25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties

Autores
Domingues, Marco M.; Gomes, Bárbara; Hollmann, Axel; Santos, Nuno C.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25‐hydroxycholesterol. The presence of 25‐hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25‐hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25‐ hydroxycholesterol inhibits the lipid‐condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25‐hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25‐hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)‐enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.
Fil: Domingues, Marco M.. Universidade Nova de Lisboa; Portugal
Fil: Gomes, Bárbara. Universidade Nova de Lisboa; Portugal
Fil: Hollmann, Axel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Santos, Nuno C.. Universidade Nova de Lisboa; Portugal
Materia
25‐HYDROXYCHOLESTEROL
ATOMIC FORCE MICROSCOPY
CHOLESTEROL
FORCE SPECTROSCOPY
SUPPORTED LIPID BILAYERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184309
Acceder
id CONICETDig_9361f217eab82be82dba174ffba07965
oai_identifier_str oai:ri.conicet.gov.ar:11336/184309
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 25-Hydroxycholesterol Effect on Membrane Structure andMechanical PropertiesDomingues, Marco M.Gomes, BárbaraHollmann, AxelSantos, Nuno C.25‐HYDROXYCHOLESTEROLATOMIC FORCE MICROSCOPYCHOLESTEROLFORCE SPECTROSCOPYSUPPORTED LIPID BILAYERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25‐hydroxycholesterol. The presence of 25‐hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25‐hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25‐ hydroxycholesterol inhibits the lipid‐condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25‐hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25‐hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)‐enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.Fil: Domingues, Marco M.. Universidade Nova de Lisboa; PortugalFil: Gomes, Bárbara. Universidade Nova de Lisboa; PortugalFil: Hollmann, Axel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; ArgentinaFil: Santos, Nuno C.. Universidade Nova de Lisboa; PortugalMDPI AG2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184309Domingues, Marco M.; Gomes, Bárbara; Hollmann, Axel; Santos, Nuno C.; 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties; MDPI AG; International Journal of Molecular Sciences; 22; 5; 3-2021; 1-121422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/5/2574info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms22052574info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:41Zoai:ri.conicet.gov.ar:11336/184309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:41.778CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
title 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
spellingShingle 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
Domingues, Marco M.
25‐HYDROXYCHOLESTEROL
ATOMIC FORCE MICROSCOPY
CHOLESTEROL
FORCE SPECTROSCOPY
SUPPORTED LIPID BILAYERS
title_short 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
title_full 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
title_fullStr 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
title_full_unstemmed 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
title_sort 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties
dc.creator.none.fl_str_mv Domingues, Marco M.
Gomes, Bárbara
Hollmann, Axel
Santos, Nuno C.
author Domingues, Marco M.
author_facet Domingues, Marco M.
Gomes, Bárbara
Hollmann, Axel
Santos, Nuno C.
author_role author
author2 Gomes, Bárbara
Hollmann, Axel
Santos, Nuno C.
author2_role author
author
author
dc.subject.none.fl_str_mv 25‐HYDROXYCHOLESTEROL
ATOMIC FORCE MICROSCOPY
CHOLESTEROL
FORCE SPECTROSCOPY
SUPPORTED LIPID BILAYERS
topic 25‐HYDROXYCHOLESTEROL
ATOMIC FORCE MICROSCOPY
CHOLESTEROL
FORCE SPECTROSCOPY
SUPPORTED LIPID BILAYERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25‐hydroxycholesterol. The presence of 25‐hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25‐hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25‐ hydroxycholesterol inhibits the lipid‐condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25‐hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25‐hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)‐enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.
Fil: Domingues, Marco M.. Universidade Nova de Lisboa; Portugal
Fil: Gomes, Bárbara. Universidade Nova de Lisboa; Portugal
Fil: Hollmann, Axel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Santos, Nuno C.. Universidade Nova de Lisboa; Portugal
description Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25‐hydroxycholesterol. The presence of 25‐hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25‐hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25‐ hydroxycholesterol inhibits the lipid‐condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25‐hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25‐hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)‐enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184309
Domingues, Marco M.; Gomes, Bárbara; Hollmann, Axel; Santos, Nuno C.; 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties; MDPI AG; International Journal of Molecular Sciences; 22; 5; 3-2021; 1-12
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184309
identifier_str_mv Domingues, Marco M.; Gomes, Bárbara; Hollmann, Axel; Santos, Nuno C.; 25-Hydroxycholesterol Effect on Membrane Structure andMechanical Properties; MDPI AG; International Journal of Molecular Sciences; 22; 5; 3-2021; 1-12
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/5/2574
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms22052574
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614447928180736
score 13.070432

Publicaciones similares