Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis

Autores
Principe, Gabriel; Lezcano, Virginia Alicia; Tiburzi, Silvina Mabel; Miravalles, Alicia Beatriz; Montiel Schneider, María Gabriela; Lassalle, Verónica Leticia; González Pardo, María Verónica
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Magnetic nanotheranostics represent promising alternatives to the traditional diagnostic and treatment procedures available for different pathologies. The goal of this work was to analyze the biological action of polyethylene glycol-coated iron oxide nanoparticles (MAG.PEG) to generate a non-toxic carrier to optimize the delivery of drugs for Kaposi?s sarcoma treatment. The MAG.PEG were synthesized by the hydrothermal method displaying a hydrodynamic diameter of 204 nm and a zeta potential of -22.1 mV. Firstly, MAG.PEG effects on cytotoxicity and cell viability were evaluated on endothelial cells expressing vGPCR, which prompts Kaposi?s sarcoma. By Trypan blue technique, we found that the incubation of these cells neither with MAG nor MAG.PEG (1-150 μg/ml) provoked significant changes in the number of living cells. In addition, cell viability analyzes (MTS and neutral red) revealed that a significant increase in metabolic and lysosomal activity was detected at higher concentrations of MAG or MAG.PEG (100-150 μg/ml) after 48 h of incubation. Secondly, the localization and accumulation of MAG.PEG (1-150 μg/ml) towards the cells was observed directly under a phase contrast microscope. In addition, MAG.PEG assembled within or nearby the cells and cell morphology remained unchanged regardless of the nanoparticles concentration. Furthermore, the presence of vesicles containing MAG.PEG inside vGPCR cells was confirmed by transmission electron microscopy. Thirdly, the iron content quantified by Prussian blue staining showed that the degree of accumulation of MAG.PEG depends on the concentration used. Finally, to steer the MAG.PEG to certain localization in the cell culture, a magnetic field generated by a moderated power magnet was used. The results indicated that the magnetic stimuli induced MAG.PEG accumulation in the zone where the magnet was placed. In conclusion, concentrations between 1 and 50 μg/ml of MAG.PEG would be suitable as drug carriers in this cellular model since no alterations in cell proliferation and viability were observed under the tested conditions. In addition, targeting MAG.PEG to a tumor with a magnet would avoid adverse effects on normal tissues.
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Montiel Schneider, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
NANOTECHNOLOGY
DRUG CARRIER
PEGYLATION
Fe3O4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228657
Acceder
id CONICETDig_9349e3b6de009756f07b422b29593139
oai_identifier_str oai:ri.conicet.gov.ar:11336/228657
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesisPrincipe, GabrielLezcano, Virginia AliciaTiburzi, Silvina MabelMiravalles, Alicia BeatrizMontiel Schneider, María GabrielaLassalle, Verónica LeticiaGonzález Pardo, María VerónicaNANOTECHNOLOGYDRUG CARRIERPEGYLATIONFe3O4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Magnetic nanotheranostics represent promising alternatives to the traditional diagnostic and treatment procedures available for different pathologies. The goal of this work was to analyze the biological action of polyethylene glycol-coated iron oxide nanoparticles (MAG.PEG) to generate a non-toxic carrier to optimize the delivery of drugs for Kaposi?s sarcoma treatment. The MAG.PEG were synthesized by the hydrothermal method displaying a hydrodynamic diameter of 204 nm and a zeta potential of -22.1 mV. Firstly, MAG.PEG effects on cytotoxicity and cell viability were evaluated on endothelial cells expressing vGPCR, which prompts Kaposi?s sarcoma. By Trypan blue technique, we found that the incubation of these cells neither with MAG nor MAG.PEG (1-150 μg/ml) provoked significant changes in the number of living cells. In addition, cell viability analyzes (MTS and neutral red) revealed that a significant increase in metabolic and lysosomal activity was detected at higher concentrations of MAG or MAG.PEG (100-150 μg/ml) after 48 h of incubation. Secondly, the localization and accumulation of MAG.PEG (1-150 μg/ml) towards the cells was observed directly under a phase contrast microscope. In addition, MAG.PEG assembled within or nearby the cells and cell morphology remained unchanged regardless of the nanoparticles concentration. Furthermore, the presence of vesicles containing MAG.PEG inside vGPCR cells was confirmed by transmission electron microscopy. Thirdly, the iron content quantified by Prussian blue staining showed that the degree of accumulation of MAG.PEG depends on the concentration used. Finally, to steer the MAG.PEG to certain localization in the cell culture, a magnetic field generated by a moderated power magnet was used. The results indicated that the magnetic stimuli induced MAG.PEG accumulation in the zone where the magnet was placed. In conclusion, concentrations between 1 and 50 μg/ml of MAG.PEG would be suitable as drug carriers in this cellular model since no alterations in cell proliferation and viability were observed under the tested conditions. In addition, targeting MAG.PEG to a tumor with a magnet would avoid adverse effects on normal tissues.Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Montiel Schneider, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)MendozaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science PressCeccarelli, Eduardo Augusto2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228657Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Mendoza; Argentina; 2022; 1-21667-5746CONICET DigitalCONICETenghttps://newsite.saib.org.ar/congreso2022/info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:15Zoai:ri.conicet.gov.ar:11336/228657instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:15.695CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
title Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
spellingShingle Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
Principe, Gabriel
NANOTECHNOLOGY
DRUG CARRIER
PEGYLATION
Fe3O4
title_short Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
title_full Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
title_fullStr Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
title_full_unstemmed Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
title_sort Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis
dc.creator.none.fl_str_mv Principe, Gabriel
Lezcano, Virginia Alicia
Tiburzi, Silvina Mabel
Miravalles, Alicia Beatriz
Montiel Schneider, María Gabriela
Lassalle, Verónica Leticia
González Pardo, María Verónica
author Principe, Gabriel
author_facet Principe, Gabriel
Lezcano, Virginia Alicia
Tiburzi, Silvina Mabel
Miravalles, Alicia Beatriz
Montiel Schneider, María Gabriela
Lassalle, Verónica Leticia
González Pardo, María Verónica
author_role author
author2 Lezcano, Virginia Alicia
Tiburzi, Silvina Mabel
Miravalles, Alicia Beatriz
Montiel Schneider, María Gabriela
Lassalle, Verónica Leticia
González Pardo, María Verónica
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ceccarelli, Eduardo Augusto
dc.subject.none.fl_str_mv NANOTECHNOLOGY
DRUG CARRIER
PEGYLATION
Fe3O4
topic NANOTECHNOLOGY
DRUG CARRIER
PEGYLATION
Fe3O4
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Magnetic nanotheranostics represent promising alternatives to the traditional diagnostic and treatment procedures available for different pathologies. The goal of this work was to analyze the biological action of polyethylene glycol-coated iron oxide nanoparticles (MAG.PEG) to generate a non-toxic carrier to optimize the delivery of drugs for Kaposi?s sarcoma treatment. The MAG.PEG were synthesized by the hydrothermal method displaying a hydrodynamic diameter of 204 nm and a zeta potential of -22.1 mV. Firstly, MAG.PEG effects on cytotoxicity and cell viability were evaluated on endothelial cells expressing vGPCR, which prompts Kaposi?s sarcoma. By Trypan blue technique, we found that the incubation of these cells neither with MAG nor MAG.PEG (1-150 μg/ml) provoked significant changes in the number of living cells. In addition, cell viability analyzes (MTS and neutral red) revealed that a significant increase in metabolic and lysosomal activity was detected at higher concentrations of MAG or MAG.PEG (100-150 μg/ml) after 48 h of incubation. Secondly, the localization and accumulation of MAG.PEG (1-150 μg/ml) towards the cells was observed directly under a phase contrast microscope. In addition, MAG.PEG assembled within or nearby the cells and cell morphology remained unchanged regardless of the nanoparticles concentration. Furthermore, the presence of vesicles containing MAG.PEG inside vGPCR cells was confirmed by transmission electron microscopy. Thirdly, the iron content quantified by Prussian blue staining showed that the degree of accumulation of MAG.PEG depends on the concentration used. Finally, to steer the MAG.PEG to certain localization in the cell culture, a magnetic field generated by a moderated power magnet was used. The results indicated that the magnetic stimuli induced MAG.PEG accumulation in the zone where the magnet was placed. In conclusion, concentrations between 1 and 50 μg/ml of MAG.PEG would be suitable as drug carriers in this cellular model since no alterations in cell proliferation and viability were observed under the tested conditions. In addition, targeting MAG.PEG to a tumor with a magnet would avoid adverse effects on normal tissues.
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Montiel Schneider, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)
Mendoza
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Magnetic nanotheranostics represent promising alternatives to the traditional diagnostic and treatment procedures available for different pathologies. The goal of this work was to analyze the biological action of polyethylene glycol-coated iron oxide nanoparticles (MAG.PEG) to generate a non-toxic carrier to optimize the delivery of drugs for Kaposi?s sarcoma treatment. The MAG.PEG were synthesized by the hydrothermal method displaying a hydrodynamic diameter of 204 nm and a zeta potential of -22.1 mV. Firstly, MAG.PEG effects on cytotoxicity and cell viability were evaluated on endothelial cells expressing vGPCR, which prompts Kaposi?s sarcoma. By Trypan blue technique, we found that the incubation of these cells neither with MAG nor MAG.PEG (1-150 μg/ml) provoked significant changes in the number of living cells. In addition, cell viability analyzes (MTS and neutral red) revealed that a significant increase in metabolic and lysosomal activity was detected at higher concentrations of MAG or MAG.PEG (100-150 μg/ml) after 48 h of incubation. Secondly, the localization and accumulation of MAG.PEG (1-150 μg/ml) towards the cells was observed directly under a phase contrast microscope. In addition, MAG.PEG assembled within or nearby the cells and cell morphology remained unchanged regardless of the nanoparticles concentration. Furthermore, the presence of vesicles containing MAG.PEG inside vGPCR cells was confirmed by transmission electron microscopy. Thirdly, the iron content quantified by Prussian blue staining showed that the degree of accumulation of MAG.PEG depends on the concentration used. Finally, to steer the MAG.PEG to certain localization in the cell culture, a magnetic field generated by a moderated power magnet was used. The results indicated that the magnetic stimuli induced MAG.PEG accumulation in the zone where the magnet was placed. In conclusion, concentrations between 1 and 50 μg/ml of MAG.PEG would be suitable as drug carriers in this cellular model since no alterations in cell proliferation and viability were observed under the tested conditions. In addition, targeting MAG.PEG to a tumor with a magnet would avoid adverse effects on normal tissues.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228657
Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Mendoza; Argentina; 2022; 1-2
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228657
identifier_str_mv Characterization and biological action of polyethylene glycol-coated magnetite nanoparticles in a cellular model of viral oncogenesis; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Mendoza; Argentina; 2022; 1-2
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://newsite.saib.org.ar/congreso2022/
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v47nSuppl.1/50703
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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