Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains

Autores
Vazquez, Romina Florencia; Ovalle García, Erasmo; Antillón, Armando; Ortega Blake, Iván; Muñoz Garay, Carlos; Maté, Sabina María
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.
Fil: Vazquez, Romina Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Ovalle García, Erasmo. Universidad Nacional Autónoma de México; México
Fil: Antillón, Armando. Universidad Nacional Autónoma de México; México
Fil: Ortega Blake, Iván. Universidad Nacional Autónoma de México; México
Fil: Muñoz Garay, Carlos. Universidad Nacional Autónoma de México; México
Fil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Materia
ATOMIC FORCE MICROSCOPY-FORCE SPECTROSCOPY
CYCLODEXTRINS
LIPID DOMAINS
LIPID EXCHANGE
MEMBRANE ASYMMETRY
SUPPORTED LIPID BILAYERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214559
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domainsVazquez, Romina FlorenciaOvalle García, ErasmoAntillón, ArmandoOrtega Blake, IvánMuñoz Garay, CarlosMaté, Sabina MaríaATOMIC FORCE MICROSCOPY-FORCE SPECTROSCOPYCYCLODEXTRINSLIPID DOMAINSLIPID EXCHANGEMEMBRANE ASYMMETRYSUPPORTED LIPID BILAYERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.Fil: Vazquez, Romina Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Ovalle García, Erasmo. Universidad Nacional Autónoma de México; MéxicoFil: Antillón, Armando. Universidad Nacional Autónoma de México; MéxicoFil: Ortega Blake, Iván. Universidad Nacional Autónoma de México; MéxicoFil: Muñoz Garay, Carlos. Universidad Nacional Autónoma de México; MéxicoFil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaHumana Press2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214559Vazquez, Romina Florencia; Ovalle García, Erasmo; Antillón, Armando; Ortega Blake, Iván; Muñoz Garay, Carlos; et al.; Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains; Humana Press; Methods in Molecular Biology; 2402; 1-2022; 243-256978-1-0716-1842-41064-3745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-0716-1843-1_19info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-0716-1843-1_19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:05Zoai:ri.conicet.gov.ar:11336/214559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:05.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
title Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
spellingShingle Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
Vazquez, Romina Florencia
ATOMIC FORCE MICROSCOPY-FORCE SPECTROSCOPY
CYCLODEXTRINS
LIPID DOMAINS
LIPID EXCHANGE
MEMBRANE ASYMMETRY
SUPPORTED LIPID BILAYERS
title_short Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
title_full Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
title_fullStr Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
title_full_unstemmed Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
title_sort Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
dc.creator.none.fl_str_mv Vazquez, Romina Florencia
Ovalle García, Erasmo
Antillón, Armando
Ortega Blake, Iván
Muñoz Garay, Carlos
Maté, Sabina María
author Vazquez, Romina Florencia
author_facet Vazquez, Romina Florencia
Ovalle García, Erasmo
Antillón, Armando
Ortega Blake, Iván
Muñoz Garay, Carlos
Maté, Sabina María
author_role author
author2 Ovalle García, Erasmo
Antillón, Armando
Ortega Blake, Iván
Muñoz Garay, Carlos
Maté, Sabina María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ATOMIC FORCE MICROSCOPY-FORCE SPECTROSCOPY
CYCLODEXTRINS
LIPID DOMAINS
LIPID EXCHANGE
MEMBRANE ASYMMETRY
SUPPORTED LIPID BILAYERS
topic ATOMIC FORCE MICROSCOPY-FORCE SPECTROSCOPY
CYCLODEXTRINS
LIPID DOMAINS
LIPID EXCHANGE
MEMBRANE ASYMMETRY
SUPPORTED LIPID BILAYERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.
Fil: Vazquez, Romina Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Ovalle García, Erasmo. Universidad Nacional Autónoma de México; México
Fil: Antillón, Armando. Universidad Nacional Autónoma de México; México
Fil: Ortega Blake, Iván. Universidad Nacional Autónoma de México; México
Fil: Muñoz Garay, Carlos. Universidad Nacional Autónoma de México; México
Fil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
description The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214559
Vazquez, Romina Florencia; Ovalle García, Erasmo; Antillón, Armando; Ortega Blake, Iván; Muñoz Garay, Carlos; et al.; Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains; Humana Press; Methods in Molecular Biology; 2402; 1-2022; 243-256
978-1-0716-1842-4
1064-3745
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214559
identifier_str_mv Vazquez, Romina Florencia; Ovalle García, Erasmo; Antillón, Armando; Ortega Blake, Iván; Muñoz Garay, Carlos; et al.; Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains; Humana Press; Methods in Molecular Biology; 2402; 1-2022; 243-256
978-1-0716-1842-4
1064-3745
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-0716-1843-1_19
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-0716-1843-1_19
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614356865646592
score 13.070432

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