Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.

Autores
Minetti, Giulia C.; Feige, Jerome N.; Bombard, Florian; Heier, Annabelle; Morvan, Fredric; Nürnberg , Bernd; Leiss, Veronika; Birnbaumer, Lutz; Glass, David J; Fornaro, Mara
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously shown that activation of G i2, an subunit of the heterotrimeric G protein complex, induces skeletal mus-cle hypertrophy and myoblast differentiation. To determine whether G i2 is required for skeletal muscle growth or regenera-tion, G i2-null mice were analyzed. G i2 knockout mice display decreased lean body mass, reduced muscle size, and impairedskeletal muscle regeneration after cardiotoxin-induced injury. Short hairpin RNA (shRNA)-mediated knockdown of G i2 insatellite cells (SCs) leads to defective satellite cell proliferation, fusion, and differentiationex vivo. The impaired differentiationis consistent with the observation that the myogenic regulatory factors MyoD and Myf5 are downregulated upon knockdown ofG i2. Interestingly, the expression of microRNA 1 (miR-1), miR-27b, and miR-206, three microRNAs that have been shown toregulate SC proliferation and differentiation, is increased by a constitutively active mutant of G i2 [G i2(Q205L)] and counter-regulated by G i2 knockdown. As for the mechanism, this study demonstrates that G i2(Q205L) regulates satellite cell differen-tiation into myotubes in a protein kinase C (PKC)- and histone deacetylase (HDAC)-dependent manner.
Fil: Minetti, Giulia C.. Novartis Campus. Basilea; Suiza
Fil: Feige, Jerome N.. Campus EPFL. Lausania; Suiza
Fil: Bombard, Florian. Novartis Campus. Basilea; Suiza
Fil: Heier, Annabelle. Novartis Campus. Basilea; Suiza
Fil: Morvan, Fredric. Novartis Campus. Basilea; Suiza
Fil: Nürnberg , Bernd. University of Tübingen. Tübingen; Alemania
Fil: Leiss, Veronika. University of Tübingen. Tübingen; Alemania
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park. North Carolina; Estados Unidos
Fil: Glass, David J. Novartis Institutes for Biomedical Research. Cambridge; Estados Unidos
Fil: Fornaro, Mara. Novartis Campus. Basilea; Suiza
Materia
Gαi2 signaling
skeletal muscle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33240
Acceder
id CONICETDig_927831fcd69f668bc39aba63a1bc0080
oai_identifier_str oai:ri.conicet.gov.ar:11336/33240
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.Minetti, Giulia C.Feige, Jerome N.Bombard, FlorianHeier, AnnabelleMorvan, FredricNürnberg , BerndLeiss, VeronikaBirnbaumer, LutzGlass, David JFornaro, MaraGαi2 signalingskeletal musclehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have previously shown that activation of G i2, an subunit of the heterotrimeric G protein complex, induces skeletal mus-cle hypertrophy and myoblast differentiation. To determine whether G i2 is required for skeletal muscle growth or regenera-tion, G i2-null mice were analyzed. G i2 knockout mice display decreased lean body mass, reduced muscle size, and impairedskeletal muscle regeneration after cardiotoxin-induced injury. Short hairpin RNA (shRNA)-mediated knockdown of G i2 insatellite cells (SCs) leads to defective satellite cell proliferation, fusion, and differentiationex vivo. The impaired differentiationis consistent with the observation that the myogenic regulatory factors MyoD and Myf5 are downregulated upon knockdown ofG i2. Interestingly, the expression of microRNA 1 (miR-1), miR-27b, and miR-206, three microRNAs that have been shown toregulate SC proliferation and differentiation, is increased by a constitutively active mutant of G i2 [G i2(Q205L)] and counter-regulated by G i2 knockdown. As for the mechanism, this study demonstrates that G i2(Q205L) regulates satellite cell differen-tiation into myotubes in a protein kinase C (PKC)- and histone deacetylase (HDAC)-dependent manner.Fil: Minetti, Giulia C.. Novartis Campus. Basilea; SuizaFil: Feige, Jerome N.. Campus EPFL. Lausania; SuizaFil: Bombard, Florian. Novartis Campus. Basilea; SuizaFil: Heier, Annabelle. Novartis Campus. Basilea; SuizaFil: Morvan, Fredric. Novartis Campus. Basilea; SuizaFil: Nürnberg , Bernd. University of Tübingen. Tübingen; AlemaniaFil: Leiss, Veronika. University of Tübingen. Tübingen; AlemaniaFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park. North Carolina; Estados UnidosFil: Glass, David J. Novartis Institutes for Biomedical Research. Cambridge; Estados UnidosFil: Fornaro, Mara. Novartis Campus. Basilea; SuizaAmerican Society for Microbiology2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33240Birnbaumer, Lutz; Leiss, Veronika; Nürnberg , Bernd; Morvan, Fredric; Heier, Annabelle; Fornaro, Mara; et al.; Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.; American Society for Microbiology; Molecular and Cellular Biology; 34; 4; 2-2014; 619-6300270-7306CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/MCB.00957-13info:eu-repo/semantics/altIdentifier/url/http://mcb.asm.org/content/34/4/619info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:04Zoai:ri.conicet.gov.ar:11336/33240instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:05.102CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
title Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
spellingShingle Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
Minetti, Giulia C.
Gαi2 signaling
skeletal muscle
title_short Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
title_full Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
title_fullStr Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
title_full_unstemmed Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
title_sort Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.
dc.creator.none.fl_str_mv Minetti, Giulia C.
Feige, Jerome N.
Bombard, Florian
Heier, Annabelle
Morvan, Fredric
Nürnberg , Bernd
Leiss, Veronika
Birnbaumer, Lutz
Glass, David J
Fornaro, Mara
author Minetti, Giulia C.
author_facet Minetti, Giulia C.
Feige, Jerome N.
Bombard, Florian
Heier, Annabelle
Morvan, Fredric
Nürnberg , Bernd
Leiss, Veronika
Birnbaumer, Lutz
Glass, David J
Fornaro, Mara
author_role author
author2 Feige, Jerome N.
Bombard, Florian
Heier, Annabelle
Morvan, Fredric
Nürnberg , Bernd
Leiss, Veronika
Birnbaumer, Lutz
Glass, David J
Fornaro, Mara
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gαi2 signaling
skeletal muscle
topic Gαi2 signaling
skeletal muscle
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have previously shown that activation of G i2, an subunit of the heterotrimeric G protein complex, induces skeletal mus-cle hypertrophy and myoblast differentiation. To determine whether G i2 is required for skeletal muscle growth or regenera-tion, G i2-null mice were analyzed. G i2 knockout mice display decreased lean body mass, reduced muscle size, and impairedskeletal muscle regeneration after cardiotoxin-induced injury. Short hairpin RNA (shRNA)-mediated knockdown of G i2 insatellite cells (SCs) leads to defective satellite cell proliferation, fusion, and differentiationex vivo. The impaired differentiationis consistent with the observation that the myogenic regulatory factors MyoD and Myf5 are downregulated upon knockdown ofG i2. Interestingly, the expression of microRNA 1 (miR-1), miR-27b, and miR-206, three microRNAs that have been shown toregulate SC proliferation and differentiation, is increased by a constitutively active mutant of G i2 [G i2(Q205L)] and counter-regulated by G i2 knockdown. As for the mechanism, this study demonstrates that G i2(Q205L) regulates satellite cell differen-tiation into myotubes in a protein kinase C (PKC)- and histone deacetylase (HDAC)-dependent manner.
Fil: Minetti, Giulia C.. Novartis Campus. Basilea; Suiza
Fil: Feige, Jerome N.. Campus EPFL. Lausania; Suiza
Fil: Bombard, Florian. Novartis Campus. Basilea; Suiza
Fil: Heier, Annabelle. Novartis Campus. Basilea; Suiza
Fil: Morvan, Fredric. Novartis Campus. Basilea; Suiza
Fil: Nürnberg , Bernd. University of Tübingen. Tübingen; Alemania
Fil: Leiss, Veronika. University of Tübingen. Tübingen; Alemania
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park. North Carolina; Estados Unidos
Fil: Glass, David J. Novartis Institutes for Biomedical Research. Cambridge; Estados Unidos
Fil: Fornaro, Mara. Novartis Campus. Basilea; Suiza
description We have previously shown that activation of G i2, an subunit of the heterotrimeric G protein complex, induces skeletal mus-cle hypertrophy and myoblast differentiation. To determine whether G i2 is required for skeletal muscle growth or regenera-tion, G i2-null mice were analyzed. G i2 knockout mice display decreased lean body mass, reduced muscle size, and impairedskeletal muscle regeneration after cardiotoxin-induced injury. Short hairpin RNA (shRNA)-mediated knockdown of G i2 insatellite cells (SCs) leads to defective satellite cell proliferation, fusion, and differentiationex vivo. The impaired differentiationis consistent with the observation that the myogenic regulatory factors MyoD and Myf5 are downregulated upon knockdown ofG i2. Interestingly, the expression of microRNA 1 (miR-1), miR-27b, and miR-206, three microRNAs that have been shown toregulate SC proliferation and differentiation, is increased by a constitutively active mutant of G i2 [G i2(Q205L)] and counter-regulated by G i2 knockdown. As for the mechanism, this study demonstrates that G i2(Q205L) regulates satellite cell differen-tiation into myotubes in a protein kinase C (PKC)- and histone deacetylase (HDAC)-dependent manner.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33240
Birnbaumer, Lutz; Leiss, Veronika; Nürnberg , Bernd; Morvan, Fredric; Heier, Annabelle; Fornaro, Mara; et al.; Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.; American Society for Microbiology; Molecular and Cellular Biology; 34; 4; 2-2014; 619-630
0270-7306
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33240
identifier_str_mv Birnbaumer, Lutz; Leiss, Veronika; Nürnberg , Bernd; Morvan, Fredric; Heier, Annabelle; Fornaro, Mara; et al.; Galfa-i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation.; American Society for Microbiology; Molecular and Cellular Biology; 34; 4; 2-2014; 619-630
0270-7306
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/MCB.00957-13
info:eu-repo/semantics/altIdentifier/url/http://mcb.asm.org/content/34/4/619
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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