The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro
- Autores
- McCann, Cat; Quinteros, Michael; Adelugba, Ifeoluwa; Morgada, Marcos Nicolás; Castelblanco, Aida R.; Davis, Emily J.; Lanzirotti, Antonio; Hainer, Sarah J.; Vila, Alejandro Jose; Navea, Juan G.; Padilla-Benavides, Teresita
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The loading of copper (Cu) into cytochrome c oxidase (COX) in mitochondria is essential for energy production in cells. Extensive studies have been performed to characterize mitochondrial cuproenzymes that contribute to the metallation of COX, such as Sco1, Sco2, and Cox17. However, limited information is available on the upstream mechanism of Cu transport and delivery to mitochondria, especially through Cu-impermeable membranes, in mammalian cells. The mitochondrial phosphate transporter SLC25A3, also known as PiC2, binds Cu+ and transports the ion through these membranes in eukaryotic cells, ultimately aiding in the metallation of COX. We used the well-established differentiation model of primary myoblasts derived from mouse satellite cells, wherein Cu availability is necessary for growth and maturation, and showed that PiC2 is a target of MTF1, and its expression is both induced during myogenesis and favored by Cu supplementation. PiC2 deletion using CRISPR/Cas9 showed that the transporter is required for proliferation and differentiation of primary myoblasts, as both processes are delayed upon PiC2 knock-out. The effects of PiC2 deletion were rescued by the addition of Cu to the growth medium, implying the deleterious effects of PiC2 knockout in myoblasts may be in part due to a failure to deliver sufficient Cu to the mitochondria, which can be compensated by other mitochondrial cuproproteins. Co-localization and co-immunoprecipitation of PiC2 and COX also suggest that PiC2 may participate upstream in the copper delivery chain into COX, as verified by in vitro Cu+-transfer experiments. These data indicate an important role for PiC2 in both the delivery of Cu to the mitochondria and COX, favoring the differentiation of primary myoblasts.
Fil: McCann, Cat. Wesleyan University; Estados Unidos
Fil: Quinteros, Michael. Wesleyan University; Estados Unidos
Fil: Adelugba, Ifeoluwa. University of Massachussets; Estados Unidos
Fil: Morgada, Marcos Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Castelblanco, Aida R.. Skidmore College; Estados Unidos
Fil: Davis, Emily J.. Skidmore College; Estados Unidos
Fil: Lanzirotti, Antonio. University of Chicago; Estados Unidos
Fil: Hainer, Sarah J.. University of Pittsburgh; Estados Unidos
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Navea, Juan G.. Skidmore College; Estados Unidos
Fil: Padilla-Benavides, Teresita. Wesleyan University; Estados Unidos - Materia
-
COPPER TRANSPORT
CYTOCHROME C OXIDASE
MITOCHONDRIA
MTF1
PIC2
SLC25A3 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/211530
Ver los metadatos del registro completo
id |
CONICETDig_8feeaa0506a38f7c623274eb8a713b8c |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/211530 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitroMcCann, CatQuinteros, MichaelAdelugba, IfeoluwaMorgada, Marcos NicolásCastelblanco, Aida R.Davis, Emily J.Lanzirotti, AntonioHainer, Sarah J.Vila, Alejandro JoseNavea, Juan G.Padilla-Benavides, TeresitaCOPPER TRANSPORTCYTOCHROME C OXIDASEMITOCHONDRIAMTF1PIC2SLC25A3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The loading of copper (Cu) into cytochrome c oxidase (COX) in mitochondria is essential for energy production in cells. Extensive studies have been performed to characterize mitochondrial cuproenzymes that contribute to the metallation of COX, such as Sco1, Sco2, and Cox17. However, limited information is available on the upstream mechanism of Cu transport and delivery to mitochondria, especially through Cu-impermeable membranes, in mammalian cells. The mitochondrial phosphate transporter SLC25A3, also known as PiC2, binds Cu+ and transports the ion through these membranes in eukaryotic cells, ultimately aiding in the metallation of COX. We used the well-established differentiation model of primary myoblasts derived from mouse satellite cells, wherein Cu availability is necessary for growth and maturation, and showed that PiC2 is a target of MTF1, and its expression is both induced during myogenesis and favored by Cu supplementation. PiC2 deletion using CRISPR/Cas9 showed that the transporter is required for proliferation and differentiation of primary myoblasts, as both processes are delayed upon PiC2 knock-out. The effects of PiC2 deletion were rescued by the addition of Cu to the growth medium, implying the deleterious effects of PiC2 knockout in myoblasts may be in part due to a failure to deliver sufficient Cu to the mitochondria, which can be compensated by other mitochondrial cuproproteins. Co-localization and co-immunoprecipitation of PiC2 and COX also suggest that PiC2 may participate upstream in the copper delivery chain into COX, as verified by in vitro Cu+-transfer experiments. These data indicate an important role for PiC2 in both the delivery of Cu to the mitochondria and COX, favoring the differentiation of primary myoblasts.Fil: McCann, Cat. Wesleyan University; Estados UnidosFil: Quinteros, Michael. Wesleyan University; Estados UnidosFil: Adelugba, Ifeoluwa. University of Massachussets; Estados UnidosFil: Morgada, Marcos Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Castelblanco, Aida R.. Skidmore College; Estados UnidosFil: Davis, Emily J.. Skidmore College; Estados UnidosFil: Lanzirotti, Antonio. University of Chicago; Estados UnidosFil: Hainer, Sarah J.. University of Pittsburgh; Estados UnidosFil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Navea, Juan G.. Skidmore College; Estados UnidosFil: Padilla-Benavides, Teresita. Wesleyan University; Estados UnidosFrontiers Media2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211530McCann, Cat; Quinteros, Michael; Adelugba, Ifeoluwa; Morgada, Marcos Nicolás; Castelblanco, Aida R.; et al.; The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro; Frontiers Media; Frontiers in Molecular Biosciences; 9; 11-2022; 1-172296-889XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmolb.2022.1037941/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmolb.2022.1037941info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:33Zoai:ri.conicet.gov.ar:11336/211530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:33.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
title |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
spellingShingle |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro McCann, Cat COPPER TRANSPORT CYTOCHROME C OXIDASE MITOCHONDRIA MTF1 PIC2 SLC25A3 |
title_short |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
title_full |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
title_fullStr |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
title_full_unstemmed |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
title_sort |
The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro |
dc.creator.none.fl_str_mv |
McCann, Cat Quinteros, Michael Adelugba, Ifeoluwa Morgada, Marcos Nicolás Castelblanco, Aida R. Davis, Emily J. Lanzirotti, Antonio Hainer, Sarah J. Vila, Alejandro Jose Navea, Juan G. Padilla-Benavides, Teresita |
author |
McCann, Cat |
author_facet |
McCann, Cat Quinteros, Michael Adelugba, Ifeoluwa Morgada, Marcos Nicolás Castelblanco, Aida R. Davis, Emily J. Lanzirotti, Antonio Hainer, Sarah J. Vila, Alejandro Jose Navea, Juan G. Padilla-Benavides, Teresita |
author_role |
author |
author2 |
Quinteros, Michael Adelugba, Ifeoluwa Morgada, Marcos Nicolás Castelblanco, Aida R. Davis, Emily J. Lanzirotti, Antonio Hainer, Sarah J. Vila, Alejandro Jose Navea, Juan G. Padilla-Benavides, Teresita |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
COPPER TRANSPORT CYTOCHROME C OXIDASE MITOCHONDRIA MTF1 PIC2 SLC25A3 |
topic |
COPPER TRANSPORT CYTOCHROME C OXIDASE MITOCHONDRIA MTF1 PIC2 SLC25A3 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The loading of copper (Cu) into cytochrome c oxidase (COX) in mitochondria is essential for energy production in cells. Extensive studies have been performed to characterize mitochondrial cuproenzymes that contribute to the metallation of COX, such as Sco1, Sco2, and Cox17. However, limited information is available on the upstream mechanism of Cu transport and delivery to mitochondria, especially through Cu-impermeable membranes, in mammalian cells. The mitochondrial phosphate transporter SLC25A3, also known as PiC2, binds Cu+ and transports the ion through these membranes in eukaryotic cells, ultimately aiding in the metallation of COX. We used the well-established differentiation model of primary myoblasts derived from mouse satellite cells, wherein Cu availability is necessary for growth and maturation, and showed that PiC2 is a target of MTF1, and its expression is both induced during myogenesis and favored by Cu supplementation. PiC2 deletion using CRISPR/Cas9 showed that the transporter is required for proliferation and differentiation of primary myoblasts, as both processes are delayed upon PiC2 knock-out. The effects of PiC2 deletion were rescued by the addition of Cu to the growth medium, implying the deleterious effects of PiC2 knockout in myoblasts may be in part due to a failure to deliver sufficient Cu to the mitochondria, which can be compensated by other mitochondrial cuproproteins. Co-localization and co-immunoprecipitation of PiC2 and COX also suggest that PiC2 may participate upstream in the copper delivery chain into COX, as verified by in vitro Cu+-transfer experiments. These data indicate an important role for PiC2 in both the delivery of Cu to the mitochondria and COX, favoring the differentiation of primary myoblasts. Fil: McCann, Cat. Wesleyan University; Estados Unidos Fil: Quinteros, Michael. Wesleyan University; Estados Unidos Fil: Adelugba, Ifeoluwa. University of Massachussets; Estados Unidos Fil: Morgada, Marcos Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Castelblanco, Aida R.. Skidmore College; Estados Unidos Fil: Davis, Emily J.. Skidmore College; Estados Unidos Fil: Lanzirotti, Antonio. University of Chicago; Estados Unidos Fil: Hainer, Sarah J.. University of Pittsburgh; Estados Unidos Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Navea, Juan G.. Skidmore College; Estados Unidos Fil: Padilla-Benavides, Teresita. Wesleyan University; Estados Unidos |
description |
The loading of copper (Cu) into cytochrome c oxidase (COX) in mitochondria is essential for energy production in cells. Extensive studies have been performed to characterize mitochondrial cuproenzymes that contribute to the metallation of COX, such as Sco1, Sco2, and Cox17. However, limited information is available on the upstream mechanism of Cu transport and delivery to mitochondria, especially through Cu-impermeable membranes, in mammalian cells. The mitochondrial phosphate transporter SLC25A3, also known as PiC2, binds Cu+ and transports the ion through these membranes in eukaryotic cells, ultimately aiding in the metallation of COX. We used the well-established differentiation model of primary myoblasts derived from mouse satellite cells, wherein Cu availability is necessary for growth and maturation, and showed that PiC2 is a target of MTF1, and its expression is both induced during myogenesis and favored by Cu supplementation. PiC2 deletion using CRISPR/Cas9 showed that the transporter is required for proliferation and differentiation of primary myoblasts, as both processes are delayed upon PiC2 knock-out. The effects of PiC2 deletion were rescued by the addition of Cu to the growth medium, implying the deleterious effects of PiC2 knockout in myoblasts may be in part due to a failure to deliver sufficient Cu to the mitochondria, which can be compensated by other mitochondrial cuproproteins. Co-localization and co-immunoprecipitation of PiC2 and COX also suggest that PiC2 may participate upstream in the copper delivery chain into COX, as verified by in vitro Cu+-transfer experiments. These data indicate an important role for PiC2 in both the delivery of Cu to the mitochondria and COX, favoring the differentiation of primary myoblasts. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/211530 McCann, Cat; Quinteros, Michael; Adelugba, Ifeoluwa; Morgada, Marcos Nicolás; Castelblanco, Aida R.; et al.; The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro; Frontiers Media; Frontiers in Molecular Biosciences; 9; 11-2022; 1-17 2296-889X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/211530 |
identifier_str_mv |
McCann, Cat; Quinteros, Michael; Adelugba, Ifeoluwa; Morgada, Marcos Nicolás; Castelblanco, Aida R.; et al.; The mitochondrial Cu+ transporter PiC2 (SLC25A3) is a target of MTF1 and contributes to the development of skeletal muscle in vitro; Frontiers Media; Frontiers in Molecular Biosciences; 9; 11-2022; 1-17 2296-889X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmolb.2022.1037941/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fmolb.2022.1037941 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614289681285120 |
score |
13.070432 |