Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion
- Autores
- Rodriguez, Lorena Gabriela; Di Venosa, Gabriela Mariana; Rivas, Martín A.; Juarranz, Angeles; Sanz Rodriguez, Francisco; Casas, Adriana Gabriela
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: Photodynamic therapy (PDT) is a treatment modality for several cancers involving the administration of a tumour-localising photosensitiser (PS) and its subsequent activation by light, resulting in tumour damage. Ras oncogenes have been strongly associated with chemo- and radio-resistance. Based on the described roles of adhesion and cell morphology on drug resistance, we studied if the differences in shape, cell-extracellular matrix and cell-cell adhesion induced by Ras transfection, play a role in the resistance to PDT. Materials and methods: We employed the human normal breast HB4a cells transfected with H-RAS and a panel of five PSs. Key findings: We found that resistance to PDT of the HB4a-Ras cells employing all the PSs, increased between 1.3 and 2.5-fold as compared to the parental cells. There was no correlation between resistance and intracellular PS levels or PS intracellular localisation. Even when Ras-transfected cells present lower adherence to the ECM proteins, this does not make them more sensitive to PDT or chemotherapy. On the contrary, a marked gain of resistance to PDT was observed in floating cells as compared to adhesive cells, accounting for the higher ability conferred by Ras to survive in conditions of decreased cell-extracellular matrix interactions. HB4a-Ras cells displayed disorganisation of actin fibres, mislocalised E-cadherin and vinculin and lower expression of E-cadherin and β1-integrin as compared to HB4a cells. Significance: Knowledge of the mechanisms of resistance to photodamage in Ras-overexpressing cells may lead to the optimization of the combination of PDT with other treatments.
Fil: Rodriguez, Lorena Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Rivas, Martín A.. Weill Cornell Medicine; Estados Unidos
Fil: Juarranz, Angeles. Universidad Autónoma de Madrid; España
Fil: Sanz Rodriguez, Francisco. Universidad Autónoma de Madrid; España
Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina - Materia
-
ACRIDINE ORANGE (PUBCHEM CID62344)
ADHESION
CHLORIN E6 (PUBCHEM CID5479494)
CISPLATIN (PUBCHEM CID5702198)
CYTOSKELETON
DOXORUBICIN (PUBCHEM CID31703)
FLUOROURACIL (PUBCHEM CID3385)
MEROCYANINE 540 (PUBCHEM CID5702743)
METHOTREXATE (PUBCHEM CID126941)
MITOMYCIN C (PUBCHEM CID5746)
PHOTODYNAMIC THERAPY
PORFIMER SODIUM (PUBCHEM CID136187588)
RAS ONCOGENE
RESISTANCE
TEMOPORFIN (PUBCHEM CID60751) - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/228158
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oai:ri.conicet.gov.ar:11336/228158 |
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CONICET Digital (CONICET) |
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Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesionRodriguez, Lorena GabrielaDi Venosa, Gabriela MarianaRivas, Martín A.Juarranz, AngelesSanz Rodriguez, FranciscoCasas, Adriana GabrielaACRIDINE ORANGE (PUBCHEM CID62344)ADHESIONCHLORIN E6 (PUBCHEM CID5479494)CISPLATIN (PUBCHEM CID5702198)CYTOSKELETONDOXORUBICIN (PUBCHEM CID31703)FLUOROURACIL (PUBCHEM CID3385)MEROCYANINE 540 (PUBCHEM CID5702743)METHOTREXATE (PUBCHEM CID126941)MITOMYCIN C (PUBCHEM CID5746)PHOTODYNAMIC THERAPYPORFIMER SODIUM (PUBCHEM CID136187588)RAS ONCOGENERESISTANCETEMOPORFIN (PUBCHEM CID60751)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: Photodynamic therapy (PDT) is a treatment modality for several cancers involving the administration of a tumour-localising photosensitiser (PS) and its subsequent activation by light, resulting in tumour damage. Ras oncogenes have been strongly associated with chemo- and radio-resistance. Based on the described roles of adhesion and cell morphology on drug resistance, we studied if the differences in shape, cell-extracellular matrix and cell-cell adhesion induced by Ras transfection, play a role in the resistance to PDT. Materials and methods: We employed the human normal breast HB4a cells transfected with H-RAS and a panel of five PSs. Key findings: We found that resistance to PDT of the HB4a-Ras cells employing all the PSs, increased between 1.3 and 2.5-fold as compared to the parental cells. There was no correlation between resistance and intracellular PS levels or PS intracellular localisation. Even when Ras-transfected cells present lower adherence to the ECM proteins, this does not make them more sensitive to PDT or chemotherapy. On the contrary, a marked gain of resistance to PDT was observed in floating cells as compared to adhesive cells, accounting for the higher ability conferred by Ras to survive in conditions of decreased cell-extracellular matrix interactions. HB4a-Ras cells displayed disorganisation of actin fibres, mislocalised E-cadherin and vinculin and lower expression of E-cadherin and β1-integrin as compared to HB4a cells. Significance: Knowledge of the mechanisms of resistance to photodamage in Ras-overexpressing cells may lead to the optimization of the combination of PDT with other treatments.Fil: Rodriguez, Lorena Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Rivas, Martín A.. Weill Cornell Medicine; Estados UnidosFil: Juarranz, Angeles. Universidad Autónoma de Madrid; EspañaFil: Sanz Rodriguez, Francisco. Universidad Autónoma de Madrid; EspañaFil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaPergamon-Elsevier Science Ltd2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228158Rodriguez, Lorena Gabriela; Di Venosa, Gabriela Mariana; Rivas, Martín A.; Juarranz, Angeles; Sanz Rodriguez, Francisco; et al.; Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion; Pergamon-Elsevier Science Ltd; Life Sciences; 314; 121287; 2-2023; 1-100024-3205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2022.121287info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0024320522009870info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:07Zoai:ri.conicet.gov.ar:11336/228158instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:08.017CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
title |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
spellingShingle |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion Rodriguez, Lorena Gabriela ACRIDINE ORANGE (PUBCHEM CID62344) ADHESION CHLORIN E6 (PUBCHEM CID5479494) CISPLATIN (PUBCHEM CID5702198) CYTOSKELETON DOXORUBICIN (PUBCHEM CID31703) FLUOROURACIL (PUBCHEM CID3385) MEROCYANINE 540 (PUBCHEM CID5702743) METHOTREXATE (PUBCHEM CID126941) MITOMYCIN C (PUBCHEM CID5746) PHOTODYNAMIC THERAPY PORFIMER SODIUM (PUBCHEM CID136187588) RAS ONCOGENE RESISTANCE TEMOPORFIN (PUBCHEM CID60751) |
title_short |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
title_full |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
title_fullStr |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
title_full_unstemmed |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
title_sort |
Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion |
dc.creator.none.fl_str_mv |
Rodriguez, Lorena Gabriela Di Venosa, Gabriela Mariana Rivas, Martín A. Juarranz, Angeles Sanz Rodriguez, Francisco Casas, Adriana Gabriela |
author |
Rodriguez, Lorena Gabriela |
author_facet |
Rodriguez, Lorena Gabriela Di Venosa, Gabriela Mariana Rivas, Martín A. Juarranz, Angeles Sanz Rodriguez, Francisco Casas, Adriana Gabriela |
author_role |
author |
author2 |
Di Venosa, Gabriela Mariana Rivas, Martín A. Juarranz, Angeles Sanz Rodriguez, Francisco Casas, Adriana Gabriela |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
ACRIDINE ORANGE (PUBCHEM CID62344) ADHESION CHLORIN E6 (PUBCHEM CID5479494) CISPLATIN (PUBCHEM CID5702198) CYTOSKELETON DOXORUBICIN (PUBCHEM CID31703) FLUOROURACIL (PUBCHEM CID3385) MEROCYANINE 540 (PUBCHEM CID5702743) METHOTREXATE (PUBCHEM CID126941) MITOMYCIN C (PUBCHEM CID5746) PHOTODYNAMIC THERAPY PORFIMER SODIUM (PUBCHEM CID136187588) RAS ONCOGENE RESISTANCE TEMOPORFIN (PUBCHEM CID60751) |
topic |
ACRIDINE ORANGE (PUBCHEM CID62344) ADHESION CHLORIN E6 (PUBCHEM CID5479494) CISPLATIN (PUBCHEM CID5702198) CYTOSKELETON DOXORUBICIN (PUBCHEM CID31703) FLUOROURACIL (PUBCHEM CID3385) MEROCYANINE 540 (PUBCHEM CID5702743) METHOTREXATE (PUBCHEM CID126941) MITOMYCIN C (PUBCHEM CID5746) PHOTODYNAMIC THERAPY PORFIMER SODIUM (PUBCHEM CID136187588) RAS ONCOGENE RESISTANCE TEMOPORFIN (PUBCHEM CID60751) |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Aims: Photodynamic therapy (PDT) is a treatment modality for several cancers involving the administration of a tumour-localising photosensitiser (PS) and its subsequent activation by light, resulting in tumour damage. Ras oncogenes have been strongly associated with chemo- and radio-resistance. Based on the described roles of adhesion and cell morphology on drug resistance, we studied if the differences in shape, cell-extracellular matrix and cell-cell adhesion induced by Ras transfection, play a role in the resistance to PDT. Materials and methods: We employed the human normal breast HB4a cells transfected with H-RAS and a panel of five PSs. Key findings: We found that resistance to PDT of the HB4a-Ras cells employing all the PSs, increased between 1.3 and 2.5-fold as compared to the parental cells. There was no correlation between resistance and intracellular PS levels or PS intracellular localisation. Even when Ras-transfected cells present lower adherence to the ECM proteins, this does not make them more sensitive to PDT or chemotherapy. On the contrary, a marked gain of resistance to PDT was observed in floating cells as compared to adhesive cells, accounting for the higher ability conferred by Ras to survive in conditions of decreased cell-extracellular matrix interactions. HB4a-Ras cells displayed disorganisation of actin fibres, mislocalised E-cadherin and vinculin and lower expression of E-cadherin and β1-integrin as compared to HB4a cells. Significance: Knowledge of the mechanisms of resistance to photodamage in Ras-overexpressing cells may lead to the optimization of the combination of PDT with other treatments. Fil: Rodriguez, Lorena Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina Fil: Rivas, Martín A.. Weill Cornell Medicine; Estados Unidos Fil: Juarranz, Angeles. Universidad Autónoma de Madrid; España Fil: Sanz Rodriguez, Francisco. Universidad Autónoma de Madrid; España Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina |
description |
Aims: Photodynamic therapy (PDT) is a treatment modality for several cancers involving the administration of a tumour-localising photosensitiser (PS) and its subsequent activation by light, resulting in tumour damage. Ras oncogenes have been strongly associated with chemo- and radio-resistance. Based on the described roles of adhesion and cell morphology on drug resistance, we studied if the differences in shape, cell-extracellular matrix and cell-cell adhesion induced by Ras transfection, play a role in the resistance to PDT. Materials and methods: We employed the human normal breast HB4a cells transfected with H-RAS and a panel of five PSs. Key findings: We found that resistance to PDT of the HB4a-Ras cells employing all the PSs, increased between 1.3 and 2.5-fold as compared to the parental cells. There was no correlation between resistance and intracellular PS levels or PS intracellular localisation. Even when Ras-transfected cells present lower adherence to the ECM proteins, this does not make them more sensitive to PDT or chemotherapy. On the contrary, a marked gain of resistance to PDT was observed in floating cells as compared to adhesive cells, accounting for the higher ability conferred by Ras to survive in conditions of decreased cell-extracellular matrix interactions. HB4a-Ras cells displayed disorganisation of actin fibres, mislocalised E-cadherin and vinculin and lower expression of E-cadherin and β1-integrin as compared to HB4a cells. Significance: Knowledge of the mechanisms of resistance to photodamage in Ras-overexpressing cells may lead to the optimization of the combination of PDT with other treatments. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/228158 Rodriguez, Lorena Gabriela; Di Venosa, Gabriela Mariana; Rivas, Martín A.; Juarranz, Angeles; Sanz Rodriguez, Francisco; et al.; Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion; Pergamon-Elsevier Science Ltd; Life Sciences; 314; 121287; 2-2023; 1-10 0024-3205 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/228158 |
identifier_str_mv |
Rodriguez, Lorena Gabriela; Di Venosa, Gabriela Mariana; Rivas, Martín A.; Juarranz, Angeles; Sanz Rodriguez, Francisco; et al.; Ras-transfected human mammary tumour cells are resistant to photodynamic therapy by mechanisms related to cell adhesion; Pergamon-Elsevier Science Ltd; Life Sciences; 314; 121287; 2-2023; 1-10 0024-3205 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2022.121287 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0024320522009870 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614249769336832 |
score |
13.070432 |