Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
- Autores
- Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits
appear early in development.
Fil: Manchado Rojo, María. Universidad Politécnica de Cartagena; España
Fil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Politécnica de Cartagena; España
Fil: Roca, María José. Universidad Politécnica de Cartagena; España
Fil: Weiss, Julia. Universidad Politécnica de Cartagena; España
Fil: Marcos, Egea Cortines. Universidad Politécnica de Cartagena; España - Materia
-
Antirrhinum Majus
B Function
Autoregulatory Loop
Natwork Topology
Floral Size
Floral Scent - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24724
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spelling |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B functionManchado Rojo, MaríaDelgado Benarroch, LucianaRoca, María JoséWeiss, JuliaMarcos, Egea CortinesAntirrhinum MajusB FunctionAutoregulatory LoopNatwork TopologyFloral SizeFloral Scenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development.Fil: Manchado Rojo, María. Universidad Politécnica de Cartagena; EspañaFil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Politécnica de Cartagena; EspañaFil: Roca, María José. Universidad Politécnica de Cartagena; EspañaFil: Weiss, Julia. Universidad Politécnica de Cartagena; EspañaFil: Marcos, Egea Cortines. Universidad Politécnica de Cartagena; EspañaWiley Blackwell Publishing, Inc2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24724Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–3070960-74121365-313XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2012.05080.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.05080.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:15Zoai:ri.conicet.gov.ar:11336/24724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:15.516CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
title |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
spellingShingle |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function Manchado Rojo, María Antirrhinum Majus B Function Autoregulatory Loop Natwork Topology Floral Size Floral Scent |
title_short |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
title_full |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
title_fullStr |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
title_full_unstemmed |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
title_sort |
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function |
dc.creator.none.fl_str_mv |
Manchado Rojo, María Delgado Benarroch, Luciana Roca, María José Weiss, Julia Marcos, Egea Cortines |
author |
Manchado Rojo, María |
author_facet |
Manchado Rojo, María Delgado Benarroch, Luciana Roca, María José Weiss, Julia Marcos, Egea Cortines |
author_role |
author |
author2 |
Delgado Benarroch, Luciana Roca, María José Weiss, Julia Marcos, Egea Cortines |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Antirrhinum Majus B Function Autoregulatory Loop Natwork Topology Floral Size Floral Scent |
topic |
Antirrhinum Majus B Function Autoregulatory Loop Natwork Topology Floral Size Floral Scent |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development. Fil: Manchado Rojo, María. Universidad Politécnica de Cartagena; España Fil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Politécnica de Cartagena; España Fil: Roca, María José. Universidad Politécnica de Cartagena; España Fil: Weiss, Julia. Universidad Politécnica de Cartagena; España Fil: Marcos, Egea Cortines. Universidad Politécnica de Cartagena; España |
description |
The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/24724 Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–307 0960-7412 1365-313X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/24724 |
identifier_str_mv |
Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–307 0960-7412 1365-313X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2012.05080.x info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.05080.x/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |