Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus

Autores
Ponsone, Maria Lorena; Kuhn, Yamila Gisele; Schmidt Heydt, M.; Geisen, R.; Chulze, Sofia Noemi
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a previous study, it was demonstrated that Kluyveromyces thermotolerans strains can reduce both growth and ochratoxin A (OTA) accumulation by Aspergillus section Nigri strains. There is no information about the mechanisms related to this reduction. A viable hypothesis can be that the presence of biocontrol agents can affect OTA biosynthesis by influencing the transcriptional activity of the polyketide synthase (pks) gene, one of the key enzymes in the OTA biosynthetic pathway. The aims of this work were to determine the effect of two selected strains of K. thermotolerans as potential biocontrol agents and to evaluate if their presence can affect the otapks gene expression of ochratoxigenic Aspergillus and Penicillium species. Growth, OTA and ochratoxin B (OTB) biosynthesis by the fungal strains at the phenotypic and molecular levels were monitored. The results obtained showed that both K. thermotolerans strains evaluated had a strong influence on growth, OTA and OTB biosynthesis, and expression of the mycotoxin biosynthesis genes. However, no direct correlation between the influence of the biocontrol yeasts on pks gene expression, OTA and OTB production could be found. These results could indicate an inhibitory mechanism by the yeasts, which apparently involve a post-transcriptional mechanism. The data obtained could imply that the production of mycotoxins can be regarded as a kind of adaptation mechanism to environmental stress conditions by these mycotoxigenic species.
Fil: Ponsone, Maria Lorena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kuhn, Yamila Gisele. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schmidt Heydt, M.. Max Rubner Institut; Alemania
Fil: Geisen, R.. Max Rubner Institut; Alemania
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Biocontrol
Kuyveromyces Thermotolerans
Ochratoxigenic Species
Ochratoxins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22949
Acceder
id CONICETDig_9294e752af67ca41aa083e8b3fbc6d15
oai_identifier_str oai:ri.conicet.gov.ar:11336/22949
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and AspergillusPonsone, Maria LorenaKuhn, Yamila GiseleSchmidt Heydt, M.Geisen, R.Chulze, Sofia NoemiBiocontrolKuyveromyces ThermotoleransOchratoxigenic SpeciesOchratoxinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In a previous study, it was demonstrated that Kluyveromyces thermotolerans strains can reduce both growth and ochratoxin A (OTA) accumulation by Aspergillus section Nigri strains. There is no information about the mechanisms related to this reduction. A viable hypothesis can be that the presence of biocontrol agents can affect OTA biosynthesis by influencing the transcriptional activity of the polyketide synthase (pks) gene, one of the key enzymes in the OTA biosynthetic pathway. The aims of this work were to determine the effect of two selected strains of K. thermotolerans as potential biocontrol agents and to evaluate if their presence can affect the otapks gene expression of ochratoxigenic Aspergillus and Penicillium species. Growth, OTA and ochratoxin B (OTB) biosynthesis by the fungal strains at the phenotypic and molecular levels were monitored. The results obtained showed that both K. thermotolerans strains evaluated had a strong influence on growth, OTA and OTB biosynthesis, and expression of the mycotoxin biosynthesis genes. However, no direct correlation between the influence of the biocontrol yeasts on pks gene expression, OTA and OTB production could be found. These results could indicate an inhibitory mechanism by the yeasts, which apparently involve a post-transcriptional mechanism. The data obtained could imply that the production of mycotoxins can be regarded as a kind of adaptation mechanism to environmental stress conditions by these mycotoxigenic species.Fil: Ponsone, Maria Lorena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kuhn, Yamila Gisele. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schmidt Heydt, M.. Max Rubner Institut; AlemaniaFil: Geisen, R.. Max Rubner Institut; AlemaniaFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWageningen Academic Publishers2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22949Ponsone, Maria Lorena; Kuhn, Yamila Gisele; Schmidt Heydt, M.; Geisen, R.; Chulze, Sofia Noemi; Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus; Wageningen Academic Publishers; World Mycotoxin Journal; 6; 3; 6-2013; 291-2971875-07101875-0796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2012.1532info:eu-repo/semantics/altIdentifier/url/http://www.wageningenacademic.com/doi/10.3920/WMJ2012.1532info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:47Zoai:ri.conicet.gov.ar:11336/22949instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:47.978CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
title Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
spellingShingle Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
Ponsone, Maria Lorena
Biocontrol
Kuyveromyces Thermotolerans
Ochratoxigenic Species
Ochratoxins
title_short Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
title_full Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
title_fullStr Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
title_full_unstemmed Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
title_sort Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus
dc.creator.none.fl_str_mv Ponsone, Maria Lorena
Kuhn, Yamila Gisele
Schmidt Heydt, M.
Geisen, R.
Chulze, Sofia Noemi
author Ponsone, Maria Lorena
author_facet Ponsone, Maria Lorena
Kuhn, Yamila Gisele
Schmidt Heydt, M.
Geisen, R.
Chulze, Sofia Noemi
author_role author
author2 Kuhn, Yamila Gisele
Schmidt Heydt, M.
Geisen, R.
Chulze, Sofia Noemi
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biocontrol
Kuyveromyces Thermotolerans
Ochratoxigenic Species
Ochratoxins
topic Biocontrol
Kuyveromyces Thermotolerans
Ochratoxigenic Species
Ochratoxins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In a previous study, it was demonstrated that Kluyveromyces thermotolerans strains can reduce both growth and ochratoxin A (OTA) accumulation by Aspergillus section Nigri strains. There is no information about the mechanisms related to this reduction. A viable hypothesis can be that the presence of biocontrol agents can affect OTA biosynthesis by influencing the transcriptional activity of the polyketide synthase (pks) gene, one of the key enzymes in the OTA biosynthetic pathway. The aims of this work were to determine the effect of two selected strains of K. thermotolerans as potential biocontrol agents and to evaluate if their presence can affect the otapks gene expression of ochratoxigenic Aspergillus and Penicillium species. Growth, OTA and ochratoxin B (OTB) biosynthesis by the fungal strains at the phenotypic and molecular levels were monitored. The results obtained showed that both K. thermotolerans strains evaluated had a strong influence on growth, OTA and OTB biosynthesis, and expression of the mycotoxin biosynthesis genes. However, no direct correlation between the influence of the biocontrol yeasts on pks gene expression, OTA and OTB production could be found. These results could indicate an inhibitory mechanism by the yeasts, which apparently involve a post-transcriptional mechanism. The data obtained could imply that the production of mycotoxins can be regarded as a kind of adaptation mechanism to environmental stress conditions by these mycotoxigenic species.
Fil: Ponsone, Maria Lorena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kuhn, Yamila Gisele. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schmidt Heydt, M.. Max Rubner Institut; Alemania
Fil: Geisen, R.. Max Rubner Institut; Alemania
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In a previous study, it was demonstrated that Kluyveromyces thermotolerans strains can reduce both growth and ochratoxin A (OTA) accumulation by Aspergillus section Nigri strains. There is no information about the mechanisms related to this reduction. A viable hypothesis can be that the presence of biocontrol agents can affect OTA biosynthesis by influencing the transcriptional activity of the polyketide synthase (pks) gene, one of the key enzymes in the OTA biosynthetic pathway. The aims of this work were to determine the effect of two selected strains of K. thermotolerans as potential biocontrol agents and to evaluate if their presence can affect the otapks gene expression of ochratoxigenic Aspergillus and Penicillium species. Growth, OTA and ochratoxin B (OTB) biosynthesis by the fungal strains at the phenotypic and molecular levels were monitored. The results obtained showed that both K. thermotolerans strains evaluated had a strong influence on growth, OTA and OTB biosynthesis, and expression of the mycotoxin biosynthesis genes. However, no direct correlation between the influence of the biocontrol yeasts on pks gene expression, OTA and OTB production could be found. These results could indicate an inhibitory mechanism by the yeasts, which apparently involve a post-transcriptional mechanism. The data obtained could imply that the production of mycotoxins can be regarded as a kind of adaptation mechanism to environmental stress conditions by these mycotoxigenic species.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22949
Ponsone, Maria Lorena; Kuhn, Yamila Gisele; Schmidt Heydt, M.; Geisen, R.; Chulze, Sofia Noemi; Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus; Wageningen Academic Publishers; World Mycotoxin Journal; 6; 3; 6-2013; 291-297
1875-0710
1875-0796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22949
identifier_str_mv Ponsone, Maria Lorena; Kuhn, Yamila Gisele; Schmidt Heydt, M.; Geisen, R.; Chulze, Sofia Noemi; Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus; Wageningen Academic Publishers; World Mycotoxin Journal; 6; 3; 6-2013; 291-297
1875-0710
1875-0796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2012.1532
info:eu-repo/semantics/altIdentifier/url/http://www.wageningenacademic.com/doi/10.3920/WMJ2012.1532
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614115545317376
score 13.070432

Publicaciones similares