Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit

Autores
Guisoni, Nara Cristina; Monteoliva, D.; Diambra, Luis Anibal
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillations in an auto-repressive single-gene circuit operating in a small volume like a cell. We found that the cooperative binding of repressor molecules is not mandatory for a oscillatory behavior if there are enough binding sites in the CRS. These oscillations depend on an adequate balance between the CRS kinetic, and the synthesis/degradation rates of repressor molecules. This finding suggest that the multi-site CRS architecture can play a key role for oscillatory behavior of gene expression. Finally, our results can also help to synthetic biologists on the design of the promoters architecture for new genetic oscillatory circuits.
Fil: Guisoni, Nara Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Monteoliva, D.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Física; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
GENE CIRCUIT
NEGATIVE FEEDBACK
COOPERATIVITY
NOISE-INDUCED OSCILLATIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47956
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene CircuitGuisoni, Nara CristinaMonteoliva, D.Diambra, Luis AnibalGENE CIRCUITNEGATIVE FEEDBACKCOOPERATIVITYNOISE-INDUCED OSCILLATIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillations in an auto-repressive single-gene circuit operating in a small volume like a cell. We found that the cooperative binding of repressor molecules is not mandatory for a oscillatory behavior if there are enough binding sites in the CRS. These oscillations depend on an adequate balance between the CRS kinetic, and the synthesis/degradation rates of repressor molecules. This finding suggest that the multi-site CRS architecture can play a key role for oscillatory behavior of gene expression. Finally, our results can also help to synthetic biologists on the design of the promoters architecture for new genetic oscillatory circuits.Fil: Guisoni, Nara Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; ArgentinaFil: Monteoliva, D.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Física; ArgentinaFil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPublic Library of Science2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47956Guisoni, Nara Cristina; Monteoliva, D.; Diambra, Luis Anibal; Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit; Public Library of Science; Plos One; 11; 3; 3-2016; 1-12; e01510861932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151086info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0151086info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:06Zoai:ri.conicet.gov.ar:11336/47956instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:06.557CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
title Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
spellingShingle Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
Guisoni, Nara Cristina
GENE CIRCUIT
NEGATIVE FEEDBACK
COOPERATIVITY
NOISE-INDUCED OSCILLATIONS
title_short Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
title_full Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
title_fullStr Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
title_full_unstemmed Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
title_sort Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit
dc.creator.none.fl_str_mv Guisoni, Nara Cristina
Monteoliva, D.
Diambra, Luis Anibal
author Guisoni, Nara Cristina
author_facet Guisoni, Nara Cristina
Monteoliva, D.
Diambra, Luis Anibal
author_role author
author2 Monteoliva, D.
Diambra, Luis Anibal
author2_role author
author
dc.subject.none.fl_str_mv GENE CIRCUIT
NEGATIVE FEEDBACK
COOPERATIVITY
NOISE-INDUCED OSCILLATIONS
topic GENE CIRCUIT
NEGATIVE FEEDBACK
COOPERATIVITY
NOISE-INDUCED OSCILLATIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillations in an auto-repressive single-gene circuit operating in a small volume like a cell. We found that the cooperative binding of repressor molecules is not mandatory for a oscillatory behavior if there are enough binding sites in the CRS. These oscillations depend on an adequate balance between the CRS kinetic, and the synthesis/degradation rates of repressor molecules. This finding suggest that the multi-site CRS architecture can play a key role for oscillatory behavior of gene expression. Finally, our results can also help to synthetic biologists on the design of the promoters architecture for new genetic oscillatory circuits.
Fil: Guisoni, Nara Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Monteoliva, D.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Física; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillations in an auto-repressive single-gene circuit operating in a small volume like a cell. We found that the cooperative binding of repressor molecules is not mandatory for a oscillatory behavior if there are enough binding sites in the CRS. These oscillations depend on an adequate balance between the CRS kinetic, and the synthesis/degradation rates of repressor molecules. This finding suggest that the multi-site CRS architecture can play a key role for oscillatory behavior of gene expression. Finally, our results can also help to synthetic biologists on the design of the promoters architecture for new genetic oscillatory circuits.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47956
Guisoni, Nara Cristina; Monteoliva, D.; Diambra, Luis Anibal; Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit; Public Library of Science; Plos One; 11; 3; 3-2016; 1-12; e0151086
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47956
identifier_str_mv Guisoni, Nara Cristina; Monteoliva, D.; Diambra, Luis Anibal; Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit; Public Library of Science; Plos One; 11; 3; 3-2016; 1-12; e0151086
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151086
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0151086
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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