:42Zoai:ri.conicet.gov.ar:11336/109722instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:39:43.092CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
title Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
spellingShingle Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
Sabini, Maria Carola
MINTHOSTACHYS VERTICILLATA
CYTOTOXICITY
ANTIVIRAL ACTIVITY
ORGANIC EXTRACTS
HERPES SIMPLEX VIRUS
NEUTRAL RED UPTAKE ASSAY
title_short Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
title_full Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
title_fullStr Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
title_full_unstemmed Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
title_sort Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1)
dc.creator.none.fl_str_mv Sabini, Maria Carola
Cariddi, Laura Noelia
Escobar, Franco Matias
Aguilar, Juan Javier
Tonn, Carlos Eugenio
Contigiani de Minio, Marta Silvia
Sabini, Liliana Ines
author Sabini, Maria Carola
author_facet Sabini, Maria Carola
Cariddi, Laura Noelia
Escobar, Franco Matias
Aguilar, Juan Javier
Tonn, Carlos Eugenio
Contigiani de Minio, Marta Silvia
Sabini, Liliana Ines
author_role author
author2 Cariddi, Laura Noelia
Escobar, Franco Matias
Aguilar, Juan Javier
Tonn, Carlos Eugenio
Contigiani de Minio, Marta Silvia
Sabini, Liliana Ines
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv MINTHOSTACHYS VERTICILLATA
CYTOTOXICITY
ANTIVIRAL ACTIVITY
ORGANIC EXTRACTS
HERPES SIMPLEX VIRUS
NEUTRAL RED UPTAKE ASSAY
topic MINTHOSTACHYS VERTICILLATA
CYTOTOXICITY
ANTIVIRAL ACTIVITY
ORGANIC EXTRACTS
HERPES SIMPLEX VIRUS
NEUTRAL RED UPTAKE ASSAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It has demonstrated “in vitro” antimicrobial activity exerted by extracts from Minthostachys verticillata (peperina). Objective: Investigate antiviral action of organic extracts of peperina against Herpes simplex virus. Experimental: Vero cells and Herpes simplex virus type 1 strain KOS were used. Aerial vegetal parts of plant were submitted to sequential extraction with n-hexane, chloroform and methanol for 48 h at room temperature. Extracts n-hexanic (N-HE), chloroformic (CE) and methanolic (ME) were obtained. They were added on cell monolayers, and incubated for 48 h at 37°C. Cell viability was determinated by Neutral Red uptake (NRU) after treatment of cells with different concentrations of extracts (10-1000 μg/ml). The same method was performed to evaluate antiviral action exerted by N-HE (100-250 μg/ml), CE (100-500 μg/ml) and ME (range 80-200 μg/ml). Results and discussion: The three extracts showed dose-dependent cytotoxic activity on Vero cells with CC50 values: 502 μg/ml for CE, 244 μg/ml for N-HE and 192 μg/ml for ME. The replication of Herpes simplex virus type 1 “in vitro” was inhibited by each extract at non cytotoxic concentrations. The extracts showed selectivity of action because all concentrations tested inhibited more than 60% of viral production. The results are promising for future natural therapy antiherpetic.
Fil: Sabini, Maria Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Cariddi, Laura Noelia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Aguilar, Juan Javier. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description It has demonstrated “in vitro” antimicrobial activity exerted by extracts from Minthostachys verticillata (peperina). Objective: Investigate antiviral action of organic extracts of peperina against Herpes simplex virus. Experimental: Vero cells and Herpes simplex virus type 1 strain KOS were used. Aerial vegetal parts of plant were submitted to sequential extraction with n-hexane, chloroform and methanol for 48 h at room temperature. Extracts n-hexanic (N-HE), chloroformic (CE) and methanolic (ME) were obtained. They were added on cell monolayers, and incubated for 48 h at 37°C. Cell viability was determinated by Neutral Red uptake (NRU) after treatment of cells with different concentrations of extracts (10-1000 μg/ml). The same method was performed to evaluate antiviral action exerted by N-HE (100-250 μg/ml), CE (100-500 μg/ml) and ME (range 80-200 μg/ml). Results and discussion: The three extracts showed dose-dependent cytotoxic activity on Vero cells with CC50 values: 502 μg/ml for CE, 244 μg/ml for N-HE and 192 μg/ml for ME. The replication of Herpes simplex virus type 1 “in vitro” was inhibited by each extract at non cytotoxic concentrations. The extracts showed selectivity of action because all concentrations tested inhibited more than 60% of viral production. The results are promising for future natural therapy antiherpetic.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109722
Sabini, Maria Carola; Cariddi, Laura Noelia; Escobar, Franco Matias; Aguilar, Juan Javier; Tonn, Carlos Eugenio; et al.; Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1); Instituto de Investigación de las Ciencias Exactas Físicas y Naturales; Molecular Medicinal Chemistry; 21; 1-2010; 84-87
1666-888X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109722
identifier_str_mv Sabini, Maria Carola; Cariddi, Laura Noelia; Escobar, Franco Matias; Aguilar, Juan Javier; Tonn, Carlos Eugenio; et al.; Action of extracts obtained with organic solvents from Minthostachys verticillata(Griseb.) Epling on viability of Herpes simplex Type 1 virus (HSV-1); Instituto de Investigación de las Ciencias Exactas Físicas y Naturales; Molecular Medicinal Chemistry; 21; 1-2010; 84-87
1666-888X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.idecefyn.com.ar/MMC21PDF/vol_21__january.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Investigación de las Ciencias Exactas Físicas y Naturales
publisher.none.fl_str_mv Instituto de Investigación de las Ciencias Exactas Físicas y Naturales
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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