Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes
- Autores
- Mucci, Juan Sebastián; Carmona, Santiago Javier; Volcovich, Romina; Altcheh, Jaime Marcelo; Bracamonte, María Estefanía; Marco, Jorge Diego; Nielsen, Morten; Buscaglia, Carlos Andres; Agüero, Fernan Gonzalo
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Chagas Disease, caused by the protozoan Trypanosoma cruzi, is a major health and economicproblem in Latin America for which no vaccine or appropriate drugs for large-scalepublic health interventions are yet available. Accurate diagnosis is essential for the earlyidentification and follow up of vector-borne cases and to prevent transmission of the diseaseby way of blood transfusions and organ transplantation. Diagnosis is routinely performedusing serological methods, some of which require the production of parasite lysates, parasiteantigenic fractions or purified recombinant antigens. Although available serologicaltests give satisfactory results, the production of reliable reagents remains laborious andexpensive. Short peptides spanning linear B-cell epitopes have proven ideal serodiagnosticreagents in a wide range of diseases. Recently, we have conducted a large-scale screeningof T. cruzi linear B-cell epitopes using high-density peptide chips, leading to the identificationof several hundred novel sequence signatures associated to chronic Chagas Disease.Here, we performed a serological assessment of 27 selected epitopes and of their use in anovel multipeptide-based diagnostic method. A combination of 7 of these peptides werefinally evaluated in ELISA format against a panel of 199 sera samples (Chagas-positive andnegative, including sera from Leishmaniasis-positive subjects). The multipeptide formulationdisplayed a high diagnostic performance, with a sensitivity of 96.3% and a specificity of99.15%. Therefore, the use of synthetic peptides as diagnostic tools are an attractive alternativein Chagas´ disease diagnosis.
Fil: Mucci, Juan Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Carmona, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Volcovich, Romina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Bracamonte, María Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Buscaglia, Carlos Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Agüero, Fernan Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina - Materia
-
Trypanosoma cruzi
serodiagnosis
peptide
antigen - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/48673
Ver los metadatos del registro completo
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Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopesMucci, Juan SebastiánCarmona, Santiago JavierVolcovich, RominaAltcheh, Jaime MarceloBracamonte, María EstefaníaMarco, Jorge DiegoNielsen, MortenBuscaglia, Carlos AndresAgüero, Fernan GonzaloTrypanosoma cruziserodiagnosispeptideantigenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chagas Disease, caused by the protozoan Trypanosoma cruzi, is a major health and economicproblem in Latin America for which no vaccine or appropriate drugs for large-scalepublic health interventions are yet available. Accurate diagnosis is essential for the earlyidentification and follow up of vector-borne cases and to prevent transmission of the diseaseby way of blood transfusions and organ transplantation. Diagnosis is routinely performedusing serological methods, some of which require the production of parasite lysates, parasiteantigenic fractions or purified recombinant antigens. Although available serologicaltests give satisfactory results, the production of reliable reagents remains laborious andexpensive. Short peptides spanning linear B-cell epitopes have proven ideal serodiagnosticreagents in a wide range of diseases. Recently, we have conducted a large-scale screeningof T. cruzi linear B-cell epitopes using high-density peptide chips, leading to the identificationof several hundred novel sequence signatures associated to chronic Chagas Disease.Here, we performed a serological assessment of 27 selected epitopes and of their use in anovel multipeptide-based diagnostic method. A combination of 7 of these peptides werefinally evaluated in ELISA format against a panel of 199 sera samples (Chagas-positive andnegative, including sera from Leishmaniasis-positive subjects). The multipeptide formulationdisplayed a high diagnostic performance, with a sensitivity of 96.3% and a specificity of99.15%. Therefore, the use of synthetic peptides as diagnostic tools are an attractive alternativein Chagas´ disease diagnosis.Fil: Mucci, Juan Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Carmona, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Volcovich, Romina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Bracamonte, María Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Buscaglia, Carlos Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Agüero, Fernan Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaPublic Library of Science2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48673Mucci, Juan Sebastián; Carmona, Santiago Javier; Volcovich, Romina; Altcheh, Jaime Marcelo; Bracamonte, María Estefanía; et al.; Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes; Public Library of Science; Neglected Tropical Diseases; 11; 10; 10-2017; 1-191935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dx.plos.org/10.1371/journal.pntd.0005972info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005972info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:13Zoai:ri.conicet.gov.ar:11336/48673instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:14.228CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
title |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
spellingShingle |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes Mucci, Juan Sebastián Trypanosoma cruzi serodiagnosis peptide antigen |
title_short |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
title_full |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
title_fullStr |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
title_full_unstemmed |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
title_sort |
Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes |
dc.creator.none.fl_str_mv |
Mucci, Juan Sebastián Carmona, Santiago Javier Volcovich, Romina Altcheh, Jaime Marcelo Bracamonte, María Estefanía Marco, Jorge Diego Nielsen, Morten Buscaglia, Carlos Andres Agüero, Fernan Gonzalo |
author |
Mucci, Juan Sebastián |
author_facet |
Mucci, Juan Sebastián Carmona, Santiago Javier Volcovich, Romina Altcheh, Jaime Marcelo Bracamonte, María Estefanía Marco, Jorge Diego Nielsen, Morten Buscaglia, Carlos Andres Agüero, Fernan Gonzalo |
author_role |
author |
author2 |
Carmona, Santiago Javier Volcovich, Romina Altcheh, Jaime Marcelo Bracamonte, María Estefanía Marco, Jorge Diego Nielsen, Morten Buscaglia, Carlos Andres Agüero, Fernan Gonzalo |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Trypanosoma cruzi serodiagnosis peptide antigen |
topic |
Trypanosoma cruzi serodiagnosis peptide antigen |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Chagas Disease, caused by the protozoan Trypanosoma cruzi, is a major health and economicproblem in Latin America for which no vaccine or appropriate drugs for large-scalepublic health interventions are yet available. Accurate diagnosis is essential for the earlyidentification and follow up of vector-borne cases and to prevent transmission of the diseaseby way of blood transfusions and organ transplantation. Diagnosis is routinely performedusing serological methods, some of which require the production of parasite lysates, parasiteantigenic fractions or purified recombinant antigens. Although available serologicaltests give satisfactory results, the production of reliable reagents remains laborious andexpensive. Short peptides spanning linear B-cell epitopes have proven ideal serodiagnosticreagents in a wide range of diseases. Recently, we have conducted a large-scale screeningof T. cruzi linear B-cell epitopes using high-density peptide chips, leading to the identificationof several hundred novel sequence signatures associated to chronic Chagas Disease.Here, we performed a serological assessment of 27 selected epitopes and of their use in anovel multipeptide-based diagnostic method. A combination of 7 of these peptides werefinally evaluated in ELISA format against a panel of 199 sera samples (Chagas-positive andnegative, including sera from Leishmaniasis-positive subjects). The multipeptide formulationdisplayed a high diagnostic performance, with a sensitivity of 96.3% and a specificity of99.15%. Therefore, the use of synthetic peptides as diagnostic tools are an attractive alternativein Chagas´ disease diagnosis. Fil: Mucci, Juan Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Carmona, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Volcovich, Romina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Bracamonte, María Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Buscaglia, Carlos Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Agüero, Fernan Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina |
description |
Chagas Disease, caused by the protozoan Trypanosoma cruzi, is a major health and economicproblem in Latin America for which no vaccine or appropriate drugs for large-scalepublic health interventions are yet available. Accurate diagnosis is essential for the earlyidentification and follow up of vector-borne cases and to prevent transmission of the diseaseby way of blood transfusions and organ transplantation. Diagnosis is routinely performedusing serological methods, some of which require the production of parasite lysates, parasiteantigenic fractions or purified recombinant antigens. Although available serologicaltests give satisfactory results, the production of reliable reagents remains laborious andexpensive. Short peptides spanning linear B-cell epitopes have proven ideal serodiagnosticreagents in a wide range of diseases. Recently, we have conducted a large-scale screeningof T. cruzi linear B-cell epitopes using high-density peptide chips, leading to the identificationof several hundred novel sequence signatures associated to chronic Chagas Disease.Here, we performed a serological assessment of 27 selected epitopes and of their use in anovel multipeptide-based diagnostic method. A combination of 7 of these peptides werefinally evaluated in ELISA format against a panel of 199 sera samples (Chagas-positive andnegative, including sera from Leishmaniasis-positive subjects). The multipeptide formulationdisplayed a high diagnostic performance, with a sensitivity of 96.3% and a specificity of99.15%. Therefore, the use of synthetic peptides as diagnostic tools are an attractive alternativein Chagas´ disease diagnosis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/48673 Mucci, Juan Sebastián; Carmona, Santiago Javier; Volcovich, Romina; Altcheh, Jaime Marcelo; Bracamonte, María Estefanía; et al.; Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes; Public Library of Science; Neglected Tropical Diseases; 11; 10; 10-2017; 1-19 1935-2735 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/48673 |
identifier_str_mv |
Mucci, Juan Sebastián; Carmona, Santiago Javier; Volcovich, Romina; Altcheh, Jaime Marcelo; Bracamonte, María Estefanía; et al.; Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of short peptidic epitopes; Public Library of Science; Neglected Tropical Diseases; 11; 10; 10-2017; 1-19 1935-2735 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://dx.plos.org/10.1371/journal.pntd.0005972 info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005972 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613736738848768 |
score |
13.070432 |