Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis

Autores
Ferreri, Lucas; Brayton, Kelly A.; Sondgeroth, Kerry S.; Lau, Audrey O. T.; Suarez, Carlos E.; McElwain, Terry F.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Small open reading frame (smorf) genes comprise the second largest Babesia bovis multigene family. All known 44 variant smorf genes are located in close chromosomal proximity to ves1 genes, which encode proteins that mediate cytoadhesion and contribute to immune evasion. In this study, we characterised the general topology of smorf genes and investigated the gene repertoire, transcriptional profile and SMORF expression in two distinct strains, T2Bo and Mo7. Sequence analysis using degenerate primers identified additional smorf genes in each strain and demonstrated that the smorf gene repertoire varies between strains, with conserved and unique genes in both. Smorf genes have multiple semi-conserved and variable blocks, and a large hypervariable insertion in 20 of the 44 genes defines two major branches of the family, termed smorf A and smorf B. A total of 32 smorf genes are simultaneously transcribed in T2Bo strain B. bovis merozoites obtained from deep brain tissue of an acutely infected animal. SMORF peptide-specific antiserum bound in immunoblots to multiple proteins with a range of sizes predicted by smorf genes, confirming translation of smorf gene products from these transcripts. These results indicate that the smorf multigene family is larger than previously described and demonstrate that smorf genes are expressed and are undergoing variation, both within strains and in a lineage-specific pattern independent of strain specificity. The function of these novel proteins is unknown.
Fil: Ferreri, Lucas. Washington State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brayton, Kelly A.. Washington State University; Estados Unidos
Fil: Sondgeroth, Kerry S.. Washington State University; Estados Unidos
Fil: Lau, Audrey O. T.. Washington State University; Estados Unidos
Fil: Suarez, Carlos E.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
Fil: McElwain, Terry F.. Washington State University; Estados Unidos
Materia
BABESIA BOVIS
SMORF MULTIGENE FAMILY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193793
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovisFerreri, LucasBrayton, Kelly A.Sondgeroth, Kerry S.Lau, Audrey O. T.Suarez, Carlos E.McElwain, Terry F.BABESIA BOVISSMORF MULTIGENE FAMILYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Small open reading frame (smorf) genes comprise the second largest Babesia bovis multigene family. All known 44 variant smorf genes are located in close chromosomal proximity to ves1 genes, which encode proteins that mediate cytoadhesion and contribute to immune evasion. In this study, we characterised the general topology of smorf genes and investigated the gene repertoire, transcriptional profile and SMORF expression in two distinct strains, T2Bo and Mo7. Sequence analysis using degenerate primers identified additional smorf genes in each strain and demonstrated that the smorf gene repertoire varies between strains, with conserved and unique genes in both. Smorf genes have multiple semi-conserved and variable blocks, and a large hypervariable insertion in 20 of the 44 genes defines two major branches of the family, termed smorf A and smorf B. A total of 32 smorf genes are simultaneously transcribed in T2Bo strain B. bovis merozoites obtained from deep brain tissue of an acutely infected animal. SMORF peptide-specific antiserum bound in immunoblots to multiple proteins with a range of sizes predicted by smorf genes, confirming translation of smorf gene products from these transcripts. These results indicate that the smorf multigene family is larger than previously described and demonstrate that smorf genes are expressed and are undergoing variation, both within strains and in a lineage-specific pattern independent of strain specificity. The function of these novel proteins is unknown.Fil: Ferreri, Lucas. Washington State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brayton, Kelly A.. Washington State University; Estados UnidosFil: Sondgeroth, Kerry S.. Washington State University; Estados UnidosFil: Lau, Audrey O. T.. Washington State University; Estados UnidosFil: Suarez, Carlos E.. United States Department of Agriculture. Agriculture Research Service; Estados UnidosFil: McElwain, Terry F.. Washington State University; Estados UnidosElsevier2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193793Ferreri, Lucas; Brayton, Kelly A.; Sondgeroth, Kerry S.; Lau, Audrey O. T.; Suarez, Carlos E.; et al.; Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis; Elsevier; International Journal for Parasitology; 42; 2; 11-2011; 131-1380020-7519CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751911002694info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2011.10.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:38Zoai:ri.conicet.gov.ar:11336/193793instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:38.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
title Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
spellingShingle Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
Ferreri, Lucas
BABESIA BOVIS
SMORF MULTIGENE FAMILY
title_short Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
title_full Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
title_fullStr Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
title_full_unstemmed Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
title_sort Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
dc.creator.none.fl_str_mv Ferreri, Lucas
Brayton, Kelly A.
Sondgeroth, Kerry S.
Lau, Audrey O. T.
Suarez, Carlos E.
McElwain, Terry F.
author Ferreri, Lucas
author_facet Ferreri, Lucas
Brayton, Kelly A.
Sondgeroth, Kerry S.
Lau, Audrey O. T.
Suarez, Carlos E.
McElwain, Terry F.
author_role author
author2 Brayton, Kelly A.
Sondgeroth, Kerry S.
Lau, Audrey O. T.
Suarez, Carlos E.
McElwain, Terry F.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BABESIA BOVIS
SMORF MULTIGENE FAMILY
topic BABESIA BOVIS
SMORF MULTIGENE FAMILY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Small open reading frame (smorf) genes comprise the second largest Babesia bovis multigene family. All known 44 variant smorf genes are located in close chromosomal proximity to ves1 genes, which encode proteins that mediate cytoadhesion and contribute to immune evasion. In this study, we characterised the general topology of smorf genes and investigated the gene repertoire, transcriptional profile and SMORF expression in two distinct strains, T2Bo and Mo7. Sequence analysis using degenerate primers identified additional smorf genes in each strain and demonstrated that the smorf gene repertoire varies between strains, with conserved and unique genes in both. Smorf genes have multiple semi-conserved and variable blocks, and a large hypervariable insertion in 20 of the 44 genes defines two major branches of the family, termed smorf A and smorf B. A total of 32 smorf genes are simultaneously transcribed in T2Bo strain B. bovis merozoites obtained from deep brain tissue of an acutely infected animal. SMORF peptide-specific antiserum bound in immunoblots to multiple proteins with a range of sizes predicted by smorf genes, confirming translation of smorf gene products from these transcripts. These results indicate that the smorf multigene family is larger than previously described and demonstrate that smorf genes are expressed and are undergoing variation, both within strains and in a lineage-specific pattern independent of strain specificity. The function of these novel proteins is unknown.
Fil: Ferreri, Lucas. Washington State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brayton, Kelly A.. Washington State University; Estados Unidos
Fil: Sondgeroth, Kerry S.. Washington State University; Estados Unidos
Fil: Lau, Audrey O. T.. Washington State University; Estados Unidos
Fil: Suarez, Carlos E.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
Fil: McElwain, Terry F.. Washington State University; Estados Unidos
description Small open reading frame (smorf) genes comprise the second largest Babesia bovis multigene family. All known 44 variant smorf genes are located in close chromosomal proximity to ves1 genes, which encode proteins that mediate cytoadhesion and contribute to immune evasion. In this study, we characterised the general topology of smorf genes and investigated the gene repertoire, transcriptional profile and SMORF expression in two distinct strains, T2Bo and Mo7. Sequence analysis using degenerate primers identified additional smorf genes in each strain and demonstrated that the smorf gene repertoire varies between strains, with conserved and unique genes in both. Smorf genes have multiple semi-conserved and variable blocks, and a large hypervariable insertion in 20 of the 44 genes defines two major branches of the family, termed smorf A and smorf B. A total of 32 smorf genes are simultaneously transcribed in T2Bo strain B. bovis merozoites obtained from deep brain tissue of an acutely infected animal. SMORF peptide-specific antiserum bound in immunoblots to multiple proteins with a range of sizes predicted by smorf genes, confirming translation of smorf gene products from these transcripts. These results indicate that the smorf multigene family is larger than previously described and demonstrate that smorf genes are expressed and are undergoing variation, both within strains and in a lineage-specific pattern independent of strain specificity. The function of these novel proteins is unknown.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193793
Ferreri, Lucas; Brayton, Kelly A.; Sondgeroth, Kerry S.; Lau, Audrey O. T.; Suarez, Carlos E.; et al.; Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis; Elsevier; International Journal for Parasitology; 42; 2; 11-2011; 131-138
0020-7519
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193793
identifier_str_mv Ferreri, Lucas; Brayton, Kelly A.; Sondgeroth, Kerry S.; Lau, Audrey O. T.; Suarez, Carlos E.; et al.; Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis; Elsevier; International Journal for Parasitology; 42; 2; 11-2011; 131-138
0020-7519
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751911002694
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2011.10.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613342939840512
score 13.260194

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