Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function

Autores
Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits
appear early in development.
Fil: Manchado Rojo, María. Universidad Politécnica de Cartagena; España
Fil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Politécnica de Cartagena; España
Fil: Roca, María José. Universidad Politécnica de Cartagena; España
Fil: Weiss, Julia. Universidad Politécnica de Cartagena; España
Fil: Marcos, Egea Cortines. Universidad Politécnica de Cartagena; España
Materia
Antirrhinum Majus
B Function
Autoregulatory Loop
Natwork Topology
Floral Size
Floral Scent
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24724

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oai_identifier_str oai:ri.conicet.gov.ar:11336/24724
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network_name_str CONICET Digital (CONICET)
spelling Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B functionManchado Rojo, MaríaDelgado Benarroch, LucianaRoca, María JoséWeiss, JuliaMarcos, Egea CortinesAntirrhinum MajusB FunctionAutoregulatory LoopNatwork TopologyFloral SizeFloral Scenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development.Fil: Manchado Rojo, María. Universidad Politécnica de Cartagena; EspañaFil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Politécnica de Cartagena; EspañaFil: Roca, María José. Universidad Politécnica de Cartagena; EspañaFil: Weiss, Julia. Universidad Politécnica de Cartagena; EspañaFil: Marcos, Egea Cortines. Universidad Politécnica de Cartagena; EspañaWiley Blackwell Publishing, Inc2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24724Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–3070960-74121365-313XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2012.05080.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.05080.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:15Zoai:ri.conicet.gov.ar:11336/24724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:15.516CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
title Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
spellingShingle Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
Manchado Rojo, María
Antirrhinum Majus
B Function
Autoregulatory Loop
Natwork Topology
Floral Size
Floral Scent
title_short Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
title_full Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
title_fullStr Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
title_full_unstemmed Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
title_sort Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
dc.creator.none.fl_str_mv Manchado Rojo, María
Delgado Benarroch, Luciana
Roca, María José
Weiss, Julia
Marcos, Egea Cortines
author Manchado Rojo, María
author_facet Manchado Rojo, María
Delgado Benarroch, Luciana
Roca, María José
Weiss, Julia
Marcos, Egea Cortines
author_role author
author2 Delgado Benarroch, Luciana
Roca, María José
Weiss, Julia
Marcos, Egea Cortines
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antirrhinum Majus
B Function
Autoregulatory Loop
Natwork Topology
Floral Size
Floral Scent
topic Antirrhinum Majus
B Function
Autoregulatory Loop
Natwork Topology
Floral Size
Floral Scent
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development.
Fil: Manchado Rojo, María. Universidad Politécnica de Cartagena; España
Fil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Politécnica de Cartagena; España
Fil: Roca, María José. Universidad Politécnica de Cartagena; España
Fil: Weiss, Julia. Universidad Politécnica de Cartagena; España
Fil: Marcos, Egea Cortines. Universidad Politécnica de Cartagena; España
description The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24724
Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–307
0960-7412
1365-313X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24724
identifier_str_mv Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–307
0960-7412
1365-313X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2012.05080.x
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.05080.x/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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