TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection

Autores
Pereson Moschen, Matias Javier; Badano, Maria Noel; Aloisi Zavala, Natalia Andrea; Chuit, Roberto; de Bracco, M. M. E.; Baré, Patricia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate concentrations of IL-6 and TNF-α in swab samples from individuals with symptoms compatible with COVID-19 and analyze their association with SARS-CoV-2 genome presence.The study was conducted on 127 combined nasopharyngeal and oropharyngeal swabs, referred to the laboratory for detection of SARS-CoV-2. Cytokine levels of symptomatic individuals with detectable (n = 52) or undetectable (n = 33) SARS-CoV-2 were measured. A group consisting of asymptomatic individuals and negative for SARS-CoV-2 genome (n = 42) were included to obtain cytokines? normal range. Concentrations of TNF-α (standard curve range: 0 to 500 pg/mL) and IL-6 (standard curve range: 0 to 300 pg/mL) in swabs were determined using commercial reagents based on enzyme linked immunosorbent assay (BD-Biosciences).TNF-α levels were higher in SARS-CoV-2 (+) symptomatic group (mean: 16 pg/mL, median value: 5.88 (5 to 172.1) pg/mL) compared with SARS-CoV-2 (?) symptomatic individuals (mean: 7.6 pg/mL, median: 5.00 (5 to 69.9) pg/mL) (P = 0.0009) (Fig. 1Bi). IL-6 was increased in some individuals with no significant difference between groups (mean: 33.95 pg/mL, median: 5.40 (5 to 467) pg/mL and for SARS-CoV-2 (?) mean: 30.4 pg/mL, median: 6.07 (5 to 466.6) pg/mL) (Fig. 1Bii). Considering a threshold value of 10 pg/mL for TNF-α, individuals with SARS-CoV-2 (+) were six times more likely to display increased levels of TNF-α (OR = 5.7; P = 0.006; 95% CI = 1,551 to 19,11). A correlation between TNF-α or IL-6 levels and viral load (Ct) was not found. Furthermore, age and gender were not related with cytokine concentration among SARS-CoV-2 (+) group. Focusing only on hospitalized patients, individuals with viral genome presence showed increased TNF-α values (mean: 18.7 pg/mL, median 5 pg/mL; 5 to 172.2) compared with those with absent SARS-CoV-2 genome (mean: 7.95 pg/mL, median 5 [5 to 65] pg/mL) (P = 0.028).Our findings, evaluating the concentration of TNF and IL-6 at the entry site of SARS-CoV-2, highlight the pleiotropic role of IL-6 in local inflammation process and show that TNF-α could contribute to disease evolution prognosis as a biomarker. Measurement of TNF-α in nasopharyngeal swabs at diagnosis could identify those individuals that may benefit from immunomodulatory and cytokine inhibiting therapies.
Fil: Pereson Moschen, Matias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y BioquÍmica. Instituto de Investigaciones En Bacteriología y Virología Molecular (IBaViM); Argentina
Fil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Aloisi Zavala, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Chuit, Roberto. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Epidemiológicas; Argentina
Fil: de Bracco, M. M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
CYTOKINES
IL-6
SARS-COV-2
TUMOR NECROSIS FACTOR
COVID-19
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215473
Acceder
id CONICETDig_9076db46a82fdb56dc420c3286f0cad6
oai_identifier_str oai:ri.conicet.gov.ar:11336/215473
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 InfectionPereson Moschen, Matias JavierBadano, Maria NoelAloisi Zavala, Natalia AndreaChuit, Robertode Bracco, M. M. E.Baré, PatriciaCYTOKINESIL-6SARS-COV-2TUMOR NECROSIS FACTORCOVID-19https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to evaluate concentrations of IL-6 and TNF-α in swab samples from individuals with symptoms compatible with COVID-19 and analyze their association with SARS-CoV-2 genome presence.The study was conducted on 127 combined nasopharyngeal and oropharyngeal swabs, referred to the laboratory for detection of SARS-CoV-2. Cytokine levels of symptomatic individuals with detectable (n = 52) or undetectable (n = 33) SARS-CoV-2 were measured. A group consisting of asymptomatic individuals and negative for SARS-CoV-2 genome (n = 42) were included to obtain cytokines? normal range. Concentrations of TNF-α (standard curve range: 0 to 500 pg/mL) and IL-6 (standard curve range: 0 to 300 pg/mL) in swabs were determined using commercial reagents based on enzyme linked immunosorbent assay (BD-Biosciences).TNF-α levels were higher in SARS-CoV-2 (+) symptomatic group (mean: 16 pg/mL, median value: 5.88 (5 to 172.1) pg/mL) compared with SARS-CoV-2 (?) symptomatic individuals (mean: 7.6 pg/mL, median: 5.00 (5 to 69.9) pg/mL) (P = 0.0009) (Fig. 1Bi). IL-6 was increased in some individuals with no significant difference between groups (mean: 33.95 pg/mL, median: 5.40 (5 to 467) pg/mL and for SARS-CoV-2 (?) mean: 30.4 pg/mL, median: 6.07 (5 to 466.6) pg/mL) (Fig. 1Bii). Considering a threshold value of 10 pg/mL for TNF-α, individuals with SARS-CoV-2 (+) were six times more likely to display increased levels of TNF-α (OR = 5.7; P = 0.006; 95% CI = 1,551 to 19,11). A correlation between TNF-α or IL-6 levels and viral load (Ct) was not found. Furthermore, age and gender were not related with cytokine concentration among SARS-CoV-2 (+) group. Focusing only on hospitalized patients, individuals with viral genome presence showed increased TNF-α values (mean: 18.7 pg/mL, median 5 pg/mL; 5 to 172.2) compared with those with absent SARS-CoV-2 genome (mean: 7.95 pg/mL, median 5 [5 to 65] pg/mL) (P = 0.028).Our findings, evaluating the concentration of TNF and IL-6 at the entry site of SARS-CoV-2, highlight the pleiotropic role of IL-6 in local inflammation process and show that TNF-α could contribute to disease evolution prognosis as a biomarker. Measurement of TNF-α in nasopharyngeal swabs at diagnosis could identify those individuals that may benefit from immunomodulatory and cytokine inhibiting therapies.Fil: Pereson Moschen, Matias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y BioquÍmica. Instituto de Investigaciones En Bacteriología y Virología Molecular (IBaViM); ArgentinaFil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Aloisi Zavala, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Chuit, Roberto. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Epidemiológicas; ArgentinaFil: de Bracco, M. M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaAmerican Society for Microbiology2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215473Pereson Moschen, Matias Javier; Badano, Maria Noel; Aloisi Zavala, Natalia Andrea; Chuit, Roberto; de Bracco, M. M. E.; et al.; TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection; American Society for Microbiology; Microbiology Spectrum; 10; 1; 2-2022; 1-32165-0497CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/spectrum.01411-21info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.01411-21info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:08:54Zoai:ri.conicet.gov.ar:11336/215473instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:08:54.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
title TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
spellingShingle TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
Pereson Moschen, Matias Javier
CYTOKINES
IL-6
SARS-COV-2
TUMOR NECROSIS FACTOR
COVID-19
title_short TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
title_full TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
title_fullStr TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
title_full_unstemmed TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
title_sort TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection
dc.creator.none.fl_str_mv Pereson Moschen, Matias Javier
Badano, Maria Noel
Aloisi Zavala, Natalia Andrea
Chuit, Roberto
de Bracco, M. M. E.
Baré, Patricia
author Pereson Moschen, Matias Javier
author_facet Pereson Moschen, Matias Javier
Badano, Maria Noel
Aloisi Zavala, Natalia Andrea
Chuit, Roberto
de Bracco, M. M. E.
Baré, Patricia
author_role author
author2 Badano, Maria Noel
Aloisi Zavala, Natalia Andrea
Chuit, Roberto
de Bracco, M. M. E.
Baré, Patricia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CYTOKINES
IL-6
SARS-COV-2
TUMOR NECROSIS FACTOR
COVID-19
topic CYTOKINES
IL-6
SARS-COV-2
TUMOR NECROSIS FACTOR
COVID-19
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to evaluate concentrations of IL-6 and TNF-α in swab samples from individuals with symptoms compatible with COVID-19 and analyze their association with SARS-CoV-2 genome presence.The study was conducted on 127 combined nasopharyngeal and oropharyngeal swabs, referred to the laboratory for detection of SARS-CoV-2. Cytokine levels of symptomatic individuals with detectable (n = 52) or undetectable (n = 33) SARS-CoV-2 were measured. A group consisting of asymptomatic individuals and negative for SARS-CoV-2 genome (n = 42) were included to obtain cytokines? normal range. Concentrations of TNF-α (standard curve range: 0 to 500 pg/mL) and IL-6 (standard curve range: 0 to 300 pg/mL) in swabs were determined using commercial reagents based on enzyme linked immunosorbent assay (BD-Biosciences).TNF-α levels were higher in SARS-CoV-2 (+) symptomatic group (mean: 16 pg/mL, median value: 5.88 (5 to 172.1) pg/mL) compared with SARS-CoV-2 (?) symptomatic individuals (mean: 7.6 pg/mL, median: 5.00 (5 to 69.9) pg/mL) (P = 0.0009) (Fig. 1Bi). IL-6 was increased in some individuals with no significant difference between groups (mean: 33.95 pg/mL, median: 5.40 (5 to 467) pg/mL and for SARS-CoV-2 (?) mean: 30.4 pg/mL, median: 6.07 (5 to 466.6) pg/mL) (Fig. 1Bii). Considering a threshold value of 10 pg/mL for TNF-α, individuals with SARS-CoV-2 (+) were six times more likely to display increased levels of TNF-α (OR = 5.7; P = 0.006; 95% CI = 1,551 to 19,11). A correlation between TNF-α or IL-6 levels and viral load (Ct) was not found. Furthermore, age and gender were not related with cytokine concentration among SARS-CoV-2 (+) group. Focusing only on hospitalized patients, individuals with viral genome presence showed increased TNF-α values (mean: 18.7 pg/mL, median 5 pg/mL; 5 to 172.2) compared with those with absent SARS-CoV-2 genome (mean: 7.95 pg/mL, median 5 [5 to 65] pg/mL) (P = 0.028).Our findings, evaluating the concentration of TNF and IL-6 at the entry site of SARS-CoV-2, highlight the pleiotropic role of IL-6 in local inflammation process and show that TNF-α could contribute to disease evolution prognosis as a biomarker. Measurement of TNF-α in nasopharyngeal swabs at diagnosis could identify those individuals that may benefit from immunomodulatory and cytokine inhibiting therapies.
Fil: Pereson Moschen, Matias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y BioquÍmica. Instituto de Investigaciones En Bacteriología y Virología Molecular (IBaViM); Argentina
Fil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Aloisi Zavala, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Chuit, Roberto. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Epidemiológicas; Argentina
Fil: de Bracco, M. M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description The aim of this study was to evaluate concentrations of IL-6 and TNF-α in swab samples from individuals with symptoms compatible with COVID-19 and analyze their association with SARS-CoV-2 genome presence.The study was conducted on 127 combined nasopharyngeal and oropharyngeal swabs, referred to the laboratory for detection of SARS-CoV-2. Cytokine levels of symptomatic individuals with detectable (n = 52) or undetectable (n = 33) SARS-CoV-2 were measured. A group consisting of asymptomatic individuals and negative for SARS-CoV-2 genome (n = 42) were included to obtain cytokines? normal range. Concentrations of TNF-α (standard curve range: 0 to 500 pg/mL) and IL-6 (standard curve range: 0 to 300 pg/mL) in swabs were determined using commercial reagents based on enzyme linked immunosorbent assay (BD-Biosciences).TNF-α levels were higher in SARS-CoV-2 (+) symptomatic group (mean: 16 pg/mL, median value: 5.88 (5 to 172.1) pg/mL) compared with SARS-CoV-2 (?) symptomatic individuals (mean: 7.6 pg/mL, median: 5.00 (5 to 69.9) pg/mL) (P = 0.0009) (Fig. 1Bi). IL-6 was increased in some individuals with no significant difference between groups (mean: 33.95 pg/mL, median: 5.40 (5 to 467) pg/mL and for SARS-CoV-2 (?) mean: 30.4 pg/mL, median: 6.07 (5 to 466.6) pg/mL) (Fig. 1Bii). Considering a threshold value of 10 pg/mL for TNF-α, individuals with SARS-CoV-2 (+) were six times more likely to display increased levels of TNF-α (OR = 5.7; P = 0.006; 95% CI = 1,551 to 19,11). A correlation between TNF-α or IL-6 levels and viral load (Ct) was not found. Furthermore, age and gender were not related with cytokine concentration among SARS-CoV-2 (+) group. Focusing only on hospitalized patients, individuals with viral genome presence showed increased TNF-α values (mean: 18.7 pg/mL, median 5 pg/mL; 5 to 172.2) compared with those with absent SARS-CoV-2 genome (mean: 7.95 pg/mL, median 5 [5 to 65] pg/mL) (P = 0.028).Our findings, evaluating the concentration of TNF and IL-6 at the entry site of SARS-CoV-2, highlight the pleiotropic role of IL-6 in local inflammation process and show that TNF-α could contribute to disease evolution prognosis as a biomarker. Measurement of TNF-α in nasopharyngeal swabs at diagnosis could identify those individuals that may benefit from immunomodulatory and cytokine inhibiting therapies.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215473
Pereson Moschen, Matias Javier; Badano, Maria Noel; Aloisi Zavala, Natalia Andrea; Chuit, Roberto; de Bracco, M. M. E.; et al.; TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection; American Society for Microbiology; Microbiology Spectrum; 10; 1; 2-2022; 1-3
2165-0497
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215473
identifier_str_mv Pereson Moschen, Matias Javier; Badano, Maria Noel; Aloisi Zavala, Natalia Andrea; Chuit, Roberto; de Bracco, M. M. E.; et al.; TNF-α Levels in Respiratory Samples Are Associated with SARSCoV- 2 Infection; American Society for Microbiology; Microbiology Spectrum; 10; 1; 2-2022; 1-3
2165-0497
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/spectrum.01411-21
info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.01411-21
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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