Heparin binding analysis of boar sperm and its relation with farrowing capacity

Autores
Dapino, Dora Gabriela; Teijeiro, Juan Manuel; Cane, Fernando; Marini, Patricia Estela
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
New methods for the evaluation of semen quality according to in vivo reproductive data are useful tools for identifying boars of lower fertility among individuals with standard semen parameters. In this study, indirect fluorescence microscopy was used to evaluate the heparin binding site distribution upon capacitation of sperm from eight boars arbitrarily distributed into Groups I and II according to differential farrowing rates (Group I: ≥ 70%, Group II: < 70%). Additionally, the ability of sperm to bind to solubilised zona pellucida (ZP) was assayed in the presence or absence of heparin. Samples of two individuals of Group II showed lower percentage of B pattern in relation to other individuals (P < 0.001). The number of spermatozoa attached to ZP after 2 h of incubation in capacitating conditions with heparin was significantly lower than in its absence (P < 0.0001). These results suggest that heparin binding site distribution concerning capacitation may be indicative of the availability of proteins involved in the fertilisation process, specifically at the initial sperm-oocyte recognition. Differences in heparin binding site dynamics during capacitation may help identify a subpopulation of individuals with lower fertilising capacity and normal spermiogram, which is particularly useful at high-production establishments.
Fil: Dapino, Dora Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Teijeiro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Cane, Fernando. Provincia de Santa Fe. Ministerio de Producción. Instituto de Porcinotecnia; Argentina
Fil: Marini, Patricia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
BOAR SPERM
CAPACITATION
FARROWING RATE
HEPARIN
SULPHATED GLYCOSAMINOGLYCANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180577

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network_name_str CONICET Digital (CONICET)
spelling Heparin binding analysis of boar sperm and its relation with farrowing capacityDapino, Dora GabrielaTeijeiro, Juan ManuelCane, FernandoMarini, Patricia EstelaBOAR SPERMCAPACITATIONFARROWING RATEHEPARINSULPHATED GLYCOSAMINOGLYCANShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1New methods for the evaluation of semen quality according to in vivo reproductive data are useful tools for identifying boars of lower fertility among individuals with standard semen parameters. In this study, indirect fluorescence microscopy was used to evaluate the heparin binding site distribution upon capacitation of sperm from eight boars arbitrarily distributed into Groups I and II according to differential farrowing rates (Group I: ≥ 70%, Group II: < 70%). Additionally, the ability of sperm to bind to solubilised zona pellucida (ZP) was assayed in the presence or absence of heparin. Samples of two individuals of Group II showed lower percentage of B pattern in relation to other individuals (P < 0.001). The number of spermatozoa attached to ZP after 2 h of incubation in capacitating conditions with heparin was significantly lower than in its absence (P < 0.0001). These results suggest that heparin binding site distribution concerning capacitation may be indicative of the availability of proteins involved in the fertilisation process, specifically at the initial sperm-oocyte recognition. Differences in heparin binding site dynamics during capacitation may help identify a subpopulation of individuals with lower fertilising capacity and normal spermiogram, which is particularly useful at high-production establishments.Fil: Dapino, Dora Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Teijeiro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Cane, Fernando. Provincia de Santa Fe. Ministerio de Producción. Instituto de Porcinotecnia; ArgentinaFil: Marini, Patricia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaAkadémiai Kiadó2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180577Dapino, Dora Gabriela; Teijeiro, Juan Manuel; Cane, Fernando; Marini, Patricia Estela; Heparin binding analysis of boar sperm and its relation with farrowing capacity; Akadémiai Kiadó; Acta Veterinaria Hungarica; 62; 1; 3-2014; 96-1050236-62901588-2705CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1556/AVet.2013.056info:eu-repo/semantics/altIdentifier/url/https://akjournals.com/view/journals/004/62/1/article-p96.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:29Zoai:ri.conicet.gov.ar:11336/180577instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:29.773CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heparin binding analysis of boar sperm and its relation with farrowing capacity
title Heparin binding analysis of boar sperm and its relation with farrowing capacity
spellingShingle Heparin binding analysis of boar sperm and its relation with farrowing capacity
Dapino, Dora Gabriela
BOAR SPERM
CAPACITATION
FARROWING RATE
HEPARIN
SULPHATED GLYCOSAMINOGLYCANS
title_short Heparin binding analysis of boar sperm and its relation with farrowing capacity
title_full Heparin binding analysis of boar sperm and its relation with farrowing capacity
title_fullStr Heparin binding analysis of boar sperm and its relation with farrowing capacity
title_full_unstemmed Heparin binding analysis of boar sperm and its relation with farrowing capacity
title_sort Heparin binding analysis of boar sperm and its relation with farrowing capacity
dc.creator.none.fl_str_mv Dapino, Dora Gabriela
Teijeiro, Juan Manuel
Cane, Fernando
Marini, Patricia Estela
author Dapino, Dora Gabriela
author_facet Dapino, Dora Gabriela
Teijeiro, Juan Manuel
Cane, Fernando
Marini, Patricia Estela
author_role author
author2 Teijeiro, Juan Manuel
Cane, Fernando
Marini, Patricia Estela
author2_role author
author
author
dc.subject.none.fl_str_mv BOAR SPERM
CAPACITATION
FARROWING RATE
HEPARIN
SULPHATED GLYCOSAMINOGLYCANS
topic BOAR SPERM
CAPACITATION
FARROWING RATE
HEPARIN
SULPHATED GLYCOSAMINOGLYCANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv New methods for the evaluation of semen quality according to in vivo reproductive data are useful tools for identifying boars of lower fertility among individuals with standard semen parameters. In this study, indirect fluorescence microscopy was used to evaluate the heparin binding site distribution upon capacitation of sperm from eight boars arbitrarily distributed into Groups I and II according to differential farrowing rates (Group I: ≥ 70%, Group II: < 70%). Additionally, the ability of sperm to bind to solubilised zona pellucida (ZP) was assayed in the presence or absence of heparin. Samples of two individuals of Group II showed lower percentage of B pattern in relation to other individuals (P < 0.001). The number of spermatozoa attached to ZP after 2 h of incubation in capacitating conditions with heparin was significantly lower than in its absence (P < 0.0001). These results suggest that heparin binding site distribution concerning capacitation may be indicative of the availability of proteins involved in the fertilisation process, specifically at the initial sperm-oocyte recognition. Differences in heparin binding site dynamics during capacitation may help identify a subpopulation of individuals with lower fertilising capacity and normal spermiogram, which is particularly useful at high-production establishments.
Fil: Dapino, Dora Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Teijeiro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Cane, Fernando. Provincia de Santa Fe. Ministerio de Producción. Instituto de Porcinotecnia; Argentina
Fil: Marini, Patricia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description New methods for the evaluation of semen quality according to in vivo reproductive data are useful tools for identifying boars of lower fertility among individuals with standard semen parameters. In this study, indirect fluorescence microscopy was used to evaluate the heparin binding site distribution upon capacitation of sperm from eight boars arbitrarily distributed into Groups I and II according to differential farrowing rates (Group I: ≥ 70%, Group II: < 70%). Additionally, the ability of sperm to bind to solubilised zona pellucida (ZP) was assayed in the presence or absence of heparin. Samples of two individuals of Group II showed lower percentage of B pattern in relation to other individuals (P < 0.001). The number of spermatozoa attached to ZP after 2 h of incubation in capacitating conditions with heparin was significantly lower than in its absence (P < 0.0001). These results suggest that heparin binding site distribution concerning capacitation may be indicative of the availability of proteins involved in the fertilisation process, specifically at the initial sperm-oocyte recognition. Differences in heparin binding site dynamics during capacitation may help identify a subpopulation of individuals with lower fertilising capacity and normal spermiogram, which is particularly useful at high-production establishments.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180577
Dapino, Dora Gabriela; Teijeiro, Juan Manuel; Cane, Fernando; Marini, Patricia Estela; Heparin binding analysis of boar sperm and its relation with farrowing capacity; Akadémiai Kiadó; Acta Veterinaria Hungarica; 62; 1; 3-2014; 96-105
0236-6290
1588-2705
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180577
identifier_str_mv Dapino, Dora Gabriela; Teijeiro, Juan Manuel; Cane, Fernando; Marini, Patricia Estela; Heparin binding analysis of boar sperm and its relation with farrowing capacity; Akadémiai Kiadó; Acta Veterinaria Hungarica; 62; 1; 3-2014; 96-105
0236-6290
1588-2705
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1556/AVet.2013.056
info:eu-repo/semantics/altIdentifier/url/https://akjournals.com/view/journals/004/62/1/article-p96.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Akadémiai Kiadó
publisher.none.fl_str_mv Akadémiai Kiadó
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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