Real-time reaction monitoring by probe electrospray ionization mass spectrometry

Autores
Yu, Zhan; Chen, Lee Chuin; Erra Balsells, Rosa; Nonami, Hiroshi; Hiraoka, Kenzo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.
Fil: Yu, Zhan. Shenyang Normal University; China. University of Yamanashi; Japón
Fil: Chen, Lee Chuin. University of Yamanashi; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Nonami, Hiroshi. Ehime University; Japón
Fil: Hiraoka, Kenzo. University of Yamanashi; Japón
Materia
Mass spectrometry
PESI-MS
PESI-MS reaction monitoring
PESI-MS at real time
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99310
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99310
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Real-time reaction monitoring by probe electrospray ionization mass spectrometryYu, ZhanChen, Lee ChuinErra Balsells, RosaNonami, HiroshiHiraoka, KenzoMass spectrometryPESI-MSPESI-MS reaction monitoringPESI-MS at real timehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.Fil: Yu, Zhan. Shenyang Normal University; China. University of Yamanashi; JapónFil: Chen, Lee Chuin. University of Yamanashi; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Nonami, Hiroshi. Ehime University; JapónFil: Hiraoka, Kenzo. University of Yamanashi; JapónJohn Wiley & Sons Ltd2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99310Yu, Zhan; Chen, Lee Chuin; Erra Balsells, Rosa; Nonami, Hiroshi; Hiraoka, Kenzo; Real-time reaction monitoring by probe electrospray ionization mass spectrometry; John Wiley & Sons Ltd; Rapid Communications in Mass Spectrometry; 24; 11; 6-2010; 1507-15130951-4198CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/rcm.4556info:eu-repo/semantics/altIdentifier/doi/10.1002/rcm.4556info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:18Zoai:ri.conicet.gov.ar:11336/99310instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:18.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Real-time reaction monitoring by probe electrospray ionization mass spectrometry
title Real-time reaction monitoring by probe electrospray ionization mass spectrometry
spellingShingle Real-time reaction monitoring by probe electrospray ionization mass spectrometry
Yu, Zhan
Mass spectrometry
PESI-MS
PESI-MS reaction monitoring
PESI-MS at real time
title_short Real-time reaction monitoring by probe electrospray ionization mass spectrometry
title_full Real-time reaction monitoring by probe electrospray ionization mass spectrometry
title_fullStr Real-time reaction monitoring by probe electrospray ionization mass spectrometry
title_full_unstemmed Real-time reaction monitoring by probe electrospray ionization mass spectrometry
title_sort Real-time reaction monitoring by probe electrospray ionization mass spectrometry
dc.creator.none.fl_str_mv Yu, Zhan
Chen, Lee Chuin
Erra Balsells, Rosa
Nonami, Hiroshi
Hiraoka, Kenzo
author Yu, Zhan
author_facet Yu, Zhan
Chen, Lee Chuin
Erra Balsells, Rosa
Nonami, Hiroshi
Hiraoka, Kenzo
author_role author
author2 Chen, Lee Chuin
Erra Balsells, Rosa
Nonami, Hiroshi
Hiraoka, Kenzo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mass spectrometry
PESI-MS
PESI-MS reaction monitoring
PESI-MS at real time
topic Mass spectrometry
PESI-MS
PESI-MS reaction monitoring
PESI-MS at real time
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.
Fil: Yu, Zhan. Shenyang Normal University; China. University of Yamanashi; Japón
Fil: Chen, Lee Chuin. University of Yamanashi; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Nonami, Hiroshi. Ehime University; Japón
Fil: Hiraoka, Kenzo. University of Yamanashi; Japón
description Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99310
Yu, Zhan; Chen, Lee Chuin; Erra Balsells, Rosa; Nonami, Hiroshi; Hiraoka, Kenzo; Real-time reaction monitoring by probe electrospray ionization mass spectrometry; John Wiley & Sons Ltd; Rapid Communications in Mass Spectrometry; 24; 11; 6-2010; 1507-1513
0951-4198
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99310
identifier_str_mv Yu, Zhan; Chen, Lee Chuin; Erra Balsells, Rosa; Nonami, Hiroshi; Hiraoka, Kenzo; Real-time reaction monitoring by probe electrospray ionization mass spectrometry; John Wiley & Sons Ltd; Rapid Communications in Mass Spectrometry; 24; 11; 6-2010; 1507-1513
0951-4198
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/rcm.4556
info:eu-repo/semantics/altIdentifier/doi/10.1002/rcm.4556
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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