Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina

Autores
Srednik, Mariela Elizabeth; Tremblay, Yannick D.N.; Labrie, Josée; Archambault, Marie; Jacques, Mario; Fernandez Cirelli, Alicia; Gentilini, Elida Raquel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.
Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Fil: Tremblay, Yannick D.N.. University of Montreal; Canadá
Fil: Labrie, Josée. University of Montreal; Canadá
Fil: Archambault, Marie. University of Montreal; Canadá. Regroupement de Recherche Pour un lait de Qualite Optimale; Canadá
Fil: Jacques, Mario. University of Montreal; Canadá. Regroupement de Recherche Pour un lait de Qualite Optimale; Canadá. Canadian Bovine Mastitis and Milk Quality Research Network; Canadá
Fil: Fernandez Cirelli, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Gentilini, Elida Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Materia
ANTIMICROBIAL RESISTANCE GENES
ARGENTINA
BIOFILM
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65449

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in ArgentinaSrednik, Mariela ElizabethTremblay, Yannick D.N.Labrie, JoséeArchambault, MarieJacques, MarioFernandez Cirelli, AliciaGentilini, Elida RaquelANTIMICROBIAL RESISTANCE GENESARGENTINABIOFILMBOVINE MASTITISCOAGULASE-NEGATIVE STAPHYLOCOCCIhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; ArgentinaFil: Tremblay, Yannick D.N.. University of Montreal; CanadáFil: Labrie, Josée. University of Montreal; CanadáFil: Archambault, Marie. University of Montreal; Canadá. Regroupement de Recherche Pour un lait de Qualite Optimale; CanadáFil: Jacques, Mario. University of Montreal; Canadá. Regroupement de Recherche Pour un lait de Qualite Optimale; Canadá. Canadian Bovine Mastitis and Milk Quality Research Network; CanadáFil: Fernandez Cirelli, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Gentilini, Elida Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; ArgentinaWiley Blackwell Publishing, Inc2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65449Srednik, Mariela Elizabeth; Tremblay, Yannick D.N.; Labrie, Josée; Archambault, Marie; Jacques, Mario; et al.; Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 364; 8; 4-2017; 1-80378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnx001info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/364/8/fnx001/2900500info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:53Zoai:ri.conicet.gov.ar:11336/65449instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:53.541CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
title Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
spellingShingle Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
Srednik, Mariela Elizabeth
ANTIMICROBIAL RESISTANCE GENES
ARGENTINA
BIOFILM
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
title_short Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
title_full Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
title_fullStr Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
title_full_unstemmed Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
title_sort Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina
dc.creator.none.fl_str_mv Srednik, Mariela Elizabeth
Tremblay, Yannick D.N.
Labrie, Josée
Archambault, Marie
Jacques, Mario
Fernandez Cirelli, Alicia
Gentilini, Elida Raquel
author Srednik, Mariela Elizabeth
author_facet Srednik, Mariela Elizabeth
Tremblay, Yannick D.N.
Labrie, Josée
Archambault, Marie
Jacques, Mario
Fernandez Cirelli, Alicia
Gentilini, Elida Raquel
author_role author
author2 Tremblay, Yannick D.N.
Labrie, Josée
Archambault, Marie
Jacques, Mario
Fernandez Cirelli, Alicia
Gentilini, Elida Raquel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL RESISTANCE GENES
ARGENTINA
BIOFILM
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
topic ANTIMICROBIAL RESISTANCE GENES
ARGENTINA
BIOFILM
BOVINE MASTITIS
COAGULASE-NEGATIVE STAPHYLOCOCCI
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.
Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Fil: Tremblay, Yannick D.N.. University of Montreal; Canadá
Fil: Labrie, Josée. University of Montreal; Canadá
Fil: Archambault, Marie. University of Montreal; Canadá. Regroupement de Recherche Pour un lait de Qualite Optimale; Canadá
Fil: Jacques, Mario. University of Montreal; Canadá. Regroupement de Recherche Pour un lait de Qualite Optimale; Canadá. Canadian Bovine Mastitis and Milk Quality Research Network; Canadá
Fil: Fernandez Cirelli, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Gentilini, Elida Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
description Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65449
Srednik, Mariela Elizabeth; Tremblay, Yannick D.N.; Labrie, Josée; Archambault, Marie; Jacques, Mario; et al.; Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 364; 8; 4-2017; 1-8
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65449
identifier_str_mv Srednik, Mariela Elizabeth; Tremblay, Yannick D.N.; Labrie, Josée; Archambault, Marie; Jacques, Mario; et al.; Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 364; 8; 4-2017; 1-8
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnx001
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/364/8/fnx001/2900500
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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