Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors

Autores
Oka, Gabriel U.; Souza, Diorge P.; Sgro, Germán Gustavo; Guzzo, Cristiane R.; Dunger, Ricardo German; Farah, Chuck S.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many bacteria kill rival species by translocating toxic effectors intotarget cells. Effectors are often encoded along with cognateimmunity proteins that could (i) protect against“friendly-fire”(trans-intoxication) from neighboring sister cells and/or (ii) pro-tect against internalcis-intoxication (suicide). Here, we distinguishbetween these two mechanisms in the case of the bactericidalXanthomonas citriType IV Secretion System (X-T4SS). We use aset ofX. citrimutants lacking multiple effector/immunity protein(X-Tfe/X-Tfi) pairs to show that X-Tfis are not absolutely requiredto protect againsttrans-intoxication by wild-type cells. Our inves-tigation then focused on the in vivo function of the lysozyme-likeeffector X-TfeXAC2609and its cognate immunity protein X-TfiXAC2610.In the absence of X-TfiXAC2610, we observe X-TfeXAC2609-dependentand X-T4SS-independent accumulation of damage in theX. citricellenvelope, cell death, and inhibition of biofilm formation. Whileimmunity proteins in other systems have been shown to protectagainst attacks by sister cells (trans-intoxication), this is anexample of an antibacterial secretion system in which the immunityproteins are dedicated to protecting cells againstcis-intoxication.
Fil: Oka, Gabriel U.. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Souza, Diorge P.. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Guzzo, Cristiane R.. Universidade de Sao Paulo; Brasil
Fil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Farah, Chuck S.. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Materia
TYPE IV SECRETION SYSTEM
CIS-INTOXICATION
BIOFILM
IMMUNITY PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260583
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/260583
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectorsOka, Gabriel U.Souza, Diorge P.Sgro, Germán GustavoGuzzo, Cristiane R.Dunger, Ricardo GermanFarah, Chuck S.TYPE IV SECRETION SYSTEMCIS-INTOXICATIONBIOFILMIMMUNITY PROTEINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many bacteria kill rival species by translocating toxic effectors intotarget cells. Effectors are often encoded along with cognateimmunity proteins that could (i) protect against“friendly-fire”(trans-intoxication) from neighboring sister cells and/or (ii) pro-tect against internalcis-intoxication (suicide). Here, we distinguishbetween these two mechanisms in the case of the bactericidalXanthomonas citriType IV Secretion System (X-T4SS). We use aset ofX. citrimutants lacking multiple effector/immunity protein(X-Tfe/X-Tfi) pairs to show that X-Tfis are not absolutely requiredto protect againsttrans-intoxication by wild-type cells. Our inves-tigation then focused on the in vivo function of the lysozyme-likeeffector X-TfeXAC2609and its cognate immunity protein X-TfiXAC2610.In the absence of X-TfiXAC2610, we observe X-TfeXAC2609-dependentand X-T4SS-independent accumulation of damage in theX. citricellenvelope, cell death, and inhibition of biofilm formation. Whileimmunity proteins in other systems have been shown to protectagainst attacks by sister cells (trans-intoxication), this is anexample of an antibacterial secretion system in which the immunityproteins are dedicated to protecting cells againstcis-intoxication.Fil: Oka, Gabriel U.. Universidade do Sao Paulo. Departamento de Bioquímica; BrasilFil: Souza, Diorge P.. Universidade do Sao Paulo. Departamento de Bioquímica; BrasilFil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidade do Sao Paulo. Departamento de Bioquímica; BrasilFil: Guzzo, Cristiane R.. Universidade de Sao Paulo; BrasilFil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina. Universidade do Sao Paulo. Departamento de Bioquímica; BrasilFil: Farah, Chuck S.. Universidade do Sao Paulo. Departamento de Bioquímica; BrasilEMBO Press2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260583Oka, Gabriel U.; Souza, Diorge P.; Sgro, Germán Gustavo; Guzzo, Cristiane R.; Dunger, Ricardo German; et al.; Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors; EMBO Press; EMBO Reports; 25; 3; 2-2024; 1436-14521469-3178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.embopress.org/doi/full/10.1038/s44319-024-00060-6info:eu-repo/semantics/altIdentifier/doi/10.1038/s44319-024-00060-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:17Zoai:ri.conicet.gov.ar:11336/260583instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:17.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
title Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
spellingShingle Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
Oka, Gabriel U.
TYPE IV SECRETION SYSTEM
CIS-INTOXICATION
BIOFILM
IMMUNITY PROTEIN
title_short Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
title_full Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
title_fullStr Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
title_full_unstemmed Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
title_sort Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors
dc.creator.none.fl_str_mv Oka, Gabriel U.
Souza, Diorge P.
Sgro, Germán Gustavo
Guzzo, Cristiane R.
Dunger, Ricardo German
Farah, Chuck S.
author Oka, Gabriel U.
author_facet Oka, Gabriel U.
Souza, Diorge P.
Sgro, Germán Gustavo
Guzzo, Cristiane R.
Dunger, Ricardo German
Farah, Chuck S.
author_role author
author2 Souza, Diorge P.
Sgro, Germán Gustavo
Guzzo, Cristiane R.
Dunger, Ricardo German
Farah, Chuck S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv TYPE IV SECRETION SYSTEM
CIS-INTOXICATION
BIOFILM
IMMUNITY PROTEIN
topic TYPE IV SECRETION SYSTEM
CIS-INTOXICATION
BIOFILM
IMMUNITY PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many bacteria kill rival species by translocating toxic effectors intotarget cells. Effectors are often encoded along with cognateimmunity proteins that could (i) protect against“friendly-fire”(trans-intoxication) from neighboring sister cells and/or (ii) pro-tect against internalcis-intoxication (suicide). Here, we distinguishbetween these two mechanisms in the case of the bactericidalXanthomonas citriType IV Secretion System (X-T4SS). We use aset ofX. citrimutants lacking multiple effector/immunity protein(X-Tfe/X-Tfi) pairs to show that X-Tfis are not absolutely requiredto protect againsttrans-intoxication by wild-type cells. Our inves-tigation then focused on the in vivo function of the lysozyme-likeeffector X-TfeXAC2609and its cognate immunity protein X-TfiXAC2610.In the absence of X-TfiXAC2610, we observe X-TfeXAC2609-dependentand X-T4SS-independent accumulation of damage in theX. citricellenvelope, cell death, and inhibition of biofilm formation. Whileimmunity proteins in other systems have been shown to protectagainst attacks by sister cells (trans-intoxication), this is anexample of an antibacterial secretion system in which the immunityproteins are dedicated to protecting cells againstcis-intoxication.
Fil: Oka, Gabriel U.. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Souza, Diorge P.. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Guzzo, Cristiane R.. Universidade de Sao Paulo; Brasil
Fil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
Fil: Farah, Chuck S.. Universidade do Sao Paulo. Departamento de Bioquímica; Brasil
description Many bacteria kill rival species by translocating toxic effectors intotarget cells. Effectors are often encoded along with cognateimmunity proteins that could (i) protect against“friendly-fire”(trans-intoxication) from neighboring sister cells and/or (ii) pro-tect against internalcis-intoxication (suicide). Here, we distinguishbetween these two mechanisms in the case of the bactericidalXanthomonas citriType IV Secretion System (X-T4SS). We use aset ofX. citrimutants lacking multiple effector/immunity protein(X-Tfe/X-Tfi) pairs to show that X-Tfis are not absolutely requiredto protect againsttrans-intoxication by wild-type cells. Our inves-tigation then focused on the in vivo function of the lysozyme-likeeffector X-TfeXAC2609and its cognate immunity protein X-TfiXAC2610.In the absence of X-TfiXAC2610, we observe X-TfeXAC2609-dependentand X-T4SS-independent accumulation of damage in theX. citricellenvelope, cell death, and inhibition of biofilm formation. Whileimmunity proteins in other systems have been shown to protectagainst attacks by sister cells (trans-intoxication), this is anexample of an antibacterial secretion system in which the immunityproteins are dedicated to protecting cells againstcis-intoxication.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260583
Oka, Gabriel U.; Souza, Diorge P.; Sgro, Germán Gustavo; Guzzo, Cristiane R.; Dunger, Ricardo German; et al.; Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors; EMBO Press; EMBO Reports; 25; 3; 2-2024; 1436-1452
1469-3178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260583
identifier_str_mv Oka, Gabriel U.; Souza, Diorge P.; Sgro, Germán Gustavo; Guzzo, Cristiane R.; Dunger, Ricardo German; et al.; Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors; EMBO Press; EMBO Reports; 25; 3; 2-2024; 1436-1452
1469-3178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.embopress.org/doi/full/10.1038/s44319-024-00060-6
info:eu-repo/semantics/altIdentifier/doi/10.1038/s44319-024-00060-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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