Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study

Autores
Lassen, Oscar; Herrera, Jimena María; Dotto, Gladys; Ojeda, Silvia; Garutti, Alicia; Bertolotto, Patricia Isolina; Tabares, Sandra; Sembaj, Adela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, and its activity is mediated by thetype A receptor (EDNRA). This action may play a significant role in the etiology of hypertension.There are different works that shows an association between certain polymorphisms of endothelinaxis and clinical phenotype of hypertension. We describe the genetic variability +138/ex1Short Research ArticleLassen et al.; BJMMR, 11(7): 1-8, 2016; Article no.BJMMR.205202insertion/deletion (I/D) adenosine (A) in the ET-1 gene and polymorphism thymidine/cytosine (T/C)His323His in the EDNRA gene associated at the clinical variability in hypertensive patients.Study Design: Observational, transversal and analytical study.Place and Duration of Study: Hypertension Service at the Internal Medicine Department ofCórdoba Hospital, and Biochemical and Molecular Biology Department in School of Medicine,National University of Cordoba, Argentine. Patients considered hypertensive between April 2009and April 2010.Methodology: Were assessed 136 patients serum lipid profiles, renal and hepatic functions andwere taken Thoracic X-rays, electrocardiograms, and echocardiographs. DNA extracted fromcirculating leukocyte were used to analyze the polymorphisms of genes by PCR-RFLP.Results: For the polymorphisms of Receptor A from Endothelin -1 studied the presence ofcytosine homozygous genotype was less frequent in males (P = .02). For both genders, the samegenotype was associated to low plasma alkaline phosphatase activity and cholesterol levels. Thepresence of thymidine nucleotide allele correlated with plasma alkaline phosphatase activity andcholesterol levels. The Thymidine allele correlated with the degree of cardiovascular compromise(r = 0.54, P= .002). For the genetic variant in the ET-1 gene, the homozygous adenine deletionwas associated to normal plasma levels of glutamate/pyruvate transaminase enzyme activity, uricacid concentration, cholesterol, and Low Density Lipoprotein in hypertensive subjects withoutclinical risk.Conclusion: We observed a gender-specific protective effect for EDNRA gene variations, thesubjects that carried the TT genotype presented more aggressive symptomatology. These resultsshow an association between plasmatic biochemical parameters, the clinical condition, andpolymorphisms in the endothelin axis genes.
Fil: Lassen, Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; Argentina
Fil: Herrera, Jimena María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Dotto, Gladys. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; Argentina
Fil: Ojeda, Silvia. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Garutti, Alicia. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; Argentina
Fil: Bertolotto, Patricia Isolina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Tabares, Sandra. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Sembaj, Adela. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Materia
ENDOTHELIN 1 GENE
ENDOTHELINRECEPTOR A GENE
GENETIC VARIATION
HYPERTENSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23235
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot StudyLassen, OscarHerrera, Jimena MaríaDotto, GladysOjeda, SilviaGarutti, AliciaBertolotto, Patricia IsolinaTabares, SandraSembaj, AdelaENDOTHELIN 1 GENEENDOTHELINRECEPTOR A GENEGENETIC VARIATIONHYPERTENSIONhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, and its activity is mediated by thetype A receptor (EDNRA). This action may play a significant role in the etiology of hypertension.There are different works that shows an association between certain polymorphisms of endothelinaxis and clinical phenotype of hypertension. We describe the genetic variability +138/ex1Short Research ArticleLassen et al.; BJMMR, 11(7): 1-8, 2016; Article no.BJMMR.205202insertion/deletion (I/D) adenosine (A) in the ET-1 gene and polymorphism thymidine/cytosine (T/C)His323His in the EDNRA gene associated at the clinical variability in hypertensive patients.Study Design: Observational, transversal and analytical study.Place and Duration of Study: Hypertension Service at the Internal Medicine Department ofCórdoba Hospital, and Biochemical and Molecular Biology Department in School of Medicine,National University of Cordoba, Argentine. Patients considered hypertensive between April 2009and April 2010.Methodology: Were assessed 136 patients serum lipid profiles, renal and hepatic functions andwere taken Thoracic X-rays, electrocardiograms, and echocardiographs. DNA extracted fromcirculating leukocyte were used to analyze the polymorphisms of genes by PCR-RFLP.Results: For the polymorphisms of Receptor A from Endothelin -1 studied the presence ofcytosine homozygous genotype was less frequent in males (P = .02). For both genders, the samegenotype was associated to low plasma alkaline phosphatase activity and cholesterol levels. Thepresence of thymidine nucleotide allele correlated with plasma alkaline phosphatase activity andcholesterol levels. The Thymidine allele correlated with the degree of cardiovascular compromise(r = 0.54, P= .002). For the genetic variant in the ET-1 gene, the homozygous adenine deletionwas associated to normal plasma levels of glutamate/pyruvate transaminase enzyme activity, uricacid concentration, cholesterol, and Low Density Lipoprotein in hypertensive subjects withoutclinical risk.Conclusion: We observed a gender-specific protective effect for EDNRA gene variations, thesubjects that carried the TT genotype presented more aggressive symptomatology. These resultsshow an association between plasmatic biochemical parameters, the clinical condition, andpolymorphisms in the endothelin axis genes.Fil: Lassen, Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; ArgentinaFil: Herrera, Jimena María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Dotto, Gladys. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; ArgentinaFil: Ojeda, Silvia. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Garutti, Alicia. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; ArgentinaFil: Bertolotto, Patricia Isolina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Tabares, Sandra. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; ArgentinaFil: Sembaj, Adela. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; ArgentinaSciencedomain2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23235Lassen, Oscar; Herrera, Jimena María; Dotto, Gladys; Ojeda, Silvia; Garutti, Alicia; et al.; Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study; Sciencedomain; British Journal of Medicine and Medical Research; 11; 7; 1-2016; 1-82231-0614CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/11732info:eu-repo/semantics/altIdentifier/doi/10.9734/BJMMR/2016/20520info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:04Zoai:ri.conicet.gov.ar:11336/23235instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:04.489CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
title Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
spellingShingle Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
Lassen, Oscar
ENDOTHELIN 1 GENE
ENDOTHELINRECEPTOR A GENE
GENETIC VARIATION
HYPERTENSION
title_short Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
title_full Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
title_fullStr Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
title_full_unstemmed Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
title_sort Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study
dc.creator.none.fl_str_mv Lassen, Oscar
Herrera, Jimena María
Dotto, Gladys
Ojeda, Silvia
Garutti, Alicia
Bertolotto, Patricia Isolina
Tabares, Sandra
Sembaj, Adela
author Lassen, Oscar
author_facet Lassen, Oscar
Herrera, Jimena María
Dotto, Gladys
Ojeda, Silvia
Garutti, Alicia
Bertolotto, Patricia Isolina
Tabares, Sandra
Sembaj, Adela
author_role author
author2 Herrera, Jimena María
Dotto, Gladys
Ojeda, Silvia
Garutti, Alicia
Bertolotto, Patricia Isolina
Tabares, Sandra
Sembaj, Adela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENDOTHELIN 1 GENE
ENDOTHELINRECEPTOR A GENE
GENETIC VARIATION
HYPERTENSION
topic ENDOTHELIN 1 GENE
ENDOTHELINRECEPTOR A GENE
GENETIC VARIATION
HYPERTENSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, and its activity is mediated by thetype A receptor (EDNRA). This action may play a significant role in the etiology of hypertension.There are different works that shows an association between certain polymorphisms of endothelinaxis and clinical phenotype of hypertension. We describe the genetic variability +138/ex1Short Research ArticleLassen et al.; BJMMR, 11(7): 1-8, 2016; Article no.BJMMR.205202insertion/deletion (I/D) adenosine (A) in the ET-1 gene and polymorphism thymidine/cytosine (T/C)His323His in the EDNRA gene associated at the clinical variability in hypertensive patients.Study Design: Observational, transversal and analytical study.Place and Duration of Study: Hypertension Service at the Internal Medicine Department ofCórdoba Hospital, and Biochemical and Molecular Biology Department in School of Medicine,National University of Cordoba, Argentine. Patients considered hypertensive between April 2009and April 2010.Methodology: Were assessed 136 patients serum lipid profiles, renal and hepatic functions andwere taken Thoracic X-rays, electrocardiograms, and echocardiographs. DNA extracted fromcirculating leukocyte were used to analyze the polymorphisms of genes by PCR-RFLP.Results: For the polymorphisms of Receptor A from Endothelin -1 studied the presence ofcytosine homozygous genotype was less frequent in males (P = .02). For both genders, the samegenotype was associated to low plasma alkaline phosphatase activity and cholesterol levels. Thepresence of thymidine nucleotide allele correlated with plasma alkaline phosphatase activity andcholesterol levels. The Thymidine allele correlated with the degree of cardiovascular compromise(r = 0.54, P= .002). For the genetic variant in the ET-1 gene, the homozygous adenine deletionwas associated to normal plasma levels of glutamate/pyruvate transaminase enzyme activity, uricacid concentration, cholesterol, and Low Density Lipoprotein in hypertensive subjects withoutclinical risk.Conclusion: We observed a gender-specific protective effect for EDNRA gene variations, thesubjects that carried the TT genotype presented more aggressive symptomatology. These resultsshow an association between plasmatic biochemical parameters, the clinical condition, andpolymorphisms in the endothelin axis genes.
Fil: Lassen, Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; Argentina
Fil: Herrera, Jimena María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Dotto, Gladys. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; Argentina
Fil: Ojeda, Silvia. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Garutti, Alicia. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Córdoba; Argentina
Fil: Bertolotto, Patricia Isolina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Tabares, Sandra. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
Fil: Sembaj, Adela. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Bioquímica y Biología Molecular; Argentina
description Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, and its activity is mediated by thetype A receptor (EDNRA). This action may play a significant role in the etiology of hypertension.There are different works that shows an association between certain polymorphisms of endothelinaxis and clinical phenotype of hypertension. We describe the genetic variability +138/ex1Short Research ArticleLassen et al.; BJMMR, 11(7): 1-8, 2016; Article no.BJMMR.205202insertion/deletion (I/D) adenosine (A) in the ET-1 gene and polymorphism thymidine/cytosine (T/C)His323His in the EDNRA gene associated at the clinical variability in hypertensive patients.Study Design: Observational, transversal and analytical study.Place and Duration of Study: Hypertension Service at the Internal Medicine Department ofCórdoba Hospital, and Biochemical and Molecular Biology Department in School of Medicine,National University of Cordoba, Argentine. Patients considered hypertensive between April 2009and April 2010.Methodology: Were assessed 136 patients serum lipid profiles, renal and hepatic functions andwere taken Thoracic X-rays, electrocardiograms, and echocardiographs. DNA extracted fromcirculating leukocyte were used to analyze the polymorphisms of genes by PCR-RFLP.Results: For the polymorphisms of Receptor A from Endothelin -1 studied the presence ofcytosine homozygous genotype was less frequent in males (P = .02). For both genders, the samegenotype was associated to low plasma alkaline phosphatase activity and cholesterol levels. Thepresence of thymidine nucleotide allele correlated with plasma alkaline phosphatase activity andcholesterol levels. The Thymidine allele correlated with the degree of cardiovascular compromise(r = 0.54, P= .002). For the genetic variant in the ET-1 gene, the homozygous adenine deletionwas associated to normal plasma levels of glutamate/pyruvate transaminase enzyme activity, uricacid concentration, cholesterol, and Low Density Lipoprotein in hypertensive subjects withoutclinical risk.Conclusion: We observed a gender-specific protective effect for EDNRA gene variations, thesubjects that carried the TT genotype presented more aggressive symptomatology. These resultsshow an association between plasmatic biochemical parameters, the clinical condition, andpolymorphisms in the endothelin axis genes.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23235
Lassen, Oscar; Herrera, Jimena María; Dotto, Gladys; Ojeda, Silvia; Garutti, Alicia; et al.; Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study; Sciencedomain; British Journal of Medicine and Medical Research; 11; 7; 1-2016; 1-8
2231-0614
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23235
identifier_str_mv Lassen, Oscar; Herrera, Jimena María; Dotto, Gladys; Ojeda, Silvia; Garutti, Alicia; et al.; Plasmatic Biochemical Variables Associated with Polymorphisms in the Endothelin-1 and Endothelin-1 Receptor a Genes in Hypertensive Patients: Pilot Study; Sciencedomain; British Journal of Medicine and Medical Research; 11; 7; 1-2016; 1-8
2231-0614
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/11732
info:eu-repo/semantics/altIdentifier/doi/10.9734/BJMMR/2016/20520
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sciencedomain
publisher.none.fl_str_mv Sciencedomain
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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