Isoform specificity of progesterone receptor antibodies

Autores
Fabris, Victoria Teresa; Abascal, Maria Florencia; Giulianelli, Sebastian Jesus; May, María; Sequeira, Gonzalo Ricardo; Jacobsen, Britta; Lombès, Marc; Han, Julie; Tran, Luan; Molinolo, Alfredo; Lanari, Claudia Lee Malvina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Progesterone receptors (PR) are prognostic and predictive biomarkers in hormone-dependent cancers. Twomain PR isoforms have been described, PRB and PRA, that differ only in that PRB has 164 extra N-terminalamino acids. It has been reported that several antibodies empirically exclusively recognize PRA in formalinfixedparaffin-embedded (FFPE) tissues. To confirm these findings, we used human breast cancer xenograftmodels, T47D-YA and -YB cells expressing PRA or PRB, respectively, MDA-MB-231 cells modified to synthesizePRB, and MDA-MB-231/iPRAB cells which can bi-inducibly express either PRA or PRB. Cells were injectedinto immunocompromised mice to generate tumours exclusively expressing PRA or PRB. PR isoform expressionwas verified using immunoblots. FFPE samples from the same tumours were studied by immunohistochemistryusing H-190, clone 636, clone 16, and Ab-6 anti-PR antibodies, the latter exclusively recognizing PRB. Exceptfor Ab-6, all antibodies displayed a similar staining pattern. Our results indicate that clones 16, 636, and theH-190 antibody recognize both PR isoforms. They point to the need for more stringency in evaluating thetrue specificity of purported PRA-specific antibodies as the PRA/PRB ratio may have prognostic and predictivevalue in breast cancer.
Fil: Fabris, Victoria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Abascal, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: May, María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Sequeira, Gonzalo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Jacobsen, Britta. University of Colorado Anschutz Medical Campus; Estados Unidos
Fil: Lombès, Marc. Inserm; Francia
Fil: Han, Julie. Moore´s Cancer Center; Estados Unidos
Fil: Tran, Luan. Moore´s Cancer Center; Estados Unidos
Fil: Molinolo, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
PROGESTERONE RECEPTOR ISOFORMS
BREAST CANCER MODELS; XENOGRAFTS
SPECIFIC ANTIBODIES
IMMUNOHISTOCHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37345
Acceder
id CONICETDig_932d04ecf510e2c5fcb5ad77a02e27b4
oai_identifier_str oai:ri.conicet.gov.ar:11336/37345
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isoform specificity of progesterone receptor antibodiesFabris, Victoria TeresaAbascal, Maria FlorenciaGiulianelli, Sebastian JesusMay, MaríaSequeira, Gonzalo RicardoJacobsen, BrittaLombès, MarcHan, JulieTran, LuanMolinolo, AlfredoLanari, Claudia Lee MalvinaPROGESTERONE RECEPTOR ISOFORMSBREAST CANCER MODELS; XENOGRAFTSSPECIFIC ANTIBODIESIMMUNOHISTOCHEMISTRYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Progesterone receptors (PR) are prognostic and predictive biomarkers in hormone-dependent cancers. Twomain PR isoforms have been described, PRB and PRA, that differ only in that PRB has 164 extra N-terminalamino acids. It has been reported that several antibodies empirically exclusively recognize PRA in formalinfixedparaffin-embedded (FFPE) tissues. To confirm these findings, we used human breast cancer xenograftmodels, T47D-YA and -YB cells expressing PRA or PRB, respectively, MDA-MB-231 cells modified to synthesizePRB, and MDA-MB-231/iPRAB cells which can bi-inducibly express either PRA or PRB. Cells were injectedinto immunocompromised mice to generate tumours exclusively expressing PRA or PRB. PR isoform expressionwas verified using immunoblots. FFPE samples from the same tumours were studied by immunohistochemistryusing H-190, clone 636, clone 16, and Ab-6 anti-PR antibodies, the latter exclusively recognizing PRB. Exceptfor Ab-6, all antibodies displayed a similar staining pattern. Our results indicate that clones 16, 636, and theH-190 antibody recognize both PR isoforms. They point to the need for more stringency in evaluating thetrue specificity of purported PRA-specific antibodies as the PRA/PRB ratio may have prognostic and predictivevalue in breast cancer.Fil: Fabris, Victoria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Abascal, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: May, María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Sequeira, Gonzalo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Jacobsen, Britta. University of Colorado Anschutz Medical Campus; Estados UnidosFil: Lombès, Marc. Inserm; FranciaFil: Han, Julie. Moore´s Cancer Center; Estados UnidosFil: Tran, Luan. Moore´s Cancer Center; Estados UnidosFil: Molinolo, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaWiley Online Library2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37345Fabris, Victoria Teresa; Abascal, Maria Florencia; Giulianelli, Sebastian Jesus; May, María; Sequeira, Gonzalo Ricardo; et al.; Isoform specificity of progesterone receptor antibodies; Wiley Online Library; The Journal of Pathology: Clinical Research; 3; 4; 10-2017; 227-2332056-4538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cjp2.83/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/cjp2.83info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:50Zoai:ri.conicet.gov.ar:11336/37345instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:51.248CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isoform specificity of progesterone receptor antibodies
title Isoform specificity of progesterone receptor antibodies
spellingShingle Isoform specificity of progesterone receptor antibodies
Fabris, Victoria Teresa
PROGESTERONE RECEPTOR ISOFORMS
BREAST CANCER MODELS; XENOGRAFTS
SPECIFIC ANTIBODIES
IMMUNOHISTOCHEMISTRY
title_short Isoform specificity of progesterone receptor antibodies
title_full Isoform specificity of progesterone receptor antibodies
title_fullStr Isoform specificity of progesterone receptor antibodies
title_full_unstemmed Isoform specificity of progesterone receptor antibodies
title_sort Isoform specificity of progesterone receptor antibodies
dc.creator.none.fl_str_mv Fabris, Victoria Teresa
Abascal, Maria Florencia
Giulianelli, Sebastian Jesus
May, María
Sequeira, Gonzalo Ricardo
Jacobsen, Britta
Lombès, Marc
Han, Julie
Tran, Luan
Molinolo, Alfredo
Lanari, Claudia Lee Malvina
author Fabris, Victoria Teresa
author_facet Fabris, Victoria Teresa
Abascal, Maria Florencia
Giulianelli, Sebastian Jesus
May, María
Sequeira, Gonzalo Ricardo
Jacobsen, Britta
Lombès, Marc
Han, Julie
Tran, Luan
Molinolo, Alfredo
Lanari, Claudia Lee Malvina
author_role author
author2 Abascal, Maria Florencia
Giulianelli, Sebastian Jesus
May, María
Sequeira, Gonzalo Ricardo
Jacobsen, Britta
Lombès, Marc
Han, Julie
Tran, Luan
Molinolo, Alfredo
Lanari, Claudia Lee Malvina
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROGESTERONE RECEPTOR ISOFORMS
BREAST CANCER MODELS; XENOGRAFTS
SPECIFIC ANTIBODIES
IMMUNOHISTOCHEMISTRY
topic PROGESTERONE RECEPTOR ISOFORMS
BREAST CANCER MODELS; XENOGRAFTS
SPECIFIC ANTIBODIES
IMMUNOHISTOCHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Progesterone receptors (PR) are prognostic and predictive biomarkers in hormone-dependent cancers. Twomain PR isoforms have been described, PRB and PRA, that differ only in that PRB has 164 extra N-terminalamino acids. It has been reported that several antibodies empirically exclusively recognize PRA in formalinfixedparaffin-embedded (FFPE) tissues. To confirm these findings, we used human breast cancer xenograftmodels, T47D-YA and -YB cells expressing PRA or PRB, respectively, MDA-MB-231 cells modified to synthesizePRB, and MDA-MB-231/iPRAB cells which can bi-inducibly express either PRA or PRB. Cells were injectedinto immunocompromised mice to generate tumours exclusively expressing PRA or PRB. PR isoform expressionwas verified using immunoblots. FFPE samples from the same tumours were studied by immunohistochemistryusing H-190, clone 636, clone 16, and Ab-6 anti-PR antibodies, the latter exclusively recognizing PRB. Exceptfor Ab-6, all antibodies displayed a similar staining pattern. Our results indicate that clones 16, 636, and theH-190 antibody recognize both PR isoforms. They point to the need for more stringency in evaluating thetrue specificity of purported PRA-specific antibodies as the PRA/PRB ratio may have prognostic and predictivevalue in breast cancer.
Fil: Fabris, Victoria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Abascal, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: May, María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Sequeira, Gonzalo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Jacobsen, Britta. University of Colorado Anschutz Medical Campus; Estados Unidos
Fil: Lombès, Marc. Inserm; Francia
Fil: Han, Julie. Moore´s Cancer Center; Estados Unidos
Fil: Tran, Luan. Moore´s Cancer Center; Estados Unidos
Fil: Molinolo, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Progesterone receptors (PR) are prognostic and predictive biomarkers in hormone-dependent cancers. Twomain PR isoforms have been described, PRB and PRA, that differ only in that PRB has 164 extra N-terminalamino acids. It has been reported that several antibodies empirically exclusively recognize PRA in formalinfixedparaffin-embedded (FFPE) tissues. To confirm these findings, we used human breast cancer xenograftmodels, T47D-YA and -YB cells expressing PRA or PRB, respectively, MDA-MB-231 cells modified to synthesizePRB, and MDA-MB-231/iPRAB cells which can bi-inducibly express either PRA or PRB. Cells were injectedinto immunocompromised mice to generate tumours exclusively expressing PRA or PRB. PR isoform expressionwas verified using immunoblots. FFPE samples from the same tumours were studied by immunohistochemistryusing H-190, clone 636, clone 16, and Ab-6 anti-PR antibodies, the latter exclusively recognizing PRB. Exceptfor Ab-6, all antibodies displayed a similar staining pattern. Our results indicate that clones 16, 636, and theH-190 antibody recognize both PR isoforms. They point to the need for more stringency in evaluating thetrue specificity of purported PRA-specific antibodies as the PRA/PRB ratio may have prognostic and predictivevalue in breast cancer.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37345
Fabris, Victoria Teresa; Abascal, Maria Florencia; Giulianelli, Sebastian Jesus; May, María; Sequeira, Gonzalo Ricardo; et al.; Isoform specificity of progesterone receptor antibodies; Wiley Online Library; The Journal of Pathology: Clinical Research; 3; 4; 10-2017; 227-233
2056-4538
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37345
identifier_str_mv Fabris, Victoria Teresa; Abascal, Maria Florencia; Giulianelli, Sebastian Jesus; May, María; Sequeira, Gonzalo Ricardo; et al.; Isoform specificity of progesterone receptor antibodies; Wiley Online Library; The Journal of Pathology: Clinical Research; 3; 4; 10-2017; 227-233
2056-4538
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cjp2.83/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/cjp2.83
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Online Library
publisher.none.fl_str_mv Wiley Online Library
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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