The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid s...

Autores
Park, Junghoon; Giudicatti, Axel Joel; Bader, Zein Eddin; Han, Min Kyun; Møller, Christian; Arce, Agustín Lucas; Xu, Zheng-Yi; Yang, Seong Wook; Manavella, Pablo Andrés; Yun, Dae-Jin
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The regulation of microRNA (miRNA) biogenesis is crucial for maintaining plant homeostasis under biotic and abiotic stress. The crosstalk between the RNA polymerase II (Pol-II) complex and the miRNA processing machinery has emerged as a central hub modulating transcription and cotranscriptional processing of primary miRNA transcripts (pri-miRNAs). However, it remains unclear how miRNA-specific transcriptional regulators recognize MIRNA loci. Here, we show that the Arabidopsis (Arabidopsis thaliana) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)–HISTONE DEACETYLASE9 (HDA9) complex is a conditional suppressor of miRNA biogenesis, particularly in response to abscisic acid (ABA). When treated with ABA, hos15/hda9 mutants show enhanced transcription of pri-miRNAs that is accompanied by increased processing, leading to overaccumulation of a set of mature miRNAs. Moreover, upon recognition of the nascent pri-miRNAs, the ABA–induced recruitment of the HOS15–HDA9 complex to MIRNA loci is guided by HYPONASTIC LEAVES 1 (HYL1). The HYL1–dependent recruitment of the HOS15–HDA9 complex to MIRNA loci suppresses expression of MIRNAs and processing of pri-miRNA. Most importantly, our findings indicate that nascent pri-miRNAs serve as scaffolds for recruiting transcriptional regulators, specifically to MIRNA loci. This indicates that RNA molecules can act as regulators of their own expression by causing a negative feedback loop that turns off their transcription, providing a self-buffering system.
Fil: Park, Junghoon. Konkuk University; Corea del Sur
Fil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Bader, Zein Eddin. Konkuk University; Corea del Sur
Fil: Han, Min Kyun. Yonsei University; Corea del Sur
Fil: Møller, Christian. Yonsei University; Corea del Sur
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Xu, Zheng-Yi. Northeast Normal University; China
Fil: Yang, Seong Wook. Yonsei University; Corea del Sur
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Yun, Dae-Jin. Konkuk University; Corea del Sur. Northeast Normal University; China
Materia
ARABIDOPSIS THALIANA
HOS15
HYL1
MIRNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/226734
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/226734
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signalingPark, JunghoonGiudicatti, Axel JoelBader, Zein EddinHan, Min KyunMøller, ChristianArce, Agustín LucasXu, Zheng-YiYang, Seong WookManavella, Pablo AndrésYun, Dae-JinARABIDOPSIS THALIANAHOS15HYL1MIRNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The regulation of microRNA (miRNA) biogenesis is crucial for maintaining plant homeostasis under biotic and abiotic stress. The crosstalk between the RNA polymerase II (Pol-II) complex and the miRNA processing machinery has emerged as a central hub modulating transcription and cotranscriptional processing of primary miRNA transcripts (pri-miRNAs). However, it remains unclear how miRNA-specific transcriptional regulators recognize MIRNA loci. Here, we show that the Arabidopsis (Arabidopsis thaliana) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)–HISTONE DEACETYLASE9 (HDA9) complex is a conditional suppressor of miRNA biogenesis, particularly in response to abscisic acid (ABA). When treated with ABA, hos15/hda9 mutants show enhanced transcription of pri-miRNAs that is accompanied by increased processing, leading to overaccumulation of a set of mature miRNAs. Moreover, upon recognition of the nascent pri-miRNAs, the ABA–induced recruitment of the HOS15–HDA9 complex to MIRNA loci is guided by HYPONASTIC LEAVES 1 (HYL1). The HYL1–dependent recruitment of the HOS15–HDA9 complex to MIRNA loci suppresses expression of MIRNAs and processing of pri-miRNA. Most importantly, our findings indicate that nascent pri-miRNAs serve as scaffolds for recruiting transcriptional regulators, specifically to MIRNA loci. This indicates that RNA molecules can act as regulators of their own expression by causing a negative feedback loop that turns off their transcription, providing a self-buffering system.Fil: Park, Junghoon. Konkuk University; Corea del SurFil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Bader, Zein Eddin. Konkuk University; Corea del SurFil: Han, Min Kyun. Yonsei University; Corea del SurFil: Møller, Christian. Yonsei University; Corea del SurFil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Xu, Zheng-Yi. Northeast Normal University; ChinaFil: Yang, Seong Wook. Yonsei University; Corea del SurFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Yun, Dae-Jin. Konkuk University; Corea del Sur. Northeast Normal University; ChinaAmerican Society of Plant Biologist2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/226734Park, Junghoon; Giudicatti, Axel Joel; Bader, Zein Eddin; Han, Min Kyun; Møller, Christian; et al.; The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling; American Society of Plant Biologist; Plant Cell; 35; 8; 5-2023; 2910-29281040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koad132/7169302info:eu-repo/semantics/altIdentifier/doi/10.1093/plcell/koad132info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:40Zoai:ri.conicet.gov.ar:11336/226734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:41.043CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
title The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
spellingShingle The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
Park, Junghoon
ARABIDOPSIS THALIANA
HOS15
HYL1
MIRNA
title_short The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
title_full The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
title_fullStr The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
title_full_unstemmed The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
title_sort The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling
dc.creator.none.fl_str_mv Park, Junghoon
Giudicatti, Axel Joel
Bader, Zein Eddin
Han, Min Kyun
Møller, Christian
Arce, Agustín Lucas
Xu, Zheng-Yi
Yang, Seong Wook
Manavella, Pablo Andrés
Yun, Dae-Jin
author Park, Junghoon
author_facet Park, Junghoon
Giudicatti, Axel Joel
Bader, Zein Eddin
Han, Min Kyun
Møller, Christian
Arce, Agustín Lucas
Xu, Zheng-Yi
Yang, Seong Wook
Manavella, Pablo Andrés
Yun, Dae-Jin
author_role author
author2 Giudicatti, Axel Joel
Bader, Zein Eddin
Han, Min Kyun
Møller, Christian
Arce, Agustín Lucas
Xu, Zheng-Yi
Yang, Seong Wook
Manavella, Pablo Andrés
Yun, Dae-Jin
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS THALIANA
HOS15
HYL1
MIRNA
topic ARABIDOPSIS THALIANA
HOS15
HYL1
MIRNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The regulation of microRNA (miRNA) biogenesis is crucial for maintaining plant homeostasis under biotic and abiotic stress. The crosstalk between the RNA polymerase II (Pol-II) complex and the miRNA processing machinery has emerged as a central hub modulating transcription and cotranscriptional processing of primary miRNA transcripts (pri-miRNAs). However, it remains unclear how miRNA-specific transcriptional regulators recognize MIRNA loci. Here, we show that the Arabidopsis (Arabidopsis thaliana) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)–HISTONE DEACETYLASE9 (HDA9) complex is a conditional suppressor of miRNA biogenesis, particularly in response to abscisic acid (ABA). When treated with ABA, hos15/hda9 mutants show enhanced transcription of pri-miRNAs that is accompanied by increased processing, leading to overaccumulation of a set of mature miRNAs. Moreover, upon recognition of the nascent pri-miRNAs, the ABA–induced recruitment of the HOS15–HDA9 complex to MIRNA loci is guided by HYPONASTIC LEAVES 1 (HYL1). The HYL1–dependent recruitment of the HOS15–HDA9 complex to MIRNA loci suppresses expression of MIRNAs and processing of pri-miRNA. Most importantly, our findings indicate that nascent pri-miRNAs serve as scaffolds for recruiting transcriptional regulators, specifically to MIRNA loci. This indicates that RNA molecules can act as regulators of their own expression by causing a negative feedback loop that turns off their transcription, providing a self-buffering system.
Fil: Park, Junghoon. Konkuk University; Corea del Sur
Fil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Bader, Zein Eddin. Konkuk University; Corea del Sur
Fil: Han, Min Kyun. Yonsei University; Corea del Sur
Fil: Møller, Christian. Yonsei University; Corea del Sur
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Xu, Zheng-Yi. Northeast Normal University; China
Fil: Yang, Seong Wook. Yonsei University; Corea del Sur
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Yun, Dae-Jin. Konkuk University; Corea del Sur. Northeast Normal University; China
description The regulation of microRNA (miRNA) biogenesis is crucial for maintaining plant homeostasis under biotic and abiotic stress. The crosstalk between the RNA polymerase II (Pol-II) complex and the miRNA processing machinery has emerged as a central hub modulating transcription and cotranscriptional processing of primary miRNA transcripts (pri-miRNAs). However, it remains unclear how miRNA-specific transcriptional regulators recognize MIRNA loci. Here, we show that the Arabidopsis (Arabidopsis thaliana) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)–HISTONE DEACETYLASE9 (HDA9) complex is a conditional suppressor of miRNA biogenesis, particularly in response to abscisic acid (ABA). When treated with ABA, hos15/hda9 mutants show enhanced transcription of pri-miRNAs that is accompanied by increased processing, leading to overaccumulation of a set of mature miRNAs. Moreover, upon recognition of the nascent pri-miRNAs, the ABA–induced recruitment of the HOS15–HDA9 complex to MIRNA loci is guided by HYPONASTIC LEAVES 1 (HYL1). The HYL1–dependent recruitment of the HOS15–HDA9 complex to MIRNA loci suppresses expression of MIRNAs and processing of pri-miRNA. Most importantly, our findings indicate that nascent pri-miRNAs serve as scaffolds for recruiting transcriptional regulators, specifically to MIRNA loci. This indicates that RNA molecules can act as regulators of their own expression by causing a negative feedback loop that turns off their transcription, providing a self-buffering system.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/226734
Park, Junghoon; Giudicatti, Axel Joel; Bader, Zein Eddin; Han, Min Kyun; Møller, Christian; et al.; The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling; American Society of Plant Biologist; Plant Cell; 35; 8; 5-2023; 2910-2928
1040-4651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/226734
identifier_str_mv Park, Junghoon; Giudicatti, Axel Joel; Bader, Zein Eddin; Han, Min Kyun; Møller, Christian; et al.; The high expression of osmotically responsive gene15–histone deacetylase9 complex associates with hyponastic leaves 1 to modulate microRNA expression in response to abscisic acid signaling; American Society of Plant Biologist; Plant Cell; 35; 8; 5-2023; 2910-2928
1040-4651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koad132/7169302
info:eu-repo/semantics/altIdentifier/doi/10.1093/plcell/koad132
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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