Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive...

Autores: Cassinotti, Luis Roberto; Guil, María J.; Schöller, Mercedes Itati; Navarro, Mónica Paula; Bianciotti, Liliana Graciela; Vatta, Marcelo Sergio
Año de publicación: 2018
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Overactivity of the sympathetic nervous system and central endothelins (ETs) are involved in the development of hypertension. Besides the well-known brain structures involved in the regulation of blood pressure like the hypothalamus or locus coeruleus, evidence suggests that the olfactory bulb (OB) also modulates cardiovascular function. In the present study, we evaluated the interaction between the endothelinergic and catecholaminergic systems in the OB of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Following brain ET receptor type A (ETA) blockade by BQ610 (selective antagonist), transcriptional, traductional, and post-traductional changes in tyrosine hydroxylase (TH) were assessed in the OB of normotensive and DOCA-salt hypertensive rats. Time course variations in systolic blood pressure and heart rate were also registered. Results showed that ETA blockade dose dependently reduced blood pressure in hypertensive rats, but it did not change heart rate. It also prevented the increase in TH activity and expression (mRNA and protein) in the right OB of hypertensive animals. However, ETA blockade did not affect hemodynamics or TH in normotensive animals. Present results support that brain ETA are not involved in blood pressure regulation in normal rats, but they significantly contribute to chronic blood pressure elevation in hypertensive animals. Changes in TH activity and expression were observed in the right but not in the left OB, supporting functional asymmetry, in line with previous studies regarding cardiovascular regulation. Present findings provide further evidence on the role of ETs in the regulation of catecholaminergic activity and the contribution of the right OB to DOCA-salt hypertension.
Fil: Cassinotti, Luis Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Guil, María J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Schöller, Mercedes Itati. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Navarro, Mónica Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Bianciotti, Liliana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Vatta, Marcelo Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia: BQ610
ENDOTHELIN
HYPERTENSION
SYSTOLIC BLOOD PRESSURE
TYROSINE HYDROXYLASE
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/87013

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network_name_str	CONICET Digital (CONICET)
spelling	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive ratsCassinotti, Luis RobertoGuil, María J.Schöller, Mercedes ItatiNavarro, Mónica PaulaBianciotti, Liliana GracielaVatta, Marcelo SergioBQ610ENDOTHELINHYPERTENSIONSYSTOLIC BLOOD PRESSURETYROSINE HYDROXYLASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Overactivity of the sympathetic nervous system and central endothelins (ETs) are involved in the development of hypertension. Besides the well-known brain structures involved in the regulation of blood pressure like the hypothalamus or locus coeruleus, evidence suggests that the olfactory bulb (OB) also modulates cardiovascular function. In the present study, we evaluated the interaction between the endothelinergic and catecholaminergic systems in the OB of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Following brain ET receptor type A (ETA) blockade by BQ610 (selective antagonist), transcriptional, traductional, and post-traductional changes in tyrosine hydroxylase (TH) were assessed in the OB of normotensive and DOCA-salt hypertensive rats. Time course variations in systolic blood pressure and heart rate were also registered. Results showed that ETA blockade dose dependently reduced blood pressure in hypertensive rats, but it did not change heart rate. It also prevented the increase in TH activity and expression (mRNA and protein) in the right OB of hypertensive animals. However, ETA blockade did not affect hemodynamics or TH in normotensive animals. Present results support that brain ETA are not involved in blood pressure regulation in normal rats, but they significantly contribute to chronic blood pressure elevation in hypertensive animals. Changes in TH activity and expression were observed in the right but not in the left OB, supporting functional asymmetry, in line with previous studies regarding cardiovascular regulation. Present findings provide further evidence on the role of ETs in the regulation of catecholaminergic activity and the contribution of the right OB to DOCA-salt hypertension.Fil: Cassinotti, Luis Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Guil, María J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Schöller, Mercedes Itati. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Navarro, Mónica Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Bianciotti, Liliana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Vatta, Marcelo Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaMolecular Diversity Preservation International2018-03-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87013Cassinotti, Luis Roberto; Guil, María J.; Schöller, Mercedes Itati; Navarro, Mónica Paula; Bianciotti, Liliana Graciela; et al.; Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 19; 3; 8-3-2018; 660-6781422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/19/3/660info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms19030660info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:59Zoai:ri.conicet.gov.ar:11336/87013instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:59.31CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
title	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
spellingShingle	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats Cassinotti, Luis Roberto BQ610 ENDOTHELIN HYPERTENSION SYSTOLIC BLOOD PRESSURE TYROSINE HYDROXYLASE
title_short	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
title_full	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
title_fullStr	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
title_full_unstemmed	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
title_sort	Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats
dc.creator.none.fl_str_mv	Cassinotti, Luis Roberto Guil, María J. Schöller, Mercedes Itati Navarro, Mónica Paula Bianciotti, Liliana Graciela Vatta, Marcelo Sergio
author	Cassinotti, Luis Roberto
author_facet	Cassinotti, Luis Roberto Guil, María J. Schöller, Mercedes Itati Navarro, Mónica Paula Bianciotti, Liliana Graciela Vatta, Marcelo Sergio
author_role	author
author2	Guil, María J. Schöller, Mercedes Itati Navarro, Mónica Paula Bianciotti, Liliana Graciela Vatta, Marcelo Sergio
author2_role	author author author author author
dc.subject.none.fl_str_mv	BQ610 ENDOTHELIN HYPERTENSION SYSTOLIC BLOOD PRESSURE TYROSINE HYDROXYLASE
topic	BQ610 ENDOTHELIN HYPERTENSION SYSTOLIC BLOOD PRESSURE TYROSINE HYDROXYLASE
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	Overactivity of the sympathetic nervous system and central endothelins (ETs) are involved in the development of hypertension. Besides the well-known brain structures involved in the regulation of blood pressure like the hypothalamus or locus coeruleus, evidence suggests that the olfactory bulb (OB) also modulates cardiovascular function. In the present study, we evaluated the interaction between the endothelinergic and catecholaminergic systems in the OB of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Following brain ET receptor type A (ETA) blockade by BQ610 (selective antagonist), transcriptional, traductional, and post-traductional changes in tyrosine hydroxylase (TH) were assessed in the OB of normotensive and DOCA-salt hypertensive rats. Time course variations in systolic blood pressure and heart rate were also registered. Results showed that ETA blockade dose dependently reduced blood pressure in hypertensive rats, but it did not change heart rate. It also prevented the increase in TH activity and expression (mRNA and protein) in the right OB of hypertensive animals. However, ETA blockade did not affect hemodynamics or TH in normotensive animals. Present results support that brain ETA are not involved in blood pressure regulation in normal rats, but they significantly contribute to chronic blood pressure elevation in hypertensive animals. Changes in TH activity and expression were observed in the right but not in the left OB, supporting functional asymmetry, in line with previous studies regarding cardiovascular regulation. Present findings provide further evidence on the role of ETs in the regulation of catecholaminergic activity and the contribution of the right OB to DOCA-salt hypertension. Fil: Cassinotti, Luis Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Guil, María J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Schöller, Mercedes Itati. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Navarro, Mónica Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Bianciotti, Liliana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Vatta, Marcelo Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description	Overactivity of the sympathetic nervous system and central endothelins (ETs) are involved in the development of hypertension. Besides the well-known brain structures involved in the regulation of blood pressure like the hypothalamus or locus coeruleus, evidence suggests that the olfactory bulb (OB) also modulates cardiovascular function. In the present study, we evaluated the interaction between the endothelinergic and catecholaminergic systems in the OB of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Following brain ET receptor type A (ETA) blockade by BQ610 (selective antagonist), transcriptional, traductional, and post-traductional changes in tyrosine hydroxylase (TH) were assessed in the OB of normotensive and DOCA-salt hypertensive rats. Time course variations in systolic blood pressure and heart rate were also registered. Results showed that ETA blockade dose dependently reduced blood pressure in hypertensive rats, but it did not change heart rate. It also prevented the increase in TH activity and expression (mRNA and protein) in the right OB of hypertensive animals. However, ETA blockade did not affect hemodynamics or TH in normotensive animals. Present results support that brain ETA are not involved in blood pressure regulation in normal rats, but they significantly contribute to chronic blood pressure elevation in hypertensive animals. Changes in TH activity and expression were observed in the right but not in the left OB, supporting functional asymmetry, in line with previous studies regarding cardiovascular regulation. Present findings provide further evidence on the role of ETs in the regulation of catecholaminergic activity and the contribution of the right OB to DOCA-salt hypertension.
publishDate	2018
dc.date.none.fl_str_mv	2018-03-08
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/87013 Cassinotti, Luis Roberto; Guil, María J.; Schöller, Mercedes Itati; Navarro, Mónica Paula; Bianciotti, Liliana Graciela; et al.; Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 19; 3; 8-3-2018; 660-678 1422-0067 CONICET Digital CONICET
url	http://hdl.handle.net/11336/87013
identifier_str_mv	Cassinotti, Luis Roberto; Guil, María J.; Schöller, Mercedes Itati; Navarro, Mónica Paula; Bianciotti, Liliana Graciela; et al.; Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive rats; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 19; 3; 8-3-2018; 660-678 1422-0067 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/19/3/660 info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms19030660
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv	Molecular Diversity Preservation International
publisher.none.fl_str_mv	Molecular Diversity Preservation International
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Chronic blockade of brain endothelin receptor type-a (ETA) reduces blood pressure and prevents catecholaminergic overactivity in the right olfactory bulb of DOCA-salt hypertensive...

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