Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell

Autores
Bausero, María A.; Bharti, Ajit; Page, Diana T.; Perez, Kristen D.; Eng, Jason W. L.; Ordoñez, Susana L.; Jantschitsch, Christian; Kindas Muegge, Ingela; Ciocca, Daniel Ramon; Asea, Alexander
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The 25-kDa heat shock protein (Hsp25) is associated with various malignancies and is expressed at high levels in biopsies as well as circulating in the serum of breast cancer patients. In this study, we used RNA interference technology to silence the hsp25 gene in 4T1 breast adenocarcinoma cells, known as a poorly immunogenic, highly metastatic cell line. We demonstrate that transfection of 4T1 cells with short interference RNA-Hsp25 dramatically inhibits proliferation as compared with control transfected cells. In addition, we show that 4T1 cells transfected with short interference RNA-Hsp25 abrogates tumor migration potential by a mechanism that is in part due to the repression of matrix metalloproteinase 9 expression and a concomitant upregulation of its antagonist, tissue inhibitor metalloproteinase 1. Taken together, these fi ndings provide a model system for the study of metastatic potential of tumors and are suggestive of an earlier unrecognized role for Hsp25 in tumor migration.
Fil: Bausero, María A.. University of Boston. School of Medicine; Estados Unidos. Universidad de la República; Uruguay
Fil: Bharti, Ajit. University of Boston. School of Medicine; Estados Unidos
Fil: Page, Diana T.. University of Boston. School of Medicine; Estados Unidos
Fil: Perez, Kristen D.. University of Boston. School of Medicine; Estados Unidos. University System Health; Estados Unidos
Fil: Eng, Jason W. L.. University of Boston. School of Medicine; Estados Unidos
Fil: Ordoñez, Susana L.. University of Boston. School of Medicine; Estados Unidos. University System Health Science; Estados Unidos
Fil: Jantschitsch, Christian. University of Vienna; Austria
Fil: Kindas Muegge, Ingela. Institute of Cancer Research; Austria
Fil: Ciocca, Daniel Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Asea, Alexander. University System Health; Estados Unidos. University of Boston. School of Medicine; Estados Unidos
Materia
BREAST CARCINOMA
CHAPEROKINE
HEAT SHOCK PROTEINS
METASTASIS
SIRNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160420
Acceder
id CONICETDig_8e9df3a17582f0013eb82a8901a33594
oai_identifier_str oai:ri.conicet.gov.ar:11336/160420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cellBausero, María A.Bharti, AjitPage, Diana T.Perez, Kristen D.Eng, Jason W. L.Ordoñez, Susana L.Jantschitsch, ChristianKindas Muegge, IngelaCiocca, Daniel RamonAsea, AlexanderBREAST CARCINOMACHAPEROKINEHEAT SHOCK PROTEINSMETASTASISSIRNAhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The 25-kDa heat shock protein (Hsp25) is associated with various malignancies and is expressed at high levels in biopsies as well as circulating in the serum of breast cancer patients. In this study, we used RNA interference technology to silence the hsp25 gene in 4T1 breast adenocarcinoma cells, known as a poorly immunogenic, highly metastatic cell line. We demonstrate that transfection of 4T1 cells with short interference RNA-Hsp25 dramatically inhibits proliferation as compared with control transfected cells. In addition, we show that 4T1 cells transfected with short interference RNA-Hsp25 abrogates tumor migration potential by a mechanism that is in part due to the repression of matrix metalloproteinase 9 expression and a concomitant upregulation of its antagonist, tissue inhibitor metalloproteinase 1. Taken together, these fi ndings provide a model system for the study of metastatic potential of tumors and are suggestive of an earlier unrecognized role for Hsp25 in tumor migration.Fil: Bausero, María A.. University of Boston. School of Medicine; Estados Unidos. Universidad de la República; UruguayFil: Bharti, Ajit. University of Boston. School of Medicine; Estados UnidosFil: Page, Diana T.. University of Boston. School of Medicine; Estados UnidosFil: Perez, Kristen D.. University of Boston. School of Medicine; Estados Unidos. University System Health; Estados UnidosFil: Eng, Jason W. L.. University of Boston. School of Medicine; Estados UnidosFil: Ordoñez, Susana L.. University of Boston. School of Medicine; Estados Unidos. University System Health Science; Estados UnidosFil: Jantschitsch, Christian. University of Vienna; AustriaFil: Kindas Muegge, Ingela. Institute of Cancer Research; AustriaFil: Ciocca, Daniel Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Asea, Alexander. University System Health; Estados Unidos. University of Boston. School of Medicine; Estados UnidosKarger2005-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160420Bausero, María A.; Bharti, Ajit; Page, Diana T.; Perez, Kristen D.; Eng, Jason W. L.; et al.; Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell; Karger; Tumor Biology; 27; 1; 8-12-2005; 17-261010-42831423-0380CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000090152info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/90152info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:00Zoai:ri.conicet.gov.ar:11336/160420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:00.655CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
title Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
spellingShingle Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
Bausero, María A.
BREAST CARCINOMA
CHAPEROKINE
HEAT SHOCK PROTEINS
METASTASIS
SIRNA
title_short Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
title_full Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
title_fullStr Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
title_full_unstemmed Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
title_sort Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell
dc.creator.none.fl_str_mv Bausero, María A.
Bharti, Ajit
Page, Diana T.
Perez, Kristen D.
Eng, Jason W. L.
Ordoñez, Susana L.
Jantschitsch, Christian
Kindas Muegge, Ingela
Ciocca, Daniel Ramon
Asea, Alexander
author Bausero, María A.
author_facet Bausero, María A.
Bharti, Ajit
Page, Diana T.
Perez, Kristen D.
Eng, Jason W. L.
Ordoñez, Susana L.
Jantschitsch, Christian
Kindas Muegge, Ingela
Ciocca, Daniel Ramon
Asea, Alexander
author_role author
author2 Bharti, Ajit
Page, Diana T.
Perez, Kristen D.
Eng, Jason W. L.
Ordoñez, Susana L.
Jantschitsch, Christian
Kindas Muegge, Ingela
Ciocca, Daniel Ramon
Asea, Alexander
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BREAST CARCINOMA
CHAPEROKINE
HEAT SHOCK PROTEINS
METASTASIS
SIRNA
topic BREAST CARCINOMA
CHAPEROKINE
HEAT SHOCK PROTEINS
METASTASIS
SIRNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The 25-kDa heat shock protein (Hsp25) is associated with various malignancies and is expressed at high levels in biopsies as well as circulating in the serum of breast cancer patients. In this study, we used RNA interference technology to silence the hsp25 gene in 4T1 breast adenocarcinoma cells, known as a poorly immunogenic, highly metastatic cell line. We demonstrate that transfection of 4T1 cells with short interference RNA-Hsp25 dramatically inhibits proliferation as compared with control transfected cells. In addition, we show that 4T1 cells transfected with short interference RNA-Hsp25 abrogates tumor migration potential by a mechanism that is in part due to the repression of matrix metalloproteinase 9 expression and a concomitant upregulation of its antagonist, tissue inhibitor metalloproteinase 1. Taken together, these fi ndings provide a model system for the study of metastatic potential of tumors and are suggestive of an earlier unrecognized role for Hsp25 in tumor migration.
Fil: Bausero, María A.. University of Boston. School of Medicine; Estados Unidos. Universidad de la República; Uruguay
Fil: Bharti, Ajit. University of Boston. School of Medicine; Estados Unidos
Fil: Page, Diana T.. University of Boston. School of Medicine; Estados Unidos
Fil: Perez, Kristen D.. University of Boston. School of Medicine; Estados Unidos. University System Health; Estados Unidos
Fil: Eng, Jason W. L.. University of Boston. School of Medicine; Estados Unidos
Fil: Ordoñez, Susana L.. University of Boston. School of Medicine; Estados Unidos. University System Health Science; Estados Unidos
Fil: Jantschitsch, Christian. University of Vienna; Austria
Fil: Kindas Muegge, Ingela. Institute of Cancer Research; Austria
Fil: Ciocca, Daniel Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Asea, Alexander. University System Health; Estados Unidos. University of Boston. School of Medicine; Estados Unidos
description The 25-kDa heat shock protein (Hsp25) is associated with various malignancies and is expressed at high levels in biopsies as well as circulating in the serum of breast cancer patients. In this study, we used RNA interference technology to silence the hsp25 gene in 4T1 breast adenocarcinoma cells, known as a poorly immunogenic, highly metastatic cell line. We demonstrate that transfection of 4T1 cells with short interference RNA-Hsp25 dramatically inhibits proliferation as compared with control transfected cells. In addition, we show that 4T1 cells transfected with short interference RNA-Hsp25 abrogates tumor migration potential by a mechanism that is in part due to the repression of matrix metalloproteinase 9 expression and a concomitant upregulation of its antagonist, tissue inhibitor metalloproteinase 1. Taken together, these fi ndings provide a model system for the study of metastatic potential of tumors and are suggestive of an earlier unrecognized role for Hsp25 in tumor migration.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160420
Bausero, María A.; Bharti, Ajit; Page, Diana T.; Perez, Kristen D.; Eng, Jason W. L.; et al.; Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell; Karger; Tumor Biology; 27; 1; 8-12-2005; 17-26
1010-4283
1423-0380
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160420
identifier_str_mv Bausero, María A.; Bharti, Ajit; Page, Diana T.; Perez, Kristen D.; Eng, Jason W. L.; et al.; Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4t1 breast adenocarcinoma cell; Karger; Tumor Biology; 27; 1; 8-12-2005; 17-26
1010-4283
1423-0380
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000090152
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/90152
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614119199604736
score 13.070432

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