Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis

Autores
Fan, Lusheng; Gao, Bin; Xu, Ye; Flynn, Nora; Le, Brandon; You, Chenjiang; Li, Shaofang; Achkar, Natalia Patricia; Manavella, Pablo Andrés; Yang, Zhenbiao; Chen, Xuemei
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component hyponastic leaves 1 (HYL1) in the cytoplasm, nucleus, and dicing bodies. In aar2 mutants, abundance of nonphosphorylated HYL1, the active form of HYL1, and the number of HYL1-labeled dicing bodies are reduced. Primary miRNA (pri-miRNA) accumulation is compromised despite normal promoter activities of MIR genes in aar2 mutants. RNA decay assays show that the aar2-1 mutation leads to faster degradation of pri-miRNAs in a HYL1-dependent manner, which reveals a previously unknown and negative role of HYL1 in miRNA biogenesis. Taken together, our findings reveal a dual role of AAR2 in miRNA biogenesis and pre-messenger RNA splicing.
Fil: Fan, Lusheng. University of California; Estados Unidos
Fil: Gao, Bin. University of California; Estados Unidos
Fil: Xu, Ye. University of California; Estados Unidos
Fil: Flynn, Nora. University of California; Estados Unidos
Fil: Le, Brandon. University of California; Estados Unidos
Fil: You, Chenjiang. Fudan University; China
Fil: Li, Shaofang. Fudan University; China
Fil: Achkar, Natalia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Yang, Zhenbiao. University of California; Estados Unidos
Fil: Chen, Xuemei. University of California; Estados Unidos
Materia
AAR2
HYL1
MICRORNA
PRI-MIRNA
SE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214359
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/214359
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesisFan, LushengGao, BinXu, YeFlynn, NoraLe, BrandonYou, ChenjiangLi, ShaofangAchkar, Natalia PatriciaManavella, Pablo AndrésYang, ZhenbiaoChen, XuemeiAAR2HYL1MICRORNAPRI-MIRNASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component hyponastic leaves 1 (HYL1) in the cytoplasm, nucleus, and dicing bodies. In aar2 mutants, abundance of nonphosphorylated HYL1, the active form of HYL1, and the number of HYL1-labeled dicing bodies are reduced. Primary miRNA (pri-miRNA) accumulation is compromised despite normal promoter activities of MIR genes in aar2 mutants. RNA decay assays show that the aar2-1 mutation leads to faster degradation of pri-miRNAs in a HYL1-dependent manner, which reveals a previously unknown and negative role of HYL1 in miRNA biogenesis. Taken together, our findings reveal a dual role of AAR2 in miRNA biogenesis and pre-messenger RNA splicing.Fil: Fan, Lusheng. University of California; Estados UnidosFil: Gao, Bin. University of California; Estados UnidosFil: Xu, Ye. University of California; Estados UnidosFil: Flynn, Nora. University of California; Estados UnidosFil: Le, Brandon. University of California; Estados UnidosFil: You, Chenjiang. Fudan University; ChinaFil: Li, Shaofang. Fudan University; ChinaFil: Achkar, Natalia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Yang, Zhenbiao. University of California; Estados UnidosFil: Chen, Xuemei. University of California; Estados UnidosNational Academy of Sciences2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214359Fan, Lusheng; Gao, Bin; Xu, Ye; Flynn, Nora; Le, Brandon; et al.; Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 119; 41; 10-2022; 1-120027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.2208415119info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:28Zoai:ri.conicet.gov.ar:11336/214359instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:28.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
title Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
spellingShingle Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
Fan, Lusheng
AAR2
HYL1
MICRORNA
PRI-MIRNA
SE
title_short Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
title_full Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
title_fullStr Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
title_full_unstemmed Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
title_sort Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
dc.creator.none.fl_str_mv Fan, Lusheng
Gao, Bin
Xu, Ye
Flynn, Nora
Le, Brandon
You, Chenjiang
Li, Shaofang
Achkar, Natalia Patricia
Manavella, Pablo Andrés
Yang, Zhenbiao
Chen, Xuemei
author Fan, Lusheng
author_facet Fan, Lusheng
Gao, Bin
Xu, Ye
Flynn, Nora
Le, Brandon
You, Chenjiang
Li, Shaofang
Achkar, Natalia Patricia
Manavella, Pablo Andrés
Yang, Zhenbiao
Chen, Xuemei
author_role author
author2 Gao, Bin
Xu, Ye
Flynn, Nora
Le, Brandon
You, Chenjiang
Li, Shaofang
Achkar, Natalia Patricia
Manavella, Pablo Andrés
Yang, Zhenbiao
Chen, Xuemei
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AAR2
HYL1
MICRORNA
PRI-MIRNA
SE
topic AAR2
HYL1
MICRORNA
PRI-MIRNA
SE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component hyponastic leaves 1 (HYL1) in the cytoplasm, nucleus, and dicing bodies. In aar2 mutants, abundance of nonphosphorylated HYL1, the active form of HYL1, and the number of HYL1-labeled dicing bodies are reduced. Primary miRNA (pri-miRNA) accumulation is compromised despite normal promoter activities of MIR genes in aar2 mutants. RNA decay assays show that the aar2-1 mutation leads to faster degradation of pri-miRNAs in a HYL1-dependent manner, which reveals a previously unknown and negative role of HYL1 in miRNA biogenesis. Taken together, our findings reveal a dual role of AAR2 in miRNA biogenesis and pre-messenger RNA splicing.
Fil: Fan, Lusheng. University of California; Estados Unidos
Fil: Gao, Bin. University of California; Estados Unidos
Fil: Xu, Ye. University of California; Estados Unidos
Fil: Flynn, Nora. University of California; Estados Unidos
Fil: Le, Brandon. University of California; Estados Unidos
Fil: You, Chenjiang. Fudan University; China
Fil: Li, Shaofang. Fudan University; China
Fil: Achkar, Natalia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Yang, Zhenbiao. University of California; Estados Unidos
Fil: Chen, Xuemei. University of California; Estados Unidos
description MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component hyponastic leaves 1 (HYL1) in the cytoplasm, nucleus, and dicing bodies. In aar2 mutants, abundance of nonphosphorylated HYL1, the active form of HYL1, and the number of HYL1-labeled dicing bodies are reduced. Primary miRNA (pri-miRNA) accumulation is compromised despite normal promoter activities of MIR genes in aar2 mutants. RNA decay assays show that the aar2-1 mutation leads to faster degradation of pri-miRNAs in a HYL1-dependent manner, which reveals a previously unknown and negative role of HYL1 in miRNA biogenesis. Taken together, our findings reveal a dual role of AAR2 in miRNA biogenesis and pre-messenger RNA splicing.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214359
Fan, Lusheng; Gao, Bin; Xu, Ye; Flynn, Nora; Le, Brandon; et al.; Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 119; 41; 10-2022; 1-12
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214359
identifier_str_mv Fan, Lusheng; Gao, Bin; Xu, Ye; Flynn, Nora; Le, Brandon; et al.; Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 119; 41; 10-2022; 1-12
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.2208415119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980587708612608
score 12.993085

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