Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance

Autores
Srednik, Mariela Elizabeth; Grieben, Mario A.; Bentancor, Adriana Beatriz; Gentilini, Elida Raquel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as cause of bovine intramammary infection throughout the world in recent years. CoNS have been isolated from milk samples collected from cows with clinical and subclinical mastitis in several countries. Identification of mastitis pathogens is important when selecting appropriate antimicrobial therapy. Methodology: A total of 93 strains of Staphylococcus spp isolated from bovine mastitis in Argentina between 2010 and 2013 were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using the gap gene and were tested for the presence of blaZ and mecA genes by PCR and for the susceptibility to penicillin and cefoxitin by disk diffusion. Results: The most common CoNS species was S. chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%), but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in 6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxacillin MICs = 0.5 µg/ml. Discussion: CoNS are important minor mastitis pathogens and can be the cause of substantial economic losses. The presence of methicillin resistant isolates emphasizes the importance of identification of CoNS when an intramammary infection is present because of the potential risk of lateral transfer of resistant genes among staphylococcal species.
Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Grieben, Mario A.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gentilini, Elida Raquel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
ANTIMICROBIAL RESISTANCE
BLAZ GENE
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
MECA GENE
MOLECULAR IDENTIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148025
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistanceSrednik, Mariela ElizabethGrieben, Mario A.Bentancor, Adriana BeatrizGentilini, Elida RaquelANTIMICROBIAL RESISTANCEBLAZ GENEBOVINE MASTITISCOAGULASE-NEGATIVE STAPHYLOCOCCIMECA GENEMOLECULAR IDENTIFICATIONhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as cause of bovine intramammary infection throughout the world in recent years. CoNS have been isolated from milk samples collected from cows with clinical and subclinical mastitis in several countries. Identification of mastitis pathogens is important when selecting appropriate antimicrobial therapy. Methodology: A total of 93 strains of Staphylococcus spp isolated from bovine mastitis in Argentina between 2010 and 2013 were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using the gap gene and were tested for the presence of blaZ and mecA genes by PCR and for the susceptibility to penicillin and cefoxitin by disk diffusion. Results: The most common CoNS species was S. chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%), but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in 6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxacillin MICs = 0.5 µg/ml. Discussion: CoNS are important minor mastitis pathogens and can be the cause of substantial economic losses. The presence of methicillin resistant isolates emphasizes the importance of identification of CoNS when an intramammary infection is present because of the potential risk of lateral transfer of resistant genes among staphylococcal species.Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Grieben, Mario A.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Gentilini, Elida Raquel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaJournal of Infection in Developing Countries2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148025Srednik, Mariela Elizabeth; Grieben, Mario A.; Bentancor, Adriana Beatriz; Gentilini, Elida Raquel; Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 9; 9; 9-2015; 1022-10272036-65901972-2680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3855/jidc.5871info:eu-repo/semantics/altIdentifier/url/https://jidc.org/index.php/journalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:14Zoai:ri.conicet.gov.ar:11336/148025instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:14.728CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
title Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
spellingShingle Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
Srednik, Mariela Elizabeth
ANTIMICROBIAL RESISTANCE
BLAZ GENE
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
MECA GENE
MOLECULAR IDENTIFICATION
title_short Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
title_full Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
title_fullStr Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
title_full_unstemmed Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
title_sort Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
dc.creator.none.fl_str_mv Srednik, Mariela Elizabeth
Grieben, Mario A.
Bentancor, Adriana Beatriz
Gentilini, Elida Raquel
author Srednik, Mariela Elizabeth
author_facet Srednik, Mariela Elizabeth
Grieben, Mario A.
Bentancor, Adriana Beatriz
Gentilini, Elida Raquel
author_role author
author2 Grieben, Mario A.
Bentancor, Adriana Beatriz
Gentilini, Elida Raquel
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL RESISTANCE
BLAZ GENE
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
MECA GENE
MOLECULAR IDENTIFICATION
topic ANTIMICROBIAL RESISTANCE
BLAZ GENE
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
MECA GENE
MOLECULAR IDENTIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as cause of bovine intramammary infection throughout the world in recent years. CoNS have been isolated from milk samples collected from cows with clinical and subclinical mastitis in several countries. Identification of mastitis pathogens is important when selecting appropriate antimicrobial therapy. Methodology: A total of 93 strains of Staphylococcus spp isolated from bovine mastitis in Argentina between 2010 and 2013 were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using the gap gene and were tested for the presence of blaZ and mecA genes by PCR and for the susceptibility to penicillin and cefoxitin by disk diffusion. Results: The most common CoNS species was S. chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%), but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in 6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxacillin MICs = 0.5 µg/ml. Discussion: CoNS are important minor mastitis pathogens and can be the cause of substantial economic losses. The presence of methicillin resistant isolates emphasizes the importance of identification of CoNS when an intramammary infection is present because of the potential risk of lateral transfer of resistant genes among staphylococcal species.
Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Grieben, Mario A.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gentilini, Elida Raquel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as cause of bovine intramammary infection throughout the world in recent years. CoNS have been isolated from milk samples collected from cows with clinical and subclinical mastitis in several countries. Identification of mastitis pathogens is important when selecting appropriate antimicrobial therapy. Methodology: A total of 93 strains of Staphylococcus spp isolated from bovine mastitis in Argentina between 2010 and 2013 were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using the gap gene and were tested for the presence of blaZ and mecA genes by PCR and for the susceptibility to penicillin and cefoxitin by disk diffusion. Results: The most common CoNS species was S. chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%), but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in 6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxacillin MICs = 0.5 µg/ml. Discussion: CoNS are important minor mastitis pathogens and can be the cause of substantial economic losses. The presence of methicillin resistant isolates emphasizes the importance of identification of CoNS when an intramammary infection is present because of the potential risk of lateral transfer of resistant genes among staphylococcal species.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148025
Srednik, Mariela Elizabeth; Grieben, Mario A.; Bentancor, Adriana Beatriz; Gentilini, Elida Raquel; Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 9; 9; 9-2015; 1022-1027
2036-6590
1972-2680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148025
identifier_str_mv Srednik, Mariela Elizabeth; Grieben, Mario A.; Bentancor, Adriana Beatriz; Gentilini, Elida Raquel; Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 9; 9; 9-2015; 1022-1027
2036-6590
1972-2680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3855/jidc.5871
info:eu-repo/semantics/altIdentifier/url/https://jidc.org/index.php/journal
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Infection in Developing Countries
publisher.none.fl_str_mv Journal of Infection in Developing Countries
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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