Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers

Autores
Smoler, Mariano; Coceano, Giovanna; Testa, Ilaria; Bruno, Luciana; Levi, Valeria
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cytoskeleton is a complex network of interconnected biopolymers intimately involved in the generation and transmission of forces. Several mechanical properties of microtubules and actin filaments have been extensively explored in cells. In contrast, intermediate filaments (IFs) received comparatively less attention despite their central role in defining cell shape, motility and adhesion during physiological processes as well as in tumor progression. Here, we explored relevant biophysical properties of vimentin IFs in living cells combining confocal microscopy and a filament tracking routine that allows localizing filaments with ~20 nm precision. A Fourier-based analysis showed that IFs curvatures followed a thermal-like behavior characterized by an apparent persistence length (lp*) similar to that measured in aqueous solution. Additionally, we determined that certain perturbations of the cytoskeleton affect lp* and the lateral mobility of IFs as assessed in cells in which either the microtubule dynamic instability was reduced or actin filaments were partially depolymerized. Our results provide relevant clues on how vimentin IFs mechanically couple with microtubules and actin filaments in cells and support a role of this network in the response to mechanical stress.
Fil: Smoler, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Coceano, Giovanna. Royal Institute of Technology; Suecia
Fil: Testa, Ilaria. Royal Institute of Technology; Suecia
Fil: Bruno, Luciana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Calculo. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Calculo; Argentina
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
CYTOSKELETON
INTERMEDIATE FILAMENTS
LIVE-CELL MICROSCOPY
PERSISTENCE LENGTH
SINGLE FILAMENT TRACKING
VIMENTIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144191
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/144191
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymersSmoler, MarianoCoceano, GiovannaTesta, IlariaBruno, LucianaLevi, ValeriaCYTOSKELETONINTERMEDIATE FILAMENTSLIVE-CELL MICROSCOPYPERSISTENCE LENGTHSINGLE FILAMENT TRACKINGVIMENTINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The cytoskeleton is a complex network of interconnected biopolymers intimately involved in the generation and transmission of forces. Several mechanical properties of microtubules and actin filaments have been extensively explored in cells. In contrast, intermediate filaments (IFs) received comparatively less attention despite their central role in defining cell shape, motility and adhesion during physiological processes as well as in tumor progression. Here, we explored relevant biophysical properties of vimentin IFs in living cells combining confocal microscopy and a filament tracking routine that allows localizing filaments with ~20 nm precision. A Fourier-based analysis showed that IFs curvatures followed a thermal-like behavior characterized by an apparent persistence length (lp*) similar to that measured in aqueous solution. Additionally, we determined that certain perturbations of the cytoskeleton affect lp* and the lateral mobility of IFs as assessed in cells in which either the microtubule dynamic instability was reduced or actin filaments were partially depolymerized. Our results provide relevant clues on how vimentin IFs mechanically couple with microtubules and actin filaments in cells and support a role of this network in the response to mechanical stress.Fil: Smoler, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Coceano, Giovanna. Royal Institute of Technology; SueciaFil: Testa, Ilaria. Royal Institute of Technology; SueciaFil: Bruno, Luciana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Calculo. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Calculo; ArgentinaFil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaElsevier Science2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/144191Smoler, Mariano; Coceano, Giovanna; Testa, Ilaria; Bruno, Luciana; Levi, Valeria; Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1867; 8; 8-2020; 1-90167-4889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167488920300847info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamcr.2020.118726info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:19Zoai:ri.conicet.gov.ar:11336/144191instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:19.379CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
title Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
spellingShingle Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
Smoler, Mariano
CYTOSKELETON
INTERMEDIATE FILAMENTS
LIVE-CELL MICROSCOPY
PERSISTENCE LENGTH
SINGLE FILAMENT TRACKING
VIMENTIN
title_short Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
title_full Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
title_fullStr Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
title_full_unstemmed Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
title_sort Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers
dc.creator.none.fl_str_mv Smoler, Mariano
Coceano, Giovanna
Testa, Ilaria
Bruno, Luciana
Levi, Valeria
author Smoler, Mariano
author_facet Smoler, Mariano
Coceano, Giovanna
Testa, Ilaria
Bruno, Luciana
Levi, Valeria
author_role author
author2 Coceano, Giovanna
Testa, Ilaria
Bruno, Luciana
Levi, Valeria
author2_role author
author
author
author
dc.subject.none.fl_str_mv CYTOSKELETON
INTERMEDIATE FILAMENTS
LIVE-CELL MICROSCOPY
PERSISTENCE LENGTH
SINGLE FILAMENT TRACKING
VIMENTIN
topic CYTOSKELETON
INTERMEDIATE FILAMENTS
LIVE-CELL MICROSCOPY
PERSISTENCE LENGTH
SINGLE FILAMENT TRACKING
VIMENTIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The cytoskeleton is a complex network of interconnected biopolymers intimately involved in the generation and transmission of forces. Several mechanical properties of microtubules and actin filaments have been extensively explored in cells. In contrast, intermediate filaments (IFs) received comparatively less attention despite their central role in defining cell shape, motility and adhesion during physiological processes as well as in tumor progression. Here, we explored relevant biophysical properties of vimentin IFs in living cells combining confocal microscopy and a filament tracking routine that allows localizing filaments with ~20 nm precision. A Fourier-based analysis showed that IFs curvatures followed a thermal-like behavior characterized by an apparent persistence length (lp*) similar to that measured in aqueous solution. Additionally, we determined that certain perturbations of the cytoskeleton affect lp* and the lateral mobility of IFs as assessed in cells in which either the microtubule dynamic instability was reduced or actin filaments were partially depolymerized. Our results provide relevant clues on how vimentin IFs mechanically couple with microtubules and actin filaments in cells and support a role of this network in the response to mechanical stress.
Fil: Smoler, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Coceano, Giovanna. Royal Institute of Technology; Suecia
Fil: Testa, Ilaria. Royal Institute of Technology; Suecia
Fil: Bruno, Luciana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Calculo. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Calculo; Argentina
Fil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description The cytoskeleton is a complex network of interconnected biopolymers intimately involved in the generation and transmission of forces. Several mechanical properties of microtubules and actin filaments have been extensively explored in cells. In contrast, intermediate filaments (IFs) received comparatively less attention despite their central role in defining cell shape, motility and adhesion during physiological processes as well as in tumor progression. Here, we explored relevant biophysical properties of vimentin IFs in living cells combining confocal microscopy and a filament tracking routine that allows localizing filaments with ~20 nm precision. A Fourier-based analysis showed that IFs curvatures followed a thermal-like behavior characterized by an apparent persistence length (lp*) similar to that measured in aqueous solution. Additionally, we determined that certain perturbations of the cytoskeleton affect lp* and the lateral mobility of IFs as assessed in cells in which either the microtubule dynamic instability was reduced or actin filaments were partially depolymerized. Our results provide relevant clues on how vimentin IFs mechanically couple with microtubules and actin filaments in cells and support a role of this network in the response to mechanical stress.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144191
Smoler, Mariano; Coceano, Giovanna; Testa, Ilaria; Bruno, Luciana; Levi, Valeria; Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1867; 8; 8-2020; 1-9
0167-4889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144191
identifier_str_mv Smoler, Mariano; Coceano, Giovanna; Testa, Ilaria; Bruno, Luciana; Levi, Valeria; Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1867; 8; 8-2020; 1-9
0167-4889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167488920300847
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamcr.2020.118726
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613888150077440
score 13.070432

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