Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells
- Autores
- Yslas, Edith Inés; Ibarra, Luis Exequiel; Molina, María Alejandra; Rivarola, Claudia Rosana; Barbero, César Alfredo; Bertuzzi, Mabel Lucia; Rivarola, Viviana
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Abstract: Polyaniline nanoparticles (PANI-Nps) have been used in several applications; however, there are few publications related to the use in the photothermal therapy. PANI-Nps have high optical absorbance in the near-infrared region and in this wavelength range, biological systems are relatively transparent. For this reason, these materials can be used to absorb energy and to generate heat that destroys cancer cells selectively. PANI-Nps with average size of ca. 200 nm and neutral zeta potential were synthesized and characterized by DLS, SEM, and zeta potential. The kinetics of incorporation of PANI-Nps into LM2 cell line was monitored using UV–Vis spectrophotometry. The analysis of cell viability after PANI-Nps exposure shows that these nanoparticles are not cytotoxic even at high concentration and show no change in cell morphology and metabolic activity. Furthermore, we found that nanoparticle cell uptake reaches the maximum value c.a. 3 h after incubation. Cells were targeted by Pani-Nps and irradiated, resulting in significant elevation of intracellular ROS and heat production. One of the mechanisms of PANI-Nps-mediated photothermal killing of cancer cells apparently involved oxidative stress resulting in apoptotic cell death. Graphical Abstract: [Figure not available: see fulltext.]
Fil: Yslas, Edith Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina
Fil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Molina, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Barbero, César Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Bertuzzi, Mabel Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rivarola, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina - Materia
-
Cancer
Cytotoxicity
Environmental And Health Effects
Nanomedicine
Photothermal Therapy
Polyaniline Nanoparticles - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/69301
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Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cellsYslas, Edith InésIbarra, Luis ExequielMolina, María AlejandraRivarola, Claudia RosanaBarbero, César AlfredoBertuzzi, Mabel LuciaRivarola, VivianaCancerCytotoxicityEnvironmental And Health EffectsNanomedicinePhotothermal TherapyPolyaniline Nanoparticleshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Abstract: Polyaniline nanoparticles (PANI-Nps) have been used in several applications; however, there are few publications related to the use in the photothermal therapy. PANI-Nps have high optical absorbance in the near-infrared region and in this wavelength range, biological systems are relatively transparent. For this reason, these materials can be used to absorb energy and to generate heat that destroys cancer cells selectively. PANI-Nps with average size of ca. 200 nm and neutral zeta potential were synthesized and characterized by DLS, SEM, and zeta potential. The kinetics of incorporation of PANI-Nps into LM2 cell line was monitored using UV–Vis spectrophotometry. The analysis of cell viability after PANI-Nps exposure shows that these nanoparticles are not cytotoxic even at high concentration and show no change in cell morphology and metabolic activity. Furthermore, we found that nanoparticle cell uptake reaches the maximum value c.a. 3 h after incubation. Cells were targeted by Pani-Nps and irradiated, resulting in significant elevation of intracellular ROS and heat production. One of the mechanisms of PANI-Nps-mediated photothermal killing of cancer cells apparently involved oxidative stress resulting in apoptotic cell death. Graphical Abstract: [Figure not available: see fulltext.]Fil: Yslas, Edith Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; ArgentinaFil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Molina, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Barbero, César Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Bertuzzi, Mabel Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Rivarola, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69301Yslas, Edith Inés; Ibarra, Luis Exequiel; Molina, María Alejandra; Rivarola, Claudia Rosana; Barbero, César Alfredo; et al.; Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells; Springer; Journal of Nanoparticle Research; 17; 10; 10-2015; 1-151388-0764CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11051-015-3187-yinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11051-015-3187-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:19Zoai:ri.conicet.gov.ar:11336/69301instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:19.381CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
title |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
spellingShingle |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells Yslas, Edith Inés Cancer Cytotoxicity Environmental And Health Effects Nanomedicine Photothermal Therapy Polyaniline Nanoparticles |
title_short |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
title_full |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
title_fullStr |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
title_full_unstemmed |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
title_sort |
Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells |
dc.creator.none.fl_str_mv |
Yslas, Edith Inés Ibarra, Luis Exequiel Molina, María Alejandra Rivarola, Claudia Rosana Barbero, César Alfredo Bertuzzi, Mabel Lucia Rivarola, Viviana |
author |
Yslas, Edith Inés |
author_facet |
Yslas, Edith Inés Ibarra, Luis Exequiel Molina, María Alejandra Rivarola, Claudia Rosana Barbero, César Alfredo Bertuzzi, Mabel Lucia Rivarola, Viviana |
author_role |
author |
author2 |
Ibarra, Luis Exequiel Molina, María Alejandra Rivarola, Claudia Rosana Barbero, César Alfredo Bertuzzi, Mabel Lucia Rivarola, Viviana |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Cancer Cytotoxicity Environmental And Health Effects Nanomedicine Photothermal Therapy Polyaniline Nanoparticles |
topic |
Cancer Cytotoxicity Environmental And Health Effects Nanomedicine Photothermal Therapy Polyaniline Nanoparticles |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Abstract: Polyaniline nanoparticles (PANI-Nps) have been used in several applications; however, there are few publications related to the use in the photothermal therapy. PANI-Nps have high optical absorbance in the near-infrared region and in this wavelength range, biological systems are relatively transparent. For this reason, these materials can be used to absorb energy and to generate heat that destroys cancer cells selectively. PANI-Nps with average size of ca. 200 nm and neutral zeta potential were synthesized and characterized by DLS, SEM, and zeta potential. The kinetics of incorporation of PANI-Nps into LM2 cell line was monitored using UV–Vis spectrophotometry. The analysis of cell viability after PANI-Nps exposure shows that these nanoparticles are not cytotoxic even at high concentration and show no change in cell morphology and metabolic activity. Furthermore, we found that nanoparticle cell uptake reaches the maximum value c.a. 3 h after incubation. Cells were targeted by Pani-Nps and irradiated, resulting in significant elevation of intracellular ROS and heat production. One of the mechanisms of PANI-Nps-mediated photothermal killing of cancer cells apparently involved oxidative stress resulting in apoptotic cell death. Graphical Abstract: [Figure not available: see fulltext.] Fil: Yslas, Edith Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina Fil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Molina, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Barbero, César Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina Fil: Bertuzzi, Mabel Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Rivarola, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina |
description |
Abstract: Polyaniline nanoparticles (PANI-Nps) have been used in several applications; however, there are few publications related to the use in the photothermal therapy. PANI-Nps have high optical absorbance in the near-infrared region and in this wavelength range, biological systems are relatively transparent. For this reason, these materials can be used to absorb energy and to generate heat that destroys cancer cells selectively. PANI-Nps with average size of ca. 200 nm and neutral zeta potential were synthesized and characterized by DLS, SEM, and zeta potential. The kinetics of incorporation of PANI-Nps into LM2 cell line was monitored using UV–Vis spectrophotometry. The analysis of cell viability after PANI-Nps exposure shows that these nanoparticles are not cytotoxic even at high concentration and show no change in cell morphology and metabolic activity. Furthermore, we found that nanoparticle cell uptake reaches the maximum value c.a. 3 h after incubation. Cells were targeted by Pani-Nps and irradiated, resulting in significant elevation of intracellular ROS and heat production. One of the mechanisms of PANI-Nps-mediated photothermal killing of cancer cells apparently involved oxidative stress resulting in apoptotic cell death. Graphical Abstract: [Figure not available: see fulltext.] |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/69301 Yslas, Edith Inés; Ibarra, Luis Exequiel; Molina, María Alejandra; Rivarola, Claudia Rosana; Barbero, César Alfredo; et al.; Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells; Springer; Journal of Nanoparticle Research; 17; 10; 10-2015; 1-15 1388-0764 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/69301 |
identifier_str_mv |
Yslas, Edith Inés; Ibarra, Luis Exequiel; Molina, María Alejandra; Rivarola, Claudia Rosana; Barbero, César Alfredo; et al.; Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells; Springer; Journal of Nanoparticle Research; 17; 10; 10-2015; 1-15 1388-0764 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11051-015-3187-y info:eu-repo/semantics/altIdentifier/doi/10.1007/s11051-015-3187-y |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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